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| 1 | Inhibiting collagen Ⅰ production and tumor cell colonization in the lung via miR-29a-3p loading of exosome-/liposome-based nanovesicles显示文摘The lung is one of the most common sites for cancer metastasis.Collagens in the lung provide a permissive microenvironment that supports the colonization and outgrowth of disseminated tumor cells.Therefore,down-regulating the production of collagens may contribute to the inhibition of lung metastasis.It has been suggested that mi R-29 exhibits effective anti-fibrotic activity by negatively regulating the expression of collagens.Indeed,our clinical lung tumor data shows that mi R-29 a-3 p expression negatively correlates with collagen I expression in lung tumors and positively correlates with patients’outcomes.However,suitable carriers need to be selected to deliver this therapeutic mi RNA to the lungs.In this study,we found that the chemotherapy drug cisplatin facilitated mi R-29 a-3 p accumulation in the exosomes of lung tumor cells,and this type of exosomes exhibited a specific lung-targeting effect and promising collagen down-regulation.To scale up the preparation and simplify the delivery system,we designed a lung-targeting liposomal nanovesicle(by adjusting the molar ratio of DOTAP/cholesterol-mi RNAs to 4:1)to carry mi R-29 a-3 p and mimic the exosomes.This liposomal nanovesicle delivery system significantly down-regulated collagen I secretion by lung fibroblasts in vivo,thus alleviating the establishment of a pro-metastatic environment for circulating lung tumor cells. | Yan Yan Cancan Du Xixi Duan Xiaohan Yao Jiajia Wan Ziming Jiang Zhongyu Qin Wenqing Li Longze Pan Zhuoyu Gu Fazhan Wang Ming Wang Zhihai Qin | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 7 |
| 2 | Optimizing ultrasensitive single electron magnetometer based on nitrogen-vacancy center in diamond显示文摘The measurement of the weak magnetic field in nanoscale resolution and at room temperature is always a significant topic in biological, physical, and material science. Such detection can be used to decide the characterization of the samples, such as cells, materials, and so on. Nitrogen-vacancy (NV) center in diamond has been proved to be able to detect a magnetic field with nano Tesla sensitivity and nanometer resolution at room temperature. Here we experimentally demonstrate an optimized NV center based single electron magnetometer in a commercial diamond and under a home-built optically detected magnetic resonance (ODMR) microscope. With current technology, we change the optically detected time window to get a better signal to noise ratio, and use dynamical decoupling to increase the slope of magnetic field amplitude versus fluorescence signal. By employing the 8-pulse XY-4 dynamical decoupling sequence we achieve a sensitivity of 18.9 nT (Hz)(1/2) , which is 1.7 times better than spin echo. We also propose a NV center based scanning diamond microscope for electron and nuclear spins detection as well as nanoscale magnetic resonance imaging. If it is realized, the NV center based magnetometry will have wide application in the future. | WANG PengFei JU ChenYong SHI FaZhan DU JiangFeng | 2013 | Chinese Science Bulletin2013，58，24： | 5 |
| 3 | Core-shell lipid-polymer nanoparticles as a promising ocular drug delivery system to treat glaucoma显示文摘Nowadays,tremendous researches have been focused on the core-shell lipid-polymer nanoparticles(LPNs) due to the advantages of both liposomes and polymer nanoparticles.In this work,LPNs were applied to encapsulate brinzolamide(Brz-LPNs) for achieving sustained drug release,improving drug corneal permeation and enhancing drug topical therapeutic effect.The structure of Brz-LPNs was composed of poly(lactic-co-glycolic) acid(PLGA) nanocore which encapsulated Brz(Brz-NPs) and lipid shell around the core.Brz-LPNs were prepared by a modified thin-film dispersion method.With the parameters optimization of Brz-LPNs,optimal Brz-LPNs showed an average particle size of151.23±1.64 nm with a high encapsulation efficiency(EE) of 86.7%±2.28%.The core-shell structure of Brz-LPNs were confirmed by transmission electronic microscopy(TEM).Fourier transformed infrared spectra(FTIR) analysis proved that Brz was successfully entrapped into Brz-LPNs.Brz-LPNs exhibited obvious sustained release of Brz,compared with AZOPT^■ and Brz-LPs.Furthermore,the corneal accumulative permeability of Brz-LPNs significantly increased compared to the commercial available formulation(AZOPT^■) in vitro.Moreover,Brz-LPNs(1 mg/mL Brz) showed a more sustained and effective intraocular pressure(IOP) reduction than Brz-LPs(1 mg/mL) and AZOPT^■(10 mg/mL Brz) in vivo.In conclusion,Brz-LPNs,as promising ocular drug delivery systems,are well worth developing in the future for glaucoma treatment. | Yang Zhou Aiping Fang Fazhan Wang Huili Li Quansheng Jin Lingjing Huang Chunmei Fu Jun Zeng Zhaohui Jin Xiangrong Song | 2020 | Chinese Chemical Letters2020，31，2： | 3 |
