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| 1 | miR-182参与调节胶质母细胞瘤的凋亡、生长和分化(英文)显示文摘Glioblastoma multiforme(GBM)is a lethal,therapy-resistant brain cancer consisting of numerous tumor cell subpopulations,including stem-like glioma-initiating cells(GICs),which contribute to tumor recurrence following initial response to therapy.Here,we identified miR-182 as a regulator of apoptosis,growth,and differentiation programs whose expression level is correlated with GBM patient survival.Repression of Bcl2-like12(Bcl2L12),c-Met,and hypoxia-inducible factor 2α(HIF2A)is of central importance to miR-182 anti-tumor activity,as it results in enhanced therapy susceptibility,decreased GIC sphere size,expansion,and stemness in vitro.To evaluate the tumor-suppressive function of miR-182 in vivo,we synthesized miR-182-based spherical nucleic acids(182-SNAs);i.e.,gold nanoparticles covalently functionalized with mature miR-182 duplexes.Intravenously administered 182-SNAs penetrated the bloodbrain/blood-tumor barriers(BBB/BTB)in orthotopic GBM xenografts and selectively disseminated throughout extravascular glioma parenchyma,causing reduced tumor burden and increased animal survival.Our results indicate that harnessing the anti-tumor activities of miR-182 via safe and robust delivery of 182-SNAs represents a novel strategy for therapeutic intervention in GBM. | Kouri FM Hurley LA Daniel WL Day ES Hua Y Hao L Peng CY Merkel TJ Queisser MA Ritner C Zhang H James CD Sznajder JI Chin L Giljohann DA Kessler JA Peter ME Mirkin CA Stegh AH | 2015 | 中华神经外科疾病研究杂志2015，14，2： | 14 |
| 2 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 3 | Mild cognitive impairment:directions for future research显示文摘 | Luis CA Loewenstein DA Acevedo A | 2003 | Neurology2003，61，4： | 1 |
| 4 | The cardiovascular dose-response efects of isoflurane alone and combined with butorphanol in the green iguana( Iguana iguana)显示文摘 | Mosley CA Dyson D Smith DA | 2004 | Vet Anaesth Analg2004，31，1： | 1 |
| 5 | Liver fibrosis: noninvasive diagnosis with double contrast material-enhanced MR imaging 显示文摘 | Aguirre DA Behling CA Alpert E | 2006 | Radiology2006，239，： | 1 |
| 6 | Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity 显示文摘 | Pope WH Bowman CA Russell DA | 2015 | eLife2015，4，06： | 1 |
| 7 | Thyroidhormone transport is disturbed in erythrocytes frompatients with chronic renal failure on hemodialysis显示文摘 | Rodrigues MC Santos GM da Silva CA | 2004 | Ren Fail2004，26，： | 1 |
| 8 | The Tre2(USP6)oncogene is a hominoid-specific gene显示文摘 | Paulding CA Ruvolo M Haber DA | 2003 | Proc Nail Acad Sci USA2003，100，5： | 1 |
| 9 | Greater tuberosity changes as revealed by radiography:lack of clinical usefulness in patients with rotator cuff disease显示文摘 | Rubin DA Britton CA | 1999 | AJR1999，172，： | 1 |
| 10 | Vaginai miaoprostal administration for cervical ripening and labor induction显示文摘 | Wing DA Gaffaney CA | 2006 | Clin Obstert Gynercol2006，43，9： | 1 |
| 11 | Clinical characteristics, aetiological fac- tors and long-term prognosis of myocardial infarction with an absolutely normal coronary angiogram;a 3-year follow-up study of 91 patients显示文摘 | Da CA Isaaz K Faure E | 2001 | Eur Heart J2001，22，16： | 1 |
| 12 | Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos显示文摘 | Rappolee DA Brenner CA Schultz R | 1988 | Science1988，241，4874： | 1 |
| 13 | Microarray-based CGH detects chromosomal mosaicism not revealed by conventional cytogenetics显示文摘 | Cheung SW Shaw CA Scott DA | 2007 | Am J Med Genet2007，143，15： | 1 |
| 14 | A new artificial nerve graft containing rolled Schwann cell monolayers 显示文摘 | Hadlock TA Sundback CA Hunter DA | 2001 | Microsurgery2001，3，： | 1 |
| 15 | The Src family of tyrosine ki nases is important foy embryonic stem cell self-renewal显示文摘 | Anneren C Cowan CA Melton DA | 2004 | J Biol Chem2004，279，31： | 1 |
| 16 | Effect of glucocorticoids on stem cell factor expression in human asthmatic bronchi显示文摘 | Da Silva CA BlayF Israel-Biet D | 2006 | Clinical and Experimental Allergy2006，36，3： | 1 |
| 17 | The relationship between insomnia and health-related quality of life in patients with chronic illness 显示文摘 | Katz DA McHorney CA | 2002 | J Fam Pract2002，51，3： | 1 |
| 18 | Impact of obesity on health-related quality of life in Patients with chronic illness 显示文摘 | Katz DA McHorney CA Atkinson RL | 2000 | Journal of General Internal Medicine2000，15，11： | 1 |
| 19 | Loss of endothelial protein C receptors links coagulation and inflammation to parasite sequestration in cerebral malaria in African children 显示文摘 | Moxon CA Wassmer SC Milner DA Jr | 2013 | Blood2013，122，5： | 1 |
| 20 | Community-ac- quired pneumonia on the intensive care unit: secondary analysis of 17,869 cases in the ICNARC Case Mix Programme Database 显示文摘 | Woodhead M Welch CA Harrison DA | 2006 | Crit Care2006，10，2： | 1 |
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