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1Rice Expression Database(RED):An integrated RNA-Seq-derived gene expression database for rice显示文摘Rice is one of the most important stable food as well as a monocotyledonous model organism for the plant research community.Here,we present RED(Rice Expression Database;http://gffzzd1bc3ab4bbbc4005hkwk0q50cccq06wfx.ffgz.tsg.suse.edu.cn),an integrated database of rice gene expression profiles derived entirely from RNA-Seq data.RED features a comprehensive collection of 284 high-quality RNA-Seq experiments,integrates a large number of gene expression profiles and covers a wide range of rice growth stages as well as various treatments.Based on massive expression profiles,RED provides a list of housekeeping and tissue-specific genes and dynamically constructs co-expression networks for gene(s) of interest.Besides,it provides user-friendly web interfaces for querying,browsing and visualizing expression profiles of concerned genes.Together,as a core resource in BIG Data Center,RED bears great utility for characterizing the function of rice genes and better understanding important biological processes and mechanisms underlying complex agronomic traits in rice.Lin Xia Dong Zou Jian Sang Xingjian Xu Hongyan Yin Mengwei Li Shuangyang Wu Songnian Hu Lili Hao Zhang Zhang 2017Journal of Genetics and Genomics2017,44,5:12
2Inositol Pyrophosphate lnsP8 Acts as an Intracellular Phosphate Signal in Arabidopsis显示文摘The maintenance of cellular phosphate(Pi)homeostasis is of great importance in living organisms.The SPX domain-containing protein 1(SPX1)proteins from both Arabidopsis and rice have been proposed to act as sensors of Pi status.The molecular signal indicating the cellular Pi status and regulating Pi homeostasis in plants,however,remains to be identified,as Pi itself does not bind to the SPX domain.Here,we report the identification of the inositol pyrophosphate lnsP8 as a signaling molecule that regulates Pi homeostasis in Arabidopsis.Polyacrylamide gel electrophoresis profiling of InsPs revealed that lnsP8 level positively cor­relates with cellular Pi concentration.We demonstrated that the homologs of diphosphoinositol pentaki-sphosphate kinase(PPIP5K),VIH1 and VIH2,function redundantly to synthesize lnsP8,and that the vih1 vih2 double mutant overaccumulates Pi.SPX1 directly interacts with PHR1,the central regulator of Pi star­vation responses,to inhibit its function under Pi-replete conditions.However,this interaction is compro­mised in the vih1 vih2 double mutant,resulting in the constitutive induction of Pi starvation-induced genes,indicating that plant cells cannot sense cellular Pi status without lnsP8.Furthermore,we showed that lnsP8 could directly bind to the SPX domain of SPX1 and is essential for the interaction between SPX1 and PHR1.Collectively,our study suggests that lnsP8 is the intracellular Pi signaling molecule serving as the ligand of SPX1 for controlling Pi homeostasis in plants.Jinsong Dong Guojie Ma Liqian Sui Mengwei Wei Viswanathan Satheesh Ruyue Zhang Shenghong Ge Jinkai Li Tong-En Zhang Christopher Wittwer Henning JJessen Huiming Zhang Guo-Yong An Dai-Yin Chao Dong Liu Mingguang Lei 2019Molecular Plant2019,12,11:8
3The Global Landscape of SARS-CoV-2 Genomes, Variants, and Haplotypes in 2019nCoVR显示文摘On January 22,2020,China National Center for Bioinformation(CNCB)released the 2019 Novel Coronavirus Resource(2019nCoVR),an open-access information resource for the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).2019nCoVR features a comprehensive integration of sequence and clinical information for all publicly available SARS-CoV-2 isolates,which are manually curated with value-added annotations and quality evaluated by an automated in-house pipeline.Of particular note,2019nCoVR offers systematic analyses to generate a dynamic landscape of SARS-CoV-2 genomic variations at a global scale.It provides all identified variants and their detailed statistics for each virus isolate,and congregates the quality score,functional annotation,and population frequency for each variant.Spatiotemporal change for each variant can be visualized and historical viral haplotype network maps for the course of the outbreak are also generated based on all complete and high-quality genomes available.Moreover,2019nCoVR provides a full collection of SARS-CoV-2 relevant literature on the coronavirus disease 2019(COVID-19),including published papers from PubMed as well as preprints from services such as bioRxiv and medRxiv through Europe PMC.Furthermore,by linking with relevant databases in CNCB,2019nCoVR offers data submission services for raw sequence reads and assembled genomes,and data sharing with NCBI.Collectively,SARS-CoV-2 is updated daily to collect the latest information on genome sequences,variants,haplotypes,and literature for a timely reflection,making 2019nCoVR a valuable resource for the global research community.2019nCoVR is accessible at http://gffzzdbc7b6aaae734bddskwk0q50cccq06wfx.ffgz.tsg.suse.edu.cn/ncov/.Shuhui Song Lina Ma Dong Zou Dongmei Tian Cuiping Li Junwei Zhu Meili Chen Anke Wang Yingke Ma Mengwei Li Xufei Teng Ying Cui Guangya Duan Mochen Zhang Tong Jin Chengmin Shi Zhenglin Du Yadong Zhang Chuandong Liu Rujiao Li Jingyao Zeng Lili Hao Shuai Jiang Hua Chen Dali Han Jingfa Xiao Zhang Zhang Wenming Zhao Yongbiao Xue Yiming Bao 2020Genomics, Proteomics & Bioinformatics2020,18,6:4
