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| 1 | 农田生物多样性对昆虫的生态调控作用显示文摘随着农田作物的单一性种植,植食性昆虫暴发、物种流失等问题日益突出,化学农药大量应用于作物有害生物的防治更加剧了对农田生态系统的破坏和不稳定性。因此,从生物多样性-农田生态系统-植食性昆虫的相互关系入手,探讨生物多样性的保护及其在农业有害生物防控上的应用,回归农田生态系统动态平衡的生态调控举措,成为可持续发展农业领域中研究的焦点之一。本文综述了农田生物多样性对昆虫生态影响的重要性及其生态功能,进一步阐述了农田生物多样性在农业生产中的应用及前景。 | 周海波 陈巨莲 程登发 Frederic Francis 刘勇 孙京瑞 | 2012 | 植物保护2012,38,1: | 18 |
| 2 | 昆虫内共生菌-昆虫-植物互作关系研究进展显示文摘在长期的协同进化过程中,昆虫与其体内的共生菌建立了密切的互利共生关系。昆虫内共生菌不仅能调控宿主昆虫的营养代谢和生殖代谢,还能协助昆虫抵御生物、非生物胁迫,提高昆虫对化学农药的抗性及对寄主植物的适应性等。因此,内共生菌是宿主昆虫生长发育及适应性的重要调控因子。目前,随着组学技术的不断发展,内共生菌在宿主昆虫和寄主植物中的原位功能不断被挖掘,通过对内共生菌-昆虫-植物互作模型的研究,将进一步揭示昆虫内共生菌与昆虫、植物的互作机理,加深对昆虫适应性机制的理解并推进新型害虫防控和靶标技术的研发。本文就昆虫内共生菌的起源、特点、分布和传递,昆虫内共生菌在昆虫-植物-环境互作中的作用,以及昆虫内共生菌研究的方法和新技术等方面进行了综述,并对未来昆虫内共生菌介导的防御效应及昆虫适应性机理等热点问题进行了展望。 | 李迁 范佳 孙京瑞 王满囷 Frederic Francis 陈巨莲 | 2016 | 植物保护学报2016,43,6: | 18 |
| 3 | 麦田间作对麦长管蚜的生态调控作用显示文摘[目的]探讨麦田间作对麦长管蚜种群的生态调控作用。[方法]田间调查小麦单作田、小麦与绿豆、小麦与豌豆以8:2行距间作田麦长管蚜种群数量及其时间动态变化;室内利用'Y'型嗅觉仪测定麦长管蚜对小麦、小麦与绿豆、小麦与豌豆不同组合气味的选择性。[结果]麦田间作不能影响麦蚜种群随时间动态变化曲线,但能在一定程度上降低麦长管蚜无翅蚜和有翅蚜的数量。室内麦长管蚜无翅蚜和有翅蚜显著选择寄主植物的气味。当小麦与绿豆或小麦与豌豆组合时对麦长管蚜没有吸引作用。[结论]非寄主植物的气味对寄主植物气味起掩盖作用,干扰蚜虫的寄主定位,这可能是间作低于单作蚜量的原因之一。 | 解海翠 陈巨莲 程登发 周海波 孙京瑞 刘勇 Frederic Francis | 2012 | 植物保护2012,38,1: | 12 |
| 4 | Role of terpenes from aphid-infested potato on searching and oviposition behavior of Episyrphus balteatus显示文摘应付病原体和昆虫攻击,植物开发防卫的不同机制,在两个直接(物理、化学) 并且间接方法(到食虫的 beneficials 的吸引人的 volatiles ) 。植物当时能表示由吸引草食动物食肉动物便于害虫的“自顶向下”的控制的特点。这里,我们由分析不稳定的模式调查土豆植物的间接防卫机制健康并且 aphid-infestedplants。在由 Myzus persicae 的射出的萜烯模式的重要变化大批出没于寄植物被观察。用稳固的阶段 MicroExtraction (SPME ) 和 GCMS,(E)-beta-farnesene (EBF ) 看起来被大批出没于蚜虫的土豆并且不由健康植物射出。为了在蚜虫以后估计这些不稳定的版本的 theinfochemical 角色,在 aphidophagous predatorsEpisyrphus balteatus 上损坏, hover 苍蝇 foraging 行为用观察员 5.0 软件被估计(Noldus, Wageningen,荷兰) 。没有蚜虫的土豆植物也被用作控制在食肉动物的不稳定的来源行为的学习。当大批出没于蚜虫的植物导致了有效寻找并且公认字义行为导致时生蛋,健康土豆植物的 kairomonal 效果都没被观察,在网笼子导致更长的不动持续时间和更短的寻找时期。E 的 Highoviposition 率。当大批出没于蚜虫的土豆被使用时, balteatus 被观察(每放并且每 48.9eggs 意味着女性) 。在另一方面,没有鸡蛋被 hover 苍蝇在 healthyaphid 免费的植物上生产。E。balteatus foraging 和繁殖行为在综合蚜虫害虫管理在与 activeinfochemical 分子的潜在的使用的关系根据从大批出没于蚜虫的植物的不稳定的排放被讨论。 | NICOLAS HARMEL RAKI ALMOHAMAD MARIE-LAURE FAUCONNIER PATRICK DU JARDIN FRANCOIS VERHEGGEN MICHEL MARLIER ERIC HAUBRUGE FREDERIC FRANCIS | 2007 | Insect Science2007,14,1: | 11 |
