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| 1 | Luciferase reporter phage phAE85 for rapid detection of rifampicin resistance in clinical isolates of Mycobacterium tuberculosis显示文摘Objective:To evaluate luciferase reporter phage(LRP)phAE85 in rapid detection of rifampicin resistance in a region where TB is endemic.Methods:One hundred and ninety primary isolates on Lowenstein-Jensen medium were tested.Middlebrook 7H9 complete medium with and without rifampicin at 2μg/mL was inoculated with standard inoculum from suspensions of the clinical isolate.After incubation for 72 h,LRP was added.Following 4 h of further incubation,light output from both control and test was measured as relative light units.Strains exhibiting a reduction of less than 50%relative light units in the drug containing vial compared to control were classified as resistant.Results were compared with the conventional minimum inhibitory concentration method(MIC)of drug susceptibility testing.Results:The two methods showed high level of agreement of 97%(CI 0.94,0.99)and P value was 0.000 1.The sensitivity and specificity of LRP assay for detection of rifampicin resistance were 91%h(CI 0.75,0.98)and 99ct(CI0.95,1.00)respectively.Time to detection of resistance by LRP assay was 3 d in comparison with 28 d by the minimum inhibitory concentration method.Conclusions:LRP assay with phAE85 is 99%specific,91%sensitive and is highly reproducible.Thus the assay offers a simple procedure for drug sensitivity testing,within die scope of semi-automation. | Gomathi Sivaramakrishnan Balaji Subramanyam Ponnuraja C Vanaja Kumar | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，9： | 4 |
| 2 | Secondary metabolite credentials ofEvolvulus alsinoides by high performance thin layer chromatography(HPTLC)显示文摘Plants and plant-based products are the bases of many modern pharmaceuticals that are current in use today for various diseases.The aim of the study was to investigate the biochemical constituents and high performance thin layer chromatography(HPTLC) finger printing of the ethanolic extract of Evolvulus alsinoides.Phytochemical screening was done by standard procedures and HPTLC method was also established to analyze alkaloids,flavonoids and phenolic compounds from the ethanolic extract of Evolvulus alsinoides.Preliminary phytochemical screening showed that ethanol extracted more secondary metabolites than other solvents.HPTLC fingerprinting analysis showed the presence of various alkaloids,flavonoids and phenols(quercetin) in the ethanolic extract.It can be concluded that Evolvulus alsinoides may serve as a source of potent antioxidants that may be used in the prevention of various diseases such as cancer,diabetes and cardiovascular diseases due to the presence of phenolic compounds.HPTLC finger print of Evolvulus alsinoides may be useful in the differentiation of the species from adulterants and act as a biochemical marker for this medicinally important plant in the pharmaceutical industry and plant systematic studies. | Duraisamy Gomathi Manokaran Kalaiselvi Ganesan Ravikumar Dominic Sophia Velliyur Kanniappan Gopalakrishnan Chandrasekar Uma | 2012 | The Journal of Biomedical Research2012，26，4： | 2 |
| 3 | Dermal wound healing processes with curcumin incorporated collagen films 显示文摘 | Gopinath D Ahmed M R Gomathi K | 2004 | Biomaterials2004，25，10： | 1 |
| 4 | Beneficial effects of cetyltrimethylammonium bromide in the modification of electrodes with cobalt hexacyanoferrate surface films显示文摘 | Vittal R Gomathi H | 2002 | J Phys Chem B2002，106，10： | 1 |
| 5 | CTAB-Promoted prussian blue-modified electrode and its cation transport characteristics for K^+, Na^+, Li^+, and NH4^+ Ions显示文摘 | Vittal R Kim K J Gomathi H | 2008 | J Phys Chem B2008，112，4： | 1 |
| 6 | Dermal Wound Healing Processes with Curcumin Incorporated Collagen Films显示文摘 | Gopinath D Rafiuddin Ahmed M Gomathi K | 2004 | Biomaterials2004，25，10： | 1 |
| 7 | Der- mal wound healing proeesseswith curcumin incorporated collagen films显示文摘 | Gopinath D Rafiuddin Ahmed M Gomathi K | 2004 | Biomaterials2004，25，10： | 1 |
| 8 | Prophylactic antibiotics in open mesh repair of inguinal hernia:a randomized controlled trial 显示文摘 | GOMATHI SHANKAR V SRINIVASAN K SARATH C | 2010 | Int J Surg2010，8，6： | 1 |
| 9 | MoS2 and WS2 analogues of graphene 显示文摘 | Ramakfishina M H Gomathi A Manna A K | 2010 | Angewandte Chemie2010，122，24： | 1 |
| 10 | Electrosynthesis of potassium permanganate in a cation exchange membrane cell显示文摘 | Subramanian K Gomathi K G Asokan K | 2008 | Industrial and Engineeri ng Chemistry Research2008，47，22： | 1 |
| 11 | Polygalacturonase-inhibiting proteins in plant defense显示文摘 | Gomathi V Gnanamanickam S S | 2004 | Current Science2004，87，9： | 1 |
| 12 | Reliability of Mycobacterial Growth Indicator Tube (MGIT) 960 for the detection of isoniazid resistance in a tuber- culosis endemic setting显示文摘 | Gomathi NS Kumar V | 2014 | Indian J Med Res2014，139，3： | 1 |
| 13 | Derivatised nick- el and cobalt oxide modified electrodes : effect of surfactant 显示文摘 | Vittal R Gomathi H Prabhakara Rao G | 2001 | Journal of Electroanalytical Chemistry2001，497，12： | 1 |
| 14 | Homocysteine levels in the vitreous of proliferative diabetic retinopathy and rhegmatogenous retinal detachment: its modulating role on lysyl oxidase 显示文摘 | Coral K Angayarkanni N Gomathy N | 2009 | Invest Ophthalmol Vis Sci2009，50，8： | 1 |
| 15 | Surface modification ofpolypropylene using argon plasma:Statistical optimization of the process variables显示文摘 | Gomathi N Neogi S | | 0，，17： | 1 |
| 16 | Characterization of Mo doped TiO2 and its enhanced photocatalytic activity under visible light显示文摘 | L Gomathi Devi B Narasimha Murthy | 2008 | Catalysis letters2008，125，34： | 1 |
| 17 | MoS2 and WS2 analogues of graphene显示文摘 | Matte H Gomathi A Manna A K | | 0，，24： | 1 |
| 18 | Dermal woundhealing processes with curcumin incorporated collagen films显示文摘 | Gopinath D Ahmed M R Gomathi K | 2004 | Biomaterials2004，25，10： | 1 |
| 19 | A protease from the medicinal mushroom Pleurotus sajor-caju; production, purification and partial characterization显示文摘 | Ravikumar G Gomathi D Kalaiselvi M | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，1： | 1 |
| 20 | Cold dissolution method for the determination of uranium in various geological materials at trace levels by laser fluorometry显示文摘 | Gomathy A B Umashankar V | 1997 | Talanta1997，44，： | 1 |
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