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| 1 | Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice显示文摘Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. | WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui ZHENG Xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong | 2019 | Rice science2019，26，2： | 8 |
| 2 | Multi-threshold second-order phase transition in laser显示文摘We present a theory of the multi-threshold second-order phase transition,and experimentally demonstrate the multi-threshold secondorder phase transition phenomenon.With carefully selected parameters,in an external cavity diode laser system,we observe secondorder phase transition with multiple(three or four) thresholds in the measured power-current-temperature three dimensional phase diagram.Such controlled death and revival of second-order phase transition sheds new insight into the nature of ubiquitous secondorder phase transition.Our theory and experiment show that the single threshold second-order phase transition is only a special case of the more general multi-threshold second-order phase transition,which is an even richer phenomenon. | ZHUANG Wei YU DeShui LIU ZhiWen CHEN JingBiao | 2011 | Chinese Science Bulletin2011，56，35： | 4 |
| 3 | HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley显示文摘Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled manner.In barley,Hv WRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus,Blumeria graminis f.sp.hordei(Bgh).However,the molecular features of Hv WRKY2 in its DNA-binding and repressor functions,as well as its target genes,are uncharacterized.We show that the W-box binding of Hv WRKY2 requires an intact WRKY domain and an upstream sequence of~75 amino acids,and the Hv WRKY2 W-box binding activity is linked to its repressor function in disease resistance.Chromatin immunoprecipitation(ChIP)-seq analysis identified HvCEBiP,a putative chitin receptor gene,as a target gene of Hv WRKY2 in overexpressing transgenic barley plants.ChIP-qPCR and Electrophoretic Mobility Shift Assay(EMSA)verified the direct binding of Hv WRKY2 to a W-boxcontaining sequence in the HvCEBiP promoter.Hv CEBiP positively regulates resistance against Bgh in barley.Our findings suggest that Hv WRKY2 represses barley basal immunity by directly targeting pathogen-associated molecular pattern(PAMP)recognition receptor genes,suggesting that Hv CEBiP and likely chitin signaling function in barley PAMP-triggered immune responses to Bgh infection. | Deshui Yu Renchun Fan Ling Zhang Pengya Xue Libing Liao Meizhen Hu Yanjun Cheng Jine Li Ting Qi Shaojuan Jing Qiuyun Wang Arvind Bhatt Qian-Hua Shen | 2023 | The Crop Journal2023，11，1： | 1 |
| 4 | An in situ study of inorganic nitrogen flow under different fertilization treatments on a wheat–maize rotation system surrounding Nansi Lake, China显示文摘 | Deshui Tan Lihua Jiang Shuying Tan Fuli Zheng Yu Xu Rongzong Cui Mei Wang Jing Shi Guosheng Li Zhaohui Liu | 2013 | Agricultural Water Management2013，，： | 1 |
| 5 | Optimization of Protective Agent Formulation for Soybean Rhizobium Strain HW-05 by Response Surface Methodology显示文摘[Objective] T his study aimed to investigate the protective effects of proteins,salts,sugars and trace elements on soybean rhizobium strain HW-05 by response surface methodology. [Method]Different types,combinations,ratios and concentrations of protective agents were designed. Under simulated conditions,the optimal protective agent formulation was screened for improving the survival rate and survival time of soybean rhizobium strain HW-05. The optimal combination of significant factors was determined by Box-Behnken central composite design. [Result] T hree significant factors affecting effective number of viable cells were screened,including peptone,xanthan gum and Na Cl. The final concentration of each compound was optimized: 0. 13% peptone,0. 011% xanthan gum,0. 30%Na Cl. [Conclusion] After addition of protective agent and preservation at room temperature for six months,effective number of viable cells of soybean rhizobium strain HW-05 reached 3.185 × 10~8 CFU / ml. The survival rate of HW-05 cells was improved by more than 25% compared with the control group(2.458×10~8 CFU / ml). | Tao ZHEN Wei CHEN Changqing SHA Xiancheng ZHANG Xinjie LIU Deshui YU | 2016 | Agricultural Biotechnology2016，5，4： | 0 |
| 6 | HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of barley immunity to powdery mildew fungus显示文摘Mitogen-activated protein kinase(MAPK)cascades play important roles in disease resistance in model plant species.However,the functions of MAPK signaling pathways in crop disease resistance are largely unknown.Here we report the function of HvMKK1-HvMPK4-HvWRKY1 module in barley immune system.HvMPK4 is identified to play a negative role in barley immune response against Bgh,as virus-induced gene silencing of HvMPK4 results in enhanced disease resistance whilst stably overexpressing HvMPK4 leads to super-susceptibility to Bgh infection.Furthermore,the barley MAPK kinase HvMKK1 is found to specifically interact with HvMPK4,and the activated HvMKK1^(DD) variant specifically phosphorylates HvMPK4 in vitro.Moreover,the transcription factor HvWRKY1 is identified to be a downstream target of HvMPK4 and phosphorylated by HvMPK4 in vitro in the presence of HvMKK1^(DD).Phosphorylation assay coupled with mutagenesis analyses identifies S122,T284,and S347 in HvWRKY1 as the major residues phosphorylated by HvMPK4.HvWRKY1 is phosphorylated in barley at the early stages of Bgh infection,which enhances its suppression on barley immunity likely due to enhanced DNA-binding and transcriptional repression activity.Our data suggest that the HvMKK1-HvMPK4 kinase pair acts upstream of HvWRKY1 to negatively regulate barley immunity against powdery mildew. | Pengya Xue Ling Zhang Renchun Fan Yanan Li Xinyun Han Ting Qi Lifang Zhao Deshui Yu Qian-Hua Shen | 2024 | Journal of Genetics and Genomics2024，51，3： | 0 |
| 7 | Characterization of β-1,4-D-mannanase Produced by Bacillus subtilis MSJ-5显示文摘Properties of enzyme production of a Bacillus subtitles strain( MSJ-5) isolated from soil were studied in the test. Enzyme production reached the peak when MSJ-5 being cultured in fermentation medium for 32 h. β-1,4-D-mannanase was the main component of crude enzyme solution,test results showed that the optimal reaction temperature of the enzyme was 50 ℃,the optimum reaction p H was 7. 0,and the enzyme was stable when p H was 5. 0- 7. 0. Hydrolysis test results showed that β-1,4-D-mannanase produced by MSJ-5 had significant viscosity reduction effects on Konjak mannan,and the major hydrolysis product was mannan oligosaccharide. The results indicated that β-1,4-D-mannanase produced by B. subtilis MSJ-5 had application potential in feed ingredients and functional oligosaccharides industry. | Wang Mei Shi Jing Tan Deshui Xu Yu Cui Rongzong Jiang Lihua | 2016 | Animal Husbandry and Feed Science2016，8，1： | 0 |
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