维普中文期刊产品整合服务
172篇 您的检索式:作者名="Elsayed K"
    题名 作者 年代 出处 被引量
1视黄酸及Stra8基因联合作用对雄性鸡胚胎干细胞分化的影响显示文摘旨在研究视黄酸(All-trans retinoic acid,RA)及Stra8(Stimulated by retinoic acid gene 8)基因联合作用对雄性鸡胚胎干细胞(Chicken embryonic stem cells,cESCs)分化的影响。选用PCR方法鉴定雄性cESCs为研究对象,转染Stra 8基因,对Stra8基因阳性cESCs使用RA进行12d连续诱导,同时设立Stra8基因转染组、RA诱导组及空白组对照,各组均进行3次重复试验。细胞形态学、qRT-PCR检测RA及Sira8基因对cESCs分化的影响。结果表明。转染Stra8基因进行RA诱导,在诱导12d后,出现精原干细胞样的圆形小克隆,其他组cESCs出现类胚体或解体;qRT-PCR鉴定发现各组中干细胞标记基因表达量随着培养时间的延长逐渐下降,同时检测到生殖细胞相关基因表达,转染Stra8基因并使用RA诱导组中表达量均高于对照组(P<0.01),6d表达量达到最高,然后逐渐下降。Stra8基因和RA的联合作用能够促进cESCs向着生殖细胞方向的分化,不仅改变了原有细胞形态,同时出现了生殖相关基因的表达。通过该方法可以在体外建立一个。ESCs诱导分化模型,将为生殖细胞的发生、增殖、分化及调控机理的研究和人类不孕不育症的治疗奠定良好的基础。张振韬 王丹 施青青 ELSAYED A K 张亚妮 韦光辉 朱睿 左其生 李东 刘堃 李碧春 2014畜牧兽医学报2014,45,1:3
2Modeling, analysis and optimization of aircyclones using artificiaJ neural network, response surface methodology and CFD simulation approaches 显示文摘Elsayed K Lacor C 2011Powder Technology2011,212,:1
3Novel multiplex PCR assay for claracterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillinresistant staphylococcus aureus显示文摘Zhang K McClure JA Elsayed S 0,,:1
4The effect of cyclone vortex finder dimensions on the flow pattern and performance using LES 显示文摘Elsayed K Laeor C 2013Comput Fluids2013,71,:1
5Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types Ⅰ to Ⅴ in methicillin - resistant Staphylococcus aureus显示文摘Zhang K McClure JA Elsayed S 2005J Clin Microbiol2005,43,10:1
6Prognosticsignificance of CD20 expression and Epstein-Barr virus(EBV) association in classical Hodgkin lymphoma in Ja-pan :a clinicopathologic study 显示文摘Elsayed A A Asano N Ohshima K 2014Pathol Int2014,64,7:1
7Channel-aware earliest dead- line due fair scheduling for wireless multimedia networks 显示文摘ELSAYED K M F KHATI'AB A K F 2006Wire- less Personal Communications2006,38,1:1
8Photodynamie therapy in dermatology: A review 显示文摘Choudhary S Nouri K Elsaie ML 2009Lasers Med Sci2009,24,6:1
9Novel multiplex PCR assay for characterization and conminant subtyping of staphylococcal cas- sette chromosome mee types I to V in methieillin-resistant Staphy- lococcus aureus 显示文摘Zhang K Mcclure J Elsayed S 2005Clin Microbiol2005,43,10:1
10Two steroidal alkaloids from Veratrum viride显示文摘ELSAYED K A MCCHESNEY J D HALIM A F 1995Phytochemistry1995,38,6:1
11Photodynamic therapy in dermatology: a review显示文摘Choudhary S Nouri K Elsaie ML 2009Lasers Meal Sci2009,24,6:1
12Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec Types I to V in me thicillin resistant Staphylococcusaureus 显示文摘Zhang K McClure JA Elsayed S 2005J Clin Microbi- ol2005,43,10:1
13Novel multiplex PCR assay for characterization and concomitant subtyping of Staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus显示文摘Zhang K McClure JA Elsayed S 2005J Clin Microbiol2005,43,10:1
14Effects of Foliar Application with Compost Tea and Filtrate Biogas Slurry Liquid on Yield and Fruit Quality of Washington Navel Orange (Citrus sinenesis Osbeck) Trees显示文摘Alaa K h Omar Elsayed B Belal Abd E1-Naiem 0,,07:1
15Design and construction of Q-switched Nd:YAG laser system for LIBS measurements显示文摘ELSAYED K IMAM H HARFOOSH A 2012Optics and Laser Technology2012,1,44:1
16Isolation of purified allergens (cod) by isoelectric focusing 显示文摘Elsayed S Aas K 1971Int Arch Allergy Appl Immunol1971,40,:1
17Novel Multiplex PCR Assay for Characterization and Concomitant Subtyping of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus显示文摘Zhang K Y McClure J A Elsayed S 2005J Clin Microbiol2005,43,10:1
18Novel multiplex PCR assay for characterization and concomitant subtyping of staph- ylococcal cassette chromosome mec types I to V in Methieillin- resistant Staphylococcus aureus 显示文摘Zhang K McClure J A Elsayed S 2004J Clin Mierohiol2004,42,7:1
19Comparison of the effects of short-and long-pulse durations when using a 585-nmpulsed dye laser in the treatment of new surgical sears 显示文摘Nouri K Elsaie ML Vejjabhinanta V 2010Lasers Med Sci2010,25,1:1
20Nutritional as- sessment of patients on hemodialysis in a large dial- ysis center 显示文摘A1 Saran K Elsayed S Molhem A 2011J Kidney Dis Transplant2011,,:1
返回顶部 每页显示:
共9页 首页 上一页 第1页 下一页 末页 /9 跳转

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1 渝公网安备 50019002500403号 违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费