4Powder Quartz/Nano-TiO2 Composite: Mechanochemical Preparation and Photocatalytic Degradation of Formaldehyde显示文摘Powder quartz(PQ)/nano-TiO_2 composite was prepared by a mechanochemical method. Based on as-prepared PQ/nano-TiO_2 composite, we prepared interior paints and investigated the degradation efficiency of formaldehyde(DEF). Scanning electron microscopy showed that nano-TiO_2 got well dispersed by the adding of PQ. Thermogravimetric analysis indicated that the mass ratio of 4:1 was a relatively good proportion for the most production of PQ/nano-TiO_2 composite. Fourier transform-infrared spectrometry showed that the peak position of Ti-O-Si bond varied with the milling time. At the early stage, no characteristic peak of Ti-O-Si bond was observed, while at the later stage, new peaks at 902 cm^(-1) and 937 cm^(-1) appeared. Meanwhile, PQ/nano-TiO_2 composite-based interior paint exhibited significant DEF of 96.3% compared to that consisting of sole nanoTiO_2 of 92.0% under visible light illumination. As an abundant mineral resource, PQ would make interior paints with HCHO purifying effect much more efficient and cheaper.汪彬彬 邓跃全 何平 DONG Faqin DONG Mengwei DAI Ke ZHENG Guangya TIAN Hanxu LI Guanghua 2018Journal of Wuhan University of Technology(Materials Science)2018,33,6:3
5Spatial variation in grain-size population of surface sediments from northern Bering Sea and western Arctic Ocean: Implications for provenance and depositional mechanisms显示文摘In general,sediments in nature comprise populations of various diameters.Accurate information regarding the sources and depositional mechanisms of the populations can be obtained through their temporal and spatial comparisons.In this study,the grain size distribution of surface sediments from the Bering Sea and western Arctic Ocean were fitted and partitioned into populations using a log-normal distribution function.The spatial variations in the populations indicate differences in their sources and deposition mechanisms.The sediments on most of the Bering Sea Shelf originated from the Yukon River,and were transported westward by waves and currents.However,the presence of a coarser population outside Anadyr Bay was the result of Anadyr River transport.Additionally,a northward transport trend of fine suspended particles was observed on the west side of the Bering Sea Shelf.The sediments in Hope Valley in the south Chukchi Sea also originated from the Yukon River.The coarser population on the central Chukchi Sea Shelf originated from coast of Alaska to the east,not the Yukon River,and was transported by sea ice and bottom brine water.The populations of sediments from the Chukchi Basin and the base of the Chukchi Sea Slope are the result of sea ice and eddy action.Surface sediments from the western high Arctic Ocean predominantly comprised five populations,and two unique populations with mode diameters of 50–90μm and 200–400μm,respectively,were ubiquitous in the glacial and interglacial sediments.It was difficult to distinguish whether these two populations originated from sea ice or icebergs.Therefore,caution should be exercised when using either the>63μm or>250μm fractions in sediments as a proxy index for iceberg and ice sheet variation in the high Arctic Ocean.WANG Weiguo YANG Jichao ZHAO Mengwei DONG Linsen JIANG Min HUANG Erhui 2020Advances in Polar Science2020,31,3:2
6Preliminary study of calibration system for PZT thin film micro force sensor 显示文摘CUI Yan DONG Weijie LIU Mengwei 2007Ferroelectrics2007,358,1:1
7Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea(Pisum sativum L.)显示文摘Pea(Pisum sativum L.)is an annual cool-season legume crop.Owing to its role in sustainable agriculture as both a rotation and a cash crop,its global market is expanding and increased production is urgently needed.For both technical and regulatory reasons,neither conventional nor transgenic breeding techniques can keep pace with the demand for increased production.In answer to this challenge,CRISPR/Cas9 genome editing technology has been gaining traction in plant biology and crop breeding in recent years.However,there are currently no reports of the successful application of the CRISPR/Cas9 genome editing technology in pea.We developed a transient transformation system of hairy roots,mediated by Agrobacterium rhizogenes strain K599,to validate the efficiency of a CRISPR/Cas9 system.Further optimization resulted in an efficient vector,PsU6.3-tRNA-PsPDS3-en35S-PsCas9.We used this optimized CRISPR/Cas9 system to edit the pea phytoene desaturase(PsPDS)gene,causing albinism,by Agrobacterium-mediated genetic transformation.This is the first report of successful generation of gene-edited pea plants by this route.Guan Li Rong Liu Rongfang Xu Rajeev KVarshney Hanfeng Ding Mengwei Li Xin Yan Shuxian Huang Juan Li Dong Wang Yishan Ji Chenyu Wang Junguang He Yingfeng Luo Shenghan Gao Pengcheng Wei Xuxiao Zong Tao Yang 2023The Crop Journal2023,11,1:0
