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| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 2 | Effects of pre-commercial thinning on growth and reproduction in post-fire regeneration of Pinus halepensis Mill显示文摘 | IRENE R ENCAMA R G FELIPE B | 2013 | Annals of Forest Science2013，70，4： | 1 |
| 3 | comparison of the GDX VCC scanning laser polarimeter, HRT Ⅱ confocal scanning laser opthalmoscope, and stratus OCT optical coherence tomography for the detection of glaucoma显示文摘 | Felipe AM Linda MZ Christopher B | 2011 | Arch Ophthalmol2011，122，： | 1 |
| 4 | Effects of dietary live yeast Debaryomyces hansenii on the im- mune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress 显示文摘 | Martha R B Dariel T R Felipe A V | 2008 | Aquaculture2008，280，14： | 1 |
| 5 | Tapered GRIN fiber microsensor显示文摘 | Felipe Beltr6n-Mejia Claudecir R Biazoli Cristiano M B Cordeiro | 2014 | Opt Express2014，22，30： | 1 |
| 6 | Almeida e Silva, Response surface methodology for xylital production from sugarcane bagasse hemicellulosic hydrolyzate using controlled vacuum evaporation process variables显示文摘 | Rita C L B Rodrigues Maria das Gracas A Felipe Joao B | 2003 | Process Biochemistry2003，38，： | 1 |
| 7 | Molecular characterization and evaluation of mycorrhizal capacity of SuiUus isolates from central spain for the selection of fungal inoculants 显示文摘 | Ruiz D B Rinc6n A M Felipe M R | 2006 | Mycorrhiza2006，16，7： | 1 |
| 8 | HCV RNA in peripheral blood cell subsets in HCV-HIV coinfected patients at the end of PegIFN/RBV treatment is associated with virologic relapse显示文摘 | de Felipe B Leal M Soriano-Sarabia N | | 0，，： | 1 |
| 9 | FOXP3 expression accurately defines the population of intratumoral regulatory T cells that selectively accumulate in metastatic melanoma lesions显示文摘 | Ahmadzadeh M Felipe Silva A Heemskerk B | 2008 | Blood2008，112，13： | 1 |
| 10 | Apoptosis induced by a low-carbohydrate and high-protein diet in rat livers显示文摘AIM: To determine whether high-protein, high-fat, and low-carbohydrate diets can cause lesions in rat livers.METHODS: We randomly divided 20 female Wistar rats into a control diet group and an experimental diet group. Animals in the control group received an AIN-93 M diet, and animals in the experimental group received an Atkins-based diet(59.46% protein, 31.77% fat, and 8.77% carbohydrate). After 8 wk, the rats were anesthetized and exsanguinated for transaminases analysis, and their livers were removed for flow cytometry, immunohistochemistry, and light microscopy studies. We expressed the data as mean ± standard deviation(sd) assuming unpaired and parametric data; we analyzed differences using the student's t-test. statistical significance was set at P < 0.05.RESULTS: We found that plasma alanine aminotransferase and aspartate aminotransferase levels were significantly higher in the experimental group than in the control group. According to flow cytometry, the percentages of nonviable cells were 11.67% ± 1.12% for early apoptosis, 12.07% ± 1.11% for late apoptosis, and 7.11% ± 0.44% for non-apoptotic death in the experimental diet group and 3.73% ± 0.50% for early apoptosis, 5.67% ± 0.72% for late apoptosis, and 3.82% ± 0.28% for non-apoptotic death in the control diet group. The mean percentage of early apoptosis was higher in the experimental diet group than in the control diet group. Immunohistochemistry for autophagy was negative in both groups. sinusoidal dilation around the central vein and small hepatocytes was only observed in the experimental diet group, and fibrosis was not identified by hematoxylin-eosin or Trichrome Masson staining in either group.CONCLUSION: Eight weeks of an experimental diet resulted in cellular and histopathological lesions in rat livers. Apoptosis was our principal finding; elevated plasma transaminases demonstrate hepatic lesions. | Maria Emília L Monteiro Analucia R Xavier Felipe L Oliveira Porphirio JS Filho Vilma B Azeredo | 2016 | World Journal of Gastroenterology2016，22，22： | 1 |
| 11 | Batch xylitol production from wheat straw hemicellulosic hydrolysate using Candida guilliermondii in a stirred tank reactor显示文摘 | Larissa Canilha Joo B Almeida e Silva Maria G A Felipe | 2003 | Biotechnology Letters2003，25，： | 1 |
| 12 | Fault diagnosis scheme for opencircuit faults in field-oriented control induction motor drives显示文摘 | Diego Rivelino Espinoza-Trejo Daniel Ulises Campos-Delgado Guillermo Bossio Ernesto Bárcenas JoséEnrique HernándezDíez Luis Felipe Lugo-Cordero | 2013 | IET Power Electroncis2013，6，5： | 1 |
| 13 | A phase ii randomized study e valuating the addition of iniparib to gemcitabine plus cisplatin as first-line therapy for metastatic non-small cell lung cancer显示文摘 | Novello S Besse B Felip E | 2014 | Ann Oncol2014，25，11： | 1 |
| 14 | Batch xylitol production by Candida guilliermondii FTI20037from sugarcane bagasse hemicellulosic hydrolyzate at controlled pH values显示文摘 | R C L B Rodrigues M G A Felipe I C Roberto | 2003 | Bioprocess Biosyst Eng2003，26，： | 1 |
| 15 | 2012, Generation of virtual models of cultural heritage 显示文摘 | Amparo N Felipe B Joaquin R | 2012 | J Cult Herit2012，13，： | 1 |
| 16 | Re- sponse surface methodology for xylitol production from sugarcane bagasse hemicellulosic hydrolyzate using controlled vacuum evaporation process variables显示文摘 | Rodrigues R C L B Felipe M G A Silva J B A | 2003 | Process Biochem- istry2003，38，8： | 1 |
| 17 | Retinol sup- plementation induces oxidative stress and modulates antioxidant enzyme activities in rat sertoli cells 显示文摘 | FELIPE D FABIO K MARA S B | 2001 | Free Radical Research2001，34，4： | 1 |
| 18 | Inhibition of cell proliferation and migration by oxidative stress from ascorbate-driven juglone redox cycling in hu- man bladder-derived T24 cells 显示文摘 | KVIECINSKI M R PEDROSA R C FELIPE K B | 2012 | Biochemical and Bio- physical Research Communications2012，421，2： | 1 |
| 19 | Supporting Local Adaptation:The Contribution of Socio-environ- mental Fragmentation to Urban Vulnerability显示文摘 | Kerstin K Felipe L Juliane W Jordan H Katrin B Felipe I | 2014 | Applied Geography2014，55，12： | 1 |
| 20 | Long-termeffect of microbial abundance and humus-associatedenzyme activities under semiarid conditions显示文摘 | Felipe B Ellen K Teresa H Carlos García | 2008 | MedSciEntry for Microbial Ecology2008，55，65： | 1 |
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