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| 1 | High miR-196a levels promote the oncogenic phenotype of colorectal cancer cells显示文摘AIM: To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis. METHODS: The impact of miR-196a on the restriction targets HoxA7, HoxB8, HoxC8 and HoxD8 was analyzed by reverse transcription polymerase chain reaction (RT-PCR) after transient transfection of SW480 cancer cells. The miR-196a transcription profile in colorectal cancer samples, mucosa samples and diverse cancer cell lines was quantifi ed by RT-PCR. Transiently miR-196a-transfected colorectal cancer cells were used for diverse functional assays in vitro and for a xenograft lung metastasis model in vivo. RESULTS: HoxA7, HoxB8, HoxC8 and HoxD8 were restricted by miR-196a in a dose-dependent and gene-specific manner. High levels of miR-196a activated the AKT signaling pathway as indicated by increased phosphorylation of AKT. In addition, high levels of miR-196a promoted cancer cell detachment,migration, invasion and chemosensitivity towards platin derivatives but did not impact on proliferation or apoptosis. Furthermore, miR-196a increased the development of lung metastases in mice after tail vein injection. CONCLUSION: miR-196a exerts a pro-oncogenic influence in colorectal cancer. | Carl Christoph Schimanski Kirsten Frerichs Fareed Rahman Martin Berger Hauke Lang Peter R Galle Markus Moehler Ines Gockel | 2009 | World Journal of Gastroenterology2009，15，17： | 40 |
| 2 | Coexpression of receptor-tyrosine-kinases in gastric adenocarcinoma-a rationale for a molecular targeting strategy?显示文摘AIM: To define the (co-)expression pattern of target receptor-tyrosine-kinases (RTK) in human gastric adenocarcinoma. METHODS: The (co-)expression pattern of VEGFR1-3,PDGFRα/b and EGFR1 was analyzed by RT-PCR in 51 human gastric adenocarcinomas. In addition,IHC staining was applied for confirmation of expression and analysis of RTK localisation. RESULTS: The majority of samples revealed a VEGFR1 (98%),VEGFR2 (80%),VEGFR3 (67%),PDGFRα (82%) and PDGFRβ(82%) expression,whereas only 62% exhibited an EGFR1 expression. 78% of cancers expressed at least four out of six RTKs. While VEGFR1-3 and PDGFRα revealed a predominantly cytoplasmatic staining in tumor cells,accompanied by an additional nuclear staining for VEGFR3 ,EGFR1 was almost exclusively detected on the membrane of tumor cells. PDGFRβ was restricted to stromal pericytes,which also depicted a PDGFRα expression.receptor-tyrosine-kinases coexpression in gastric adenocarcinoma and might therefore encourage an application of multiple-target RTK-inhibitors within a combination therapy. | Daniel Drescher Markus Moehler Ines Gockel Kirsten Frerichs Annett Müller Friedrich Dünschede Thomas Borschitz Stefan Biesterfeld Martin Holtmann Thomas Wehler Andreas Teufel Kerstin Herzer Thomas Fischer Martin R Berger Theodor Junginger Peter R Galle Carl C Schimanski | 2007 | World Journal of Gastroenterology2007，13，26： | 4 |
| 3 | Laser-Doppler flowmetry in monitoring regulation of rapid microcirculatory changes in spinal cord显示文摘 | Lindersberg PJ Jacobs TP Frerichs KU | 1992 | Am J Physiol1992，263，： | 1 |
| 4 | Significance of abdominal wall CT-angiography in planning DIEA perforator flaps,TRAM flaps and SIEA flaps显示文摘 | Fansa H Schirmer S Frerichs O | | 0，，02： | 1 |
| 5 | A retrospective analysis显示文摘 | Mandracchia VJ Sanders SM Frerichs JA The use of becaplermin (rhPDGF BB) gel for chronic no healing ulcers | 2001 | Clin Podiatr Med Surg2001，18，1： | 1 |
| 6 | Treatment Modality and Vasospasm After Aneurysmal Subarachnoid Hemorrhage显示文摘 | Gross BA Rosalind Lai PM Frerichs KU | | 0，，13： | 1 |
| 7 | Tissue engineering of vascularized bone and soft tissue transplants显示文摘 | Frerich B Kurtz-Hoffmann J Lindemann N | 2000 | Mund Kiefer Gesichtschir2000，4，2： | 1 |
| 8 | Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass,Dicentrarchus labrax显示文摘 | Frerichs G N Rodger H D Peric Z | 1996 | Journal of General Virology1996，77，： | 1 |
| 9 | Electrical impedance tomography(EIT)in applications related to lung and ventilation :a review of experimental and clinical activities显示文摘 | Frerichs I | 2000 | Physiol Meas2000，21，2： | 1 |
| 10 | Bone engineering with adipose tissue derived stromal cells显示文摘 | Hemprich A Frerich B | 2006 | J Craniomaxillofac Surg2006，34，8： | 1 |
| 11 | In vitro culture of embryonic cells from the freshwater prawn,Macrobrachium rosenbergii显示文摘 | Frerichs G N | | 0，，： | 1 |
| 12 | Chronic renal failure is accompanied by endothelial activation and a large increase in microparticle numbers with reduced procoagulant capacity显示文摘 | Trappenburg MC van Schilfgaarde M Frerichs FC | 2012 | Nephrol Dial Transplant2012，27，4： | 1 |
| 13 | Tissue engineering of vascularized bone and soft tissue transplants显示文摘 | Frerich B Kurtz-Hoffmann J Lindemann N | 2000 | Mund Kiefer Gesiehtsehir2000，4，2： | 1 |
| 14 | Temperature,pH and electrolyte sensitivity,and heat,UV and disinfection inactivation of sea bass (Dicentrarchus labrax) neuropathy virus显示文摘 | Frerichs G N Tweedie A Starkey W G | 2000 | Aquaculture2000，185，： | 1 |
| 15 | Improving preanalytic processes using principles of lean production (Toyota production system)显示文摘 | Persoon TJ Zaleski S Frerichs J | | 0，，： | 1 |
| 16 | The safety of dedicated-team catheter-based diagnostic cerebral angiography in the era of advanced noninvasive imaging显示文摘 | Thiex R Norbash AM Frerichs KU | | 0，，2： | 1 |
| 17 | Fibrin sealing and histometrical changes in conventionally sutured microvascular anastomoses显示文摘 | Bschorer R Frerich B Wolburg H | 1993 | J Craniomaxillofic Surg1993，21，5： | 1 |
| 18 | Cerebral sinus and venous thrombosis in rats induces long term deficits in brain function and morphology evidence for a cytotoxic genesis显示文摘 | Frerichs KU Deckert M Kempski O | 1994 | J Cerb Blood Flow Metab1994，14，2： | 1 |
| 19 | Tissue engineering of vascularized bone and soft tissue transplants显示文摘 | Frerich B Kurtz-Hoffmann J Lindemann N | 2000 | Mund Kiefer Gesichtschir2000，42，： | 1 |
| 20 | In vitro model of a vascular stroma for the engineering of vascularized tissues显示文摘 | Frerich B Lindemann N Kurtz-Hoffmann J | | 0，，05： | 1 |
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