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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Perinatal complications and live-birth outcomes following assisted reproductive technology: a retrospective cohort study显示文摘Background:With the development of assisted reproductive technology(ART)and its increasing success rate in the mainland of China,more attention has been paid to the safety of ART.In this study,we explored the associations between conception by ART and pregnancy/perinatal complications,and neonatal outcomes compared with similar outcomes following spontaneous conception.Methods:This retrospective cohort study of pregnancies over a 3-year period(2013-2015)was performed at Beijing Obstetrics and Gynecology Hospital,Beijing,China.Subjects were divided into two groups:conception by ART(n=2256)or spontaneous conception(n=6768).According to different fertilization modes,the ART group was divided into in vitro fertilization(IVF,n=1873)and intracytoplasmic sperm injection(ICSI,n=383)subgroups.The ART group was also divided into two different embryo transfer methods;fresh embryo transfer(ET,n=1583)and frozen embryo transfer(FET,n=673)subgroups.Pregnancy complications,perinatal complications,and neonatal outcomes of the enrolled subjects were investigated and analyzed by univariate analysis and multivariate logistic regression.Results:After adjustment for maternal age,gravidity,parity,maternal education,smoking,alcohol consumption,and body mass index(BMI),pregnancies conceived by ART were associated with a significantly increased incidence of gestational diabetes mellitus(GDM;OR 1.88,95%CI 1.56-2.27),gestational hypertension(OR 2.18,95%CI 1.83-2.60),and intrahepatic cholestasis of pregnancy(ICP)(OR 2.79,95%CI 2.15-3.64),compared with spontaneous conception.These associations were similar for the singleton group.In the twin group,only the incidence of ICP was significantly higher than in controls.We found that pregnancies conceived by ART were associated with perinatal complications,including placental abruption(OR 2.14,95%CI 1.33-3.45),premature rupture of membranes(PROM;OR 1.24,95%CI 1.06-1.45),postpartum hemorrhage(OR 2.89,95%CI 2.33-3.59)and polyhydramnios(OR 2.01,95%CI 1.29-3.16).The singleton group had a similar result with placental abruption,but not with fetal membranes ruptures before labor and polyhydramnios.There were no significant differences in the incidence of these perinatal complications in the twin group.Some neonatal outcomes,including preterm labor(OR 4.29,95%CI 3.84-4.80)and low birth weight(OR 1.72,95%CI 1.42-2.08),were more likely to occur with singleton births after ART.However,there were no significant differences for these outcomes from twin pregnancies.Perinatal complications and neonatal outcomes were consistent between the IVF and ICSI subgroups.The FET and ET subgroups showed a similar increase in complications,except for the incidence of placental abruption.After taking into account the effects of parity,birth plurality and maternal age,the ART group still exhibited increased maternal and neonatal complications,although some differences narrowed or disappeared.Conclusions:This retrospective cohort study demonstrated that patients who underwent ART were at increased risk of several adverse pregnancy outcomes compared with women who conceived spontaneously.These complications may be attributed in part to the relatively high multiple pregnancy rate after ART.Elective single embryo transfer should be promoted in China to reduce the obstetrical risks of ART pregnancy.Singletons of ART pregnancy exhibited increased maternal and neonatal complications as well,suggesting that underlying infertility or other maternal or parental factors may contribute to the adverse outcomes. | Ling-Ling Lei Yong-Lian Lan Shu-Yu Wang Wei Feng Zhi-Jin Zhai | 2019 | Chinese Medical Journal2019，，20： | 24 |
