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387篇 您的检索式：作者名="Eastham"

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	题名	作者	年代	出处	被引量
1	人胚胎干细胞培养及其cDNA文库的构建显示文摘目的构建人胚胎干细胞cDNA文库,为筛查疾病抗原基因奠定基础。方法培养和收集人胚胎干细胞克隆,提取胚胎干细胞总RNA,分离纯化mRNA,利用mRNA作模板反转录合成双链cDNA,双链DNA经pfu-DNA聚合酶将链末端补平,与EcoRⅠ接头连接,XhoⅠ酶切消化产生粘端。用Sepharose CL2B柱分离纯化及除去小分子cDNA片段,与Uni-ZAP XR噬菌体连接,体外转化XL1-blue MRF'菌,建成cDNA文库并计算重组效率。对cDNA文库进行扩增,测定扩增文库的滴度。阳性重组子用EcoRⅠ+XhoⅠ双酶切鉴定插入片段的大小。结果构建成含1.2×106重组子的人胚胎干细胞cDNA文库,重组子插入外源DNA片段长度不小于1000kb。结论已成功构建人胚胎干细胞cDNA文库,适合用于筛选目的基因克隆。	周素芳 Angela M Eastham Peter L Stern	2007	中国现代医学杂志2007，17，17：	5
2	Positive Surgical Margins After Radical Prostatectomy: A Systematic Review and Contemporary Update显示文摘	Ofer Yossepowitch Alberto Briganti James A. Eastham Jonathan Epstein Markus Graefen Rodolfo Montironi Karim Touijer	2014	European Urology2014，，：	2
3	Postoperative nomogram predicting the 10-year probability of prostate cancer recurrence after radical prostatectomy显示文摘	Stephenson AJ Scardino PT Eastham JA	2005	J Clin Oncol2005，23，28：	1
4	Pelvie lymph node dissection in prostate cancer 显示文摘	Briganti A Blute ML Eastham JH	2009	Eur Urol2009，55，6：	1
5	A preoperative nomogram for disease recurrence following radical prostatectomy for prostate cancer显示文摘	Kattan M W Eastham J A Stapleton A M	1998	J Natl Cancer Inst1998，90，10：	1
6	Prostate biopsy techniques and indications:When,where,and how显示文摘	Scherr DS Eastham J Ohori M	2002	Semin Urol Oncol2002，20，1：	1
7	A preoperative nomogram for disease recurrence following radical prostatectomy for prostate cancer显示文摘	Kattan MW Eastham JA Stapleton AM	1998	J Natl Cancer Inst1998，90，10：	1
8	Further studied on an improved finite element method for moving conductor eddy current problems 显示文摘	Zhenning Liu Anthony R Eastham Graham E Dawson	1994	IEEE Transaction on Magnetics1994，30，5：	1
9	Angiographie embolization of renal stab wounds显示文摘	Eastham JA Wilson TG Larsen DW	1992	Urology1992，148，：	1
10	Role of ATP-sensitive potassium channels in coronary microvascular autoregulatory responses显示文摘	Lamping KG Eastham CL	1991	Circ Res1991，69，：	1
11	Angiographic embolization of renal stab wounds 显示文摘	EASTHAM J A WILSON T G LARSEN D	1992	J Urol1992，148，：	1
12	A formulation for low frequency eddy current solutions显示文摘	Rodger D Eastham J F	1983	IEEE Transactions on Magnetics1983，19，11：	1
13	Design of linear induction drives by field analysis and finite element techniques显示文摘	G E Dawson A R Eastham J F Gieras		0，，05：	1
14	Risk factors for urinary incontinence after radical prostatectomy显示文摘	Eastham J A Kattan M W Rogers E	1996	J Urol1996，156，5：	1
15	Effects of chronic hypertension and left ventricular hypertrophy on thr incidence of sedden cardiac death following coronary occlusion in consciousdogs显示文摘	Koyanagis Eastham CL Marcus ML	1982	Circulation1982，65，：	1
16	A formulation for low frequency eddy current solution显示文摘	Rodger D Eastham J F	1983	IEEE Trans Magn1983，19，11：	1
17	In vivo gene therapy with p^53 or p^21 adenovirus for prostate cancer显示文摘	Eastham JA Hall SJ Sehgal I	1995	Cancer Res1995，55，：	1
18	Growth of shoots and roots, and interception of radiation by wheat and lupin crops on a shallow, duplex soil in response to time of sowing显示文摘	Gregory P J Eastham J	1996	Australian Journal of Agricultural Research1996，47，42：	1
19	Positive surgical margins after radical prostatectomy: a systematic review and contemporary updale 显示文摘	Yossepowitch O Briganti A Eastham JA	2014	Eur Urol2014，65，2：	1
20	A new longitudinal end effect factor for linear induction motors显示文摘	Gieras J F Dawson G E Eastham A R	1987	IEEE Transactions on Magnetics1987，2，1：	1

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