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| 1 | Genome-wide identification and expression analysis of NIN-like Protein(NLP)genes reveals their potential roles in the response to nitrate signaling in watermelon显示文摘To balance the relationship between high yield and low nitrogen supply,the nitrogen utilization efficiency of watermelon needs to be improved urgently.Nodule inception-like Protein(NLP)transcription factors play a key node role in nitrate response and growth and development of plant,however,comprehensive analysis of the NLP gene family in watermelon is unclear.This study explored the functional classification,evolutionary characteristics,and expression profile of the ClNLP gene family.Three ClNLPs were categorized into three groups according to their gene structure and phylogeny.All of them contained the conserved RWP-RK and PB1 domains.Evolutionary analysis of ClNLPs revealed that ClNLP1 and ClNLP3 underwent strong purified selection.In addition,cis-acting elements related to plant hormones and abiotic stresses were present in the ClNLP promoter.According to tissue-specific analysis ClNLP was widely expressed in roots,stems,leaves,flowers and fruits,and ClNLP1 was significantly induced in the roots of different nitrogen utilization varieties under different nitrate nitrogen supply.The SRTING functional protein association network suggested that ClNLP1 is associated with most genes,such as NRT1.1,NRT2.1,NIA1,and NIR1,and the dual-luciferase reporter assay found that ClNLP1 positively regulates the expression of ClNRT2.1.We speculated that ClNLP1 might play a central role in regulating the response of watermelon to nitrate nitrogen. | Gaopeng Yuan Dexi Sun Yifan Wang Guolin An Weihua Li Wenjing Si Junpu Liu Yingchun Zhu | 2022 | Horticultural Plant Journal2022，8，5： | 2 |
| 2 | Oil-field wastewater treatment by hybrid membrane-aerated biofilm reactor (MABR) system显示文摘 | Peng Li Dexi Zhao Yunge Zhang Linquan Sun Huimin Zhang Meirong Lian Baoan Li | 2015 | Chemical Engineering Journal2015，，： | 1 |
| 3 | Composite topological nodal lines penetrating the Brillouin zone in orthorhombic AgF_(2)显示文摘It has recently been found that nonsymmorphic symmetries can bring many exotic band crossings.Here,based on symmetry analysis,we predict that materials with time-reversal symmetry in the space group of Pbca(No.61)possess rich symmetry-enforced band crossings,including nodal surfaces,fourfold degenerate nodal lines and hourglass Dirac loops,which appear in triplets as ensured by the cyclic permutation symmetry.We take Pbca AgF_(2) as an example in real systems and studied its band structures with ab initio calculations.Specifically,in the absence of spin-orbit coupling(SOC),besides the above-mentioned band degeneracies,this system features a nodal chain and a nodal armillary sphere penetrating the Brillouin zone(BZ).While with SOC,we find a new configuration of the hourglass Dirac loop/chain with the feature traversing the BZ,which originates from the splitting of a Dirac loop confined in the BZ.Furthermore,guided by the bulk-surface correspondence,we calculated the surface states to explore these bulk nodal phenomena.The evolution of these interesting nodal phenomena traversing the BZ under two specific uniaxial strains is also discussed. | Dexi Shao Huaiqiang Wang Tong Chen Pengchao Lu Qinyan Gu Li Sheng Dingyu Xing Jian Sun | 2019 | npj Computational Materials2019，，1： | 0 |
| 4 | Watermelon domestication was shaped by stepwise selection and regulation of the metabolome显示文摘Although crop domestication has greatly aided human civilization,the sequential domestication and regulation of most quality traits remain poorly understood.Here,we report the stepwise selection and regulation of major fruit quality traits that occurred during watermelon evolution.The levels of fruit cucurbitacins and flavonoids were negatively selected during speciation,whereas sugar and carotenoid contents were positively selected during domestication.Interestingly,fruit malic acid and citric acid showed the opposite selection trends during the improvement.We identified a novel gene cluster(CGC1,cucurbitacin gene cluster on chromosome 1)containing both regulatory and structural genes involved in cucurbitacin biosynthesis,which revealed a cascade of transcriptional regulation operating mechanisms.In the CGC1,an allele caused a single nucleotide change in Cl ERF1 binding sites(GCC-box)in the promoter of Cl Bh1,which resulted in reduced expression of Cl Bh1 and inhibition of cucurbitacin synthesis in cultivated watermelon.Functional analysis revealed that a rare insertion of 244 amino acids,which arose in C.amarus and became fixed in sweet watermelon,in Cl OSC(oxidosqualene cyclase)was critical for the negative selection of cucurbitacins during watermelon evolution.This research provides an important resource for metabolomics-assisted breeding in watermelon and for exploring metabolic pathway regulation mechanisms. | Pingli Yuan Congping Xu Nan He Xuqiang Lu Xingping Zhang Jianli Shang Hongju Zhu Chengsheng Gong Hanhui Kuang Tang Tang Yong Xu Shuangwu Ma Dexi Sun Weiqin Zhang Muhammad J.Umer Jian Shi Alisdair R.Fernie Wenge Liu Jie Luo | 2023 | Science China(Life Sciences)2023，66，3： | 0 |