| 3 | An exact solution of unsteady Couette flow of generalized second grade fluid显示文摘The fractional calculus approach is introduced into the rheological constitutive model of a generalized second grade fluid. A constitutive model with fractional derivative is developed for the generalized second grade fluid. Unsteady Couette flow of the generalized second grade fluid is studied by using the method of the discrete inverse Laplace transform and generalized Mittag-Leffler function. And then an exact solution is obtained for this problem with arbitrary fractional derivative. This provides a new analytical tool for the study of viscoelastic fluid mechanics. | TAN Wenchang XIAN Feng WEI Lan | 2002 | Chinese Science Bulletin2002，47，21： | 17 |
| 4 | Value of superb micro-vascular imaging in predicting ischemic stroke in patients with carotid atherosclerotic plaques显示文摘BACKGROUND Unstable carotid atherosclerotic plaques are prone to cause ischemic stroke.Contrast-enhanced ultrasound(CEUS) is the primary method of assessing plaque stability, but CEUS cannot be a method for screening for unstable plaque. The emergence of superb micro-vascular imaging(SMI) offers the possibility of clinically screening for unstable plaque AIM To investigate the value of SMI in predicting ischemic stroke in patients with carotid atherosclerotic plaques.METHODS Patients with carotid atherosclerotic plaques(luminal stenosis of 50%-70%) were enrolled into the present study. All patients received conservative medication.The patient's clinical baseline data, serological data, CEUS and SMI data were analyzed. All patients underwent a 3-year follow-up. The follow-up endpoint was the occurrence of ischemic stroke and patients were divided into stroke group and non-stroke group according to whether the prognosis occurred or not.Subsequently, the difference in clinical data was compared, the correlation of SMI and CEUS was analyzed, and multiple Cox regression and receiver operating characteristic curve were applied to investigate the value of SMI and CEUS in predicting cerebral arterial thrombosis in three years.RESULTS In this study, 43 patients were enrolled in the stroke group and 82 patients were enrolled in the non-stroke group. Cox regression revealed that SMI level(P =0.013) and enhancement intensity(P = 0.032) were the independent factors influencing ischemic stroke. There was a positive correlation between SMI level and enhancement intensity(r = 0.737, P = 0.000). The area under curve of SMI level predicting ischemic stroke was 0.878. The best diagnostic point was ≥ level Ⅱ, and its sensitivity and specificity was 86.05% and 79.27%. The area under curve of enhancement intensity predicting ischemic stroke was 0.890. The best diagnostic point was 9.92 db, and its sensitivity and specificity was 88.37% and89.02%. As the SMI level gradually increased, the incidence of ischemic stroke increased gradually(X^2 = 108.931, P = 0.000).CONCLUSION SMI can be used as a non-invasive method of screening for unstable plaques and may help prevent ischemic stroke. | De-Bin Yang Jie Zhou Lan Feng Rong Xu Ying-Chun Wang | 2019 | World Journal of Clinical Cases2019，7，7： | 16 |