| 5 | 植物防御信号物质JA/SA对桃蚜解毒酶谷胱甘肽-S-转移酶及唾液腺基因C002表达诱导反应显示文摘茉莉酸(JA)与水杨酸(SA)为植物体内重要的防御反应信号物质,在植株受到机械损伤或病虫害侵害时可作为信号物质,激活下游相关防御反应.为应对植株的防御反应,昆虫通常通过提高自身相关解毒酶活性或分泌唾液蛋白调节寄主防御反应以增强对寄主植株适应性.本研究以桃蚜(Myzus persicae(Sulzer))体内的重要解毒酶谷胱甘肽-S-转移酶(GST)的sigma型及唾液腺特异表达基因C002为研究对象,分别以含有5 mmol/L JA或10 mmol/L SA的人工饲料直接饲喂桃蚜,在不同的取食时间点收集蚜虫,通过荧光定量PCR检测JA或SA对目的基因sigma GST及C002表达诱导反应.结果发现,当桃蚜直接取食含有JA或SA的人工饲料后,sigma GST及C002表达量都显著升高.表明,桃蚜可直接利用寄主植株防御反应信号物质JA或SA,提高体内相关解毒酶或者唾液蛋白基因表达量,以提高对寄主防御的适应性.为进一步开展桃蚜对植物防御反应适应性研究提供新的思路. | 张勇 范佳 赵兴延 刘勇 孙京瑞 Frederic Francis 陈巨莲 | 2016 | 中国科学:生命科学2016,46,5: | 10 |
| 6 | Performances of local poultry breed fed black soldier fly larvae reared on horse manure显示文摘In poultry,feed based on maggots,like larvae of black soldier fly(Hermetia illucens) is an attractive option to substitute current ingredients which are expensive and often in direct or indirect competition with human food.Little information is currently available on the utility of these larvae in poultry feed,so goals of this study were to determine whether larvae could be reared on horse manure under traditional farming conditions and to evaluate the growth performances of a local poultry fed these larvae and the fatty acids profiles of their meat.After freezing and thawing,larvae were introduced in the feed of Ardennaise chickens between 30 and 80 days of age.Birds in the control group received a commercial standard feed,while those in the treatment group received the same commercial feed in which 8% was substituted with whole fresh larvae corresponding to 2% on a dry matter basis.Means ± standard errors of larval length and weight were 20.67 ± 2.21 mm and 0.14 ± 0.02 g,respectively.Mean larval percentages of dry matter and of substances extractable in diethyl ether were 24.6% and 23.1%,respectively.Larval fatty acids profiles were predominantly composed of lauric acid(28.1%) and palmitic acid(22.0%).Least squares means of weekly weights of chicken,adjusted for the effects of sex,replication and initial weights,were significantly higher(P < 0.05) by 77.03 ± 53.37 g in larvae-fed than in control chickens.All other measurements were not statistically different between larvae-fed and control chicken,including fatty acid profiles,protein content and w6/w3 ratio. | Nassim Moula Marie-Louise Scippo Caroline Douny Guy Degand Edwin Dawans Jean-Francois Cabaraux Jean-Luc Hornick Rudy C.Medigo Pascal Leroy Frederic Francis Johann Detilleux | 2018 | Animal Nutrition2018,,1: | 7 |
