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| 1 | A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum显示文摘Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained. | ZHU Jun, WANG FangJun, DONG XiaoLi, YE MingLiang & ZOU HanFa Key Laboratory of Separation Sciences for Analytical Chemistry National Chromatographic Research and Analysis Center Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China | 2010 | Science China Chemistry2010，53，4： | 3 |
| 2 | Cryo-EM structures of human pannexin 1 channel显示文摘Dear Editor,Pannexin 1(PANX1)channel mediates adenosine-5’-triphosphate(ATP)release for purinergic signaling,1 playing important roles in multiple physiological and pathological processes including apoptotic clearance,2 inflammatory response,3 cancer progression,and metastasis.4 Diverse stimuli have been reported to activate PANX1 channel,such as voltage,membrane stretch,hypotonicity,elevated extracellular K+or intracellular Ca2+concentration,C-terminal cleavage and phosphorylation.1,5 It is perplexing that PANX1 has been demonstrated to exhibit different unitary channel properties depending on the mode of activation.1,5 Particularly,PANX1 undergoes a stepwise,quantized activation progress upon sequentially cleaving the C-terminal of each subunit. | Qiuheng Jin Bo Zhang Xiang Zheng Ningning Li Lingyi Xu Yuan Xie Fangjun Song Eijaz Ahmed Bhat Yuan Chen Ning Gao Jiangtao Guo Xiaokang Zhang Sheng Ye | 2020 | Cell Research2020，30，5： | 2 |
| 3 | Boosting the Electrochemical Performance of Li-and Mn-Rich Cathodes by a Three-in-One Strategy显示文摘There are plenty of issues need to be solved before the practi-cal application of Li-and Mn-rich cathodes,including the detrimental voltage decay and mediocre rate capability,etc.Element doping can e ectively solve the above problems,but cause the loss of capacity.The introduction of appropriate defects can compensate the capacity loss;however,it will lead to structural mismatch and stress accumulation.Herein,a three-in-one method that combines cation–polyanion co-doping,defect construction,and stress engineering is pro-posed.The co-doped Na^(+)/SO_(4)^(2-)can stabilize the layer framework and enhance the capacity and voltage stability.The induced defects would activate more reac-tion sites and promote the electrochemical performance.Meanwhile,the unique alternately distributed defect bands and crystal bands structure can alleviate the stress accumulation caused by changes of cell parameters upon cycling.Consequently,the modified sample retains a capacity of 273 mAh g^(-1)with a high-capacity retention of 94.1%after 100 cycles at 0.2 C,and 152 mAh g^(-1)after 1000 cycles at 2 C,the corresponding voltage attenuation is less than 0.907 mV per cycle. | Wei He Fangjun Ye Jie Lin Qian Wang Qingshui Xie Fei Pei Chenying Zhang Pengfei Liu Xiuwan Li Laisen Wang Baihua Qu Dong-Liang Peng | 2021 | Nano-Micro Letters2021，13，12： | 1 |
| 4 | Elucidating the Molecular Mechanism of Dynamic Photodamage of Photosystem II Membrane Protein Complex by Integrated Proteomics Strategy显示文摘Photosystem II(PSII),as a multiple-subunit chloroplast membrane-associated pigment-protein complex on the thylakoid membrane,is a primary target of light-induced photodamage.However,the overall molecular details of the conformation and composition dynamics of PSII photodamage are still controversial.In this study,we investigated systematically the dynamic conformation,degradation,and oxidation processes of PSII photodamage by integrating chemical cross-linking and top-down proteomics strategies. | Ye Zhou Zheyi Liu Mingdong Yao Jun Chen Yanan Xiao Guangye Han Jian-Ren Shen Fangjun Wang | 2022 | CCS Chemistry2022，4，1： | 1 |
| 5 | Preparation of monodisperse immobilized Ti 4+ affinity chromatography microspheres for specific enrichment of phosphopeptides显示文摘 | Zhiyuan Yu Guanghui Han Shutao Sun Xinning Jiang Rui Chen Fangjun Wang Ren’an Wu Mingliang Ye Hanfa Zou | 2009 | Analytica Chimica Acta2009，，1： | 1 |
| 6 | Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes显示文摘The human serum proteome is closely associated with the state of the body.Endogenous peptides derived from proteolytic enzymes cleaving on serum proteins are widely studied due to their potential application in disease-specific marker discovery.However,the reproducibility of peptidome analysis of endogenous peptides is significantly influenced by the proteolytic enzymes within body fluids,thereby limiting the clinical use of the endogenous peptides.We comprehensively investigated the N and C terminus of endogenous peptides using peptidomics.The cleavage site patterns of the N and C terminus and adjacent sites from all the identified endogenous peptides were highly conserved under different sample preparation conditions,including long-term incubation at 37℃ and pretreatment with repeated freeze-thaw cycles.Furthermore,a distinguishable cleavage site pattern was obtained when a different disease serum was analyzed.The conserved cleavage site pattern derived from proteolytic enzymes holds potential in highly specific disease diagnosis. | Fangjun Wang Jun Zhu Lianghai Hu Hongqiang Qin Mingliang Ye Hanfa Zou | 2012 | Protein & Cell2012，3，9： | 0 |
