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| 1 | APOPTOSIS AND TAXOL PRODUCTION IN SUSPENSION CULTURES OF Taxus spp.CELLS显示文摘The hallmark of apoptosis, in suspension cultures of Taxus spp. cells induced by fungal extractive or by abiotic means, was studied by total DNA agarose gel electrophoresis and in situ end-labeling. The cleavage of nuclear DNA (nDNA) into oligonucleosomal fragments(DNA laddering) was a characteristic of apoptosis, which involved cell shrinkage, condensation of cytoplasm and tracheary elements differentiation. Terminal deoxynucleotidy transferase-mediated dUTP nick end in situ labeling (TUNEL) assay of Taxus spp. cells showed that fungal extractive or abiotic elicltors (Ce4+, Taxol, H2O2) induced TUNEL positive. Also, the increase of the apoptotic cell ratio was accompanied by the increase of secondary metabolites (especially Taxol). These results suggest that apoptosis may have some coincidence with biosynthesis of Taxol. The implication of apoptosis for the production of secondary metabolites in plant cell cultures is discussed. | Yuan Y. J., Ma L Y., Ge Z. Q.,and Miao Z. Q. Zeng A.P. (Department of biochemical engineering, Tianjin University, Tianjin 300072, China) (Biochemical Engineering Division, GBF,D-38124 Breunschweig, Germany) | 2000 | 化工学报2000，51，S1： | 2 |
| 2 | Trends in prevalence and characteristics of post-neonatal cerebral pasly cases: An European registry-based study显示文摘 | Germany L Ehlinger V Klapouszczak D | 2013 | Res Dev Disabil2013，34，5： | 1 |
| 3 | Performance comparison of different element shapes used in printed reflectarrays显示文摘 | BOZZI M GERMANI S PERREGRINI L | 2003 | IEEE Antennas and Wireless Propagation Letters2003，2，1： | 1 |
| 4 | Universal Definition of Myocardial Infarction显示文摘 | Kristian Thygesen Joseph S. Alpert Harvey D. White Kristian Thygesen (Denmark) Joseph S. Alpert (USA) Harvey D. White (New Zealand) Allan S. Jaffe Fred S. Apple (USA) Marcello Galvani (Italy) Hugo A. Katus (Germany) L Kristin Newby (USA) Jan Ravkilde (Den | 2007 | Circulation2007，，22： | 1 |
| 5 | Universal Definition of Myocardial Infarction显示文摘 | Kristian Thygesen Joseph S. Alpert Harvey D. White Kristian Thygesen (Denmark) Joseph S. Alpert (USA) Harvey D. White (New Zealand) Allan S. Jaffe Fred S. Apple (USA) Marcello Galvani (Italy) Hugo A. Katus (Germany) L Kristin Newby (USA) Jan Ravkilde (Den | 2007 | Circulation2007，，22： | 1 |
| 6 | Enantioselective Epoxidations of Alkenes Catalyzed by ( Salen ) Mn ( Ⅲ ) in Aqueous Surfactant Systems显示文摘 | Ballistreri F P Brinchi L Germani R | 2008 | Tetrahedron2008，4，10： | 1 |
| 7 | Diagnostic value of transtho- racic echocardiography in differentiating transient left ven- tricular apical ballooning from anterior myocardial infarction 显示文摘 | KLINIKEN S GERMANY L | 2006 | Circulation2006，114，： | 1 |
| 8 | Histological subclassification of cirrhosis显示文摘 | Germani G Dhillon A Andreana L | 2010 | Hepatology2010，52，2： | 1 |
| 9 | Universal Definition of Myocardial Infarction显示文摘 | Kristian Thygesen Joseph S. Alpert Harvey D. White Kristian Thygesen (Denmark) Joseph S. Alpert (USA) Harvey D. White (New Zealand) Allan S. Jaffe Fred S. Apple (USA) Marcello Galvani (Italy) Hugo A. Katus (Germany) L Kristin Newby (USA) Jan Ravkilde (Den | 2007 | Circulation2007，，22： | 1 |
| 10 | IGF-I and IGF-binding protein-1 are related to cortisol in human cord blood显示文摘 | Cianfarani S Germani D Rossi L | 1998 | Eur Endocrinol1998，138，： | 1 |
| 11 | Evidence-based diagnosis and locoregional therapy for hepatocellular carcinoma显示文摘 | Pleguezuelo M Germani G Marelli L | 2008 | Expert Rev Gastroenterol Hepatol2008，2，6： | 1 |
| 12 | Trends in prevalence and characteristics of post-neonatal cerebral palsy cases : A European registry-based study 显示文摘 | Germany L Ehlinger V Klapouszczak D | 2013 | Res Dev Disabil2013，34，5： | 1 |
| 13 | A high-speed oscillator-based truly random number source for cryptographic applications on a smart card IC显示文摘 | Bucci M Germani L Luzzi R | 2003 | Computers IEEE Transactions on2003，52，4： | 1 |
| 14 | Trends in prevalence and characteristics of post-neonatal cerebral palsy cases:a European registry-based study显示文摘 | Germany L Ehlinger V Klapouszczak D | 2013 | Res Dev Disabil2013，34，5： | 1 |
| 15 | A heigh- speed oscillator-based truly random number source for cryptographic applacations on a smart card IC显示文摘 | BUCCI M GERMANI L LUZZI R | 2003 | IEEE Trans Computers2003，52，4： | 1 |
| 16 | Bladder malformations in a model of myelomeningocele显示文摘 | Encinas JL Germani M Burgos L | 2010 | Cir Pediatr2010，23，2： | 1 |
| 17 | Trends in prevalence and characteristics of post-neonatal cerebral palsy cases : A European registry-based study 显示文摘 | Germany L Ehlinger V Klapouszczak D | 2013 | Res Dev Disabil2013，34，5： | 1 |
| 18 | Diagnostic value of transthoracic echocardiography in differentiating transient left ventricular apical ballooning from anterior myocardial infarction显示文摘 | Kliniken S Germany L | 2006 | Circulation2006，114，： | 1 |
| 19 | Assessmant of coronary heart disease risk among viscose rayon workers exposed to carbon disulfide at concentrations of about 30 rag/m^3 显示文摘 | FRANCO G MALAMANT T GERMANI L ct al | 1982 | Scand Work Environ Health1982，8，2： | 1 |
| 20 | Universal Definition of Myocardial Infarction显示文摘 | Kristian Thygesen Joseph S. Alpert Harvey D. White Kristian Thygesen (Denmark) Joseph S. Alpert (USA) Harvey D. White (New Zealand) Allan S. Jaffe Fred S. Apple (USA) Marcello Galvani (Italy) Hugo A. Katus (Germany) L Kristin Newby (USA) Jan Ravkilde (Den | 2007 | Circulation2007，，22： | 1 |
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