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| 1 | Establishment and evaluation of a peanut association panel and analysis of key nutritional traits显示文摘Breeding programs aim to improve the yield and quality of peanut(Arachis hypogaea L.); using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for association analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure(Q) analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship(K) indicated that most of the panel members have no or weak familial relatedness, with 52.78% of lines showing K ? 0. Linkagedisequilibrium(LD) analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q t K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis,providing a resource for marker-assisted selection for peanut improvement. | Xiurong Zhang Suqing Zhu Kun Zhang Yongshan Wan Fengzhen Liu Qingfang Sun Yingjie Li | 2018 | Journal of Integrative Plant Biology2018，60，3： | 5 |
| 2 | Carbon,nitrogen and phosphorus stoichiometry in Pinus tabulaeformis forest ecosystems in warm temperate Shanxi Province,north China显示文摘Although carbon(C), nitrogen(N), and phosphorous(P) stoichiometric ratios are considered good indicators of nutrient excess/limitation and thus of ecosystem health, few reports have discussed the trends and the reciprocal effects of C:N:P stoichiometry in plant–litter–soil systems. The present study analyzed C:N:P ratios in four age groups of Chinese pine, Pinus tabulaeformis Carr., forests in Shanxi Province, China: plantation young forests(AY,<20 year-old); plantation middle-aged forests(AM, 21–30 year-old); natural young forests(NY,<30 year-old); and natural middle-aged forests(NM,31–50 year-old). The average C:N:P ratios calculated for tree, shrub, and herbaceous leaves, litter, and soil(0–100 cm) were generally higher in NY followed by NM,AM, and AY. C:N and C:P ratios were higher in litter than in leaves and soils, and reached higher values in the litter and leaves of young forests than in middle-aged forests;however, C:N and C:P ratios were higher in soils of middle-aged forests than in young forests. N:P ratios were higher in leaves than in litter and soils regardless of stand age; the consistent N:P<14 values found in all forests indicated N limitations. With plant leaves, C:P ratios were highest in trees, followed by herbs and shrubs, indicating a higher efficiency in tree leaf formation. C:N ratios decreased with increasing soil depth, whereas there was no trend for C:P and N:P ratios. C:N:P stoichiometry of forest foliage did not exhibit a consistent variation according to stand age. Research on the relationships between N:P, and P, N nutrient limits and the characteristics of vegetation nutrient adaptation need to be continued. | Ning Wang Fengzhen Fu Baitian Wang Ruijun Wang | 2018 | Journal of Forestry Research2018，29，6： | 4 |
| 3 | Surface analysis and electrochemical behaviour of the self-assembled polydopamine/dodecanethiol complex films in protecting 304 stainless steel显示文摘A self-assembled 1-dodecanethiol film assisted with the preferential adhesion of polydopamine was prepared on the non-etching 304 stainless steel surfaces by a simple dip-coating method.The formation and surface structure of the film were characterized by water contact angle measurement,atomic force microscopy(AFM),and X-ray photoelectron spectroscopy(XPS).The corrosion behavior of the complex films was evaluated by Tafel polarization curve and electrochemical impedance spectroscopy(EIS).The excellent corrosion resistance property could be attributed to the compact hybrid film structure and superior seawater stability for modified 304 stainless steel surface. | CHEN YuanYuan CHEN ShouGang CHEN Yan SONG XianWang XU FengZhen | 2012 | Science China(Technological Sciences)2012，55，6： | 4 |
| 4 | Molecular analysis of the chloroplast Cu/Zn-SOD gene(AhCSD2) in peanut显示文摘Superoxide dismutase(SOD, EC 1.15.1.1) plays a key role in response to drought stress, and differences in SOD activity changes among cultivars are important under drought conditions. We obtained the full-length DNA of the chloroplast Cu/Zn-SOD gene(Ah CSD2)from 11 allotetraploid cultivars and 5 diploid wild species in peanut. BLAST search against the peanut genome showed that the Ah CSD2 genes g CSD2-1 and g CSD2-2 are located at the tops of chromosome A03(A genome) and B03(B genome), respectively, and both contain 8exons and 7 introns. Nucleotide sequence analyses indicated that g CSD2-2 sequences were identical among all the tested cultivars, while g CSD2-1 sequences showed allelic variations.The amino acid sequences deduced from g CSD2-1 and g CSD2-2 both contain a chloroplast transit peptide and are distinguished by 6 amino acid(aa) residue differences. The other 2aa residue