| 7 | 枫长镰管蚜气味结合蛋白OBP3的cDNA克隆和序列分析及其成虫嗅觉行为反应测定显示文摘枫长镰管蚜Drepanosiphum platanoidis是少数几种不分泌E-β-法尼烯(E-β-farnesene,EBF)的蚜虫之一。ApisOBP3是首个被鉴定的特异结合EBF的昆虫气味结合蛋白,来自于豌豆蚜Acyrthosiphum pisum。本研究通过基因克隆技术,在枫长镰管蚜体内获得2个表达ApisOBP3类似蛋白的cDNA序列,长度均为426bp,其中,5'末端的69bp为信号肽编码序列。编码的成熟蛋白分别命名为DplaOBP3-1和DplaOBP3-2,两个蛋白均由119个氨基酸残基组成,序列一致性为95.8%。与ApisOBP3的序列一致性分别为95.8%和98.3%。DplaOBP3-1,DplaOBP3-2及ApisOBP33个蛋白具有数量和位置完全一致的6个保守的半胱氨酸残基。以测得3D结构的马德拉蜚蠊Leucophaea maderae的信息素结合蛋白LmadPBP为模板比较建模,结果显示两个蛋白在疏水中心均具有与ApisOBP3一致的关键疏水残基及芳香族氨基酸残基Tyr84。利用四臂嗅觉仪测定枫长镰管蚜的嗅觉行为反应,结果显示:枫长镰管蚜残体挥发物及外源EBF对该蚜均有显著的驱避作用(P=0.01)。本研究提示,枫长镰管蚜具有种内报警信息素,该蚜残体挥发物即为其种内报警信息素;枫长镰管蚜尽管并不分泌EBF,但具有表达2种OBP3蛋白的基因,并可识别环境中的EBF且对其作出行为反应。 | 范佳 Sophie VANDERMOTEN Frederic FRANCIS 刘勇 陈巨莲 程登发 | 2011 | 昆虫学报2011,54,9: | 6 |
| 8 | Testing semiochemicals from aphid, plant and conspecific: attraction of Harmonia axyridis显示文摘Harmonia axyridis 智慧女神雅典娜(翘目:Coccinellidae ) 是影响几个行业协会的动力学和作文的一个侵略硬币。现在,没有生物控制方法是可得到的减少这有害 coccinellid 的人口。引诱剂和 semiochemicals 似乎是到是最好的选择,但是仅仅很少研究在这亚洲女士甲壳虫上测试了 semiochemicals 的影响。在这个工作,通过风隧道实验,从蚜虫的 semiochemicals (Z, E-nepetalactone,[E ]-- 从 coccinellids 的 farnesene, -pinene 和 -pinene),([-]--caryophyllene) 并且从荨麻 Urtica dioica L。作为潜在的引诱剂被评估。荨麻不稳定的混合物([Z ]-3-hexenol 并且[E ]-2-hexenal) 用一台 Clevenger 仪器被提取?并且由 headspace 气体 chromatographymass 光谱学识别了。当时,在风隧道实验,蚜虫警报 pheromone 的主要部件以及蚜虫的一个部件,性 pheromone 强烈吸引了亚洲女士甲壳虫的两性(-)-- caryophyllene 仅仅吸引了很少个人并且没在男性上有影响。象(Z)-3-hexenol 一样的荨麻摘录面向到气味的男性和女性采购原料。就算,(E)-2-hexenal 被显示没在女性上有效果这片绿叶不稳定的吸引的男性。因为 Z, E-nepetalactone 在风隧道实验作为最有效的引诱剂被识别,这不稳定也在一块土豆地里被测试在哪儿 H。axyridis 被显示了对这 semiochemical 作出回应。这研究加亮那 Z, E-nepetalactone 面向了亚洲女士甲壳虫 H。在天赋下面的 axyridis 调节,显示这不稳定的混合物能当然为有效生物控制帮助对这个侵略硬币来临。 | Pascal D. Leroy Thomas Schillings Julien Farmakidis Stephanie Heuskin Georges Lognay Francois J. Verheggen Yves Brostaux Eric Haubruge Frederic Francis | 2012 | Insect Science2012,19,3: | 5 |
| 9 | 小麦品种(系)对不同地理种群荻草谷网蚜的抗性显示文摘采用田间成株期自然感蚜法和室内苗期接虫法,鉴定12个小麦品种(系)对5个不同地区荻草谷网蚜Sitobion miscanthi(Takahashi)种群的抗性。结果表明,小麦品种(系)室内苗期的抗性表现与田间成株期基本一致;Amigo、KOK、北京837、铭贤169和红芒红对5个地理种群荻草谷网蚜的抗性表现相同,丰产3、中4无芒、JP①、晋麦31、L1、885479-2和小白冬麦对5个地理种群荻草谷网蚜种群的抗性表现则存在差异。研究表明我国荻草谷网蚜对抗蚜作物品种具有致害性分化。 | 徐昭焕 程登发 孙京瑞 刘勇 杨秀丽 FREDERIC Francis 陈巨莲 | 2012 | 应用昆虫学报2012,49,3: | 4 |
| 10 | Bacteria may contribute to distant species recognition in ant-aphid mutualistic relationships显示文摘在蚂蚁和蚜虫种类之间的 Mutualistic 相互作用是可观的历史、当代的调查的题目,主要好处正在清洗并且为蚜虫的保护和为蚂蚁的充满糖类的蜜露。然而,问题至于影响这种关系的不稳定的 semiochemical 因素留下了。最近的研究加亮细菌的蜜露的角色在蚂蚁吸引力的不稳定的混合物。这里,远地区别 2 蚜虫种类的蚂蚁能力用双向 olfactometer 基于细菌的蜜露 semiochemicals 排出物被调查。两 mutualistic 蚜虫蚜虫属 fabae L。并且 nonmyrmecophilous 蚜虫 Acyrthosiphon pisum 哈里斯被发现为蚂蚁 Lasius 尼日尔漂亮 L。吸引力的水平在两试金是类似的(控制对蚜虫种类之一) 。不管多么当给在这之间的一种选择时 2 蚜虫种类,蚂蚁为蚜虫属 fabae 显示出重要偏爱。主要从细菌的起源,蜜露 volatiles 被知道是在蚂蚁吸引力的一个关键元素。用一样的 olfactometry 协议,蜜露射出的 volatiles 的相对优美从每蚜虫种收集了,由从各个孤立的细菌,蜜露被调查。再次,蚂蚁显著地比较喜欢蚜虫属 fabae 蜜露和细菌释放的 volatiles。这个信息建议微生物引起的蜜露 volatiles 使蚂蚁能远地区别蚜虫种类。这些结果加强在昆虫 symbioses 学习出现和微生物的潜在的影响的兴趣。 | Christophe Y. Fischer Claire Detrain Philippe Thonart Eric Haubruge Frederic Francis Francois J. Verheggen Georges C. Lognay | 2017 | Insect Science2017,24,2: | 3 |