| 5 | Large Spin Hall Conductivity and Excellent Hydrogen Evolution Reaction Activity in Unconventional PtTe1.75 Monolayer显示文摘Two-dimensional(2D)materials have gained lots of attention due to the potential applications.In this work,we propose that based on first-principles calculations,the(2×2)patterned PtTe_(2)monolayer with kagome lattice formed by the well-ordered Te vacancy(PtTe_(1.75))hosts large and tunable spin Hall conductivity(SHC)and excellent hydrogen evolution reaction(HER)activity.The unconventional nature relies on the A1@1b band representation of the highest valence band without spin–orbit coupling(SOC).The large SHC comes from the Rashba SOC in the noncentrosymmetric structure induced by the Te vacancy.Even though it has a metallic SOC band structure,theℤ_(2)invariant is well defined because of the existence of the direct bandgap and is computed to be nontrivial.The calculated SHC is as large as 1.25×10^(3)h/e(Ωcm)^(−1)at the Fermi level(EF).By tuning the chemical potential from EF−0.3 to EF+0.3 eV,it varies rapidly and monotonically from−1.2×10^(3)to 3.1×10^(3)h/e(Ωcm)^(−1)In addition,we also find that the Te vacancy in the patterned monolayer can induce excellent HER activity.Our results not only offer a new idea to search 2D materials with large SHC,i.e.,by introducing inversion–symmetry breaking vacancies in large SOC systems,but also provide a feasible system with tunable SHC(by applying gate voltage)and excellent HER activity. | Dexi Shao Junze Deng Haohao Sheng Ruihan Zhang Hongming Weng Zhong Fang Xing-Qiu Chen Yan Sun Zhijun Wang | 2023 | Research2023，，3： | 0 |
| 6 | Deadlock Detection in FPGA Design: A Practical Approach显示文摘Formal verification of VHSIC Hardware Description Language(VHDL) in Field-Programmable Gate Array(FPGA) design has been discussed for many years. In this paper we provide a practical approach to do so. We present a semi-automatic way to verify FPGA VHDL software deadlocks, especially those that reside in automata.A domain is defined to represent the VHDL modules that will be verified; these modules will be transformed into Verilog models and be verified by SMV tools. By analyzing the verification results of SMV, deadlocks can be found;after looking back to the VHDL code, the deadlocking code is located and the problem is solved. VHDL verification is particularly important in safety-critical software. As an example, our solution is applied to a Multifunction Vehicle Bus Controller(MVBC) system for a train. The safety properties were tested well in the development stage, but experienced a breakdown during the long-term software testing stage, which was mainly caused by deadlocks in the VHDL software. In this special case, we managed to locate the VHDL deadlocks and solve the problem by the FPGA deadlock detection approach provided in this paper, which demonstrates that our solution works well. | Dexi Wang Fei He Yangdong Deng Chao Su Ming Gu Jiaguang Sun | 2015 | Tsinghua Science and Technology2015，20，2： | 0 |
| 7 | Hepatitis B Virus Induces Microtubule Stabilization to Promote Productive Infection through Upregulating Microtubule-associated Protein 1S显示文摘Background and Aims:Continuous release and transmission of hepatitis B virus(HBV)is one of the main factors leading to chronic hepatitis B(CHB)infection.However,the mechanism of HBV-host interaction for optimal viral transport is unclear.Hence,we aimed to explore how HBV manipulates microtubule-associated protein 1S(MAP1S)and microtubule(MT)to facilitate its transport and release.Methods:The expression of MAP1S or acetylated MT was investigated by immunofluorescence,RT-PCR,immunoblotting,and plasmid transfection.MAP1S overexpression or knockdown was performed by lentiviral infection or shRNA transfection,respectively.HBV DNA was quantified using q-PCR.Results:Significantly higher level of MAP1S in HepG2215 cells compared with HepG2 cells was detected using RT-PCR(p<0.01)and immunoblotting(p<0.001).Notably,stronger MAP1S expression was observed in the liver tissues of patients with CHB than in healthy controls.MAP1S overexpression or knockdown demonstrated that MAP1S promoted MT acetylation and reduced the ratio of HBV DNA copies inside to outside cells.Further,transfection with the hepatitis B virus X protein(HBx)-expressing plasmids induced significantly higher level of MAP1S than that in controls(p<0.0001),whereas HBVX−mutant-encoding HBV proteins(surface antigen,core protein,and viral DNA polymerase)hardly affected its expression.Conclusions:These results demonstrate that HBx induces the forma tion of stable MTs to promote the release of HBV particles through upregulating MAP1S.Thus,our studies delineate a unique molecular pathway through which HBV manipulates the cytoskeleton to facilitate its own transportation,and indicate the possibility of targeting MAP1S pathway for treatment of patients with CHB. | Yuanyue Guan Bin Sun Shijie Zhang Yuan Zhuang Yanxiang Huang Minghua Lin Rongling Zheng Dexi Chen Ying Shi Yanjun Wang | 2022 | Journal of Clinical and Translational Hepatology2022，10，3： | 0 |
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