variations in the mature peptide regions give rise to three-dimensional structure changes of the protein deduced from the genes g CSD2-1 and g CSD2-2. Sequences analyses of cultivars and wild species showed that g CSD2-2 of Arachis hypogaea and g Aip CSD2(Arachis ipaensis) are identical, and despite the abundant polymorphic loci between g CSD2-1 of A.hypogaea and sequences from A genome wild species, the deduced amino acid sequence of Ah CSD2-1(A. hypogaea) is identical to that of Adu CSD2(Arachis duranensis), whereas Aco CSD2(Arachis correntina) and Aca CSD2(Arachis cardenasii) both have 2 aa differences in the transit peptide region compared with Ah CSD2-1(A. hypogaea). Based on the Peanut Genome Project, promoter prediction revealed many stress-related cis-acting elements within the potential promoter regions(pp-A and pp-B). pp-A contains more binding sites for drought-associated transcriptional factors than pp-B. We hypothesize that the marked changes in SOD activity in different cultivars under drought stress are tightly regulated by transcription factors through transcription and expression of Ah CSD2 genes. | Xiurong Zhang Qian Wan Fengzhen Liu Kun Zhang Aiqing Sun Bing Luo Li Sun Yongshan Wan | 2015 | The Crop Journal2015，3，3： | 4 |
| 5 | Erosive Wear and Wear Mechanism of in situ TiC_P/Fe Composites显示文摘The base structure of in situ TiCp/Fe composites fabricated under industrial condition was changed by differentheat treatments. Erosive wear tests were carried out and the results were compared with that of wear-resistant whitecast iron. The results suggest that the wear resistance of the in situ TiCP/Fe composite is higher than that ofwear-resistant white cast iron under the sand erosive wear condition. The wear mechanism of the wear-resistantwhite cast iron was a cycle process that base surface was worn and carbides were exposed, then carbides was brokenand wear pits appeared. While the wear mechanism of in situ TiCP/Fe composite was a cycle process that basesurface was worn and TiC grains were exposed and dropped. The wear resistance of in situ TiCP/Fe composite waslower than that of wear-resistant white cast iron under the slurry erosive wear condition. Under such circumstance,the material was not only undergone erosive wear but also electrochemistry erosion due to the contact with waterin the medium. The wear behaviours can be a combination of two kinds of wear and the sand erosive wear is worsethan slurry erosive wear. | Zhaojing LIU, Zhiliang NING , Fengzhen LI, Xiurong YAO and Shanzhi RENSchool of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150080, China | 2005 | Journal of Materials Science & Technology2005，21，5： | 3 |
| 6 | Effects of Exogenous Nitric Oxide and 24-Epibrassinolide on the Physiological Characteristics of Peanut Seedlings Under Cadmium Stress显示文摘Cadmium(Cd) is highly toxic to plants, animals, and humans. Limited information is available on the role of nitric oxide(NO)and/or 24-epibrassinolide(EBR) in response of plants to Cd stress. In this study, a hydroponic experiment was performed to investigate the effects of NO and/or EBR on peanut plants subjected to Cd stress(200 μmol L^(-1)) with sodium nitroprusside(SNP, an exogenous NO donor)(250 μmol L^(-1)) and/or EBR(0.1 μmol L^(-1)) addition. The results showed that Cd exposure inhibited plant growth, and this stress was alleviated by exogenous NO or EBR, and especially the combination of the two. Treatment with Cd inhibited the growth of peanut seedlings, decreased chlorophyll content, and significantly increased the Cd concentration in plants. Furthermore, the concentration of reactive oxygen species(ROS) markedly increased in peanut seedlings under Cd stress, resulting in the accumulation of malondialdehyde(MDA) and proline in leaves and roots. Under Cd stress, applications of SNP, EBR, and especially the two in combination significantly reduced the translocation of Cd from roots to leaves, increased the chlorophyll content, decreased the concentrations of ROS, MDA, and proline, and significantly enhanced the activities of superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) in peanut seedlings. Exogenous NO and/or EBR also stimulated the activities of nitrate reductase(NR)and nitric oxide synthase(NOS) and increased the contents of antioxidants, such as ascorbic acid(AsA) and reduced glutathione(GSH). Furthermore, exogenous NO and/or EBR enhanced Cd accumulation in the cell wall and thus decreased Cd distribution in the organelles in the roots. The concentrations of calcium(Ca), iron(Fe), magnesium(Mg), and zinc(Zn) were also regulated by exogenous NO or EBR, and especially by the two in combination. These results indicated that SNP and EBR, alone and particularly in combination, can mitigate the negative effects of Cd stress in peanut plants. | DONG Yuanjie CHEN Weifeng BAI Xiaoying LIU Fengzhen WAN Yongshan | 2019 | Pedosphere2019，29，1： | 3 |
