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| 1 | Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-κB activation显示文摘AIM: Different strains of bifidobacteria were analysed for their effects on HT-29 intestinal epithelial cells (IECs) in in vitro models both of the non-inflamed and inflamed intestinal epithelium. METHODS: A reporter gene system in HT-29 cells was used to measure levels of NF-KB activation after challenge with bifidobacteria or after bacterial pre-treatment following LPS challenge. IL-8 protein and pro-inflammatory gene expression was investigated using normal HT-29 cells. RESULTS: None of the bifidobacteria tested induced activation of nuclear factor KB (NF-KB) indicating that bifidobacteria themselves do not induce inflammatory events in IECs. However, six out of eight bifidobacteria tested inhibited lipopolysaccharide- (LPS-) induced NFKB activation in a dose- and strain-dependent manner. In contrast, NF-KB activation in response to challenge with tumor necrosis factor-α(TNF-α) was affected by none of the tested bifidobacteria, indicating that the inhibitory effect of bifidobacteria is specific for LPS-induced inflammation in IECs. As shown with two of the six inhibitionpositive bifidobacteria, LPS-induced inhibition of NFKB activation was accompanied by a dose-dependent decrease of interleukin 8 (IL-8) secretion and by lower mRNA levels for IL-8, TNF-a, cyclooxygenase 2 (Cox-2), and intercellular adhesion molecule 1 (ICAM-1). CONCLUSION: Some strains of bifidobacteria are effective in inhibiting LPS-induced inflammation and thus might be appropriate candidates for probiotic intervention in chronic intestinal inflammation. | Christian U Riedel Francis Foata David Philippe Oskar Adolfsson Bernhard J Eikmanns Stephanie Blum | 2006 | World Journal of Gastroenterology2006，12，23： | 14 |
| 2 | 谷氨酸棒杆菌基因缺失菌株的定点构建显示文摘通过目标基因内部缺失片段的获得以及DNA重组交换等技术的有效运用 ,在氨基酸工业重要生产菌谷氨酸棒杆菌中 ,成功地定点构建了两个目标研究基因的单基因缺失菌株和双基因缺失菌株 ,并通过了PCR和测序等方法的分析验证。 | 阮红 Bernhard Eikmanns | 2002 | 微生物学报2002，42，4： | 8 |
| 3 | 转座子挽救法对转座子突变菌株中插入位点的定位分析显示文摘为寻找谷氨酸棒杆菌转座子插入突变菌株中的转座子插入位点 ,采用了转座子挽救法对转座子及其插入位点附近的序列进行分离 ,并测定插入位点相邻DNA序列 ,获得了三个转座子插入位点DNA序列 ,其中一个是柠檬酸合成酶基因 ,另两个为目前未知基因 ,暂命名为orfA和orfB。该方法简便易行 。 | 阮红 Bernhard Eikmanns | 2002 | 微生物学报2002，42，3： | 4 |
| 4 | Cloning,sequence analysis,expression,and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme显示文摘 | Eikmanns B J Rittmann D Sahm H | | 0，，： | 1 |
| 5 | Response of the Central Metabolism in Corynebacterium glutamicum to the use of an NADH-Dependent Glutamate Dehydrogenase显示文摘 | Achim Marx Bernhard J. Eikmanns Hermann Sahm Albert A. de Graaf Lothar Eggeling | 1999 | Metabolic Engineering1999，，1： | 1 |
| 6 | The PEP-pyruvate-oxaloacetate node as the switch point for carbon flux distribution in bacteria显示文摘 | SAUER U EIKMANNS B J | 2005 | FEMS Microbiology Reviews2005，29，4： | 1 |
| 7 | Physicians Appeals on the Dangers of Mobile Communication-what is the Evidence? Assessment of Public Health Data显示文摘 | Nieden A Z Dietz C Eikmann T Kiefer J Herr C E W | 2009 | International Journal of Hygiene and Environmental Health2009，，212： | 1 |
| 8 | Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production显示文摘 | Krause FS Blombach B Eikmanns BJ | 2010 | Applied and Environmental Microbiology2010，76，24： | 1 |
