|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 2 | 短期常压低氧步行训练对肥胖成年人运动能力和步态的影响显示文摘为了比较以个人喜好的步行速度(preferred walking speed,PWS)短期低氧与常氧训练对肥胖患者的能量、力学、效率和代谢风险标志物的影响,Fernández Menéndez等纳入肥胖患者23例,在缺氧[海拔3km,n=12;体质量指数(body mass index,BMI)(34.0±0.8)kg/m2]或常氧[海拔360m,n=11;BMI(32.9±0.8)kg/m2]情况下. | 刘青 叶鹏 Fernández Menéndez A Saudan G Sperisen L Hans D Saubade M Millet GP Malatesta D | 2018 | 中华高血压杂志2018，26，6： | 2 |
| 3 | Blatella germanica as a possible cockroach vector of micro-organisms in a hospital显示文摘 | G. Risco Oliva C. Díaz O. Fuentes González M.D. Martínez C. Fernández R. Cordoví P. Más Lago N. Herrera | 2009 | Journal of Hospital Infection2009，，1： | 2 |
| 4 | Effect of freezing fish skins on molecular and rheological properties of extracted gelatin显示文摘 | P Montero M D Fernández-Díaz M C Gómez-Guillén | 2003 | Food Hydrocolloids2003，17，5： | 1 |
| 5 | The use of knowledge for technological innovation within diversified firms显示文摘 | Miller D J Fern M J Cardinal L B | 2007 | Academy of Management Journal2007，50，2： | 1 |
| 6 | The use of knowledge fortechnological innovation within diversified firms 显示文摘 | Miller D J Fern M J Cardinal L B | 2007 | Academy ofManagement Journal2007，50，2： | 1 |
| 7 | Tox- icity evaluation of single and mixed antifouling biocides measured with acute toxicity bioassays 显示文摘 | Fern ndez-Alba A R Hernando M D Piedra L | 2002 | Anal Chim Ac- ta2002，456，： | 1 |
| 8 | Optimization of microwave-assisted extraction of hydrocarbons in marine sediments : Comparison with the Soxhlet extraction method显示文摘 | Blanco E V Mahia P L Lorenzo S M Rodr guez D P Fern ndez E F | 2000 | Fresenius Journal of Analytical Chemistry2000，366，： | 1 |
| 9 | The use of knowledge for technological innovation within diversified firms显示文摘 | Miller D J Fern M J Cardinal L B | 2007 | The Academy of Management Journal2007，50，2： | 1 |
| 10 | The use of knowledge for technological innovation within diversified firms 显示文摘 | Miller D J Fern M J Cardinal L B | 2007 | Academy of Management Journal2007，50，2： | 1 |
| 11 | Antioxi dant activity of wines and relation with their potyphenolie composition显示文摘 | FERNNDEZ-PACHN M S VILLANO D GARCIA-PARILLA M C | 2004 | Analytica chirnicacta2004，513，： | 1 |
| 12 | Effects of different dietary protein and lipid levels on growth, feed utilization and body composition of red porgy (Pagrus pagrus) fingerlings 显示文摘 | SCHUCHARDT D VERGARA J M FERN NDEZ-PALACIOS H | 2008 | Aquaculture Nutrition2008，14，1： | 1 |
| 13 | Internalgeometry variation of LTCC inductors to improvelight-Load efficiency of DC-DC converters显示文摘 | Lim M Hui Fern Wyk J D van Zhenxian L | 2009 | IEEETransactions on Components and Packaging Techno-logies2009，32，： | 1 |
| 14 | Micelle formation for improvement of continuous subcritinal water extraction of polycyclic aromatic hydrocarbons in soil prior to high-performance liquid chromatography-fluorescence detection显示文摘 | FERNαNDEZ-PERéZ V LUQUE DE CASTRO M D | 2000 | Journal of Chromatography A2000，902，： | 1 |
| 15 | On the complexity and dynamics of in vivo CisplatinDNA adduct formation using HPLC/ICPMS显示文摘 | Ziehe M EstebanFernández D Hochkirch U | 2012 | Metallomics2012，4，10： | 1 |
| 16 | Real time quantitative PCR detection of histamine-producing lactic acid bacteria in cheese:Relation with histamine content显示文摘 | LADERO V LINARES D M FERNNDEZ M | 2008 | Food Research International2008，41，10： | 1 |
| 17 | The Use of Knowledge for Technological Innovation with- in Diversified Firms 显示文摘 | kMILLER D J FERN M J CARDINAL L B | 2007 | Academy of Management Journal2007，50，2： | 1 |
| 18 | Contribution of OqxAB efflux pumps to quinolone resistance in extendedspectrum-β-lactamase-producing Klebsiella pneumoniae显示文摘 | Rodríguez-Martínez JM Díaz de Alba P Briales A Machuca J Lossa M Fernández-Cuenca F RodríguezBa(n)o J Martínez-Martínez L Pascual (A) | | 0，，1： | 1 |
| 19 | Femoral oxygenation during hip resurfaeing through the troehanterie flip approach 显示文摘 | Steffen R Fern D Norton M | 2009 | Clin Orthop Relat Res2009，467，： | 1 |
| 20 | Zinnia elegans uses the same peroxidase isoenzyme complementfor cell wall lignification in both single-cell tracheary elementsand xylem vessels显示文摘 | L PEZ-SERRANO M FERN NDEZ M D POMAR F | 2004 | Journal of Experimental Botany2004，55，396： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员