| 7 | Characterization and manipulation of the photosystem Ⅱ-semiconductor interfacial molecular interactions in solar-to-chemical energy conversion显示文摘Semi-artificial photosynthesis interfacing catalytic protein machinery with synthetic photocatalysts exhibits great potential in solar-to-chemical energy conversion. However, characterizing and manipulating the molecular integration structure at the biotic-abiotic interface remain a challenging task. Herein,the biointerface molecular integration details of photosystem II(PSII)-semiconductor hybrids, including the PSII orientation, interfacial microdomains, and overall structure modulation, are systematically interrogated by lysine reactivity profiling mass spectrometry. We demonstrate the semiconductor surface biocompatibility is essential to the PSII self-assembly with uniform orientation and electroactive structure.Highly directional localization of PSII onto more hydrophilic Ru/Sr Ti O_(3):Rh surface exhibits less disturbance on PSII structure and electron transfer chain, beneficial to the high water splitting activity.Further, rational modification of hydrophobic Ru_(2)S_(3)/Cd S surface with biocompatible protamine can improve the hybrid O_(2)-evolving activity 83.3%. Our results provide the mechanistic understanding to the structure–activity relationship of PSII-semiconductor hybrids and contribute to their rational design in the future. | Min He Wangyin Wang Zheyi Liu Wenxiang Zhang Jinan Li Wenming Tian Ye Zhou Yan Jin Fangjun Wang Can Li | 2022 | Journal of Energy Chemistry2022，31，7： | 0 |
| 8 | Pollution and Control Measures in Plant Tissue Culture in Orchid Plant显示文摘Contamination is a phenomenon that often occurs in the operation of plant tissue culture,and it is also one of the three major problems in plant tissue culture.Compared with browning and vitrification,contamination is more likely to occur,which brings great harm to scientific research and production practice.Its appearance will greatly affect the normal growth and differentiation of tissue culture materials,and will reduce the yield of cultivated plants to a certain extent.Therefore,we cannot underestimate any step in the tissue culture operation.This study summarized the reasons for its occurrence and how to formulate prevention and control measures based on recent research combined with the actual situation. | Fangjun LIAO Xiaoling YE Sumei HUANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
| 9 | Tissue Culture and Rapid Propagation System of Roselle(Hibiscus sabdariffa L.)显示文摘[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle plants as materials to study the suitable explants,the best disinfection method,the best growth medium,the best rooting medium and the transplanting and domestication method of roselle.[Results]The lateral buds of roselle were the best explants.Sterilizing with 0.1%mercuric chloride for 7 min showed a contamination rate of 5%and achieved a survival rate of 90%.With MS as the basic medium,adding 1.0 mg/L 6-BA and 0.5 mg/L IBA could obtain the best effect of bud induction.The medium with the highest proliferation rate was MS+6-BA 0.5 mg/L+BA 0.1 mg/L.On the basis of 1/2MS,adding 0.5 mg/L NAA+0.5 mg/L IBA could make adventitious buds root fastest and most,and greatly improve the propagation coefficient.And 1∶1 perlite rock:peat soil was the best transplanting substrate,with which the transplanting survival rate reached 95%.[Conclusions]This study provides technical reference for rapid cultivation and large-scale planting of roselle. | Zifan GUI Fangjun LIAO Xiaoling YE Sumei HUANG Liqing WANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
| 10 | Tissue Culture Application of Maranta arundinacea显示文摘The reproductive methods of ornamental arrowroot(Maranta arundinacea)mainly include plant division,cutting propagation,truncation and sprout promotion propagation,and tissue culture.Large-scale seedling production mainly relies on tissue culture.In the process of tissue culture,the culture conditions can be artificially controlled,and the plant materials used for tissue culture grow completely in artificially-provided culture media and microclimate environment,free from the adverse effects of catastrophic climate,seasonal and diurnal changes.Because the conditions are relatively uniform,tissue culture is very beneficial for plant growth and more convenient for stable long-term cultivation and production.In the process of tissue culture,the inoculation process is more important.How can we achieve standardized operation and reduce pollution during the inoculation process is worth exploring. | Fangjun LIAO Xiaoling YE Fanshu HUANG | 2023 | Agricultural Biotechnology2023，12，4： | 0 |
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