| 9 | Am- plification of three threonine biosynthetic genes in Corynebacterium glutamicum and its influence on car- bon flux in different strains显示文摘 | Eikmanns B J Metzger M Reinscheid D | 1991 | Applied Microbiology and Biotechnology1991，34，5： | 1 |
| 10 | Engineering Corynebacterium glutamicum for theproduction of pyruvate显示文摘 | Wieschalka S Blombach B Eikmanns B J | 2012 | Applied Microbiology andBiotechnology2012，94，2： | 1 |
| 11 | Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production显示文摘 | KRAUSE F S BLOMBACH B EIKMANNS B J | 2010 | Applied and Environmental Microbiology2010，76，24： | 1 |
| 12 | Effect of Bifidobacterium lactis on the Incidence of Nosocomial Infections in Very-Low-Birth-Weight Infants: A Randomized Controlled Trial显示文摘 | Mihatsch W A Vossbeck S Eikmanns B Hoegel J Pohlandt F | 2010 | Neonatology2010，，2： | 1 |
| 13 | Link between phosphate starvation and glycogen metabolism in Corynebacterium glutamicum, revealed by metabolomics显示文摘 | Woo H M Noack S Seibold G M Willbold S Eikmanns B J Bott M | 2010 | Applied and environmental microbiology2010，76，20： | 1 |
| 14 | The PEP-pyruvate-oxaloacetate node as the switch point for carbon flux distribution in bacteria 显示文摘 | Sauer U Eikmanns BJ | 2005 | FEMS Microbiology Reviews2005，29，： | 1 |
| 15 | amrG1基因在谷氨酸棒杆菌乙酸活化中的作用显示文摘谷氨酸棒杆菌Corynebacteriumglutamicum可以利用乙酸为碳源和能源进行生长.乙酸代谢中涉及乙酸活化的两个酶为磷酸转乙酰酶PTA和乙酸激酶AK,它们是由pta-ack操纵子经诱导表达产生的.采用转座子挽救法,我们从调控突变株C.glutamicumG25中获得了amrG1和amrG2两个目标基因.经分析鉴定,amrG1基因(NCBIGenBank接受号为AF532964)可能参与乙酸代谢调控,编码作用于pta-ack操纵子的一个调控因子.该调控因子基因序列全长732bp,开放阅读框含有243个氨基酸,分子量约为27kD.通过基因定点缺失和过量表达技术,在谷氨酸棒杆菌野生型菌株中分别构建了amrG1基因缺失菌株和表达菌株,并研究了它们在含有葡萄糖和/或乙酸不同碳源的基本培养基上生长时产生的PTA和AK酶活性特征.酶活性测定结果发现其中的amrG1基因缺失菌株和表达菌株存在着与野生型菌株不同的一系列酶学特征,分析显示:以野生型菌株为对照,amrG1基因缺失菌株在含有葡萄糖碳源的培养基上生长时表现出较高的PTA和AK酶活性,并且在葡萄糖和乙酸两种碳源上生长时表现出与乙酸碳源上生长时几乎同样的PTA和AK酶活性;amrG1基因过量表达对葡萄糖碳源上生长产生的PTA和AK酶活性有一定程度的抑制,即表现出与基因缺失情况相反的调控效应.根据以上结果分析。 | 阮红 R.Gerstmeir S. Schinke B.J. Eikmanns | 2004 | 中国科学(C辑)2004，34，3： | 1 |
| 16 | Characterization of the isocitrate lyase gene from Corynebacterium glutamicum biochemical analysis of the enzyme 显示文摘 | Rescheid D J Eikmanns B J Sahm H | 1994 | Microbiology1994，176，： | 1 |
| 17 | Cloning, Sequence Analysis, Expression, and Inactivation of the Coryne- bacterium glutamicum icd Gene Encoding Isocitrate Dehydrogenase and Biochemical Characterization of the Enzyme显示文摘 | EIKMANNS B J RITTMANN D SAHM H | 1995 | J Bacteriol1995，177，3： | 1 |
| 18 | Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme显示文摘 | Rescheid D J Eikmanns B J Sahm H | 1994 | Microbiology1994，140，： | 1 |
| 19 | 谷氨酸棒状杆菌在乙酸盐和葡萄糖基质上的蓝白生长筛选(英文)显示文摘在谷氨酸棒状杆菌乙酸盐代谢调控关键酶—异柠檬酸裂解酶 ace A基因两段启动子区域后面组装了无启动子的β-半乳糖苷酶报道基因 ,并且带上四环素抗性标记基因构建了两个检测质粒 p ROH7和 p ROHO.通过该两个质粒转化了谷氨酸棒状杆菌野生型菌株 Corynebacterium glutamicum ACTT14 75 2 ,分别获得了含有两个检测质粒的两种谷氨酸棒状杆菌 ,由此建立了谷氨酸棒状杆菌在乙酸盐和葡萄糖基质上的两个蓝白生长筛选系统 .结果显示当两种菌株生长在含有葡萄糖的基本培养基上时呈现白色菌落 ,而生长在含有乙酸盐的基本培养基上时呈现蓝色菌落 ;其中菌落颜色还随菌株中检测质粒p ROH7和 p ROHO的不同而呈现淡蓝色和深蓝色的变化 .含有检测质粒 p ROH7和 p ROHO菌落的颜色变化反映了细胞在葡萄糖和乙酸盐不同和碳源上的调控状态 。 | 阮红 Robert Gerstmeir Bernhard Eikmanns | 2002 | 浙江大学学报(农业与生命科学版)2002，28，2： | 1 |
| 20 | Current knowledge on isobutanol production with Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum显示文摘 | Bastian Blombach Bernhard J. Eikmanns | 2011 | Bioengineered Bugs2011，，6： | 1 |
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