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| 1 | Connexin 43 hemichannels protect bone loss during estrogen deficiency显示文摘Estrogen deficiency in postmenopausal women is a major cause of bone loss,resulting in osteopenia,osteoporosis,and a high risk for bone fracture.Connexin 43 (Cx43) hemichannels (HCs) in osteocytes play an important role in osteocyte viability,bone formation,and remodeling.We showed here that estrogen deficiency reduced Cx43 expression and HC function.To determine if functional HCs protect osteocytes and bone loss during estrogen deficiency,we adopted an ovariectomy model in wild-type (WT) and two transgenic Cx43 mice:R76W (dominant-negative mutant inhibiting only gap junction channels) and Cx43 Δ130–136 (dominant-negative mutant compromising both gap junction channels and HCs).The bone mineral density (BMD),bone structure,and histomorphometric changes of cortical and trabecular bones after ovariectomy were investigated.Our results showed that the Δ130–136 transgenic cohort had greatly decreased vertebral trabecular bone mass compared to WT and R76W mice,associated with a significant increase in the number of apoptotic osteocyte and empty lacunae.Moreover,osteoclast surfaces in trabecular and cortical bones were increased after ovariectomy in the R76W and WT mice,respectively,but not in Δ130–136 mice.These data demonstrate that impairment of Cx43 HCs in osteocytes accelerates vertebral trabecular bone loss and increase in osteocyte apoptosis,and further suggest that Cx43 HCs in osteocytes protect trabecular bone against catabolic effects due to estrogen deficiency. | Liang Ma Rui Hua Yi Tian Hongyun Cheng Roberto Jose Fajardo Joseph J. Pearson Teja Guda Daniel Brian Shropshire Sumin Gu Jean X. Jiang | 2019 | Bone Research2019，7，2： | 11 |
| 2 | 北偏西大风对北京冬季生物气溶胶的影响显示文摘生物气溶胶对大气成云过程、生态系统演化和人体健康都有着重要的影响,目前对生物气溶胶浓度、组成和活性的变化规律认识不足.因此,在2013年1月和2015年1月在北京市清华大学校园内进行了采样和观测,以分析气象条件对生物气溶胶浓度和组成的影响.生物气溶胶浓度用生物气溶胶在线检测器WIBS-4A(waveband integrated bioaerosol sensor)测定,生物气溶胶中细菌群落的组成用16S rDNA测序的方法来测定.结果表明,北京冬季生物气溶胶数浓度范围在2~150 L^(-1).风是影响生物气溶胶的浓度和组成的重要因素.主导风向为北偏西30°,风速大于4 m·s-1的大风天气时,生物气溶胶的数浓度升高1个数量级,生物气溶胶中细菌群落的组成也发生急剧的变化.大风天气过后,生物气溶胶中细菌群落的组成缓慢恢复到大风前的状态. | 闫威卓 王步英 Oscar Fajardo Montana 蒋靖坤 郝吉明 | 2017 | 环境科学2017，38，9： | 5 |
| 3 | Six-day measurement of size-resolved indoor fluorescent bioaerosols of outdoor origin in an office显示文摘 | Yangyang Xie Oscar A. Fajardo Weizhuo Yan Bin Zhao Jingkun Jiang | 2017 | Particuology2017，15，2： | 4 |
| 4 | CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses显示文摘AIM: To investigate the influence of the CagA diversity in Helicobacter pylori (H. pylori ) strains from Colombia on the host cell biology. METHODS: Eighty-four H. pylori-cagA positive strains with different Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs patterns, isolated from patients with gastritis (n=17), atrophic gastritis (n=17), duodenal ulcer (n=16), intestinal metaplasia (n=16) and gastric cancer (n=18), were included. To determine the integrity of the cag pathogenicity island (cag PAI) we evaluated the presence of cagA, cagT, cagE, and cag10 genes by polymerase chain reaction. AGS gastric epithelial cellswere infected with each strain and assayed for translo-cation and tyrosine phosphorylation of CagA by western blot, secretion of interleukin-8 (IL-8) by enzyme-linked immuno sorbent assay after taking supernatants from cocultures