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| 1 | Pericyte-like differentiation of human adipose-derived mesenchymal stem cells:An in vitro study显示文摘BACKGROUND Adipose-derived mesenchymal stem cells(ASCs)are characterized by long-term self-renewal and a high proliferation rate.Under adequate conditions,they may differentiate into cells belonging to mesodermal,endodermal or ectodermal lineages.Pericytes support endothelial cells and play an important role in stabilizing the vessel wall at the microcirculation level.The loss of pericytes,as occurs in diabetic retinopathy,results in a breakdown of the blood-retina barrier(BRB)and infiltration of inflammatory cells.In this context,the use of pericytelike differentiated ASCs may represent a valuable therapeutic strategy for restoring BRB damage.AIM To test in vitro strategies to obtain pericyte-like differentiation of human ASCs(hASCs).METHODS Different culture conditions were tested:hASCs cultured in a basal medium supplemented with transforming growth factorβ1;and hASCs cultured in a specific pericyte medium(PM-hASCs).In a further sample,pericyte growth supplement was omitted from the PM.In addition,cultures of human retinal pericytes(hRPCs)were used for comparison.Pericyte-like differentiation of hASCs was tested by immunocytochemical staining and western blotting to evaluate the expression ofα-smooth muscle actin(α-SMA)and neural/glial antigen 2(NG2).Interactions between human retinal endothelial cells(hRECs)and different groups of hASCs were investigated in co-culture experiments.In these cases,the expression of typical junctional proteins such as vascular endothelial-Cadherin,zonula occludens-1 and Occludin were assessed in hRECs.In an in vitro model of the BRB,values of trans-endothelial electrical resistance were measured when hRECs were co-cultured with various groups of pretreated hASCs.The values observed were compared with co-cultures of hRECs and hRPCs as well as with cultures of hRECs alone.Three-dimensional co-cultures of hRECs and hRPCs or pericyte-like hASCs in Matrigel were designed to assess their reciprocal localization.RESULTS After 3-6 d of culture,α-SMA and NG2 immunocytochemistry showed that the closest pericyte-like phenotype was observed when hASCs were cultured in Pericyte Medium(PM-hASCs).In particular,α-SMA immunoreactivity,already visible at the basal level in pericytes and ASCs,was strongly increased only when transforming growth factor was added to the culture medium.NG2 expression,almost undetectable in most conditions,was substantially increased only in PMhASCs.Immunocytochemical results were confirmed by western blot analysis.The presence of pericyte growth supplement seems to increase NG2 expression rather thanα-SMA,in agreement with its role in maintaining pericytes in the proliferative state.In co-culture experiments,immunoreactivity of vascular endothelial-Cadherin,zonula occludens-1 and Occludin was considerably increased in hRECs when hRPCs or PM-hASCs were also present.Supporting results were found by trans-endothelial electrical resistance measurements,gathered at 3 and 6 d of co-culture.The highest resistance values were obtained when hRECs were co-cultured with hRPCs or PM-hASCs.The pericyte-like phenotype of PM-hASCs was also confirmed in three-dimensional co-cultures in Matrigel,where PM-hASCs and hRPCs similarly localized around the tubular formations made by hRECs.CONCLUSION PM-hASCs seem able to strengthen the intercellular junctions between hRECs,likely reinforcing the BRB;thus,hASC-based therapeutic approaches may be developed to restore the integrity of retinal microcirculation. | Giuliana Mannino Florinda Gennuso Giovanni Giurdanella Federica Conti Filippo Drago Salvatore Salomone Debora Lo Furno Claudio Bucolo Rosario Giuffrida | 2020 | World Journal of Stem Cells2020，12，10： | 3 |
