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| 1 | Effects of the activities of key enzymes involved in starch biosynthesis on the fine structure of amylopectin in developing rice (Oryza sativa L.) endosperms显示文摘The dynamic changes of the activities of enzymes involving in starch biosynthesis, including ADP-glucose pyrophosphorylase (AGPase), soluble starch synthases (SSS), starch branching enzyme (SBE) and starch debranching enzymes (DBE) were studied, and changes of fine structure of amy- lopectin were characterized by isoamylase treatment during rice grain development, using trans anti-waxy gene rice plants. The relationships between the activities of those key enzymes were also analyzed. The amylose synthesis was significantly inhibited in transgenic Wanjing 9522, but the total starch content and final grain weight were less affected as compared with those of non-transgenic Wanjing 9522 rice cultivar. Analyses on the changes of activities of enzymes involving in starch bio- synthesis showed that different enzyme activities were expressed differently during rice endosperm development. Soluble starch synthase is relatively highly expressed in earlier stage of endosperm de- velopment, whilst maximal expression of granule-bound starch synthase (GBSS) occurred in mid-stage of endosperm development. No obvious differences in changes of the activities of AGPase and SBE between two rice cultivars investigated, except the DBEs. Distribution patterns of branches of amy- lopectin changed continually during the development of rice grains and varied between two rice culti- vars. It was suggested that amylopectin synthesis be prior to the synthesis of amylose and different enzymes have different roles in controlling syntheses of branches of amylopectin. | Lü Bing, GUO ZhiGang & LIANG JianSheng College of Bioscience and Biotechnology, Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou Univer- sity, Yangzhou 225009, China | 2008 | Science China(Life Sciences)2008，51，10： | 2 |
| 2 | Impact of copy number of distinct SV40PolyA segments on expression of a GFP reporter gene显示文摘The presence of Alu repeats downregulates the expression of the green fluorescent protein(GFP) gene.We found that SV40PolyA(PolyA,240 bp),in either orientation,eliminated the inhibition of GFP gene expression induced by Alu repeats when it was placed between the GFP gene and the Alu repeats.In this study,4 different segments(each 60 bp) were amplified from antisense PolyA(PolyAas) by PCR,and inserted upstream of Alu14 in pAlu14 plasmid(14 Alu repeats inserted downstream of the GFP gene in vector pEGFP-C1 in a head-tail tandem manner).Segments 1F1R(the first 60 bp segment at the 5' end of PolyAas) and 4F4R(the fourth 60 bp segment from the 5' end of PolyAas) did not activate GFP gene expression,whereas 2F2R and 3F3R(the middle two segments) did(as detected by Northern blot analysis and fluorescent microscopy).Different copy numbers of 2F2R and 3F3R segments,in a head and tail tandem manner,were inserted downstream of the GFP gene in pAlu14.p2F2R*4-Alu28,p3F3R*4-Alu18 and p3F3R*4-Alu28 were used as length controls to verify that the decrease in the expression of GFP was not due to the increased length of the inserted segment in the expression vectors.We found that 2 and 4 copies of 2F2R or 3F3R activated the GFP gene more strongly than one copy of them.However,more than 8 copies of 2F2R or 3F3R reduced the activation of the GFP gene.We concluded that SV40PolyAas contained at least two gene-activating elements(2F2R and 3F3R) and 2-4 copies of 2F2R or 3F3R were optimal for the expression of the GFP gene. | YIN Kang,WANG XiuFang,MA Huan,XIE Ying,FENG JingJing,YANG QinQing & Lü ZhanJun Hebei Key Lab of Laboratory Animal,Department of Genetics,Hebei Medical University,Shijiazhuang 050017,China | 2010 | Science China(Life Sciences)2010，53，5： | 2 |
