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| 1 | Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation显示文摘Mesenchymal stem cell(MSC)-based treatments have shown promise for improving tendon healing and repair.MSCs have the potential to differentiate into multiple lineages in response to select chemical and physical stimuli, including into tenocytes. Cell elongation and cytoskeletal tension have been shown to be instrumental to the process of MSC differentiation. Previous studies have shown that inhibition of stress fiber formation leads MSCs to default toward an adipogenic lineage, which suggests that stress fibers are required for MSCs to sense the environmental factors that can induce differentiation into tenocytes. As the Rho/ROCK signal transduction pathway plays a critical role in both stress fiber formation and in cell sensation, we examined whether the activation of this pathway was required when inducing MSC tendon differentiation using rope-like silk scaffolds. To accomplish this, we employed a loss-of-function approach by knocking out ROCK,actin and myosin(two other components of the pathway) using the specific inhibitors Y-27632, Latrunculin A and blebbistatin, respectively. We demonstrated that independently disrupting the cytoskeleton and the Rho/ROCK pathway abolished the expression of tendon differentiation markers and led to a loss of spindle morphology. Together, these studies suggest that the tension that is generated by MSC elongation is essential for MSC teno-differentiation and that the Rho/ROCK pathway is a critical mediator of tendon differentiation on rope-like silk scaffolds. | Edward Maharam Miguel Yaport Nathaniel L Villanueva Takintope Akinyibi Damien Laudier Zhiyong He Daniel J Leong Hui B Sun | 2015 | Bone Research2015，3，3： | 4 |
| 2 | Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli显示文摘Background: There is growing evidence to support the beneficial effects of supplementing direct-fed microbials(DFM) on performance, health status, and immune responses of weaned pigs. Therefore, the objective of this study was to investigate dietary supplementation of Bacillus subtilis(DSM 25841) on growth performance, diarrhea, gut permeability and immunity of weaned pigs experimentally infected with a pathogenic F-18 Escherichia coli(E. coli).Results: The F18 E. coli infection reduced(P < 0.05) growth performance and intestinal villi height, whereas increased(P < 0.05) diarrhea and transcellular and paracellular permeability in the jejunum compared with non-challenged control. Supplementation of Bacillus subtilis linearly enhanced average daily gain of E. coli infected pigs from d 0 to 5 post-inoculation(PI)(P < 0.05) and d 0 to 11 PI(P = 0.058). Supplementation of high dose of Bacillus subtilis reduced(P < 0.05) both transcellular and paracellular permeability on d 5 and d11 PI compared with the E. coli infected pigs fed with control diet. E. coli infection up-regulated(P < 0.05)the m RNA expression of SLC5 A10(soluble carrier family 5 member 10) and MUC2(mucin 2) on d 5 PI, but down-regulated(P < 0.05) expression of SLC5 A10, MUC2, and CLDN1 on d 11 PI in jejunal mucosa when pigs were fed with the control diet. Supplementation of Bacillus subtilis linearly up-regulated(P < 0.05) the m RNA expression of CFTR and ZO1 on d 5 PI and SLC5 A10 and MUC2 on d 11 PI in jejunal mucosa of E. coli infected pigs. In addition, E. coli infection increased(P < 0.05) the m RNA expression of several immune genes(IL1 A, IL1 B, and IL7 on d 5 PI, and IL1 B, IL6, IL7, and TNF on d 11 PI) in the ileal mucosa of weaned pigs. Inclusion of Bacillus subtilis to control diet linearly down-regulated gene expression of IL1 A on d 5 PI(P = 0.07) and IL6 on d 11 PI(P < 0.05) in ileal mucosa of E. coli infected pigs.Conclusions: Supplementation of Bacillus subtilis(DSM 25841) enhanced growth rate and improved gut barrier function of weaned pigs experimentally infected with a pathogenic E. coli. | Kwangwook Kim Yijie He Xia Xiong Amy Ehrlich Xunde Li Helen Raybould Edward R.Atwill Elizabeth A.Maga Jens JΦrgensen Yanhong Liu | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 3 |
