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| 1 | Role of pentoxifylline in non-alcoholic fatty liver disease in high-fat diet-induced obesity in mice显示文摘AIM:To study pentoxifylline effects in liver and adipose tissue inflammation in obese mice induced by high-fat diet(HFD).METHODS: Male swiss mice(6-wk old) were fed a highfat diet(HFD; 60% kcal from fat) or AIN-93(control diet; 15% kcal from fat) for 12 wk and received pentoxifylline intraperitoneally(100 mg/kg per day) for the last 14 d. Glucose homeostasis was evaluated by measurements of basal glucose blood levels and insulin tolerance test two days before the end of the protocol. Final body weight was assessed. Epididymal adipose tissue was collected and weighted for adiposity evaluation. Liver and adipose tissue biopsies were homogenized in solubilization buffer and cytokines were measured in supernatant by enzyme immunoassay or multiplex kit, respectively. Hepatic histopathologic analyses were performed in sections of paraformaldehyde-fixed, paraffin-embedded liver specimens stained with hematoxylin-eosin by an independent pathologist. Steatosis(macrovesicular and microvesicular), ballooning degeneration and inflammation were histopathologically determined. Triglycerides measurements were performed after lipid extraction in liver tissue. RESULTS: Pentoxifylline treatment reduced microsteatosis and tumor necrosis factor(TNF)-α in liver(156.3 ± 17.2 and 62.6 ± 7.6 pg/mL of TNF-α for non-treated and treated obese mice, respectively; P < 0.05). Serum aspartate aminotransferase levels were also reduced(23.2 ± 6.9 and 12.1 ± 1.6 U/L for nontreated and treated obese mice, respectively; P < 0.05) but had no effect on glucose homeostasis. In obese adipose tissue, pentoxifylline reduced TNF-α(106.1 ± 17.6 and 51.1 ± 9.6 pg/mL for non-treated and treated obese mice, respectively; P < 0.05) and interleukin-6(340.8 ± 51.3 and 166.6 ± 22.5 pg/mL for non-treated and treated obese mice, respectively; P < 0.05) levels; however, leptin(8.1 ± 0.7 and 23.1 ± 2.9 ng/mL for non-treated and treated lean mice, respectively; P < 0.05) and plasminogen activator inhibitor-1(600.2 ± 32.3 and 1508.6 ± 210.4 pg/mL for non-treated and treated lean mice, respectively; P < 0.05) levels increased in lean adipose tissue. TNF-α level in the liver of lean mice also increased(29.6 ± 6.6 and 75.4 ± 12.6 pg/mL for non-treated and treated lean mice, respectively; P < 0.05) while triglycerides presented a tendency to reduction.CONCLUSION: Pentoxifylline was beneficial in obese mice improving liver and adipose tissue inflammation. Unexpectedly, pentoxifylline increased pro-inflammatory markers in the liver and adipose tissue of lean mice. | Simone Coghetto Acedo Cintia Rabelo e Paiva Caria érica Martins Ferreira Gotardo José Aires Pereira José Pedrazzoli Marcelo Lima Ribeiro Alessandra Gambero | 2015 | World Journal of Hepatology2015，7，24： | 2 |
| 2 | Effects of methotrexate on inflammatory alterations induced by obesity: An in vivo and in vitro study显示文摘 | Caroline Candida DeOliveira Simone Coghetto Acedo érica Martins Ferreira Gotardo Patricia de Oliveira Carvalho Thalita Rocha José Pedrazzoli Alessandra Gambero | 2012 | 2012 (1-2)2012，，1： | 1 |
| 3 | Gradient vector flow fast geodesic active contours显示文摘 | Paragios N Mellina -Gotardo O Ramesh V | 2004 | IEEE Transactions on Pattern Analysis and Machine Intelligence2004，26，3： | 1 |
| 4 | Migration of Diethylhexyl Phthalate from PVC Bags into Intravenous Cyclosporine Solutions显示文摘 | GOTARDO M A | 2005 | Journal of Pharmaceutical and Biomedical Analysis2005，38，4： | 1 |
| 5 | Spcctrophotometric determination of formaldehyde with chromotropic acid in phosphoric acid medium assisted by microwave oven显示文摘 | GIGANTE A C GOTARDO M A TOGNOLLI J O | 2004 | Microchemical Journal2004，77，： | 1 |
| 6 | Mice that are fed a high-fat diet display increased hepcidin expression in adipose tissue显示文摘 | GOTARDO E M F SANTOS A N NIYASHIRO R A | 2013 | J Nutr Sci Vitaminol2013，59，5： | 1 |
| 7 | Immunological Tolerance to Pig-serum Partially Inhibits the Formation of Septal Fibrosis of the Liver in Capillaria hepatica infected Rats显示文摘 | Andrade RG Gotardo BM Assis BC | 2004 | Mem Inst OswaldoCruz2004，99，7： | 1 |
| 8 | Factors related to English reading performance in Children with Chinese as a first language: More evidence of cross-language transfer of phonological processing显示文摘 | Gotardo A Yan B Siegel L S | 2001 | Journal of Educational Psychology2001，93，： | 1 |
| 9 | Infliximab modifies mesenteric adipose tissue alterations and intestinal inflammation in rats with TNBS-induced colitis显示文摘 | Thayane Rodrigues Leite Clemente Aline Noronha dos Santos José Narciso Sturaro érica Martins Ferreira Gotardo Caroline Candida de Oliveira Simone Coghetto Acedo Cintia Rabelo e Paiva Caria José Pedrazzoli Marcelo Lima Ribeiro Alessandra Gambero | 2012 | Scandinavian Journal of Gastroenterology (-)2012，，8： | 1 |