| 5 | Holocene environmental change and archaeology,Yangtze River Valley,China:Review and prospects显示文摘Holocene 环境变化和环境考古学是学习人地球的相互作用系统的一个国际工程的重要部件。这篇论文在最后三十年在长江山谷考察 Holocene 环境变化和环境考古学研究的进步,那包括大淡水湖, Holocene 违反和海平面的变化的进化, Holocene 气候变化和东方亚洲人季风变化,在原始文明和环境变化,文化打断和 palaeoflood 事件的上升和秋天之间的关系,以及在 agri 的起源之间的关系这些研究部件是由标明日期建立地区性的环境、文化的进化的一张年表的湖的沉积,石笋和泥炭的 underpinned。学科交差并且另外的环境代理指示物需要在长江山谷的上面、中间、更低的活动范围在考古学的地点形成和自然沉积环境的比较研究被过去常。现代技术象遥感,分子的 bioarchaeology ,和虚拟现实那样,应该综合与当前过去常标明日期,在环境考古学macro-studies,的分析的 geochemical , sedimentological ,和 palaeobotanical 方法并且微学习,以便在长江山谷区域提供更大的全面卓见进 Holocene 环境、文化的相互作用和变化。 | Li Wu Feng Li Cheng Zhu Lan Li Bing Li | 2012 | Geoscience Frontiers2012，3，6： | 16 |
| 6 | Clinical Characteristics, Radiological Features and Gene Mutation in 10 Chinese Families with Spinocerebellar Ataxias显示文摘 | Jian-Wen Chen Li Zhao Feng Zhang Lan Li Yu-Hang Gu Jing-Yuan Zhou Hui Zhang Ming Meng Kai-Hua Zhang Wei-Dong Le Chun-Bo Dong | 2015 | Chinese Medical Journal2015，，13： | 14 |
| 7 | Sequential use of transarterial chemoembolization and percutaneous cryosurgery for hepatocellular carcinoma显示文摘AIM: To evaluate the efficacy of sequential use of transarterial chemoembolization (TACE) and percutaneous cryosurgery for unresectable hepatocellular carcinoma (HCC). METHODS: Four hundred and twenty patients were enrolled in this study. The patients, who were considered to have unresectable tumors due to their location or size or comorbidity, were divided into sequential TACE-cryosurgery (sequential) group (n = 290) and cryosurgery alone (cryoalone) group (n = 130). Patients in the sequential group tended to have larger tumors and a greater number of tumors than those in the cryo-alone group. Tumors larger than 10 cm in diameter were only seen in the sequential group. TACE was performed with the routine technique and percutaneous cryosurgery was conducted under the guidance of ultrasound 2-4 wk after TACE. RESULTS: During a mean follow-up period of 42 ± 17 mo (range, 24-70 mo), the local recurrence rateat the ablated area was 17% for all patients, 11% and 23% for patients in sequential group and cryoalone groups, respectively (P = 0.001). The overall 1-, 2-, 3-, 4and 5-year survival rate was 72%, 57%, 47%, 39% and 31%, respectively. The 1and 2-year survival rates (71% and 61%) in sequential group were similar to those (73% and 54%) in cryo-alone group (P = 0.69 and 0.147), while the 4and 5-year survival rates were 49% and 39% in sequential group, higher than those (29% and 23%) in cryo-alone group (P = 0.001). Eighteen patients with large HCC (> 5 cm in diameter) survived for more than 5 years after sequential TACE while no patient with large HCC (> 5 cm in diameter) survived more than 5 years after cryosurgery. The overall complication rate was 24%, and the complication rates were 21% and 26% for the sequential and cryo-alone groups, respectively (P = 0.06). The incidence of hepatic bleeding was higher in cryo-alone group than in sequential group (P = 0.02). Liver crack only occurred in two patients of the cryoalone group. CONCLUSION: Pre-cryosurgical TACE can increase the cryoablation efficacy and decrease its adverse effects, especially bleeding. Sequential TACE and cryosurgery may be the better procedure for unresectable HCC, especially for large HCC. | Ke-Cheng Xu Li-Zhi Niu Qiang Zhou Yi-Ze Hu De-Hong Guo Zheng-Ping Liu Bing Lan Feng Mu Ying-Fei Li Jian-Sheng Zuo | 2009 | World Journal of Gastroenterology2009，15，29： | 13 |