| 11 | Aphidophagous guilds on nettle (Urtica dioica) strips close to fields of green pea, rape and wheat显示文摘普通荨麻(Urtica dioica L.) 是长期、大都会的植物种并且被知道是为昆虫的大差异的食物的来源。在agro生态系统理解荨麻的重要性,一个地实验在 Gembloux (比利时)在一个试验性的农场被执行在靠近的旱田罪在蚜虫和 aphidophagous 人口上学习荨麻边缘脱衣的效果,也就是小麦( Triticum aestivwn L.),绿豌豆( Pisum sativum L.)和强奸( Brassicae 马来麝L.)。蚜虫和相关有益的人口每周被估计,从三月到 2005 年 8 月,由在每旱田罪的二个阴谋的视觉观察。更高的许多 aphidophagous beneficials 在荨麻脱衣被收集什么时候与旱田罪相比。特别地,食肉的 anthocorids,米尔标志和绿草蜻蛉的存在仅仅在荨麻上被观察。不过,最丰富的蚜虫食肉的家庭, Coccinellidae,在两环境,在荨麻脱衣并且在庄稼地里被散布。地边缘比旱田罪支持了 Harmonia axyridis 的显著地更高的密度。相反,旱田罪,绿豌豆特别地,支持了 Coccinella septempunctata 的更高的密度。aphidophagous 种类的分发,主要瓢虫,在综合害虫管理上在与寄植物和相关蚜虫种类和他们的潜在的效果的关系被讨论。 | AMMAR ALHMEDI ERIC HAUBRUGE BERNARD BODSON FREDERIC FRANCIS | 2007 | Insect Science2007,14,5: | 3 |
| 12 | 蛋白质激发子Hrip1对小麦黄矮病的诱抗作用显示文摘小麦黄矮病是由大麦黄矮病毒(barley yellow dwarf virus,BYDV)引起的一种小麦病毒病,其传播介体是小麦蚜虫,在小麦生产中造成巨大的经济损失。近年来,植物诱导抗性作为一种新兴的植物病虫害防治措施引起了广泛的关注。蛋白质激发子Hrip1可以激活多种植物的免疫防御反应诱导植物产生广谱抗性。本研究评价了Hrip1对小麦黄矮病的诱抗效果。用30μg/m L的Hrip1溶液进行小麦浸种和幼苗喷雾,随后接种BYDV,接种后第14 d,Hrip1对小麦黄矮病控制效果在50%以上,接种后第21 d控制效果仍在30%以上。实时荧光定量PCR检测结果显示,在Hrip1处理的小麦幼苗体内,BYDV外壳蛋白m RNA的数量显著低于对照组;EPG结果显示,在Hrip1处理的小麦幼苗上,麦二叉蚜寻找叶片刺吸位点和韧皮部取食位点的时间增加。以上结果表明:Hrip1能够有效地抑制BYDV在小麦体内的增殖;影响传毒媒介麦二叉蚜的取食行为,抑制其传毒能力。此外,Hrip1处理小麦能有效缓解BYDV引起的叶片黄化和植株矮化的症状。因此,Hrip1可以作为生物诱导剂综合控制小麦黄矮病。 | 李琳 王双超 杨秀芬 Frederic FRANCIS 邱德文 | 2020 | 中国生物防治学报2020,36,1: | 2 |
| 13 | Eedoscopic lithotripsy and the FREDDY laser :initial esperience 显示文摘 | Francis D Federico P Frederic G | 2006 | Journal of endourology2006,20,5: | 1 |
| 14 | Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum显示文摘Pea aphid,Acyrthosiphon pisum,is a serious pest of many different leguminous plants,and it mainly relies on its odorant receptors(Ors)to discriminate among host species.However,less is known about the role that Ors play in the host plant location.In this study,we identified a novel conserved odorant receptor clade by phylogenetic analysis,and conducted the functional analysis of ApisOr23 in A.pisum.The results showed that the homologous Ors from A.pisum,Aphis glycines and Aphis gossypii share 94.28% identity in amino acid sequences.Moreover,conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species,providing further proof of the high conservation level of the Or23 clade.According to the tissue expression pattern analysis,ApisOr23 was mainly expressed in the antennae.Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles,namely trans-2-hexen-1-al,cis-2-hexen-1-ol,1-heptanol,4’-ethylacetophenone,and hexyl acetate.Among them,trans-2-hexen-1-al,which is one of the main volatile organic compounds released from legume plants,activated the highest response of ApisOr23.Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection. | HUANG Tian-yu ZHANG Rui-bin YANG Lu-lu CAO Song Frederic FRANCIS WANG Bing WANG Gui-rong | 2022 | Journal of Integrative Agriculture2022,21,5: | 1 |
| 15 | Immobilization of multifunctional Schiff base containing ruthenium complexes on MCM-41显示文摘 | Clercq Bob De Lefebvre Frederic Verpoort Francis | 2003 | Applied Catalysis A2003,,247: | 1 |
| 16 | Endoscopic lithotripsy and the FREDDY laser:initial esperience显示文摘 | Francis D Federico P Frederic G | 2006 | Journal of Endourology2006,20,5: | 1 |
| 17 | Role of endocrine status and cell type in adhesion of human endometrial cells to the peritoneum in nude mice显示文摘 | Aude Beliard Agnes No?l Frederic Goffin Francis Frankenne Jean-Michel Foidart | 2002 | Fertility and Sterility2002,,5: | 1 |
| 18 | The ectomycorrhizal symbiosis:genetics and development显示文摘 | DENIS T FREDERIC L FRANCIS M | 2002 | Plant and Soil2002,244,35: | 1 |
| 19 | A conserved odorant receptor identified from antennal transcriptome of Megoura crassicauda that specifically responds to cis-jasmone显示文摘Herbivore-induced plant volatiles(HIPVs)play a key role in the interactions between plants and herbivorous insects,as HIPVs can promote or deter herbivorous insects’behavior.While aphids are common and serious phloem-feeding pests in farmland ecosystems,little is known about how aphids use their sensitive olfactory system to detect HIPVs.In this study,the antennal transcriptomes of the aphid species Megoura crassicauda were sequenced,and expression level analyses of M.crassicauda odorant receptors(ORs)were carried out.To investigate the chemoreception mechanisms that M.crassicauda uses to detect HIPVs,we performed in vitro functional studies of the ORs using 11 HIPVs reported to be released by aphid-infested plants.In total,54 candidate chemosensory genes were identified,among which 20 genes were ORs.McraOR20 and McraOR43 were selected for further functional characterization because their homologs in aphids were quite conserved and their expression levels in antennae of M.crassicauda were relatively high.The results showed that McraOR20 specifically detected cis-jasmone,as did its ortholog ApisOR20 from the pea aphid Acyrthosiphon pisum,while McraOR43 did not respond