| 10 | Range image segmentation into phanar and quadric surfaces using an improved robust estimator and genetic algorithm显示文摘 | Gotardo P Bellon O | 2004 | IEEE Transactions on System Man Cybernetics2004，34，6： | 1 |
| 11 | Anti-inflammatory effects of yerba maté extract ( Ilex paraguariensis ) ameliorate insulin resistance in mice with high fat diet-induced obesity显示文摘 | Demétrius P. Ar?ari Waldemar Bartchewsky Tanila W. dos Santos Karim A. Oliveira Carlorine C. DeOliveira érica M. Gotardo José Pedrazzoli Alessandra Gambero Lucio F.C. Ferraz Patricia de O. Carvalho Marcelo L. Ribeiro | 2011 | Molecular and Cellular Endocrinology2011，，2： | 1 |
| 12 | Migration of diethylhexyl phthaleate from PVC bags into intraveous cyclosporine solutions显示文摘 | Gotardo M A | | 0，，： | 1 |
| 13 | Liver transplantation and quality of life: relevance of a specific liver disease questionnaire显示文摘 | Gotardo DR Strauss E Teixeira MC | 2008 | Liver Int2008，28，1： | 1 |
| 14 | Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats显示文摘AIM:To investigate hepcidin expression,interleukin-6(IL-6)production and iron levels in the rat colon in the presence of trinitrobenzene sulfonic acid(TNBS)-induced colitis.METHODS:In rats,we evaluated the severity of colitis induced by repeated TNBS administration using macroscopic and microscopic scoring systems and myeloperoxidase activity measurements.The colonic levels of hepcidin,tumor necrosis factor alpha(TNF-α),IL-10 and IL-6 were measured by Enzyme-Linked Immunosorbent Assay,and hepcidin-25 expression and iron deposition were analyzed by immunohistochemistry and the Prussian blue reaction,respectively.Stat-3 phosphorylation was assessed by Western blot analysis.Hematological parameters,iron and transferrin levels,and transferrin saturation were also measured.Additionally,the ability of iron,pathogen-derived molecules and IL-6 to induce hepcidin expression in HT-29 cells was evaluated.RESULTS:Repeated TNBS administration to rats resulted in macroscopically and microscopically detectable colon lesions and elevated colonic myeloperoxidase activity.Hepcidin-25 protein levels were increased in colonic surface epithelia in colitic rats(10.2±4.0pg/mg protein vs 71.0±8.4 pg/mg protein,P<0.01).Elevated IL-6 levels(8.2±1.7 pg/mg protein vs 14.7±0.7 pg/mg protein,P<0.05),TNF-αlevels(1.8±1.2pg/mg protein vs 7.4±2.1 pg/mg protein,P<0.05)and Stat-3 phosphorylation were also observed.Systemic alterations in iron homeostasis,hepcidin levels and anemia were not detected in colitic rats.Iron deposition in the colon was only observed during colitis.Hepcidin gene expression was increased in HT-29 cells after IL-6 and lipopolysaccharide[a toll-like receptor 4(TLR-4)ligand]treatment.Deferoxamine,ferric citrate and peptidoglycan(a TLR-2 ligand)were unable to alter the in vitro expression of hepcidin in HT-29 cells.CONCLUSION:Colitis increased local hepcidin-25 expression,which was associated with the IL-6/Stat-3 signaling pathway.An increase in local iron sequestration was also observed,but additional studies are needed to determine whether this sequestration is a defensive or pathological response to intestinal inflammation. | Erica Martins Ferreira Gotardo Gilberto de Almeida Ribeiro Thayane Rodrigues Leite Clemente Camila Henrique Moscato Renata Bortolin Guerra Tome Thalita Rocha Jose Pedrazzoli Jr Marcelo Lima Ribeiro Alessandra Gambero | 2014 | World Journal of Gastroenterology2014，20，15： | 1 |
| 15 | Migration of diethylhexyl phthalate from PVC bags into intravenous cyclosporine solutions显示文摘 | Gotardo M A | 2005 | Journal of Pharmaceutical and Biomedical Analysis2005，38，： | 1 |
| 16 | Range Image Segmentation Into Planar and Quadric Surfaces Using an Improved Robust Estimator and Genetic Algorithm显示文摘 | Gotardo P Bellon O | 2004 | IEEE Transactions on System Man Cybernetics B2004，34，6： | 1 |
| 17 | Range Image Segmentation Into Planar and Quadric Surfaces Using an Improved Robust Estimator and Genetic Algorithm显示文摘 | Gotardo P Bellon O | 2004 | IEEE Transactions on System Man Cybernetics B2004，34，6： | 1 |
| 18 | Hepatic pathology in Capillaria hepatica infected mice 显示文摘 | Gotardo BM Andrade RG Andrade ZA | 2000 | Rev Soc Bras Med Trop2000，34，4： | 1 |
| 19 | Spectrophotometric Determination of Formaldehyde with Chromotropic Acid in Phosphoric Acid Medium Assisted by Microwave Oven显示文摘 | Gigante A C Gotardo M A Tognolli J O Pezza L Pezza H R | 2004 | Microchemical Journal2004，77，1： | 1 |
| 20 | Liver transplantationand quality of life : relevance of a specific liver disease questionnaire显示文摘 | Gotardo DR Strauss E Teixeira MC | 2008 | Liver Int2008，28，1： | 1 |
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