| 8 | Gene expression signatures for phlegm-dampness constitution of Chinese Medicine显示文摘Dear Editor,The term'individualized medicine'was first used in Biochemistry Individuality in 1956 by Williams(Williams et al.,1956).With the emergence of the post-genome era in the21st century,individualized precision medicine has attracted increasing attention.In traditional Chinese medicine(TCM),there is also concept of individualized medicine,which is known as the Chinese constitutional theory,proposed in the1970s(Wang,2012).According to this theory,the | Lingru Li Juan Feng Haiqiang Yao Lan Xie Yu Chen Lingling Yang Shujuan Hou Shipeng Zhao Ranran Sun Yanling Wu Tongtong Bai Yingshuai Li Ruoxi Yu Ji Wang Qi Wang | 2017 | Science China(Life Sciences)2017，60，1： | 13 |
| 9 | Exploring coupling coordination between urbanization and ecosystem quality (1985-2010): a case study from Lianyungang City, China显示文摘都市化过程在生态的功能和风景模式通过改变影响生态系统。当前,全部的生态系统服务价值(ESV ) 的分析指向了人从地区性的生态系统获得,但是通常不关于生态的结构和模式包括信息的全面好处。因此,结果不能反映本地生态系统的全面状态。我们为评估地区性的生态系统的质量基于 ESVand 风景度量标准建议新、综合生态系统质量指示物。我们为从 1985 ~ 2010 在 Lianyungang 城市里评估在都市化和生态系统质量之间的相互关系采用了协作的 a coupled 度的方法。在都市化和生态系统质量之间的协作的联合的度显示出一条反的 U 字形的曲线。在都市化(19851995 ) 的主要阶段,都市化和生态系统联合的度恰好就被平衡。从 1995 直到 2000,联合系统到达了一个平衡条件,都市化水平在增加了。自从 2000,都市化过程加速了。在都市化和生态系统之间的协作在 2005 完成了最佳条件。一个转弯的点同时出现了,并且联合协作的度开始从最佳掉落。随后,联合系统再次进入了一个恰好平衡的州。总的来说,自从 1985,生态系统质量的全面水平减少了并且在 2005 以后严厉地降级了,建议本地生态系统质量的全面降级。 | Junyong AI Lan FENG Xiaowei DONG Xiaodong ZHU Yangfan LI | 2016 | Frontiers of Earth Science2016，10，3： | 13 |
| 10 | Management of granulomatous lobular mastitis: an international multidisciplinary consensus(2021 edition)显示文摘Granulomatous lobular mastitis(GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the evidence.Levels of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions,etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and pathology.The approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidencebased consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM. | Qian-Qian Yuan Shu-Yuan Xiao Omar Farouk Yu-Tang Du Fereshte Sheybani Qing Ting Tan Sami Akbulut Kenan Cetin Afsaneh Alikhassi Rami Jalal Yaghan Irmak Durur-Subasi Fatih Altintoprak Tae Ik Eom Fatih Alper Mustafa Hasbahceci David Martínez-Ramos Pelin Seher Oztekin Ava Kwong Cedric W.Pluguez-Turul Kirstyn EBrownson Shirish Chandanwale Mehran Habib Liu-Yi Lan Rui Zhou Xian-Tao Zeng Jiao Bai Jun-Wen Bai Qiong-Rong Chen Xing Chen Xiao-Ming Zha Wen-Jie Dai Zhi-Jun Dai Qin-Yu Feng Qing-Jun Gao Run-Fang Gao Bao-San Han Jin-Xuan Hou Wei Hou Hai-Ying Liao Hong Luo Zheng-Ren Liu Jing-Hua Lu Bin Luo Xiao-Peng Ma Jun Qian Jian-Yong Qin Wei Wei Gang Wei Li-Ying Xu Hui-Chao Xue Hua-Wei Yang Wei-Ge Yang Chao-Jie Zhang Fan Zhang Guan-Xin Zhang Shao-Kun Zhang Shu-Qun Zhang Ye-Qiang Zhang Yue-Peng Zhang Sheng-Chu Zhang Dai-Wei Zhao Xiang-Min Zheng Le-Wei Zheng Gao-Ran Xu Wen-Bo Zhou Gao-Song Wu | 2022 | Military Medical Research2022，9，4： | 13 |
| 11 | Methyl Jasmonate Reduces Chilling Injury and Maintains Postharvest Quality in Peaches显示文摘Zhaohui peaches (Prunus persica Batsch) were treated with 0 (CK), 1, 10 or 100 μmol L-1methyl jasmonate (MeJA) vapor at 20℃ for 24 h before stored at 0℃ for 35 d. The untreated fruits showed chilling injury (CI) symptoms after 4 wk of storage, as indicated by increased fruit firmness and reduced extractable juice, which is referred as leatheriness. Treatment with 1 and 10 μmol L-1 MeJA promoted normal ripening and softening, maintained higher levels of extractable juice, titratable acidity, pectinesterase (PE)and polygalacturonase (PG) activities, inhibited increases in fruit fresh weight loss, decay incidence, electrolyte leakage and MDA content, and improved color development, thereby preventing chilling injury symptoms development and maintaining edible quality. MeJA treatment also delayed the climacteric rise in respiratory rate, but promoted ethylene production during the later period of cold storage, suggesting that ethylene may involve in CI development of peaches. These results indicate that 1 and 10 μmol L-1 MeJA treatments could be used to reduce CI development and decay incidence in peaches. | FENG Lei, ZHENG Yong-hua, ZHANG Yan-fen, WANG Feng ZHANG Lan and LU Zhao-xin(College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 , P.R.China) | 2003 | Agricultural Sciences in China2003，2，11： | 13 |