| 7 | Determining the critical size of a rabbit rib segmental bone defect model显示文摘In order to establish and standardize the rabbit rib segmental bone defect model,it is of vital importance to determine rabbit rib critical size defect(CSD).According to the general time needed for spontaneous long-bone regeneration,three-month observation period was set to determine the CSD.The rabbit rib segmental bone defects with different sizes from 1 to 5cm with or without periosteum were performed in the eighth rib of 4-month-old male New Zealand rabbits and underwent Xray examinations at the 4th,8th and 12th weeks postoperatively.The gross and histological examinations at postoperative week 12 were evaluated,which showed that the critical sizes in the rabbit rib models with and without periosteum were 5 and 2 cm,respectively.This study provides prerequisite data for establishing rabbit rib CSD model and evaluating bonematerials using this model. | Fengzhen Liu Kun Chen Lei Hou Keyi Li Dawei Wang Bin Zhang Xiumei Wang | 2016 | Regenerative Biomaterials2016，3，5： | 2 |
| 8 | Dynamics and manipulation of ferroelectric domain walls in bismuth ferrite thin films显示文摘Ferroelectric domain walls differ from domains not only in their crystalline and discrete symmetry,but also in their electronic,magnetic,and mechanical properties.Although domain walls provide a degree of freedom to regulate the physical properties at the nanoscale;the relatively lower controllability prevents their practical applications in nano-devices.In this work;with the advantages of 3D domain configuration detection based on piezoresponse force microscopy;we find that the mobility of three types of domain walls(tail-to-tail,head-to-tail,head-to-head)in(001)BiFeO3 films varies with the applied electrical field.Under low voltages;head-to-tail domain walls are more mobile than other domain walls;while,under high voltages,tail-to-tail domain walls become rather active and possess relatively long average lengths.This is due to the high nucleation energy and relatively low growth energy for charged domain walls.Finally;we demonstrate the manipulation of domain walls through successive electric writings,resulting in well-aligned conduction paths as designed;paving the way for their application in advanced spintronic;memory and communication nano-devices. | Shuyu Xiao Yaming Jin Xiaomei Lu Sang-Wook Cheong Jiangyu Li Yang Li Fengzhen Huang Jinsong Zhu | 2020 | National Science Review2020，7，2： | 2 |
| 9 | Multiscale ApEn and SampEn in Quantifying Nonlinear Complexity of Depressed MEG显示文摘Depression is a neurophysiological disorder with recurrent dysregulations of self-mental states.Multiscale Approximate entropy(ApEn)and Sample entropy(SampEn)are employed to characterize nonlinear complexity of Magnetoencephalography(MEG)of depressive patients in our contribution.SampEn shares similarities with ApEn while has better distinctions between the MEGs of depression patients and normal people.Test results prove that nonlinear complexity of the depressive MEG is lower than that of the normal subjects,indicating weaker response of depression patients to emotional stimuli,and the optimum discriminations between the depressive and healthy people lie in frontal lobe of brain which is related to emotional regulation.Our findings provide valuable information about depression,highlight the loss of nonlinear complexity in MEG of depressive patient and can be used as clinical diagnostic aids. | YAO Wenpo HU Hui WANG Jun YAN Wei LI Jin HOU Fengzhen | 2019 | Chinese Journal of Electronics2019，28，4： | 2 |
| 10 | Nanobody-based chimeric antigen receptor T cells designed by CRISPR/Cas9 technology for solid tumor immunotherapy显示文摘Chimeric antigen receptor-based T-cell immunotherapy is a promising strategy for treatment of hematological malignant tumors;however,its efficacy towards solid cancer remains challenging.We therefore focused on developing nanobody-based CAR-T cells that treat the solid tumor.CD105 expression is upregulated on neoangiogenic endothelial and cancer cells.CD105 has been developed as a drug target.Here we show the generation of a CD105-specific nanobody,an anti-human CD105 CAR-T cells,by inserting the sequences for anti-CD105 nanobody-linked standard cassette genes into AAVS1 site using CRISPR/Cas9 technology.Co-culture with CD105+target cells led to the activation of anti-CD105 CAR-T cells that displayed the typically activated cytotoxic T-cell characters,ability to proliferate,the production of pro-inflammatory cytokines,and the specific killing efficacy against