| 3 | An Adaptive Grouping Scheme in Ultra-Dense IEEE 802.11ax Network Using Buffer State Report Based Two-Stage Mechanism显示文摘IEEE 802.11ax,which is an emerging WLAN standard,aims at providing highly efficient communication in ultra-dense wireless networks.However,due to a large number of stations(STAs)in the ultra-dense device deployment scenarios,the potentially high packet collision rate significantly decreases the communication efficiency of WLAN.In this paper,we propose an adaptive STA grouping scheme to overcome this dense network challenge in IEEE 802.11ax by using Buffer State Report(BSR)based Two-stage Mechanism(BTM).In order to achieve the optimal efficiency of BSR delivery,we analyze the functional relationship between STA number in group and Resource Unit(RU)efficiency.Based on this analysis results,an adaptive STA grouping algorithm with variable group size is proposed to achieve efficient grouping in BTM.The numerical results demonstrate that the proposed adaptive BTM grouping algorithm significantly improves the BSR delivery efficiency and the throughput of overall system and each STA in the ultra-dense wireless network. | Jiyang Bai He Fang Junghoon Suh Osama Aboul-Magd Edward Au Xianbin Wang | 2019 | China Communications2019，16，9： | 2 |
| 4 | 吡格列酮对高糖诱导血管内皮细胞凋亡的影响及作用机制显示文摘吡格列酮可抑制高糖诱导的血管内皮细胞凋亡,其机制可能与抑制高糖诱导的酸性鞘磷酯酶活性升高,减少细胞内神经酰胺产生相关。 | 黄丹丹 雷闽湘 Xingxuan He Edward H. Schuchman 陈本美 | 2009 | 中国糖尿病杂志2009，17，9： | 2 |
| 5 | Steroid hormones affect limbic afterdischarge thresholds and kindling rates in adult female rats显示文摘 | Edwards HE Burnham WM Mendonca A | 1999 | Brain Res1999，838，12： | 1 |
| 6 | Localization of neurosurgically implanted electrodes viaphotograph MRI radiograph coregistration显示文摘 | Dalal SS Edwards E Kirsch HE | 2008 | Journal of Neuroscience Methods2008，174，： | 1 |
| 7 | Prenatal stress alters seizure thresholds and the development of kindled seizures in infant and adult rats 显示文摘 | Edwards HE Dortok D Tam J Won D Burnham WM | 2002 | Horm Behavior2002，42，4： | 1 |
| 8 | Evaluating student learning:an Australian case study显示文摘 | Edwards HE Chapman H Nash RE | 2001 | Nursing & Health Sciences2001，3，4： | 1 |
| 9 | Infectious bursal disease virus structural proteins expressed in a baculovirus recombinant confer protection in chickens 显示文摘 | Vakharia V N Snyder D B He J Edwards G H Savage P K Mengel-Whereat S A | 1993 | J Gen Virol1993，74，6： | 1 |
| 10 | Testosterone and its metabolites affect afterdischarge thresholds and the development of amygdala kindled seizures显示文摘 | Edwards HE Burnham WM and MacLusky NJ | 1999 | BrainRes1999，14，： | 1 |
| 11 | Partial and generalized seizures affect reproductive physiology differentially in the male rat显示文摘 | Edwards HE Burnham WM MacLusky NJ | 1999 | Epilepsia1999，40，： | 1 |
| 12 | The Holocene Asian monsoon: Links to solar changes and north Atlantic climate显示文摘 | Wang Y J Cheng H Edwards R L He Y Kong X An Z S Wu J Y Kelly M G Dykoski C A Li X D | 2005 | Science2005，308，5723： | 1 |
| 13 | Household CO and PM measured as part of a review of China's National Improved Stove Program显示文摘 | Edwards R D Liu Y He G | | 0，，03： | 1 |
| 14 | lmproving pain mana- gement by nurses:a pilot peer intervention program 显示文摘 | Edwards HE Nash RE Yates PM | 2001 | Nuts Health Sci2001，3，3： | 1 |
| 15 | Oxidation of benzyl alcohol using supported gold-palladium nanop- articles 显示文摘 | Miedziak P J He Q Edwards J K | 2011 | Catal Today2011，163，： | 1 |
| 16 | Etiology of temporomandibular joint ankylosis secondary to condylar fractures: the role of concomitant mandibular fractures 显示文摘 | He D Edward E 3rd Zhang Y | 2008 | J Oral Maxillofac Surg2008，66，1： | 1 |
| 17 | TRPV1 Channels Mediate Longterm Depression at Synapses on Hippocampal Interneurons显示文摘 | Gibson HE Edwards JG Page RS | 2008 | Neuron2008，57，5： | 1 |
| 18 | The Holocene Asian monsoon Sinks to solar changes and North Atlantic climate显示文摘 | WANG Y CHENG H EDWARDS RL HE Y KONG X AN Z WU J KELLY M J DYKOSKI C A LI X | 2005 | Science2005，8，： | 1 |
| 19 | Genome-Wide search for loss of heterozygosity in Chinese patients with sporadic colorectal cancer显示文摘 | Zhihai Peng Fang Zhang Chongzhi Zhou Yun Ling Shaochun Bai Wanqing Liu Guoqiang Qiu Lin He Liwei Wang Daoyan Wei Edward Lin Keping Xie | 2003 | International Journal of Gastrointestinal Cancer2003，，1： | 1 |
| 20 | Barriers to effective cancer pain management:a survey of hospitalized cancer patients in Australia显示文摘 | Yates PM Edwards HE Nash RE | 2002 | J Pain Symptom Manage2002，23，5： | 1 |
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