| 2 | Potential therapeutic applications of mesenchymal stem cells for the treatment of eye diseases显示文摘Stem cell-based treatments have been extensively explored in the last few decades to develop therapeutic strategies aimed at providing effective alternatives for those human pathologies in which surgical or pharmacological therapies produce limited effects.Among stem cells of different sources,mesenchymal stem cells(MSCs)offer several advantages,such as the absence of ethical concerns,easy harvesting,low immunogenicity and reduced tumorigenesis risks.Other than a multipotent differentiation ability,MSCs can release extracellular vesicles conveying proteins,mRNA and microRNA.Thanks to these properties,new therapeutic approaches have been designed for the treatment of various pathologies,including ocular diseases.In this review,the use of different MSCs and different administration strategies are described for the treatment of diabetic retinopathy,glaucoma,and retinitis pigmentosa.In a large number of investigations,positive results have been obtained by in vitro experiments and by MSC administration in animal models.Most authors agree that beneficial effects are likely related to MSC paracrine activity.Based on these considerations,many clinical trials have already been carried out.Overall,although some adverse effects have been described,promising outcomes are reported.It can be assumed that in the near future,safer and more effective protocols will be developed for more numerous clinical applications to improve the quality of life of patients affected by eye diseases. | Giuliana Mannino Cristina Russo Anna Longo Carmelina Daniela Anfuso Gabriella Lupo Debora Lo Furno Rosario Giuffrida Giovanni Giurdanella | 2021 | World Journal of Stem Cells2021，13，6： | 2 |
| 3 | Mesenchymal stem cells from adipose tissue which have been differentiated into chondrocytes in three-dimensional culture express lubricin显示文摘 | Musumeci G Lo Furno D Loreto C | | 0，，： | 1 |
| 4 | Pregnancy -related hip diseases: incidence and diagnoses 显示文摘 | STEIB FURNO S LUC M PHAM T | 2007 | Joint Bone Spine2007，74，4： | 1 |
| 5 | Highly Efficient White Organic Light-Emitting Diodes Based on Fluores- cent Blue Emitters 显示文摘 | LEO K ROSENOW T C FURNO M | 2010 | Appl Phys2010，108，11： | 1 |
| 6 | A fast no search fractal image coding method显示文摘 | Furno S Hasegewa O | 2004 | Signal Processing:Image Communication2004，19，5： | 1 |
| 7 | Silver nanoparticles and polymeric medical devices:a new approach to prevention of infection显示文摘 | Furno F Morley KS Wong B | 2004 | J Antimicrob Chemother2004，54，6： | 1 |
| 8 | Monitoring of envi- ronmental pollutants by bioluminescent bacteria 显示文摘 | Girotti S Ferri E N Furno M G | 2008 | Ana- lytica Chimica Acta2008，608，1： | 1 |
| 9 | Silver nanoparticles and polymeric medical devices:a new approach to prevention of infection显示文摘 | Furno F Morley K S Wong B | 2004 | J Antimicrob Chemother2004，54，6： | 1 |
| 10 | Arc-jet testing on Hffi and HfC-based ultra-high temperature ceramic materials显示文摘 | Savino R Furno M D S Silvestroni L | 2008 | J Eur Ceram Soc2008，28，9： | 1 |
| 11 | A simple and effective method to improve intrasplenic rat hepatocyte transplantation 显示文摘 | Sigot V Mediavilla MG Furno G | 2004 | Cell Transplant2004，13，78： | 1 |
| 12 | A simple and effective method to improve intrasplenic rat hepatocyte transplantation 显示文摘 | SIGOT V MEDIAVILLA MG FURNO G | 2004 | Cell Transplant2004，13，： | 1 |
| 13 | Silver nanoparticles and polymericmedical devices:a new approach to prevention of infection?显示文摘 | Furno F Morley KS Wong B | 2004 | JAntimicrob Chemother2004，54，6： | 1 |
| 14 | Curren-t Driven Ro-tating-Kink Mode in a Plasma Column with a Non-Line-TiedFree End显示文摘 | Furno I Intrator T P Ryutov D D | 2006 | Phys Rev Lett2006，97，1： | 1 |
| 15 | Silver Nanopartieles Andpolymerie Medical Devices : a Newapproaehto Prevention-of Infection显示文摘 | FURNO F MORLEY K S WONG B | 2004 | J Antimicrob Chemother2004，54，： | 1 |
| 16 | Silver nanoparticles and polymeric medical devices: a new approach to prevention of infection 显示文摘 | Furno F Morley K S Wong B | 2004 | J Antimicrob Chemother2004，54，6： | 1 |
| 17 | A fuzzy cognitive situ atio naw areness for airp o rt security 显示文摘 | Furno D Loia V Veniero M | 2010 | C ontrol and C ybernetics2010，39，4： | 1 |
| 18 | The effect of Mn/Ti surface treatment on voltage-holdoff performance of alumina insulators in vacuum显示文摘 | MILLER H C FURNO E J | 1978 | J Appl Phys1978，49，11： | 1 |
| 19 | Mesenchy- rnal stem cells from adipose tissue which have been differentiated into chondrocytes in three-dimensional culture express lubricin 显示文摘 | MUSUMECI G FURNO D L LORETO C | 2011 | Experimental Biology and Medicine2011，236，11： | 1 |
| 20 | Parameter instability in quantile regressions显示文摘 | Furno M | 2007 | Statistical Modelling2007，7，4： | 1 |
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