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| 1 | Punicalagin ameliorates collagen-induced arthritis by downregulating M1 macrophage and pyroptosis via NF-κB signaling pathway显示文摘Rheumatoid arthritis(RA)is a chronic inflammatory disease that eventually leads to disability.Inflammatory cell infiltration,severe joint breaking and systemic bone loss are the main clinical symptoms.In this study,we established a collagen-induced arthritis(CIA)model and found a large number of M1 macrophages and pyroptosis,which are important sources of proinflammatory cytokines.Punicalagin(PUN)is an active substance extracted from pomegranate peel.We found that it inhibited joint inflammation,cartilage damage and systemic bone destruction in CIA mice.PUN effectively alleviated the high expression of inflammatory cytokines in synovial tissue in vivo.PUN treatment shifted macrophages from the M1 phenotype to the M2 phenotype after stimulation with lipopolysaccharide(LPS)and interferon(IFN)-γ.The expression of inducible nitric oxide synthase(i NOS)and other proinflammatory cytokines released by M1 macrophages was decreased in the PUN treatment group.However,simultaneously,the expression of markers of anti-inflammatory M2 macrophages,such as arginase(Arg)-1 and interleukin(IL)-10,was increased.In addition,PUN treatment attenuated pyroptosis by downregulating the expression of NLRP3 and caspase-1,thereby preventing inflammatory cell death resulting from the release of IL-1βand IL-18.Mechanistically,PUN inhibited the activation of receptor activators of the nuclear factor-κB(NF-κB)signaling pathway,which contributes to M1 polarization and pyroptosis of macrophages.We concluded that PUN ameliorated pathological inflammation by inhibiting M1 phenotype polarization and pyroptosis and has great potential as a therapeutic treatment for human RA. | Gaoran Ge Jiaxiang Bai Qing Wang Xiaolong Liang Huaqiang Tao Hao Chen Minggang Wei Junjie Niu Huilin Yang Yaozeng Xu Yuefeng Hao Yi Xue Dechun Geng | 2022 | Science China(Life Sciences)2022，65，3： | 14 |
| 2 | ROS signaling cascades:dual regulations for osteoclast and osteoblast显示文摘Accumulating evidence indicates that intracellular reactive oxygen species(ROS)production is highly involved in bone homeostasis by intervening osteoclast or osteoblast differentiation.Interestingly,ROS that are known as oxidizing agents exert dose-dependent biphasic properties in bone remodeling,including preventing osteoblast activity but accelerating osteoclast resorption.ROS mainly composed of superoxide anion radical,hydroxyl radical,nitric oxide,and two-electron reduction product hydrogen peroxide,which are important components to regulate bone cell metabolism and function in mammal skeleton.These free radicals can be partly produced in bone and boosted in an inflammation state.Although numerous researches have emphasized the impacts of ROS on bone cell biology and verified the mechanism of ROS signaling cascades,the recapitulatory commentary is necessary.In this review article,we particularly focus on the regulation of the intracellular ROS and its potential mechanism impacting on cell-signaling transduction in osteoclast and osteoblast differentiation for preferable understanding the pathogenesis and searching for novel therapeutic protocols for human bone diseases. | Huaqiang Tao Gaoran Ge Xiaolong Liang Weicheng Zhang Houyi Sun Meng Li Dechun Geng | 2020 | Acta Biochimica et Biophysica Sinica2020，52，10： | 8 |