| 12 | Target classification with low-resolution radar based on dispersion situations of eigenvalue spectra显示文摘Most low-resolution radar systems, especially ground surveillance radar systems, work at relatively low pulse repeat frequency (PRF) and with short time-on-target (TOT) (duration in scanning). Low PRF leads to Doppler ambiguity and short TOT results in low Doppler resolution, which poses a problem to target classification with low-resolution radar based on the jet engine modulation (JEM) characteristic of radar echo. From the pattern classification viewpoint, we propose a method of using dispersion situations of JEM eigenvalue spectra to categorize aeroplanes into three kinds, namely turbojet aircraft, prop aircraft and helicopter. We analyze the mathematical model of JEM echoes consisting of a series of line spectra and regard them as a sum of several series of harmonious waves. Classification features can be extracted based on the harmonious wave sum model. Some schemes for extracting features from echoes within or between pulses are proposed. Low-dimensional features are extracted to reduce computation burden. Our methods do not compensate for the fuselage echoes and are insensitive to the variation of fuselage Doppler. The feasibility of our methods is demonstrated by simulation experiment. | CHEN Feng, LIU HongWei, DU Lan & BAO Zheng National Laboratory of Radar Signal Processing, Xidian University, Xi’an 710071, China | 2010 | Science China(Information Sciences)2010，53，7： | 12 |
| 13 | Mechanisms and pharmacokinetic/pharmacodynamic profiles underlying the low nephrotoxicity and ototoxicity of etimicin显示文摘Etimicin(ETM),a fourth-generation aminoglycosides(AGs),is now widely clinically used in China due to its high efficacy and low toxicity.However,the mechanisms underlying its low nephrotoxicity and ototoxicity remain unclear.In the present study we compared the antibacterial and toxicity profiles of etimicin,gentamicin(GM,a second-generation AG),and amikacin(AMK,a third-generation AG),and investigated their pharmacokinetic properties in the toxicity target organs(kidney and inner ear)and subcellular compartments.We first demonstrated that ETM exhibited superior antibacterial activities against clinical isolates to GM and AMK,and it exerted minimal nephrotoxicity and ototoxicity in rats following multi-dose administration.Then,we conducted pharmacokinetic studies in rats,showed that the three AGs accumulated in the kidney and inner ear with ETM being distributed to a lesser degree in the two toxicity target organs as compared with GM and AMK high-dose groups.Furthermore,we conducted in vitro experiments in NRK-52E rat renal tubular epithelial cells and HEI-OC1 cochlear hair cells,and revealed that all the three AGs were distributed predominantly in the mitochondria with ETM showing minimal accumulation;they not only directly inhibited the activity of mitochondrial complexesⅣandⅤbut also inhibited mitochondrial function and its related PGC-1α-NRF1-TFAM pathway;ETM caused minimal damage to the mitochondrial complex and mitochondrial biogenesis.Our results demonstrate that the minimal otonephrotoxicity of ETM results from its lesser accumulation in mitochondria of target cells and subsequently lesser inhibition of mitochondrial function.These results provide a new strategy for discovering novel AGs with high efficacy and low toxicity. | Lan Yao Jing-wei Zhang Bin Chen Ming-min Cai Dong Feng Qi-zhi Wang Xin-yu Wang Jian-guo Sun Yi-wen Zheng Guang-ji Wang Fang Zhou | 2020 | Acta Pharmacologica Sinica2020，41，6： | 10 |
