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| 1 | Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain:One of the predominant strains circulating in China from 2017 to 2021显示文摘Porcine epidemic diarrhea(PED)caused by porcine epidemic diarrhea virus(PEDV)is one of the most devastating diseases in the global pig industry due to its high mortality rate in piglets.Maternal vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV challenge.From 2017 to 2021,we collected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of PEDV.The result showed that about 52.15%(158/303)of the farms were positive for PEDV with an overall detection rate of 63.95%(564/882)of the samples.The S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis.A total of 71 PEDV strains(68.27%)sequenced in this study were clustered into the predominant G2c subgroup,while the newly-defined G2d strains(9.62%)were identified in three provinces of China.The NH-TA2020 strain of G2c subgroup was isolated and cultured,and its infection to piglets caused watery diarrhea within 24 h,indicating its strong pathogenicity.Oral administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in colostrum.The piglets fed by the gilts above were challenged with NH-TA2020 strain or CH-HeB-RY-2020 strain from G2d subgroup,and the clinical symptoms and virus shedding were significantly reduced compared to the mock group.Our findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to 2021.Oral administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses,which confer protection against the homologous and emerging G2d PEDV strains challenges in neonates. | Xiaowen Li Yang Li Jiapei Huang Yali Yao Wenying Zhao Yunjing Zhang Jie Qing Jing Ren Zhong Yan Zewei Wang Xiaofang Hu Duli Kang Hongqiang Liu Zhichun Yan | 2022 | Virologica Sinica2022，37，5： | 6 |
| 2 | Osteopontin is an important mediator of alcoholic liver disease via hepatic stellate cell activation显示文摘AIM: To investigate over-expression of Osteopontin(OPN) pathway expression and mechanisms of action in human alcoholic liver disease(ALD), in vivo and in vitro acute alcohol models. METHODS: OPN pathway was evaluated in livers from patients with progressive stages of human ALD and serum from drinkers with and without liver cirrhosis. In vitro stellate LX2 cells exposed to acute alcohol and in vivo in acute alcoholic steatosis mouse models were also investigated for OPN pathway expression and function. WT and OPN-/- mice were administered an acute dose of alcohol and extent of liver injury was examined by histopathology and liver biochemistry after 16-24 h. The causative role of OPN was studied in OPN knockout animals and in vitro in stellate LX2 cells, utilizing siRNA, aptamer and neutralizing antibodies to block OPN and OPN pathway. OPN pathway expression and downstream functional consequences were measured for signaling by Western blotting, plasmin activation by spectrophotometric assays and cell migration by confocal imaging and quantitation. RESULTS: OPN expression positively correlated with disease severity in patients with progressive stages of ALD. In vivo, associated with alcoholic steatosis, a single dose of acute alcohol significantly increased hepatic OPN mRNA and protein, and a cleaved OPN form in a dose dependent manner. OPN mRNA and secreted OPN also increased in parallel with activation of LX2 stellate cells within 4 h of a single dose of alcohol. Expression of OPN receptors, αvβ3-integrin and CD44, increased in human ALD, and in vivo and in vitro with alcohol administration. This was accompanied by downstream phosphorylation of Akt and Erk, increased mRNA expression of several fibrogenesis, fibrinolysis and extracellular matrix pathway genes, plasmin activation and hepatic stellate cell(HSC) migration. Inhibition of OPN and OPN-receptor mediated signaling partially inhibited alcohol-induced HSC activation, plasmin activity and cell migration. CONCLUSION: OPN is a key mediator of the alcoholinduced effects on hepatic stellate cell functions and liver fibrogenesis. | Devanshi Seth Alastair Duly Paul C Kuo Geoffrey W McCaughan Paul S Haber | 2014 | World Journal of Gastroenterology2014，20，36： | 5 |