| 3 | Protective effects of sirtuin 3 on titanium particle-induced osteogenic inhibition by regulating the NLRP3 inflammasome via the GSK-3β/β-catenin signalling pathway显示文摘Periprosthetic osteolysis(PPO)remains the key factor in implant failure and subsequent revision surgery and is mainly triggered by wear particles.Previous studies have shown that inhibition of osteoblastic differentiation is the most widespread incident affecting the interface of trabecular and loosening prostheses.Additionally,the NLRP3 inflammasome is activated by prosthetic particles.Sirtuin3,an NAD+-dependent deacetylase of mitochondria,regulates the function of mitochondria in diverse activities.However,whether SIRT3 can mitigate wear debris-induced osteolysis by inhibiting the NLRP3 inflammasome and enhancing osteogenesis has not been previously reported.Therefore,we investigated the role of SIRT3 during the process of titanium(Ti)particle-induced osteolysis.We revealed that upregulated SIRT3 dramatically attenuated Ti particle-induced osteogenic inhibition through suppression of the NLRP3 inflammasome and improvement of osteogenesis in vivo and in vitro.Moreover,we found that SIRT3 interference in the process of Ti particle-induced osteolysis relied on the GSK-3β/β-catenin signalling pathway.Collectively,these findings indicated that SIRT3 may serve as a rational new treatment against debris-induced PPO by deacetylase-dependent inflammasome attenuation. | Kai Zheng Jiaxiang Bai Ning Li Meng Li Houyi Sun Weicheng Zhang Gaoran Ge Xiaolong Liang Huaqiang Tao Yi Xue Yuefeng Hao Chen Zhu Yaozeng Xu Dechun Geng | 2021 | Bioactive Materials2021，6，10： | 5 |
| 4 | A new method of developing elastic-plastic-viscous constitutive model for clays显示文摘A new method is presented to develop the existing elastic-plastic constitutive model into an elastic-plastic-viscous one for clays.The actual loading process is divided into an instant process and a delayed process denoting the elastic-plastic strain and viscous strain, respectively. The elastic-plastic strain is determined by either an elastic-plastic model for overconsolidated clays or an improved model based on the elastic-plastic model for normally consolidated clays. In order to calculate viscous strain, a reference state line is defined based on the actual loading path. Combining the reference state line, an existing elastic-plastic model can be conveniently developed into an elastic-plastic-viscous model. Furthermore, using the proposed method, the modified cam clay model is extended into an elastic-plastic-viscous model. Comparisons with test results demonstrate that the extended model can capture the main time-dependent behaviours of clays, including creep, stress relaxation and strain rate effects. | LU DeChun MIAO JinBo DU XiuLi LIANG JingYu | 2020 | Science China(Technological Sciences)2020，63，2： | 3 |
| 5 | Enhanced anti-tumor immunity ex vivo induced by GM-CSF gene transducted dendritic cell vaccine显示文摘学习的目的是调查是否的目的树枝状的房间(DC ) 先锋,由 chemokine ligand 的注射招募了 3 (CCL3 ) ,在 granulocyte 巨噬细胞殖民地刺激以后导致提高的反肿瘤免疫在老鼠前 vivo 的因素(GM-CSF ) transfection。615 只老鼠经由尾巴静脉与 CCL3 被注射的方法。刚孤立的 B220-CD11c+ 房间与 cytokines 是有教养的。为 adenoviral (广告) 调停的基因 transduction, DC 是在感染(MOI ) 的复合的不同比率的转移 AdGM-CSF 基因决定最佳的基因 transfection 条件,并且在 transfection 以后检测 GM-CSF 的表示。GM-CSF 修改基因的 DC 的变化被词法观察,显型分析,和混合淋巴细胞反应(MLR ) 分析。DC 被装载,胃的癌症抗原由冻结并且融化的方法获得了。刺激 DC 种痘导致了 T 淋巴细胞,和 T 房间的杀死的效果到胃的癌症房间是由 MTT 的 assayed。INF- 生产与 INF- ELISA 工具包被决定。结果 B220-CD11c+ 房间数字在 CCL3 注射以后增加了。ELISA 结果证明在 GM-CSF 基因修正以后, DC 能生产 GM-CSF 的高水平。DC 是什么时候,在等于 1:100 的 MOI 转移了 AdGM-CSF 基因,在文化上层清液的 GM-CSF 水平到达了浸透[(130.00 ???????????????? ″ ? 匠牵楶楶? 僚 ??? 吗?? | Songbing He Liang Wang Kang Sun Yanyun Zhang Dechun Li | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，3： | 3 |