| 14 | Microstructure,texture evolution and mechanical properties of cold rolled Ti-32.5Nb-6.8Zr-2.7Sn biomedical beta titanium alloy显示文摘Ti-32.5 Nb-6.8 Zr-2.7 Sn(TNZS,wt%) alloy was produced by using vacuum arc melting method,followed by solution treatment and cold rolling with the area reductions of 50% and 90%.The effects of cold rolling on the microstructure,texture evolution and mechanical properties of the experimental alloy were investigated by optical microscopy,X-ray diffraction,transmission electron microscopy and universal material testing machine.The results showed that the grains of the alloy were elongated along rolling direction and stress-induced α'' martensite was not detected in the deformed samples.The plastic deformation mechanisms of the alloy were related to {112} 111 type deformation twinning and dislocation slipping.Meanwhile,the transition from γ-fiber texture to α-fiber texture took place during cold rolling and a dominant {001} 110_(α-fiber) texture was obtained after 90% cold deformation.With the increase of cold deformation degree,the strength increased owing to the increase of microstrain,dislocation density and grain refinement,and the elastic modulus decreased owing to the increase of dislocation density as well as an enhanced intensity of {001} 110_(α-fiber)texture and a weakened intensity of {111} 112_(γ-fiber)texture.The 90% cold rolled alloy exhibited a great potential to become a new candidate for biomedical applications,since it possesses low elastic modulus(47.1 GPa),moderate strength(883 MPa) and high elastic admissible strain(1.87%),which are superior than those of Ti-6 Al-4 V alloy. | Chunbo Lan Yu Wu Lili Guo Huijuan Chen Feng Chen | 2018 | Journal of Materials Science & Technology2018，34，5： | 10 |
| 15 | Isoflavones and phytosterols contained in Xuezhikang capsules modulate cholesterol homeostasis in high-fat diet mice显示文摘目的: Xuezhikang (XZK ) ,红酵母米饭的一篇摘录,广泛地在繁体中文药被使用了到对待心血管的疾病。三部分 F1, F2 和 F3 (首先包含 isoflavones, monacolins 或 phytosterols,分别地) 从 Xuezhikang 囊被提取。在这研究,我们评估了这些部分的类脂化合物阴沉的效果并且探索了 actions.Methods 的潜在的机制:与一本高脂肪的食谱( HFD )对待的老鼠是口头上地 adminis¬ ; tered lovastatin ( 10 mg· ; kg −1·d−1), XZK ( 1200 mg· ; kg −1·d−1), F1 ( 27.5 mg· ; kg −1·d−1), F2 ( 11.3 mg· ; kg −1·d−1 类脂化合物用商业酶的工具包被测量,并且基因的 mRNA 和蛋白质层次在胆固醇包含了,胆汁酸动态平衡用 qRT-PCR 和西方的污点分析被评估, respectively.Results : XZK 增加了类脂化合物和胆汁的烘便的排泄酸,减少的浆液 TC , TG 和 LDL-C 在40%的层次,55%和46%,分别地并且在31%的增加的浆液高级数据链路控制。F1 的管理镇压了在 24% 和 52% 的浆液 TC 和 TG,分别地并且 CYP7A1 的提高的肝的合成。它也通过 upregulating BSEP 和 downregulating NTCP 在烘便的排泄增加了胆汁酸的肝的消除 79% 。 F3 的管理减少了分别地,浆液 TC , TG 和 LDL-C 在33%,29%和39%铺平并且在28%的增加的浆液高级数据链路控制, sig¬ ;由禁止 NPC1L1 的抄写的胆固醇的 nificantly 减少的肠的吸收,和在96%的 TC , FC 和 CE 的提高的排泄,72%和101%分别地。F2 的管理显示出 lovastatin.Conclusion 的类似于那些的药理学效果: 在 XZK 的 Isoflavones 和 phytosterols 通过 lovastatin 不同于那些的机制在 HFD 老鼠施加胆固醇阴沉的效果。Isoflavones 和 phytosterols 以一种赞美方式行动:通过提高胆汁酸的消除并且分别地减少肠的胆固醇吸收。 | Dong FENG Jian-guo SUN Run-bin SUN Bing-chen OU-YANG Lan YAO Ji-ye AA Fang ZHOU Jing-wei ZHANG Jian ZHANG Guang-ji WANG | 2015 | Acta Pharmacologica Sinica2015，36，12： | 9 |