| 3 | Engineering ecological protection against landslides in diverse mountain forests: choosing cohesion models显示文摘 | MAO Z SAINT-ANDRE L GENET M | | 0，，： | 1 |
| 4 | Aspects of multidrug resistance 显示文摘 | Biedler J L Genetic P C | 1992 | Cancer1992，70，16： | 1 |
| 5 | Calcium carbonate, but not sevelamer, is associated with better outcomes in hemodialysis patients: results from the French ARNOS study 显示文摘 | Jean G Lataillade D Genet L | 2011 | Hemodial Int2011，15，： | 1 |
| 6 | Phenotypic and muscle fiber characteristics in relation to growth, carcass, and meat quality traits in large white pigs显示文摘 | C Larzul L Lefaueheur P genetic parameters for longissimus Ecolan | 1997 | Journal of Animal Science1997，75，12： | 1 |
| 7 | Association between very low PTH levels and poor survival rates in haemodialysis patients:results from the French ARNOS cohort显示文摘 | Jean G Lataillade D Genet L | | 0，，： | 1 |
| 8 | coli显示文摘 | Viveiros M Dupont M Rodrigues L et aL Antibiotic stress genetic response and altered permeability ofE | 2007 | PLoS One2007，2，4： | 1 |
| 9 | Evaluation and comparison of in-vitro dissolution profiles for different brands of amoxicillin capsules 显示文摘 | Kassaye L Genete G | 2013 | Afr Health Sci2013，13，2： | 1 |
| 10 | linkage map in man using restriction fragment length polymorphisms显示文摘 | BOSTEIN D WIITE R Construction of a genetic L SKOLNICK M | 1980 | Am J Hum Genet1980，32，3： | 1 |
| 11 | Thrombotic complications of implanted central venous access devices显示文摘 | Labourey J L Lacroix P Genet D | 2004 | Bull Cancer2004，91，5： | 1 |
| 12 | The high prevalence of malnutrition in elderly diabetic patients: implications for anti-diabetic drug treatments显示文摘 | Vischer UM Perrenoud L Genet C | 2010 | Diabet Med2010，27，8： | 1 |
| 13 | Biological mode of action of Famoxadone on Plasmopara viticola and Phytophthora infestans 显示文摘 | Andrieu N Jaworska G Genet J L | 2001 | Crop Protetion2001，20，： | 1 |
| 14 | Guidelines for the diagnosis and management of Marfan syndrome 显示文摘 | Ades L CSANZ Cardiovascular Genetics Working Group | 2007 | Heart Lung and Circulation2007，16，1： | 1 |
| 15 | Grain yield and yield components显示文摘 | Veldboom L R Lee-M Genetic mapping of quantitative trait loci in maize in stress and nonstress environments: I | 1996 | Crop Science1996，36，13： | 1 |
| 16 | evidence of a complementary epistasis between two QILs显示文摘 | Pressoir G Albar L Ahmadi N Genetic basis and mapping of the resistance to rice yellow mottle virus Ⅱ | 1998 | Theor Appl Genet1998，97，： | 1 |
| 17 | and monoecious Pistacia atlantica Desf 显示文摘 | Yusa Turkeli Salih Kafkas First genetic linkage map in pistachio constructed using an interspecific cross between Pistacia vera L | 2013 | Scientia Horticulturae2013，151，： | 1 |
| 18 | Effect of dose rate and mixtures of fungicides on selection for QoI resistance in populations of Plasmopara viticola 显示文摘 | Genet J L Jaworska G Deparis F | 2006 | Pest Management Science2006，62，： | 1 |
| 19 | The FAS ligand promoter poly- sm, rs763110 (-844C>T), contributes to cancer suscep- evidence from 19 case-control studies显示文摘 | Zhang morph tibility: Genet Z Qiu L Wang M | 2009 | Eur J Hum2009，17，10： | 1 |
| 20 | Triglyceride-mediated pathways and coronary disease:collaborative analy-sis of 101studies显示文摘 | Triglyceride Coronary Disease Genetics Consortium and Emer-ging Risk Factors Collaboration Sarwar N Sandhu M S Rick-etts S L Butterworth A S Di Angelantonio E | 2010 | Lancet2010，375，： | 1 |
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