| 4 | Semi-elastic core-shell nanoparticles enhanced the oral bioavailability of peptide drugs显示文摘The rigidity of nanoparticles was newly reported to influence their oral delivery.Semi-elastic nanoparticles can enhance the penetration in mucus and uptake by epithelial cells.However,it is still challenging and unclear that the semi-elastic core-shell nanoparticles can enhance the oral bioavailability of peptide drugs.This study was for the first time to validate the semi-elastic coreshell poly(lactic-co-glycolic acid)(PLGA)-lipid nanoparticles(LNPs)as the carrier of the oral peptide drug.The antihypertensive peptide Val-Leu-Pro-Val-Pro(VP5)loaded LNPs(VP5-LNPs)were prepared by a modified thin-film ultrasonic dispersion method.Uptake experiment was performed in Caco-2 and HT-29 cells and monito red by high content screening(HCS)and flow cyto metric(FCM).Pharmacokinetics of VP5-LNPs was carried out in Sprague-Dawley(SD)rats and analyzed by DAS 2.0.The optimal VP5-LNPs had an average particle size of 247.3±3.8 nm,zeta potential of-6.57±0.45 mV and excellent entrapment efficiency(EE)of 89.88%±1.23%.Transmission electron microscope(TEM)and Differential scanning calorimeter(DSC)further confirmed the core-shell structure.VP5-LNPs could increase the cellular uptake in vitro and have a 2.55-fold increase in AUC0-72 h,indicating a great promotion of the o ral bioavailability.The semi-elastic LNPs remarkably improved the oral availability of peptide and could be a promising oral peptide delivery system for peptide drugs in the future. | Shengnan Zhao Jinhua Li Fazhan Wang Ting Yu Yang Zhou Lili He Yi Zhang jian Yang | 2020 | Chinese Chemical Letters2020，31，5： | 2 |
| 5 | Research on knowledge acquisition method about the IF/THEN rules based on rough set theory显示文摘The basic principles of IF/THEN rules in rough set theory are analyzed first, and then the automatic process of knowledge acquisition is given. The numerical data is qualitatively processed by the classification of membership functions and membership degrees to get the normative decision table. The regular method of relations and the reduction algorithm of attributes are studied. The reduced relations are presented by the multi-represent-value method and its algorithm is offered. The whole knowledge acquisition process has high degree of automation and the extracted knowledge is true and reliable. | Liu Daohua Yuan Sicong Zhang Xiaolong Wang Fazhan | 2008 | Journal of Systems Engineering and Electronics2008，19，3： | 2 |
| 6 | A Thermionic Tungsten Cathode Activated With Nanothoria and Prepared by Swaging Method显示文摘 | Wang Fazhan Zhang Hui Ding Bingjun | 2002 | Materials Science and Engineering A2002，336，1： | 1 |
| 7 | Cathode spots on the surface of nanostructured W alloys 显示文摘 | Su Yafeng Chen Wenge Wang Fazhan | 2005 | Materials Letters2005，，59： | 1 |
| 8 | Faults diagnosis of rolling element bearings based on modified morphological method显示文摘 | Yabin Dong Mingfu Liao Xiaolong Zhang Fazhan Wang | 2010 | Mechanical Systems and Signal Processing2010，，4： | 1 |
| 9 | A thermionic tungsten cathode activated with nanothoria and prepared by swaging method显示文摘 | Wang Fazhan Zhang Hui Ding Bingjun | 2002 | Material Science and Engineer2002，336，： | 1 |
| 10 | Identification and characterization of college students with attenu-ated psychosis syndrome in China 显示文摘 | Chen Fazhan Wang Lu Heeramun-Aubeeluck Anisha | 2014 | Psychiatry Research2014，14，3： | 1 |
| 11 | Observation of non-Markovianity at room temperature by prolonging entanglement in solids显示文摘Open quantum systems are always exposed to an external environment,which result in interacting and exchanging information between quantum systems and their surroundings.The dynamics of real open quantum systems are often expected to deviate from the idealized Markovian process of losing information to their surrounding environment and to exhibit non-Markovian | Shijie Peng Xiangkun Xu Kebiao Xu Pu Huang Pengfei Wang Xi Kong Xing Rong Fazhan Shi Changkui Duan Jiangfeng Du | 2018 | Science Bulletin2018，63，6： | 1 |
