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| 1 | The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice显示文摘With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC | JIAO Demao, LI Xia, HUANG Xueqing, CHI Wei, KUANG Tingyun & Ku SB Maurice1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nangjing 210014, China 2. Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China 3. Botany Department, Washington State University, Pullman, WA 99164-4236, USA | 2001 | Chinese Science Bulletin2001，46，13： | 29 |
| 2 | Relationships between D1 protein, xanthophyll cycle and photodamage-resistant capacity in rice (Orysa sativa L.)显示文摘Relationships between D1 protein, xanthophyll cycle and subspecific difference of photodamage-resistant capacity have been studied in O. japonica rice varieties 02428 and 029 (photoinhibition-tolerance) and O. indica rice varieties 3037 and Palghar (photoinhibition-sensitivity) and their reciprocal cross F, hybrids after photoinhibitory treatment. It was shown that PS II photochemical efficiency (Fv /Fm) decreased, and xanthophyll cycle from violaxanthin (V), via anaxanthin (A), to zeaxanthin (Z) was enhanced and non-photochemical quenching (g/v) increased accordingly in SM-pretreated leaves of rice when the synthesis of D1 protein was inhibited, and that there was a decrease in qN and, as a result, more loss of D1 protein and a big decrease in Fv/Fm in DTT-pretreated leaves when xanthophyll cycle was inhibited. O. japonica subspecies had a higher maintaining capacity of D1 protein and a decrease of Fv /Fm in a more narrow range, and exhibited more resistance against photodamage, as compared with O. | Jl Benhua & JIAO Demao1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China 2. Department of Biology , Nantong Teachers College, Nantong 226007, China Correspondence should be addressed to Jiao Demao | 2000 | Chinese Science Bulletin2000，45，17： | 6 |
| 3 | Transforming growth factor-β1-induced N-cadherin drives cell–cell communication through connexin43 in osteoblast lineage显示文摘Gap junction(GJ)has been indicated to have an intimate correlation with adhesion junction.However,the direct interaction between them partially remains elusive.In the current study,we aimed to elucidate the role of N-cadherin,one of the core components in adhesion junction,in mediating connexin 43,one of the functional constituents in gap junction,via transforming growth factor-β1(TGF-β1)induction in osteoblasts.We first elucidated the expressions of N-cadherin induced by TGF-β1 and also confirmed the upregulation of Cx43,and the enhancement of functional gap junctional intercellular communication(GJIC)triggered by TGF-β1 in both primary osteoblasts and MC3T3 cell line.Colocalization analysis and Co-IP experimentation showed that N-cadherin interacts with Cx43 at the site of cell–cell contact.Knockdown of N-cadherin by siRNA interference decreased the Cx43 expression and abolished the promoting effect of TGF-β1 on Cx43.Functional GJICs in living primary osteoblasts and MC3T3 cell line were also reduced.TGF-β1-induced increase in N-cadherin and Cx43 was via Smad3 activation,whereas knockdown of Smad3 signaling by using siRNA decreased the expressions of both N-cadherin and Cx43.Overall,these data indicate the direct interactions between N-cadherin and Cx43,and reveal the intervention of adhesion junction in functional gap junction in living osteoblasts. | Yueyi Yang Wenjing Liu JieYa Wei Yujia Cui Demao Zhang Jing Xie | 2021 | International Journal of Oral Science2021，13，2： | 4 |
| 4 | Runxl protects against the pathological progression of osteoarthritis显示文摘Runt-related transcription factor-1(Runxl)is required for chondrocyte-to-osteoblast lineage commitment by enhancing both chondrogenesis and osteogenesis during vertebrate development.However,the potential role of Runxl in joint diseases is not well known.In the current study,we aimed to explore the role of Runxl in osteoarthritis induced by anterior cruciate ligament transaction(ACLT)surgery.We showed that chondrocyte-specific Runxl knockout(Runx1f/fCol2a1-Cre)aggravated cartilage destruction by accelerating the loss of proteoglycan and collagen II in early osteoarthritis.Moreover,we observed thinning and ossification of the growth plate,a decrease in chondrocyte proliferative capacity and the loss of bone matrix around the growth plate in late osteoarthritis.We overexpressed Runxl by adeno-associated virus(AAV)in articular cartilage and identified its protective effect by slowing the destruction of osteoarthritis in cartilage in early osteoarthritis and alleviating the pathological progression of growth plate cartilage in late osteoarthritis.ChIP-seq analysis identified new targets that interacted with Runxl in cartilage pathology,and we confirmed the direct interactions of these factors with Runxl by ChIP-qPCR.This study helps us to understand the function of Runxl in osteoarthritis and provides new clues for targeted osteoarthritis therapy. | Chenchen Zhou Yujia Cui Yueyi Yang Daimo Guo Demao Zhang Yi Fan Xiaobing Li Jing Zou Jing Xie | 2021 | Bone Research2021，9，4： | 4 |