| 6 | Effect of silver growth temperature on the contacts between Ag and ZnO thin films显示文摘Highly c-axis oriented ZnO thin films were deposited on Si substrates by the pulsed laser deposition (PLD) method. At different growth temperatures,200 nm silver films as the contact metal were deposited on the ZnO thin films. The growth temperatures have great influence on the crystal quality of Ag films. Current-voltage characteristics were measured at room temperature. The Schottky contacts between Ag and ZnO thin films were successfully obtained when silver electrodes were deposited at 150 ℃ and 200℃. Ohmic contacts were formed while the growth temperatures were lower than 150℃ or higher than 200 ℃. After analysis,the forming of Ag/ZnO Schottky contacts was shown to be dependent on the appearance of the p-type inversion layer at the interface between Ag and ZnO layers. | LI XinKun LI QingShan LIANG DeChun XU YanDong XIE XiaoJun | 2009 | Science China(Technological Sciences)2009，52，9： | 2 |
| 7 | Rational integration of defense and repair synergy on PEEK osteoimplants via biomimetic peptide clicking strategy显示文摘Polyetheretherketone(PEEK)has been widely used as orthopedic and dental materials due to excellent mechanical and physicochemical tolerance.However,its biological inertness,poor osteoinduction,and weak antibacterial activity make the clinical applications in a dilemma.Inspired by the mussel adhesion mechanism,here we reported a biomimetic surface strategy for rational integration and optimization of anti-infectivity and osteo-inductivity onto PEEK surfaces using a mussel foot proteins(Mfps)-mimic peptide with clickable azido terminal.The peptide enables mussel-like adhesion on PEEK biomaterial surfaces,leaving azido groups for the further steps of biofunctionalizations.In this study,antimicrobial peptide(AMP)and osteogenic growth peptide(OGP)were bioorthogonally clicked on the azido-modified PEEK biomaterials to obtain a dual-effect of host defense and tissue repair.Since bioorthogonal clicking allows precise collocation between AMP and OGP through changing their feeding molar ratios,an optimal PEEK surface was finally obtained in this research,which could long-term inhibit bacterial growth,stabilize bone homeostasis and facilitate interfacial bone regeneration.In a word,this upgraded mussel surface strategy proposed in this study is promising for the surface bioengineering of inert medical implants,in particular,achieving rational integration of multiple biofunctions to match clinical requirements. | Meng Li Jiaxiang Bai Huaqiang Tao Li Hao Weiling Yin Xiaoxue Ren Ang Gao Ning Li Miao Wang Shiyuan Fang Yaozeng Xu Liang Chen Huilin Yang Huaiyu Wang Guoqing Pan Dechun Geng | 2022 | Bioactive Materials2022，7，2： | 2 |
| 8 | Research on the photoluminescence of spectral broadening by rapid thermal annealing on InAs/GaAs quantum dots显示文摘Photoluminescence (PL) test was conducted to investigate the effect of rapid thermal annealing (RTA) on the opticalperformance of self-assembled InAs/GaAs quantum dots (QDs) at the temperatures of 16 and 300 K. It was found that after RTAtreatment, the PL spectrum of the QDs sample had a large blue-shift and significantly broadened at 300 K. Compared with theas-grown InAs QDs sample, the PL spectral width has increased by 44.68 meV in the InAs QDs sample RTA-treated at800 ℃. The excitation power-dependent PL measurements showed that the broadening of the PL peaks of the RTA-treatedInAs QDs should be related to the emission of the ground state (GS) of different-sized InAs QDs, the InAs wetting layer (WL)and the In0.15Ga0.85As strain reduction layer (SRL) in the epitaxial InAs/GaAs layers. | Dandan Ning Yanan Chen Xinkun Li Dechun Liang Shufang Ma Peng Jin Zhanguo Wang | 2020 | Journal of Semiconductors2020，41，12： | 1 |
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