8Description of a new species in the genus Nephrotoma(Diptera:Tipuloidea:Tipulidae)from China,with redescriptions of hypopygiums of six species显示文摘A species of the genus Nephrotoma from Xizang,China is described as new to science,namely N.zhuolonggouensis Men,sp.nov.,which differs from its relative species by the structures of outer gonostylus,median lobe of sternite eighth and gonapophyses.Hypopygiums of another six species,N.concava Yang&Yang,1990,N.geniculata Yang&Yang,1987,N.hirsuticauda Alexander,1924,N.martynovi Alexander,1935,N.nigrostylata Alexander,1935 and N.virgata(Coquillett,1898),are redescribed based on additional characters.Qiulei Men Dong Yu Mengwei Sun 2023Zoological Systematics2023,48,3:0
9Freeze-drying assisted liquid exfoliation of BiFeO_(3) for pressure sensing显示文摘Breaking up bulk crystals of functional materials into nanoscale thinner layers can lead to interesting properties and enhanced functionalities due to the size and interface effects.However,unlike the van der Waals layered crystals,many materials cannot be exfoliated into thin layers by liquid exfoliation.BiFeO_(3) is a piezoelectric ceramic material,which is commonly synthesized as bulk crystals,limiting its wider applications.In this contribution,a freeze-drying assisted liquid exfoliation method was adopted to fabricate thin-layered BiFeO_(3) nanoplates with lateral sizes of up to 500 nm and thicknesses of 10−20 nm.The freeze-drying process showed a vital role in the preparation process by imposing stress on the dispersed BiFeO_(3) crystals during the liquid-to-solid-to-gas transition of the solvent.Such stress resulted in lattice strains in the freeze-dried BiFeO_(3) crystals,which enabled their further exfoliation under subsequent ultrasonication.Considering the intrinsic piezoelectric effect of BiFeO_(3),pressure sensors based on bulk and thin-layer BiFeO_(3) were also fabricated.The pressure sensor based on BiFeO_(3) nanoplates exhibited a largely enhanced sensitivity with a wider working range than the bulk counterpart,because of the stronger piezoelectric effect induced and the extra electrical charges at abundant interlayer interfaces.We suggest that the freeze-drying assisted liquid exfoliation method can be applied to other non-van der Waals crystals to bring about more functional material systems.Yuping Li Mengwei Dong Xuejie Zou Jinhao Zhang Jian Zhang Xiao Huang 2023Frontiers of physics2023,18,6:0
10Chromosome-scale genome sequence of Suaeda glauca sheds light on salt stress tolerance in halophytes显示文摘Soil salinity is a growing concern for global crop production and the sustainable development of humanity.Therefore,it is crucial to comprehend salt tolerance mechanisms and identify salt-tolerance genes to enhance crop tolerance to salt stress.Suaeda glauca,a halophyte species well adapted to the seawater environment,possesses a unique ability to absorb and retain high salt concentrations within its cells,particularly in its leaves,suggesting the presence of a distinct mechanism for salt tolerance.In this study,we performed de novo sequencing of the S.glauca genome.The genome has a size of 1.02 Gb(consisting of two sets of haplotypes)and contains 54761 annotated genes,including alleles and repeats.Comparative genomic analysis revealed a strong synteny between the genomes of S.glauca and Beta vulgaris.Of the S.glauca genome,70.56%comprises repeat sequences,with retroelements being the most abundant.Leveraging the allele-aware assembly of the S.glauca genome,we investigated genome-wide allele-specific expression in the analyzed samples.The results indicated that the diversity in promoter sequences might contribute to consistent allele-specific expression.Moreover,a systematic analysis of the ABCE gene families shed light on the formation of S.glauca’s flower morphology,suggesting that dysfunction of A-class genes is responsible for the absence of petals in S.glauca.Gene family expansion analysis demonstrated significant enrichment of Gene Ontology(GO)terms associated with DNA repair,chromosome stability,DNA demethylation,cation binding,and red/far-red light signaling pathways in the co-expanded gene families of S.glauca and S.aralocaspica,in comparison with glycophytic species within the chenopodium family.Time-course transcriptome analysis under salt treatments revealed detailed responses of S.glauca to salt tolerance,and the enrichment of the transition-upregulated genes in the leaves associated with DNA repair and chromosome stability,lipid biosynthetic process,and isoprenoid metabolic process.Additionally,genome-wide analysis of transcription factors indicated a significant expansion of FAR1 gene family.However,further investigation is needed to determine the exact role of the FAR1 gene family in salt tolerance in S.glauca.Yan Cheng Jin Sun Mengwei Jiang Ziqiang Luo Yu Wang Yanhui Liu Weiming Li Bing Hu Chunxing Dong Kangzhuo Ye Zixian Li Fang Deng Lulu Wang Ling Cao Shijiang Cao Chenglang Pan Ping Zheng Sheng Wang Mohammad Aslam Hong Wang Yuan Qin 2023Horticulture Research2023,10,9:0
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