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| 1 | Effect of a gib- berellin-biosynthesis inhibitor treatment on the physicochemical properties of sorghum starch 显示文摘 | Enpeng Li Jovin Hasjim Sushil Dhital | 2011 | Journal of Cereal Science2011，53，3： | 1 |
| 2 | Codon Optimization of SMAP-29 Gene and Its Expression in Pichia pastoris显示文摘[Objective] This study aimed to optimize the codon of antimicrobial peptide SMAP-29 gene and express SMAP-29 in Pichia pastoris. [Method] According to the published amino acid sequence of SMAP-29, a gene encoding SMAP-29 (sheep myeloid antibacterial peptides-29) mature peptide was synthesized and was biased codon usage of Pichia pastoris. The synthesized gene was inserted into Pichia pastoris expression vector pPIC3.5K, transformed into Pichia pastoris strain GS115 by electroporation, and screened with G418 for high-copy recombinants. The methanol utility plus phenotype (Mut+) was selected with MM, MD and PCR. The correctly constructed recombinant strain was induced with methanol. Expression of SMAP-29 was analyzed by lysing the induced yeast cells with Tricine-SDS-PAGE. [Result] After induced with methanol for 2 d, a 3.2 kD induced band was detected in the cell lysate which was consistent to the predicted molecular weight of SMAP-29; the expression products purified with gel filtration chromatography showed significant antibacterial effect against Staphyloccocus aureus and Monilia albican but no significant antibacterial effect against Escherichia coli. [Conclusion] This study laid the foundation for the application of SMAP-29 in biomedicine, agriculture and other fields. | Yaojun REN Enpeng HE Xinhua WANG Xinwen BO | 2012 | Agricultural Biotechnology2012，1，4： | 1 |
| 3 | Isolation and identification of a novel cDNA that encodes human yrdC protein显示文摘 | Jinzhong Chen Chaoneng Ji Shaohua Gu Enpeng Zhao Jianliang Dai Lu Huang Ji Qian Kang Ying Yi Xie Yumin Mao | 2003 | Journal of Human Genetics2003，，4： | 1 |
| 4 | Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration显示文摘Deer antlers constitute a unique mammalian model for the study of both organ formation in postnatal life and annual full regeneration.Previous studies revealed that these events are achieved through the proliferation and differentiation of antlerogenic periosteum(AP)cells and pedicle periosteum(PP)cells,respectively.As the cells resident in the AP and the PP possess stem cell attributes,both antler generation and regeneration are stem cell-based processes.However,the cell composition of each tissue type and molecular events underlying antler development remain poorly characterized.Here,we took the approach of single-cell RNA sequencing(scRNA-Seq)and identified eight cell types(mainly THY1^(+)cells,progenitor cells,and osteochondroblasts)and three core subclusters of the THY1^(+)cells(SC2,SC3,and SC4).Endothelial and mural cells each are heterogeneous at transcriptional level.It was the proliferation of progenitor,mural,and endothelial cells in the activated antler-lineage-specific tissues that drove the rapid formation of the antler.We detected the differences in the initial differentiation process between antler generation and regeneration using pseudotime trajectory analysis.These may be due to the difference in the degree of stemness of the AP-THY1+and PP-THY1^(+)cells.We further found that androgen-RXFP2 axis may be involved in triggering initial antler full regeneration.Fully deciphering the cell composition for these antler tissue types will open up new avenues for elucidating the mechanism underlying antler full renewal in specific and regenerative medicine in general. | Hengxing Ba Xin Wang Datao Wang Jing Ren Zhen Wang Hai-Xi Sun Pengfei Hu Guokun Zhang Shengnan Wang Chao Ma Yusu Wang Enpeng Wang Liang Chen Tianbin Liu Ying Gu Chunyi Li | 2022 | Cell Regeneration2022，11，1： | 0 |