| 3 | Optical properties and chemical composition of the atmospheric aerosol in urban Guangzhou, China显示文摘 | Meinrat O. Andreae Otmar Schmid Hong Yang Duli Chand Jian Zhen Yu Li-Min Zeng Yuan-Hang Zhang | 2008 | Atmospheric Environment2008，，25： | 2 |
| 4 | Development of Precipitation Hardening Cr-Mo-Co Stainless Steel显示文摘 | Kasak A Chandhok C k Dulis E J | 1963 | Trans ASM1963，56，： | 1 |
| 5 | On the Competition Between Continuous and Discontinuous Precipitations in Binary Mg-Al Alloys显示文摘 | Duly D Simon J P Brechet Y | 1995 | Acta Metallurgic Materialia1995，43，1： | 1 |
| 6 | High-resolution electromicroscopy observation of the structure for continuous precipitates in an Mg-Al alloy and interpretation with O -lattice theory显示文摘 | Duly D Zhang W-Z Audier M | 1995 | Philos Mag1995，71，： | 1 |
| 7 | Influence of potassium deficiency on photosynthesis,chlorophyll contentand chloroplast ultra structure of cotton plants显示文摘 | ZHAO Duli D M OOSTERHUIS C W BEDNARZ | | 0，，01： | 1 |
| 8 | On the competion between continuous and discontinuous precipitation in binary Mg-Al alloys显示文摘 | DULY D SIMON J P BRECHET Y | 1995 | Acta Metallurgica et Materialia1995，43，1： | 1 |
| 9 | On the competition between continuous and discontinuous precipitation in binary Mg-Al alloy显示文摘 | DULY D SIMON J P Brechet Y | 1994 | Acta Metall Mater1994，43，1： | 1 |
| 10 | CA125 in ovarian can cer : Europe - an Group on Tumor Markers guidelines for clinical use显示文摘 | Duly MJ Bonfrer JM Kulpa J | 2005 | Int J Gynecol Cancer2005，15，5： | 1 |
| 11 | Nucleation mechanism of discontinuous precipitation in Mg-Al alloys and relation with the morphology显示文摘 | DULY D BRECHET Y | 1994 | Acta Metallurgica et Materialia1994，42，8： | 1 |
| 12 | High-resolution electron microscopy observations of the interface structure of continuous precipitates in a Mg-Al alloy and interpretation with the O-lattice theory显示文摘 | Duly D Zhang W Z Audier M | | 0，，： | 1 |
| 13 | On the competition between continuous and discontinuous precipitations in binary Mg-Al alloys显示文摘 | Duly D Simon J P Brechet Y | | 0，，： | 1 |
| 14 | Nucleation mechanism of discontinuous precipitation in Mg-Al alloys and relation with the morphology显示文摘 | DULY D BRECHET Y | 1994 | Acta Mater1994，42，： | 1 |
| 15 | On the competition between continuous and discontinuous precipitations in binary Mg-Al alloys显示文摘 | Duly D Simon J Brechet Y | 1995 | Acta Metall Mater1995，43，1： | 1 |
| 16 | Optical properties and chemical composition of the atmospheric aerosol in urban Guangzhou, China显示文摘 | Meinrat O. Andreae Otmar Schmid Hong Yang Duli Chand Jian Zhen Yu Li-Min Zeng Yuan-Hang Zhang | 2008 | Atmospheric Environment2008，，25： | 1 |
| 17 | On the competition between continuous and discontinuous precipitations in binary Mg-Al alloys显示文摘 | DULY D SIMON J P BRECHET Y | 1995 | Acta Metall Mater1995，43，1： | 1 |
| 18 | Early experience alters brain function and structure显示文摘 | Als H Duly FH McAnuky GB | 2004 | Pediatrics2004，113，4： | 1 |
| 19 | Directional modulation technique for phased arrays显示文摘 | Duly M Bernhard J | 2009 | IEEE Transactions on Antennas Propaga- tion2009，57，9： | 1 |
| 20 | Demonstration of direc- tional modulation using a phased array 显示文摘 | Daly M P Duly E L Bernhard J T | 2010 | IEEE Transac- tions on Antennas and Propagation2010，58，5： | 1 |
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