| 12 | Starch phosphorylase 2 is essential for cellular carbohydrate partitioning in maize显示文摘Carbohydrate partitioning is essential for plant growth and development,and its hindrance will result in excess accumulation of carbohydrates in source tissues.Most of the related mutants in maize(Zea mays L.)display impaired whole-plant sucrose transport,but other mechanisms affecting carbohydrate partitioning have seldom been reported.Here,we characterized chlorotic leaf3(chl3),a recessive mutation causing leaf chlorosis with starch accumulation excessively in bundle sheath chloroplasts,suggesting that chl3 is defective in carbohydrate partitioning.Positional cloning revealed that the chl3 phenotype results from a frameshift mutation in ZmPHOH,which encodes starch phosphorylase 2.Two mutants in ZmPHOH exhibited the same phenotype as chl3,and both alleles failed to complement the chl3 mutant phenotype in an allelism test.Inactivation of Zm PHOH in chl3 leaves reduced the efficiency of transitory starch conversion,resulting in increased leaf starch contents and altered carbohydrate metabolism patterns.RNA-seq revealed the transcriptional downregulation of genes related to photosynthesis and carbohydrate metabolism in chl3 leaves compared to the wild type.Our results demonstrate that transitory starch remobilization is very important for cellular carbohydrate partitioning in maize,in which Zm PHOH plays an indispensable role. | Yao Qin Ziyi Xiao Hailiang Zhao Jing Wang Yuanru Wang Fazhan Qiu | 2022 | Journal of Integrative Plant Biology2022，64，9： | 1 |
| 13 | Sub-nanotesla sensitivity at the nanoscale with a single spin显示文摘High-sensitivity detection of the microscopic magnetic field is essential in many fields.Good sensitivity and high spatial resolution are mutually contradictory in measurement,which is quantified by the energy resolution limit.Here we report that a sensitivity of 0.5 nT/(Hz)(1/2)at the nanoscale is achieved experimentally by using nitrogen-vacancy defects in diamond with depths of tens of nanometers.The achieved sensitivity is substantially enhanced by integrating with multiple quantum techniques,including real-time-feedback initialization,dynamical decoupling with shaped pulses and repetitive readout via quantum logic.Our magnetic sensors will shed new light on searching new physics beyond the standard model,investigating microscopic magnetic phenomena in condensed matters,and detection of life activities at the sub-cellular scale. | Zhiyuan Zhao Xiangyu Ye Shaoyi Xu Pei Yu Zhiping Yang Xi Kong Ya Wang Tianyu Xie Fazhan Shi Jiangfeng Du | 2023 | National Science Review2023，10，12： | 0 |
| 14 | Natural long-chain saturated fatty acids doped LNPs enabling spleen selective mRNA translation and potent cancer immunotherapy显示文摘Rationally tailored lipid nanoparticles(LNPs)with efficient and tunable delivery of mRNA in vivo are crucial for mRNA vaccines.Selective expression of antigenic protein in lymphoid tissues/organs could improve the immunostimulatory efficacy and safety of LNPs-based mRNA vaccines.Inspired by the metabolic behavior that long-chain saturated fatty acids tending to enter lymphoid tissue rather than the liver,we developed fatty acid-doped LNPs capable of mediating differential protein expressions in the liver and spleen when administered intravenously.When the molar ratio of saturated fatty acid located 60%–70%,the doped LNPs achieved the spleen selective mRNA translation.The mechanism could be attributed to the different cellular uptake behaviors of saturated fatty acids in hepatocytes.Immunization with a model antigen(ovalbumin)mRNA-loaded spleen selective LNPs,we observed enhanced antigen-specific T cell immune responses,and potent immunotherapeutic and immunoprophylactic efficacy in the mouse lymphoma model.Our natural long-chain saturated fatty acids metabolic characteristics-inspired design of LNPs for spleen-selective mRNA vaccines delivery will provide references for designing mRNA vaccines with high efficacy and safety for tumor immunotherapy. | Fazhan Wang Meng Zhang Meiling Tian Jia Lou Longze Pan Xiaoke Gao Lijing Zhang Xiaohan Lou Linyu Zhu Yuqiao Sheng Ming Wang Rui Xue Wenjing Deng Shuai Shao Zhihai Qin | 2024 | Nano Research2024，17，3： | 0 |
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