| 16 | Epidemiology,clinical and laboratory characteristics of currently alive HIV-1 infected former blood donors naive to antiretroviral therapy in Anhui Province,China显示文摘Background Unregulated commercial blood/plasma collection among farmers occurred between 1992 and 1995 in central China and caused the second major epidemic of human immunodeficiency virus type 1 (HIV-1) infection in China. It is important to characterize HIV-1-infected former blood donors and to study characteristics associated with disease progression for future clinical intervention and vaccine development. Methods A cross-sectional study was performed on HIV-1-infected former blood donors (FBDs) and age-matched HIV-seronegative local residents. Demographic, epidemiologic, clinical and key laboratory data were collected from all study participants. Both unadjusted and adjusted multivariate linear regressions were employed to analyze the association of the decrease of CD4+ T-cell counts with other characteristics. Results Two hundred and ninety-four HIV-1-infected FBDs and 59 age-matched HIV-seronegative local residents were enrolled in this study. The unregulated blood/plasma collection occurred more than a decade (10.8 -12.8 years) ago, which caused the rapid spread of HIV-1 infection and the high prevalence of co-infection with hepatitis C virus (HCV, 89.5%); hepatitis B virus (HBV) co-infection was observed in only 11 HIV+participants (3.7%). Deterioration in both clinical manifestation and laboratory parameters and increase of viral loads were observed in parallel with the decrease of CD4+ T-cell counts. The decrease of total lymphocyte counts (P<0.001) and hemoglobin levels (P<0.001) and the appearance of dermatosis (P=0.03) were observed in parallel with the decrease of CD4+ T-cell counts whereas viral loads (P<0.001) and CD8+ T-cell counts (P=0.01) were inverselyassociated with CD4+ T-cell counts. Conclusions Co-infection with HCV but not HBV is highly prevalent among HIV-1-infected FBDs. CD4+ T-cell counts is a reliable indicator for disease progression among FBDs. Total lymphocyte counts, hemoglobin level and appearance of dermatosis were positively associated with CD8+ T-cell counts and viral loads were inversely associated with the decreased CD4+ T-cell counts. | XU Jian-qing WANG Jian-jun HAN Li-feng XU Chen RUAN Yu-hua XU Zhen-hou CHEN Xi LIU Zhen-dong WANG Jun SU Bing DING Xin-ping GAO Bing GU Yong-bin CAO Xiao-yun XING Hui HONG Kun-xue PENG Hong ZHAO Quan-bi YUAN Lin FENG Yi ZHANG Gui-yun MA Li-ying WU Lan SHAO Yi-ming | 2006 | Chinese Medical Journal2006，，23： | 9 |
| 17 | Simultaneous additive and subtractive three-dimensional nanofabrication using integrated two-photon polymerization and multiphoton ablation显示文摘Modern three-dimensional nanofabrication requires both additive and subtractive processes.However,both processes are largely isolated and generally regarded as incompatible with each other.In this study,we developed simultaneous additive and subtractive fabrication processes using two-photon polymerization followed by femtosecond(fs)laser multiphoton ablation.To demonstrate the new capability,submicrometer polymer fibers containing periodic holes of 500-nm diameter and microfluidic channels of 1-mm diameter were successfully fabricated.This method combining both two-photon polymerization and fs laser ablation improves the nanofabrication efficiency and enables the fabrication of complex three-dimensional micro-/nanostructures,promising for a wide range of applications in integrated optics,microfluidics and microelectromechanical systems. | Wei Xiong Yun Shen Zhou Xiang Nan He Yang Gao Masoud Mahjouri-Samani Lan Jiang Tommaso Baldacchini Yong Feng Lu | 2012 | Light(Science & Applications)2012，1，1： | 9 |