CD105+target cells in vitro.The in vivo treatment with anti-CD105 CAR-T cells significantly inhibited the growth of implanted CD105+tumors,reduced tumor weight,and prolonged the survival time of tumor-bearing NOD/SCID mice.Nanobody-based CAR-T cells can therefore function as an antitumor agent in human tumor xenograft models.Our findings determined that the strategy of nanobody-based CAR-T cells engineered by CRISPR/Cas9 system has a certain potential to treat solid tumor through targeting CD105 antigen. | Fengzhen Mo Siliang Duan Xiaobing Jiang Xiaomei Yang Xiaoqiong Hou Wei Shi Cueva Jumbo Juan Carlos Aiqun Liu Shihua Yin Wu Wang Hua Yao Zihang Yu Zhuoran Tang Shenxia Xie Ziqiang Ding Xinyue Zhao Bruce D.Hammock Xiaoling Lu | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 2 |
| 11 | Preparation and Characterization of Acylated Chitosan显示文摘Fully acylated chitosan and N, N-diacyl chitosan were prepared. The products were characterized by elemental analysis, FTIR and 1H NMR. The experimental results indicate that the average degree of acylation depends on the volume ratio of pyridine to chloroform in the reaction medium, the chain length of the acylation agent used, and the molecular weight of chitosan raw materials. The XRD measurements were carried out for pure chitosan, fully acylated chitosan and N, N-diacyl chitosan to verify the crystallinity change caused by the acylation. | LIMing-ehun LIUChao XINMei-hua ZHAOHuang WANGMin FENGZhen SUNXiao-li | 2005 | Chemical Research in Chinese Universities2005，21，1： | 2 |
| 12 | Justified Knowledge显示文摘 | | 1992 | Artificial Intelligence1992，56，： | 1 |
| 13 | Analysis of heartbeat asymmetry based on multi-scale time irreversibility test显示文摘 | Hou Fengzhen Zhuang Jianjun Bian Chunhua | 2010 | PhysicaA2010，389，： | 1 |
| 14 | Vaccination with porcine reproductive and respiratory syndrome killed virus vaccine and immunostimulatory oligodeoxynucleotides induces specific immunity in piglets显示文摘 | Linghua Z Xingshan T Fengzhen Z | 2007 | Vaccine2007，2625，10： | 1 |
| 15 | Highdimensional time irreversibility analysis of human interbeatintervals显示文摘 | Hou Fengzhen Ning Xinbao Zhuang Jianjun | 2011 | Medical Engineering and Physics2011，33，5： | 1 |
| 16 | The APOE ε2 allele may decrease the age at onset in patients with spinocerebellar ataxia type 3 or Machado-Joseph disease from the Chinese Han population显示文摘 | Huirong Peng Chunrong Wang Zhao Chen Zhanfang Sun Bin Jiao Kai Li Fengzhen Huang Xuan Hou Junling Wang Lu Shen Kun Xia Beisha Tang Hong Jiang | 2014 | Neurobiology of Aging2014，，9： | 1 |
| 17 | Preparation, optimization, and characterization of chitosancoated solid lipid nanoparticles for ocular drug delivery显示文摘The present study aimed to develop and optimize chitosan coated solid lipid nanoparticles(chitosan-SLNs)encapsulated with methazolamide. Chitosan-SLNs were successfully prepared by a modified oil-in-water emulsification-solvent evaporation method with glyceryl monostearate as the solid lipid and phospholipid as the surfactant. Systematic screening of formulation factors was carried out. The optimized formula for preparation was screened by orthogonal design as well as Box-Behnken design with entrapment efficiency, particle size and zeta potential as the indexes. The entrapment efficiency of the optimized formulation(methazolamide-chitosan-SLNs)prepared was(58.5± 4.5)%,Particle size(247.7 ± 17.3) nm and zeta potential(33.5 ±3.9) mV. Transmission electron microscopy showed homogeneous spherical particles in the nanometer range. A prolonged methazolamide in vitro release profile was obtained in the optimized chitosan-SLNs suspension compared with methazolamide solution. No ocular damages were observed in the susceptibility test on albino rabbits. The results suggest that the combination of orthogonal design and Box-Behnken design is efficient and reliable in the optimization of nanocarriers, and chitosanSLNs is a potential carrier for ophthalmic administration. | Fengzhen Wang Mingwan Zhang Dongsheng Zhang Yuan Huang Li Chen Sunmin Jiang Kun Shi Rui Li | 2018 | The Journal of Biomedical Research2018，32，6： | 1 |
| 18 | Vaccination with porcine reproductive and respiratory syndrome killed virus vaccine and immunostimulatory oligodeoxynucleotides induces specific im- munityinpiglets显示文摘 | Linghua Zhang Xingshan Tian Fengzhen Zhou | 2007 | Vaccine2007，25，10： | 1 |
| 19 | Genaralizations of some identities involving the Fibonacci numbers显示文摘 | FengZhen Zhao and Tianming Wang | 2001 | The Fibonacci Quarterly2001，39，： | 1 |
| 20 | Investigation on the rheol0gical behavior of nylon 6/11 显示文摘 | Hu Guosheng Wang Biaobing Gao Fengzhen | 2006 | Materials Science & Engineering A2006，426，： | 1 |
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