| 5 | NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts显示文摘The adaptor protein NUMB is involved in asymmetric division and cell fate determination and recognized as an antagonist of Notch.Previous studies have proved that Notch activation in osteoblasts contributes to a high bone mass. In this study, however, an osteopenic phenotype was found in 9-week-old mice using osteoblastic specific Col1a1–2.3-Cre to ablate both Numb and its homologue Numbl. The trabecular bone mass decreased dramatically while the cortical bone mass was unaffected. Here, the Notch signal was not activated,while the tensin homologue deleted on human chromosome 10(PTEN), which dephosphorylates phosphatidylinositide 3-kinases, was elevated, attenuating protein kinase B(Akt). The ubiquitination assay revealed that NUMB may physiologically promote PTEN ubiquitination in the presence of neural precursor cell-expressed developmentally downregulated protein 4–1. In addition, the deficiency of Numb/Numbl also activated the Hedgehog pathway through GLI1. This process was found to improve the ratio of the receptor activator of nuclear factor-k B ligand to osteoprotegerin, which enhanced the differentiation of osteoclasts and bone resorption. In conclusion, this study provides an insight into new functons of NUMB and NUMBL on bone homeostasis. | Ling Ye Feng Lou Fanyuan Yu Demao Zhang Chenglin Wang Fanzi Wu Xin Li Yilin Ping Xiao Yang Jing Yang Dian Chen Bo Gao Dingming Huang Peng Liu | 2018 | Bone Research2018，6，4： | 3 |
| 6 | Compliant substratum modulates vinculin expression in focal adhesion plaques in skeletal cells显示文摘The biophysical properties of the extracellular matrix (ECM) dictate tissue-specific cell behaviour.In the skeleton system,bone shows the potential to adapt its architecture and contexture to environmental rigidity via the bone remodelling process,which involves chondrocytes,osteoblasts,osteoclasts,osteocytes and even peripheral bone marrow-derived stem/stromal cells (BMSCs).In the current study,we generated stiff (~1 014 ± 56) kPa,Young’s modulus) and soft (~46 ± 11) kPa silicon-based elastomer polydimethylsiloxane (PDMS) substrates by mixing curing agent into oligomeric base at 1:5 and 1:45 ratios,respectively,and investigated the influence of substrate stiffness on the cell behaviours by characterizing cell spreading area,cell cytoskeleton and cell adhesion capacity.The results showed that the cell spreading areas of chondrocytes,osteoblasts,osteoclasts,osteocytes and BMSCs were all reduced in the soft substrate relative to those in the stiff substrate.F-actin staining confirmed that the cytoskeleton was also changed in the soft group compared to that in the stiff group.Vinculin in focal adhesion plaques was significantly decreased in response to soft substrate compared to stiff substrate.This study establishes the potential correlation between microenvironmental mechanics and the skeletal system,and the results regarding changes in cell spreading area,cytoskeleton and cell adhesion further indicate the important role of biomechanics in the cell-matrix interaction. | Chenchen Zhou Qingxuan Wang Demao Zhang Linyi Cai Wei Du Jing Xie | 2019 | International Journal of Oral Science2019，11，2： | 3 |
| 7 | Osteoporosis-decreased extracellular matrix stiffness impairs connexin 43-mediated gap junction intercellular communication in osteocytes显示文摘Osteocytes are the main sensitive and responsive cells for mechanical stimuli in bone.The connexin family enables them to communicate with each other via forming functional gap junctions.However,how osteoporosis-impaired extracellular mechanical property modulates gap junction intercellular communication in osteocytes remains elusive.In this study,we established an ovariectomy(OVX)-induced osteoporosis mouse model in vivo and a polydimethylsiloxane(PDMS)-based cell culture substrate model in vitro to explore the influence of extracellular matrix(ECM)stiffness on cell-to-cell communication in osteocytes.Firstly,we established an OVX-induced osteoporosis mouse model by characterizing the changes in radiography,morphology and histochemistry of femurs.Our results showed that osteoporosis decreased the bone matrix stiffness together with the changes including the loss of osteocytes and the decrease of protein markers.Meanwhile,the dendritic process interconnection and channel-forming protein,Cx43,were reduced in osteoporosis mice.Next we mimicked ECM stiffness changes in vitro by using PDMS substrates at ratios 1:5 for normal stiffness and 1:45 for osteoporosis stiffness.Our results showed that the decreased ECM stiffness reduced the number of dendritic processes in a single cell and gap junctions between adjacent osteocytes.We further detected the decreased expression of Cx43,in the substrate with decreased stiffness.Finally,we found that gap junction-based intercellular communication was reduced in living osteocytes in the substrate with decreased stiffness.This study demonstrates the correlation between ECM mechanical property and cell-to-cell communication in osteocytes and might pave the way for further exploration of osteoporosis in terms of biomechanics. | Demao Zhang Xin Li Caixia Pi Linyi Cai Yang Liu Wei Du Wenbin Yang Jing Xie | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 3 |