and cell elongation induction. For cell elongation quantification, coculture photographs were taken and the proportion of 'hummingbird' cells (>15 μm) was determined. RESULTS: Overall 72% (60/84) of the strains were found to harbor a functional cag PAI. Levels of phos-phorylated CagA were significantly higher for isolates from duodenal ulcer than the ones in strains from gas-tritis, atrophic gastritis, intestinal metaplasia and gastric cancer (49.1% ± 23.1% vs 21.1% ± 19.5%, P < 0.02; 49.1% ± 23.1% vs 26.2%±14.8%, P<0.045; 49.1% ± 23.1% vs 21.5% ± 19.5%, P<0.043 and 49.1% ± 23.1% vs 29.5% ± 27.1%, P < 0.047 respectively). We observed variable IL-8 expression levels ranging from 0 to 810 pg/mL and from 8.8 to 1442 pg/mL at 6 h and 30 h post-infection, respectively. cagPAI-defective strains did not induce detectable levels of IL-8 at 6 h post-infection. At 30 h post-infection all strains induced IL-8 expression in AGS cells, although cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI (57.1 ± 56.6 pg/mL vs 513.6 ± 338.6 pg/mL,P < 0.0001). We did not observe differences in the extent of cell elongation induction between strains with a functional or a defective cagPAI in 6 h cocultures. At 24 h post infection strains with functionalcagPAI showed high diversity in the extent of hummingbird phenotype induction ranging from 7% to 34%. cag PAI defective strains induced significantly lower levels of elongation than strains with functional cag-PAI with one or more than one EPIYA-C motif (15.1% ± 5.2%vs 18.9% ± 4.7%,P < 0.03; and 15.1% ± 5.2% vs 20.0% ± 5.1%, P < 0.003 respectively). No differences were observed in cellular elongation inductionor IL-8 expression among H. pylori strains bearing one and more than one EPIYA-C motifs, neither at 6 h nor at 24 h of coculture. There were no associations between the levels of induction of cell elongation or IL-8 expression and number of EPIYA motifs or pathology. CONCLUSION: The present work describes a lack of association between H. pylori CagA protein EPIYA motifs variations from Colombian isolates and disease-associated cellular responses. | Carlos Alberto Fajardo Andrés Javier Quiroga Andrea Coronado Karen Labrador Nicole Acosta Pilar Delgado Carlos Jaramillo María Mercedes Bravo | 2013 | World Journal of Gastrointestinal Oncology2013，5，3： | 3 |
| 5 | Extended Modules and Ore Extensions显示文摘In this paper,we study extended modules for a special class of Oreextensions.We will assume that R is a ring and A will denote the Ore extensionA:=R[X1,...,xn;σ]for whichσis an automorphism of R,xixj=xjxi and xir=σ(r)xi,for every 1 | Viacheslav Artamonov Oswaldo Lezama William Fajardo | 2016 | Communications in Mathematics and Statistics2016，4，2： | 3 |
| 6 | Mitochondrial markers for the detection of four duck species and the specific identification of Muscovy duck in meat mixtures using the polymerase chain reaction显示文摘 | Irene Martín Teresa García Violeta Fajardo Inés López-Calleja María Rojas Pablo E. Hernández Isabel González Rosario Martín | 2007 | Meat Science2007，，4： | 2 |
| 7 | Experimental evaluation and simulation of volumetric shrinkage and warpage on polymeric composite reinforced with short natural fibers显示文摘聚合天赋增强纤维合成被挤出和注射塑造过程开发。与 Guadua angustifolia Kunth 的短自然纤维增强的高密度的聚乙烯的收缩和 warpage 被试验性的大小和计算机模拟分析。Autodesk Moldflow?并且固体工作?被采用在不同配置模仿注射部分的容量的收缩和 warpage:在收缩和聚合物的 warpage 行为上增强的 0 wt.% , 20 wt.% , 30 wt.% 和 40 wt.% 合成。变得明显在注射部分的容量的收缩和 warpage 上增强的限制效果。结果显示天赋的那容量的收缩合成与纤维增加被减少直到 58% ,而 warpage 显示出减小,与主要纤维内容形成 79% ~ 86% 。这些结果建议改进聚合自然增强纤维的 composites 的集会性质是自然纤维的高度有益的使用。 | Jonnathan D. SANTOS Jorge I. FAJARDO Alvaro R. CUJI Jaime A. GARCIA Luis E. GARZON Luis M. LOPEZ | 2015 | Frontiers of Mechanical Engineering2015，10，3： | 2 |