| 18 | Treatment of Donor-derived Carbapenem-resistant Klebsiella pneumoniae Infection after Renal Transplantation with Tigecycline and Extended-infusion Meropenem显示文摘Objective Donor-derived carbapenem-resistant Klebsiella pneumoniae(CRKP)infection has recently emerged as a critical early complication after renal transplantation.Although CRKP is usually sensitive to tigecycline,monotherapy with this drug is often less than effective.We investigated the efficacy of a combined regimen of tigecycline with high-dose,extended-infusion meropenem in the treatment of donor-derived CRKP infection after kidney transplantation.Methods From Jan.2016 to Dec.2017,a total of 12 CRKP isolates were detected from cultures of the organ preservation solution used for soaking the donor kidneys at our institute.Probable or possible donor-derived infection(DDI)was identified in 8 transplant recipients.Clinical data were retrospectively analyzed.Results Klebsiella pneumoniae carbapenemase-2(KPC-2)-producing CRKP was reported to be positive in organ preservation solution cultures at 3.5±0.9 days after transplantation,leading to surgical site(n=3),urinary tract(n=4),and/or bloodstream(n=2)infections in 8 recipients.The drug susceptibility tests showed that CRKP was sensitive to tigecycline,but resistant to meropenem.In 7 patients who received tigecycline combined with high-dose extended-infusion meropenem,DDIs were successfully cured.The length of hospital stay was 31(18–129)days,and the serum creatinine at discharge was 105.8±16.7µmol/L.The one remaining patient who received tigecycline combined with intravenous-drip meropenem died of septic shock.A median follow-up of 43 months(33–55)showed no recurrence of new CRKP infection in the 7 surviving recipients.Conclusion It was suggested that a prompt and appropriate combination therapy using tigecycline with high-dose extended-infusion meropenem is effective in treating donor-derived KPC-2-producing CRKP infection after renal transplantation. | Zhi-qiang WANG Zhi-liang GUO Hao FENG Cheng FU Guang-yuan ZHAO Ke MA Lan ZHU Gang CHEN | 2021 | Current Medical Science2021，41，4： | 8 |
| 19 | THE STRUCTURE OF ARMILLARIZIN,A NEW PROTOILLUDANE SESQUITERPENOID AROMATIC ESTER FROM ARMILLARIA MELLEA(VAHL.EX FR)QUEL.显示文摘A new protoilludane sesquiterpenoid aromatic ester,named armillarizin(1)has been isolated from the artificially cultured mycelium of Armillaria me-llea(Vahl.ex Fr)Quel.(Tricholomataceae).Its structure was deduced on the ba-sis of spectral analyses.The relative configuration was established from thenuclear Overhauser effects and from the values of ~1H-~1H coupling constants. | Jun Shan YANG Ya Lun SU Yu Lan WANG Xiao Zhang FENG De Quan YU Xiao Tian LIANG Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing,100050 | 1990 | Chinese Chemical Letters1990，1，2： | 8 |
| 20 | Robust radar automatic target recognition algorithm based on HRRP signature显示文摘自动目标识别(ATR ) 是为现代雷达的重要功能。目标的高分辨率范围侧面(HRRP ) 包含目标结构签名,例如目标尺寸, scatterer 分发,等等,它是为 ATR 的有希望的签名。目标 HRRP 的统计建模是为 HRRP 统计识别的关键阶段,包括模型选择和参数评价。为统计识别算法,测试样品作为训练数据的跟随一样的分发模型,这通常被假定。因为收到的 HRRP 的 signal-to-noise 比率(SNR ) 是目标距离的功能,假设不能在实践被遇见。在这份报纸,我们在场为 HRRP 统计识别的一个柔韧的方法测试 HRRP 的 SNR 什么时候比训练的低,取样。噪音是散布的假定独立 Gaussian,当 HRRP 被概率的主要部件分析(PPCA ) 建模时模型。模仿的实验基于测量数据显示出建议方法的有效性。 | Hongwei LIU Feng CHEN Lan DU Zheng BAO | 2012 | Frontiers of Electrical and Electronic Engineering in China2012，7，1： | 8 |
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