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| 1 | Detection of tumor stem cell markers in pancreatic carcinoma cell lines显示文摘BACKGROUND: Cancer of the pancreas is the fourth leading cause of cancer death in industrialized countries. In malignancy, actively proliferating cells may be effectively targeted and killed by anti-cancer therapies, but stem cells may survive and support re-growth of the tumor. Thus, new strategies for the treatment of cancer clearly will also have to target cancer stem cells. The goal of the present study was to determine whether pancreatic carcinoma cell growth may be driven by a subpopulation of cancer stem cells. Because previous data implicated ABCG2 and CD133 as stem cell markers in hematopoietic and neural stem/progenitor cells, we analyzed the expression of these two proteins in pancreatic carcinoma cell lines. METHODS: Five established pancreatic adenocarcinoma cell lines were analyzed. Total RNA was isolated and real- time RT-PCR was performed to determine the expression of ABCG2 and CD133. Surface expression of ABCG2 and CD133 was analyzed by flow cytometric analysis. RESULTS: All pancreatic carcinoma cell lines tested expressed significantly higher levels of ABCG2 than non-malignant fibroblasts or two other malignant non- pancreatic cell lines, i.e., SaOS2 osteosarcoma and SKOV3 ovarian cancer. Elevated CD133 expression was found in two out of five pancreatic carcinoma cell lines tested. Using flow cytometric analysis we confirmed surface expression of ABCG2 in all five lines. Yet, CD133 surface expression was detectable in the two cell lines, A818-6 and PancTu1, which exhibited higher mRNA levels.CONCLUSIONS: Two stem cell markers, ABCG2 and CD133 are expressed in pancreatic carcinoma cell lines. ABCG2 and/or CD133 positive cells may represent subpopulation of putative cancer stem cells also in this malignancy. Because cancer stem cells are thought to be responsible for tumor initiation and its recurrence after an initial response to chemotherapy, they may be a very promising target for new drug developments. | Monika Olempska Patricia Alice Eisenach Ole Ammerpohl Hendrik Ungefroren Fred Fandrich Holger Kalthoff | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，1： | 69 |
| 2 | Modern SEM-based mineral liberation analysis显示文摘 | Rolf Fandrich Ying Gu Debra Burrows Kurt Moeller | 2006 | International Journal of Mineral Processing2006，，1： | 2 |
| 3 | Induction of tolerance in clinical organ transplantation显示文摘 | Fandrich F | 2006 | Nephrol Dial Transplant2006，21，5： | 1 |
| 4 | Stand der Pfannen- metallurgie und Aktuelle Trends显示文摘 | Fandrich R Kleimt B Liebig H | 2011 | Stahl und Eisen2011，，131： | 1 |
| 5 | 胃穿孔术后并发坏死性筋膜炎一例显示文摘 | 吴胜东 蔡秀军 Fandrich | 2002 | 临床外科杂志2002，10，5： | 1 |
| 6 | Tumor necrosis factor-alpha hyporesponsiveness of rat intestinal mononuclear cells and whole portal venous blood after hemorrhagic 显示文摘 | Schroder J Kahlke V Fandrich F | 1998 | Critical Care Medicine1998，26，3： | 1 |
| 7 | Tolerance in clinical transplantation:progress,challenge or just a dream?显示文摘 | Fandrich F | 2011 | Langenbecks Arch Surg2011，396，4： | 1 |
| 8 | Preimplantation-stage stem cells induce long-term allogeneic graft acceptance without supplementary host conditioning显示文摘 | Fandrich F Lin X Chai G X | 2002 | Nat Med2002，8，2： | 1 |
| 9 | Detection of tumor stem cell markers in pancreatic carcinoma cell lines显示文摘 | Olempska M Eisenach P A Ammerpohl O Ungefroren H Fandrich F Kalthoff H | 2007 | Hepatobiliary Pancreat Dis Int2007，6，： | 1 |
| 10 | Tumor necrosis factoralph a hyporesponsiveness of rat intestinal mononuclear cells and whole portal venous blood after hemorrhagic显示文摘 | SCHRODER J KAHLKE V FANDRICH F | 1998 | Crit Care Med1998，26，3： | 1 |
| 11 | Modem SEM-based mineral liberation analysis显示文摘 | FANDRICH R GU Y BURROWS D MOELLER K | 2007 | International Journal of Mineral Processing2007，84，14： | 1 |
| 12 | Factors affecting germination of jointed goatgrass(Aelilops cylindrica ) seed显示文摘 | Fandrich L Mallory-Smith C A | 2006 | Weed Science2006，54，： | 1 |
| 13 | Preimplantation stage stem cells induce long term allogeneic graft acceptance without supplementary host conditioning显示文摘 | Fandrich F Lin X Chai GX | 2002 | Nat Med2002，8，2： | 1 |
| 14 | Ischemia-induced dephosphorylation of cardiomyocyte connexin-43 is reduced by okadaic acid and calyculin A but not fostriecin 显示文摘 | Jeyaraman M Tanguy S Fandrich RR | 2003 | Mol Cell Biochem2003，242，12： | 1 |
| 15 | Absorption and Fate of BAY MKH 6561 in Jointed Goatgrass and Downy Brome显示文摘 | FANDRICH L MCDONALD S K NISSEN S J | 2001 | Weed Science2001，49，2： | 1 |
| 16 | Glutamine dipeptides-supplemented parenteral nutrition reverses gut mucosal structure and intedenkin-6 rdease of rat intesanal mononclear cells afterh emorrhagic shock显示文摘 | Schroder J Kahlke V Fandrich F | 1998 | Shock1998，10，1： | 1 |
| 17 | Increased basic fibroblast growth factor accumulation and distinct patterns of localization in isoproterenol-induced cardiomyocyte injury 显示文摘 | Padua RR Fandrich RR Kardarmi E | 1993 | Growth Factors1993，8，4： | 1 |
| 18 | Exprerimental tracheal replacementusing a revascularized ileal segment for transplantation in rats 显示文摘 | Fandrich F Schroder DW | 1995 | J Surg Res1995，59，： | 1 |
| 19 | Ischemia induced)dephosphorylation of cardiomyocyte connexin-43 is reduced by okadaic acid and calyculin A but not fostriecin显示文摘 | Jeyaraman M Tanguy S Fandrich RR | 2003 | Mol Cell Biochem2003，242，： | 1 |
| 20 | Analysis of the virus popula- tion present in equine faeces indicates the presence of hundreds of uncharacterized virus genomes显示文摘 | Cann A J Fandrich SE Heaphy S | 2005 | Virus Genes2005，30，2： | 1 |
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