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7篇 您的检索式:作者名="Gottipamula"
    题名 作者 年代 出处 被引量
1Human platelet lysate is an alternative to fetal bovine serum for large-scale expansion of bone marrow-derived mesenchymal stromal cel s显示文摘Gottipamula S Sharma A Krishnamurthy S 0,,07:1
2Human platelet lysate is an alternative to fetal bovine serum for large-scale expansion of bone marrow-derived mesenchymal stromal cells显示文摘Sanjay Gottipamula Archana Sharma Sagar Krishnamurthy Anish Majumdar Raviraja Seetharam 2012Biotechnology Letters2012,,7:1
3Human platelet lysate is an alternative to fetal bovine serum for large- scale expansion of bone marrow-derived mesenchymal stro- mal cells显示文摘Gottipamula S Sharma A Krishnamurthy S 2012Biotechnol Lett2012,34,7:1
4Isolation, expansion and characterization of bone marrow-derived mesenehymal stromal cells in serum-free conditions 显示文摘Gottipamula S Ashwin KM Muttigi MS 2014Cell Tissue Res2014,356,1:1
5Human platelet lysate is an alternative to fetal bovine serum for largescale expansion of bone marrow-derived mesenchymal stromal cells显示文摘Gottipamula S Sharma A Krishnamurthy S 2012Biotechnol Lett2012,34,7:1
6Human platelet lysate is an alternative to fetal bovine serum for large- scale expansion of bone marrow-derived mesenchymal stromal ceils 显示文摘Gottipamula S Sharma A Krishnamurthy S 2012Biotechnol Lett2012,34,7:1
7The effect of human amniotic epithelial cells on urethral stricture fibroblasts显示文摘Background:Urethral stricture disease(USD)is effectively managed by buccal mucosa(BM)urethroplasty.Lack of adequate healthy BM has led to the use of autologous tissue-engineered BM grafts.Such grafts are costly,not easily scalable and recurrence of the stricture is still a problem.Hence,there is a requirement for cost-effective,scalable cells with innate antifibrotic properties which seem to be fulfilled by human amniotic epithelial cells(HAMECs).The effect of HAMECs on USD is unknown.Aim:To study the effect of HAMECs-CM on human urethral stricture fibroblast(USF)cells by using in-vitro migration assay and molecular techniques.Materials and Methods:USF cells were derived from six patients undergoing urethroplasty.HAMECs were derived from one placenta after delivery.The effect of HAMECs-CM on USF cell migration was observed using a standard in vitro scratch assay over a period of 3 days.The effect of HAMECs-CM on the expression levels of markers alpha-smooth muscle actin(α-SMA)and tissue inhibitor of metalloproteinases(TIMP-1)in USF cells was also examined.Results:The HAMECs-CM suppressed the migration of USF cells in in vitro scratch assay.The HAMECs-CM consistently downregulatedα-SMA,but not TIMP-1.Conclusions:HAMECs have shown antifibrotic activity on USF cells in this in vitro study.Relevance for Patients:HAMECs could serve as an alternative cell source for tissue-engineered urethroplasty.Sanjay Gottipamula Sudarson Sundarrajan Kumar Chokalingam K.N.Sridhar 2019Journal of Clinical & Translational Research2019,5,1:0
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