| 5 | Biological factors controlling starch digestibility in human digestive system显示文摘Starch digestion rate and location in the gastrointestinal tract(GIT)are critical for human health.This review aims to present a comprehensive summary on our current understanding of physiological,biochemical,anatomical and geometrical factors of human digestive system that are related to in vivo starch digestibility.It is shown that all digestive compartments including mouth,stomach,small intestine,and large intestine play critical roles in regulating the overall starch digestion process.A proper investigation of starch digestion pattern should thus be based on the consideration of all these compartments.Main biological factors are summarized as oral mastication and salivation,gastric emptying and motility,small intestinal enzymes and motility,large intestinal resistant starch(RS)-microbiota interactions and gut-brain feedback control,as well as glucose adsorption and hormonal feedback control.However,connections among these biological factors in determining starch digestive behaviors remain elusive.This is due to the inherent complexity of human GIT anatomy,motility and biochemical conditions,as well as ethical,financial and technical issues in conducting clinical studies.Much technological and scientific efforts from both clinical studies and in vitro simulation models are required for a better understanding of in vivo starch digestion behaviors. | Cheng Li Yiming Hu Songnan Li Xueer Yi Shuaibo Shao Wenwen Yu Enpeng Li | 2023 | Food Science and Human Wellness2023，12，2： | 0 |
| 6 | Hydrogel-mediated drug delivery for treating stroke显示文摘Stroke is a common disease and is the major cause of death and disability.It occurs and generates devastating neurological deficits when cerebral blood vessel is blocked(ischemic stroke,IS)or ruptured(hemorrhagic stroke,HS).Hydrogel,being biodegradable and biocompatible,have shown attractive advantages in stroke therapy as a new biomaterial with desirable mechanical properties and tunability of structure,owing to special ability to load different cargoes for multiple treatment strategies,such as pharmacotherapy based on drug-delivery systems and cell therapy including mesenchymal stem cells(MSCs)and neural progenitor cells(NPCs)for improving functional outcomes.However,a comprehensive review of the functional hydrogel for treatment of stroke is still lacking.Therefore,in this work,the main pathological mechanisms of stroke including IS and HS are comprehensively described.The benefits of hydrogel for stroke treatment are also summarized regarding the natural advantages and the delivery advantages.Simultaneously,the application development of hydrogel for treatment of stroke is highlighted.Finally,the unique considerations and challenges in the design and application of hydrogel is discussed for treatment of stroke and clinical application in the future. | Wenyan Yu Enpeng Gong Bingbing Liu Lei Zhou Chengyuan Che Shu Hu Zhenzhong Zhang Junjie Liu Jinjin Shi | 2023 | Chinese Chemical Letters2023，34，9： | 0 |
| 7 | Carrier-free programmed spherical nucleic acid for effective ischemic stroke therapy via self-delivery antisense oligonucleotide显示文摘Antisense oligonucleotide(ASO)for anti-apoptosis is emerging as a highly promising therapeutic agents for ischemic stroke with complex pathological environment.However,its therapeutic efficacy is seriously limited by a number of challenges including inefficient internalization,low blood-brain barrier(BBB)penetration,poor stability,and potential toxicity of the carrier.Herein,a carrier-free programmed spherical nucleic acid nanostructure is developed for effective ischemic stroke therapy via integrating multifunctional modules into one DNA structure.By co-encoding caspase-3-ASO and transferrin receptor(TfR)aptamer into circle template,the spherical nucleic acid nanostructure(TD)was obtained via self-assembly.The experimental results demonstrated that the developed TD displayed efficient BBB penetration capability(6.4 times)and satisfactory caspase-3 silence effect(2.3 times)due to the dense DNA packaging in TD.Taken together,our study demonstrated that the carrier-free programmed spherical nucleic acid nanostructure could significantly improve the therapeutic efficacy of ischemic stroke and was a promising therapeutic tool for various brain damage-related diseases. | Wenyan Yu Cuiping Xuan Bingbing Liu Lei Zhou Na Yin Enpeng Gong Zhenzhong Zhang Yinchao Li Kaixiang Zhang Jinjin Shi | 2023 | Nano Research2023，16，1： | 0 |
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