| 8 | EUS-guided fiducial markers placement with a 22-gauge needle for image-guided radiation therapy in pancreatic cancer显示文摘 | Raquel Dávila Fajardo Selma J. Lekkerkerker Astrid van der Horst Eelco Lens Jacques J. Bergman Paul Fockens Arjan Bel Jeanin E. van Hooft | 2013 | Gastrointestinal Endoscopy2013，，： | 2 |
| 9 | Bone Tissue Engineering Using Human Mesenchymal Stem Cells: Effects of Scaffold Material and Medium Flow显示文摘 | Lorenz Meinel Vassilis Karageorgiou Robert Fajardo Brian Snyder Vivek Shinde-Patil Ludwig Zichner David Kaplan Robert Langer Gordana Vunjak-Novakovic | 2004 | Annals of Biomedical Engineering2004，，1： | 2 |
| 10 | 生理性线粒体破碎是心脏适应能量需求增加的正常现象显示文摘线粒体在心脏中起着双重作用:负责满足能量需求和调节细胞凋亡。通常认为,线粒体的分裂和碎裂是病理性应激(如缺血)的结果,是线粒体质量差的指标,并导致线粒体自噬和细胞死亡。然而,最近的研究表明,抑制裂变也导致线粒体功能减弱和心脏损害,说明裂变对维持心脏和线粒体生物能量平衡十分重要。 | 刘莉 叶鹏 Coronado M Fajardo G Nguyen K Zhao M Kooiker KB Jung G Hu DQ Reddy S Sandoval E Stotland A Gottlieb RA Bernstein D | 2018 | 中华高血压杂志2018，26，1： | 2 |
| 11 | Stereotactic and sonographic large-core biopsy of nonpaIpable breast lesions:results of the Radiologic Diagnostic Oncology group V study显示文摘 | Fajardo LL Pisano ED Caudry DJ | 2004 | Acad Radiol2004，11，3： | 1 |
| 12 | Sequence analysis of the capsid protein gene of an isolate of Apple stem grooving virus, and its survey in Southern Brazil显示文摘 | NICKEL O FAJARDO T V M JELKMANN W KUHN G B | 2001 | Fitopatologia Brasileira2001，26，3： | 1 |
| 13 | A randomized, prospective, parallel group study comparing the Harmonic Scalpel to electrocautery in thyroidectomy显示文摘 | Cord6n C Fajardo R Ramirez J | 2005 | Surgery2005，137，3： | 1 |
| 14 | 2-Mercaptothiazoline modified mesoporous silica for mercury removal from aqueous media显示文摘 | Perez-Quintanilla D del Hierro D Fajardo M | 2006 | Journal of Hazardous Materials2006，134，123： | 1 |
| 15 | Cardiac pressure over?load hypertrophy is differentially regulated by -adrenergic recep?tor subtypes显示文摘 | Zhao MM Fajardo G Urashima T | 2011 | AmJ Physiol Heart Circ Physiol2011，301，4： | 1 |
| 16 | Volatile Composition and Key Flavour Compounds of Spirits from Unifloral Honeys 显示文摘 | Pino JA Fajardo M | 2011 | International Journal of Food Science : Technology2011，46，5： | 1 |
| 17 | Intraspecific trait variation and covariation in a widespread tree species Nothofagus pumilio in southern Chile显示文摘 | Fajardo A Piper F I | 2011 | New Phytologist2011，189，1： | 1 |
| 18 | Real-time TaqMan PCR for quantitative detection of cows' milk in ewes' milk mixtures 显示文摘 | IApez C Gonzalez I Fajardo V | 2007 | International Dairy Journal2007，17，7： | 1 |
| 19 | Factors associated with estrogen receptors-alpha(ER-alpha)and-beta(ERbeta)and progesterone receptor abundance in obese and non obege pre-and post-rnenopausal women显示文摘 | Fajardo ME Perez-Luque EL | 2006 | Steroids2006，71，6： | 1 |
| 20 | Preparation of 2-mercapto-benzo- thiazole-derivatized mesoporous silica and removal of Hg( II ) from aqueous solution显示文摘 | Pe rez-Quintanilla D Del Hierro I Fajardo M | 2006 | The Royal Society of Chemistry2006，8，： | 1 |
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