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9篇 您的检索式：作者名="GangHan"

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		题名	作者	年代	出处	被引量
	1	Expression of TIMP-3 Gene by Construction of a Eukaryotic Cell Expression Vector and Its Role in Reduction of Metastasis in a Human Breast Cancer Cell Line显示文摘The present study is aimed at studying the gene for TIMP-3, a mammalian tissue inhibitor, by constructing a recombinant eukaryotic cell vector for gene therapy in human breast cancer. We obtained the TIMP-3 gene from the human placent by RT-PCR. TIMP-3 gene was subcloned into pcDNA3.1 vetor from pMD18T vector by means of gene cloning to construct pcDNA3.1 recombinant vector. Human breast cancer cell line MDA-MB-453 was transfected with pcDNA3.1-TIMP3 recombinant vector using lipofectamine reagent. Then the expression of TIMP-3 and the effect on the metastasis of MDA-MB-453 were examined. The correct construction of pcDNA-TIMP3 was identified by means of restriction enzyme analysis, PCR amplication and nucleotide sequencing. Western blotting showed that the transfected cells were able to express TIMP-3,indicating that our construction of the pcDNA-TIMP3 eukaryotic expression vector was constructed successfully. Our experiments further indicated that the potential of metastasis was significantly reduced for the transfected cell line MDA-MB-453.	XichunHan HongZhang MingkuJia GangHan WeidongJiang	2004	Cellular & Molecular Immunology2004，1，4：	11
	2	National science training policy and early scientific careers in france and the united states 显示文摘	Monica Ganghan Stephane Robin	2004	Research Policy2004，，33：	1
	3	Light‐Regulated Release of DNA and Its Delivery to Nuclei by Means of Photolabile Gold Nanoparticles显示文摘	GangHan Chang‐ChengYou Byoung‐jinKim Rosemary S.Turingan Neil S.Forbes Craig T.Martin Vincent M.Rotello	2006	Angewandte Chemie International Edition2006，，19：	1
	4	National science training policy and early scientific careers in France and the United States 显示文摘	Ganghan M Robin S	2004	Research Policy2004，33，4：	1
	5	Effects of crystalloidcolloid solutions on traumatic brain injury显示文摘	Elliott M B Jallo J J Ganghan J P	2007	J Neurotrauma2007，24，1：	1
	6	Using curriculum vitae to compare some impacts of NSF research grants with research center fundlng显示文摘	Ganghan M Bozeman B	2002	Research Evaluation2002，11，1：	1
	7	Using curriculum vitae to compare some impacts of NSF research grants with research center funding 显示文摘	Ganghan M Bozeman B	2002	Research Evaluation2002，11，1：	1
	8	Impacts of grants and contracts on academic researchers' interactions with industry显示文摘	Bozeman B Ganghan M	2007	Research Policy2007，，05：	1
	9	Dysphagia,hoarseness, and unilateral true vocal fold motion impairment following anterior cervical disectomy and fusion 显示文摘	Baron EM Soliman AM Ganghan JP	2003	Ann Otol Rhinol Laryngo12003，112，1：	1

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