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| 1 | Prunus genetics and applications after de novo genome sequencing:achievements and prospects显示文摘Prior to the availability of whole-genome sequences,our understanding of the structural and functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping of important traits and development of EST resources.With public release of the peach genome and others that followed,significant advances in our knowledge of Prunus genomes and the genetic underpinnings of important traits ensued.In this review,we highlight key achievements in Prunus genetics and breeding driven by the availability of these whole-genome sequences.Within the structural and evolutionary contexts,we summarize:(1)the current status of Prunus whole-genome sequences;(2)preliminary and ongoing work on the sequence structure and diversity of the genomes;(3)the analyses of Prunus genome evolution driven by natural and man-made selection;and(4)provide insight into haploblocking genomes as a means to define genome-scale patterns of evolution that can be leveraged for trait selection in pedigree-based Prunus tree breeding programs worldwide.Functionally,we summarize recent and ongoing work that leverages whole-genome sequences to identify and characterize genes controlling 22 agronomically important Prunus traits.These include phenology,fruit quality,allergens,disease resistance,tree architecture,and self-incompatibility.Translationally,we explore the application of sequence-based marker-assisted breeding technologies and other sequence-guided biotechnological approaches for Prunus crop improvement.Finally,we present the current status of publically available Prunus genomics and genetics data housed mainly in the Genome Database for Rosaceae(GDR)and its updated functionalities for future bioinformatics-based Prunus genetics and genomics inquiry. | Maria JoséAranzana Véronique Decroocq Elisabeth Dirlewanger Iban Eduardo Zhong Shan Gao Ksenija Gasic Amy Iezzoni Sook Jung Cameron Peace Humberto Prieto Ryutaro Tao Ignazio Verde Albert G.Abbott Pere Arús | 2019 | Horticulture Research2019,6,1: | 8 |
| 2 | RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction显示文摘 | Ksenija Gasic Alvaro Hernandez Schuyler S. Korban | 2004 | Plant Molecular Biology Reporter2004,,4: | 2 |
| 3 | RosBREED:bridging the chasm between discovery and application to enable DNA-informed breeding in rosaceous crops显示文摘The Rosaceae crop family(including almond,apple,apricot,blackberry,peach,pear,plum,raspberry,rose,strawberry,sweet cherry,and sour cherry)provides vital contributions to human well-being and is economically significant across the U.S.In 2003,industry stakeholder initiatives prioritized the utilization of genomics,genetics,and breeding to develop new cultivars exhibiting both disease resistance and superior horticultural quality.However,rosaceous crop breeders lacked certain knowledge and tools to fully implement DNA-informed breeding—a“chasm”existed between existing genomics and genetic information and the application of this knowledge in breeding.The RosBREED project(“Ros”signifying a Rosaceae genomics,genetics,and breeding community initiative,and“BREED”,indicating the core focus on breeding programs),addressed this challenge through a comprehensive and coordinated 10-year effort funded by the USDA-NIFA Specialty Crop Research Initiative.RosBREED was designed to enable the routine application of modern genomics and genetics technologies in U.S.rosaceous crop breeding programs,thereby enhancing their efficiency and effectiveness in delivering cultivars with producer-required disease resistances and market-essential horticultural quality.This review presents a synopsis of the approach,deliverables,and impacts of RosBREED,highlighting synergistic global collaborations and future needs.Enabling technologies and tools developed are described,including genome-wide scanning platforms and DNA diagnostic tests.Examples of DNA-informed breeding use by project participants are presented for all breeding stages,including pre-breeding for disease resistance,parental and seedling selection,and elite selection advancement.The chasm is now bridged,accelerating rosaceous crop genetic improvement. | Amy F.Iezzoni Jim McFerson James Luby Ksenija Gasic Vance Whitaker Nahla Bassil Chengyan Yue Karina Gallardo Vicki McCracken Michael Coe Craig Hardner Jason D.Zurn Stan Hokanson Eric van de Weg Sook Jung Dorrie Main Cassia da Silva Linge Stijn Vanderzande Thomas M.Davis Lise L.Mahoney Chad Finn Cameron Peace | 2020 | Horticulture Research2020,7,1: | 2 |
| 4 | Throm- bospondin, a potentiator of tumor cell metastasis 显示文摘 | Tuszynski GP Gasic TB Rothman VL | 1987 | Cancer Res1987,47,: | 1 |
| 5 | Adaptability to drought in sugar beet cultivars显示文摘 | Stajner D Mimica-Dukic N Gasic O | 1995 | Biologia Plantarum1995,37,: | 1 |
| 6 | RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction 显示文摘 | Gasic K Hemandez A Korban S S | 2004 | Plant Mol Biol Rep2004,22,: | 1 |
| 7 | To die or to sleep,perhaps to dream显示文摘 | Gasic GP Nicotera P | 2003 | Toxicol Lett2003,139,23: | 1 |
| 8 | Transgenie rose lines harboring an antimierobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa ) 显示文摘 | LI X Q GASIC K CAMMUE B | 2003 | Planta2003,218,: | 1 |
| 9 | The amino acid sequence of antistasin:a potent inhibitor of factor Xa reveals a repeated internal structure显示文摘 | Nutt E Gasic T Rodkey J | 1988 | J Biol Chem1988,263,10: | 1 |
| 10 | Protocol:a highly sensitive RT-PCR method for detection and quantification of microRNAs显示文摘 | Varkonyi Gasic E Wu R Wood M | 2007 | Plant Methods2007,3,: | 1 |
| 11 | Structure and distribution of the Notch protein in developing Drosophila显示文摘 | Kidd S Baylies MK Gasic GP | 1989 | Genes Dev1989,3,8: | 1 |
| 12 | To die or to sleep, perhaps to dream 显示文摘 | Gasic GP Nicotera P | 2003 | Toxicol Lett2003,139,23: | 1 |
| 13 | Comparative analysis and functional annotation of a large expressed sequence tag collection of apple 显示文摘 | Gasic K Gonzalez D O Thimmapuram J | 2009 | Plant Genome2009,2,1: | 1 |
| 14 | Gly-Gln: Metabolism and effects on organ balances of amino acids in postabsorptive and starved subjects 显示文摘 | Locs H Hubl W Gasic S etal | 1992 | Am J Physiol1992,,262: | 1 |
| 15 | Development of a set of SNP markers present in expressed genes of the apple 显示文摘 | Chagne D Gasic K Crowhurst RN | 2008 | Genomics2008,92,5: | 1 |
| 16 | RNA extraction from different apple tissues rich in polyphenos and polysaccharides for cDNA library construction显示文摘 | Gasic K Hernandez A Korban S S | 2004 | Plant Molecular Biology Reporter2004,22,: | 1 |
| 17 | Alterations in angiotensin release and vascular reactivity in hypertensive men; a pilot study显示文摘 | Gasic S Ratheiser K Wagner O | 1999 | Am J Hypertension1999,12,11: | 1 |
| 18 | Molecular analysis of the c-myc locus in normal tissue and in avian leukosis virus-induced lymphomas显示文摘 | Neel BG Gasic GP Rogler CE | | 0,,01: | 1 |
| 19 | Transgenic Indian mustard (Brassica juncea) plants expressing an Arabidopsis phytochdatin synthase (AtPCS1) exhibit enhanced As and Cd tolerance显示文摘 | Gasic K Korban S S | | 0,,04: | 1 |
| 20 | Characteristics and trans- ferability of new apple EST--derived SSRs to other Rosaceae species 显示文摘 | Gasic K Han Y P Kertbundit S | 2009 | Mol Breeding2009,23,: | 1 |