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| 1 | Correlation between rpoB gene mutation in Mycobacterium avium subspecies paratuberculosis and clinical rifabutin and rifampicin resistance for treatment of Crohn’s disease显示文摘AIM: To investigate overlapping regions of the rpoB gene previously involved with rifamycin resistance in M. tuberculosis and seek correlation between rpoB mutations in clinical MAP strains with susceptibility to RIF and RFB. METHODS: We designed a molecular-based PCR method for the evaluation of rifabutin (RFB) and rifampicin (RIF) resistance based on probable determinant regions within the rpoB gene of MAP, including the 81 bp variable site located between nucleotides 1363 and 1443. The minimum inhibitory concentration (MIC) for RIF was also determined against 11 MAP isolates in attempt to seek correlation with rpoB sequences. RESULTS: We determined that MAP strain 18 had an MIC of > 30 mg/L and ≤ 5 mg/L for RIF and RFB respectively, and a significant and novel rpoB mutation C1367T, compared to an MIC of ≤ 1.0 mg/L for both drugs in the wild type MAP. The 30-fold increase in the MIC was a direct result of the rpoB mutation C1367T, which caused an amino acid change Thr456 to Ile456 in the drug’s binding site. In addition, MAP strain 185 contained five silent rpoB mutations and exhibited an MIC comparable to the wild-type. Moreover, our in vitroselected mutation in MAP strain UCF5 resulted in the generation of a new resistant strain (UCF5-RIF16r) that possessed T1442C rpoB mutation and an MIC > 30 mg/L and > 10 mg/L for RIF and RFB respectively. Sequencing of the entire rpoB gene in MAP strains UCF4, 18, and UCF5-RIF16r revealed an rpoB mutation A2284C further downstream of the 81 bp variable region in UCF4, accounting for observed slight increase in MIC. In addition, no other significant mutations were found in strains 18 and UCF-RIF16r. CONCLUSION: The data clearly illustrates that clinical and in vitro-selected MAP mutants with rpoB mutations result in resistance to RIF and RFB, and that a single amino acid change in the beta subunit may have a significant impact on RIF resistance. Unconventional drug susceptibility testing such as our molecular approach will be beneficial for evaluation of antibiotic effectiveness. This molecular approach may also serve as a model for other drugs used for treatment of MAP infections. | Daniel R Beckler Sammer Elwasila George Ghobrial John F Valentine Saleh A Naser | 2008 | World Journal of Gastroenterology2008，14，17： | 2 |
| 2 | Expression ofa reflex of snapping/biting response to amino acids prior to the first exogenous feeding in salmonid alevins显示文摘 | Valentin(c)i(c) T B Lamb C F Caprio J | 1999 | Physiol Behav1999，67，4： | 1 |
| 3 | Electrochemical Ferrate Generation for Waste Water Treatment using Cast Irons with High Silicon Contents显示文摘 | Lescuras-Darrou V Lapicque F Valentin G | 2002 | J of Appl Electrochem2002，32，1： | 1 |
| 4 | The specific diversity indices for analysing phytoplankton succession: application to the Cabo Frio (RJ, Brazil)upwelling ecosystem显示文摘 | VALENTIN J L MACEDO - SAIDAH F E TENENBAUM D R | 1991 | Neritica1991，6，： | 1 |
| 5 | The response of straight mobile bed hannels to inbank and overbank flows 显示文摘 | Knight D W Brown F A Valentine E M | 1999 | Proc Instn of Civil Engineers Water Maritime and Energy Division London1999，136，4： | 1 |
| 6 | ACC/AHA/ESC 2006 guidelines for the management of patients with atrial fibrillation-executive summary: A report of the American College of Cardiology/American Heart Asso- ciation Task Force on practice guidelines and the European Society of Cardiology Committee for Practice Guidelines (Writing Committee to Revise the 2001 Guidelines for the Management of Patients with Atrial Fibrillation) 显示文摘 | Valentin F Lars E Rydrn | 2006 | Eur Heart J2006，27，8： | 1 |
| 7 | Effects of nutrient enrichment and small predator density on sea- grass ecosystems: an experimental assessment显示文摘 | Heck K L Pennock J R Valentine J F | 2000 | Lim- nology and Oceanography2000，45，5： | 1 |
| 8 | Antiplasmodial and cytotoxic activity of galipinine and other tetrahydroquinolines from Galipea officinalis显示文摘 | Jacquemond-Collet I Benoit-Vical F Valentin A | 2002 | Planta Med2002，68，1： | 1 |
| 9 | Cloning and recombinant expression of a structurally novel human se-creted phospholipaseA2显示文摘 | Gelb MH Valentin E Ghomashchi F | 2012 | J Biol Chem2012，275，51： | 1 |
| 10 | 查看详情显示文摘 | A B Sorokin F Quignard R Valentin | | 0，，： | 1 |
| 11 | Towards multiscale func- tions: enriching finite element spaces with local but not bubble- like functions 显示文摘 | Franca L P Madureira A L F Valentin | 2005 | Comput Methods Appl Mech Engrg2005，194，2729： | 1 |
| 12 | How venture capital shapes emerging bio-clusters:a cross-country comparison显示文摘 | VALENTIN F JENSEN R L DAHLGREN H | | 0，，03： | 1 |
| 13 | Regression of atherosclerotic lesions by high density lipoprotein plasma fraction in the Cholesterol-fed Rabbit显示文摘 | Badimon JJ Badimon L Valentin F | 1990 | J Clin Invest1990，85，： | 1 |
| 14 | The specific diversity indices fox analyzing phytoplankton succession:application to the Cabo Frio (R J,Brazil) upwelling ecosystem显示文摘 | Valentin J L Macedo-sadah F E Tenenbaum D R | 1991 | Neritica1991，6，： | 1 |
| 15 | Antiplasmodial and cytotoxic activity of galipinine and other tetrahydroquinolines from Galipea officinalis 显示文摘 | Jacquemond-Collet I Benoit-Vical F Valentin A | 2002 | Planta Med2002，68，1： | 1 |
| 16 | Edge shape effect on vibrational modes in graphene nanoribbons:A numerical study显示文摘 | Mazzamuto F Saint-Martin J Valentin A | 2011 | J Appl Phys2011，109，06： | 1 |
| 17 | N2-fixation and transfer in a field grown mycorrhizal corn and soybean显示文摘 | Chantal H Valentin F Donald L S | 1991 | Plant and Soil1991，133，： | 1 |
| 18 | Picobiliphytes:A marine picoplanktonic algal group with unknown affinities to other eukaryotes显示文摘 | Not F Valentin K Romari K | | 0，，5809： | 1 |
| 19 | Hydrogenisotopicvariability in lipids from Santa Barbara Basin sediments 显示文摘 | Li Chao Sessions A L Kinnaman F S Valentine D L | 2009 | Geochim Cosmochim Acta2009，73，48： | 1 |
| 20 | Losartan prevents thromboxane A2/prostanoid (TP) receptor mediated increase in microvascular permeability in the rat显示文摘 | Valentin JP Jover B Maffre M Bertolino F Bessac AM John GW | 1997 | Am J Hypertens1997，10，91： | 1 |
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