| 8 | Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C4 photosynthesis enzymes显示文摘 | Demao Jiao Xueqing Huang Xia Li Wei Chi Tingyun Kuang Qide Zhang Maurice S. B. Ku Dongha Cho | 2002 | Photosynthesis Research2002，，1： | 1 |
| 9 | Relationships between sensitivities to chilling and high light and unsaturation of thylakoid membrane lipids in indica and japonica rice (Oryza sativa L)显示文摘 | Ji Benhua Jiao Demao | 2002 | Acta Botanica Scinca2002，44，2： | 1 |
| 10 | An Study of Smelting Reduction between V-Ti Slag System and Carbon Saturated Molten Iron显示文摘 | Tu Shiwei Wang Daguang Xuan Demao | 1989 | Iron Steel Vanadium Titanium1989，10，2： | 1 |
| 11 | The role of mechano growth factor in chondrocytes and cartilage defects:a concise review显示文摘Mechano growth factor(MGF),an isoform of insulin-like growth factor 1(IGF-1),is recognized as a typical mechanically sensitive growth factor and has been shown to play an indispensable role in the skeletal system.In the joint cavity,MGF is highly expressed in chondrocytes,especially in the damaged cartilage tissue caused by trauma or degenerative diseases such as osteoarthritis(OA).Cartilage is an extremely important component of joints because it functions as a shock absorber and load distributer at the weight-bearing interfaces in the joint cavity,but it can hardly be repaired once injured due to its lack of blood vessels,lymphatic vessels,and nerves.MGF has been proven to play an important role in chondrocyte behaviors,including cell proliferation,migration,differentiation,inflammatory reactions and apoptosis,in and around the injury site.Moreover,under the normalized mechanical microenvironment in the joint cavity,MGF can sense and respond to mechanical stimuli,regulate chondrocyte activity,and maintain the homeostasis of cartilage tissue.Recent reports continue to explain its effects on various cell types and sport-related tissues,but its role in cartilage development,homeostasis and disease occurrence is still controversial,and its internal biological mechanism is still elusive.In this review,we summarize recent discoveries on the role of MGF in chondrocytes and cartilage defects,including tissue repair at the macroscopic level and chondrocyte activities at the microcosmic level,and discuss the current state of research and potential gaps in knowledge. | Yi Liu Mengmeng Duan Demao Zhang Jing Xie | 2023 | Acta Biochimica et Biophysica Sinica2023，55，5： | 1 |
| 12 | Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C4 photosynthesis enzymes显示文摘 | Demao Jiao Xueqing Huang Xia Li Wei Chi Tingyun Kuang Qide Zhang Maurice S. B. Ku Dongha Cho | 2002 | Photosynthesis Research2002，，1： | 1 |
| 13 | Compression tests of high strength steel channel columns with interaction between local and distortional buckling 显示文摘 | Demao Yang Hancock G J | 2004 | Journal of Structural Engineering ASCE2004，130，12： | 1 |
| 14 | Study on early-stage development of conchospore in Porphyra yezoensis Ueda显示文摘 | FAN XIAOLEI WANG GUANGCE LI DEMAO | 2008 | Aquaculture2008，，278： | 1 |
| 15 | Compression Tests of High Strength Steel Channel Columns with Interaction between Local and Distortional Buckling显示文摘 | Demao Yang and Gregory J Hancock | 2004 | Journal of Structural Engineering ASCE2004，130，12： | 1 |
| 16 | Photochemical Efficiency of PSⅡand Membrane Lipid Peroxidation in Leaves of indica and japonica Rice(Oryza sativa)Under Chilling Temperature and Strong Light Stress Conditions显示文摘 | Ji Benhua Zhu Suqin Jiao Demao | 2002 | Acta Botanica Siniea2002，44，2： | 1 |
| 17 | Pyrolytic characteristics and kinetics of two brown algae and sodium alginate显示文摘 | Li Demao Chen Limei Yi Xiujie | 2010 | Bioresource Technology2010，101，18： | 1 |
| 18 | Compression Tests of High Strength Steel Channel Columns with Interaction Between Lo- cal and Distortional Buckling 显示文摘 | Yang Demao Hancock G J | 2004 | Journal of Structural Engi- neering2004，130，12： | 1 |
| 19 | Pyrolytic characteristics and kinetics of two brown algae and sodium alginate显示文摘 | Li Demao Chen Limei Yi Xiujie | 2010 | Bioresource Technology2010，101，18： | 1 |
| 20 | Compression tests of high strength steel channel columns with interaction between local and distortional buckling显示文摘 | Yang Demao Hancock G J | 2004 | Journal of Structural Engineering2004，130，2： | 1 |
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