to any of the HIPV chemicals that were tested.This study characterized the ability of the homologous OR20 receptors in the two aphid species to detect HIPV cis-jasmone,and provides a candidate olfactory target for mediating aphid behaviors. | WANG Bo HUANG Tian-yu YAO Yuan Frederic FRANCIS YAN Chun-cai WANG Gui-rong WANG Bing | 2022 | Journal of Integrative Agriculture2022,21,7: | 0 |
| 20 | Transcriptome analysis reveals different response of resistant and susceptible rice varieties to rice stripe virus infection显示文摘Rice stripe disease,caused by rice stripe virus (RSV) which is transmitted by small brown planthopper (SBPH,Laodelphax striatellus Fallen),resulted in serious losses to rice production during the last 2 decades.Research on the molecular differences between resistant and susceptible rice varieties and the interaction between rice and RSV remains inadequate.In this study,RNA-Seq was used to analyze the transcriptomic differences between the resistant and susceptible rice varieties at different times post RSV infection.Through Gene Ontology (GO) annotation,the differentially expressed genes (DEGs) related to transcription factors,peroxidases,and kinases of 2 varieties at 3 time points were identified.Comparing these 2 varieties,the DEGs associated with these 3 GOs were numerically less in the resistant variety than in the susceptible variety,but the expression showed a significant up-or down-regulation trend under the conditions of|log_2(Fold change)|>0&P_(adj)<0.05 by significance analysis.Then through Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation,DEGs involved in some pathways that have a contribution to disease resistance including plant hormone signal transduction and plant–pathogen interaction were found.The results showed that resistance responses regulated by abscisic acid (ABA) and brassinosteroids (BR) were the same for 2 varieties,but that mediated by salicylic acid (SA) and jasmonic acid (JA)/ethylene (ET) were different.The DEGs in resistant and susceptible varieties at the 3 time points were identified in both PAMP-triggered immunity (PTI) and Effector proteintriggered immunity (ETI),with that most of the unigenes of the susceptible variety were involved in PTI,whereas most of the unigenes of the resistant variety were involved in ETI.These results revealed the different responses of resistant and susceptible varieties in the transcription level to RSV infection. | LIU Yu LIU Wen-wen LI Li Frederic FRANCIS WANG Xi-feng | 2023 | Journal of Integrative Agriculture2023,22,6: | 0 |