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| 1 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 2 | Exercise training restores baroreflex sensitivity in never-treated hypertensive patients显示文摘 | LATERZA M C DE MATOS L D TROMBETTA I C | 2007 | Hypertension2007，49，6： | 1 |
| 3 | Nonlinear Jaynes-Cummings dynamics of a trapped ion显示文摘 | VOGEL W dE Matos Filho R L | | 0，，05： | 1 |
| 4 | Reli- able teleportation in trappedions显示文摘 | Solano E Cesar C L de Matos Filho R L | 2001 | European Physi- cal Journal D2001，13，1： | 1 |
| 5 | Trypsininhibitor from Dimorphandra mollis seeds: purification andproperties显示文摘 | Macedo M L de Matos D G Manhado 0 L | 2000 | Phytochemistry2000，54，6： | 1 |
| 6 | Theoretical and experimental analysis of plasticity equations for porous metals显示文摘 | de Matos Dias A Schaeffer L | 1993 | Int J Mach Tools Manufact1993，33，： | 1 |
| 7 | Fiber Bragg Grating (FBG) Characterization and Shaping by Local Pressure显示文摘 | Torres P Valente L C G | 2001 | Journal of Lightwave Technology2001，19，8： | 1 |
| 8 | Micheal additions of primary and secondary amines to acrylonitrile catalyzed by lipases显示文摘 | de Souza Roma Matos L M C Goncalves K M | 2009 | TetrahedronLetters2009，50，： | 1 |
| 9 | Enhanced morbidity of pectoralis major myoeutaneous flap used for salvage after previously failed oncological treatment and un- successful reconstructive head and neck surgery显示文摘 | RIBEIRO SALLES VANNI (2 M DE MATOS L L FARO JUNIOR M P | 2012 | Scientific World Journal2012，2012，38： | 1 |
| 10 | 查看详情显示文摘 | B M Escher R L de Matos L Davidovich | | 0，，05： | 1 |
| 11 | Moist storage of Brazil nut seeds for improved germination and nursery management显示文摘 | Karen A Kainer Mary L Duryea Marlene de Matos Malavasi | 1999 | Forest Ecology and Management1999，116，： | 1 |
| 12 | GPR survey at Lapa do Santo archaeological site, Lagoa Santa karstic region, Minas Gerais state, Brazil显示文摘 | Porsani J L Jangelme G de Matos Kipnis R | 2010 | Journal of Archaeo- logical Science2010，37，6： | 1 |
| 13 | Hepatopulmonary syndrome:case report and literature review显示文摘 | De Santis M Vaz Rodrigues L Matos F | 2010 | Revista portuguesa de pneumologia2010，16，4： | 1 |
| 14 | Prevalence of gestational diabetes mellitus-do the newWHO criteria make a differenceBrazilian Gestational DiabetesStudy Group显示文摘 | Schmidt MI Matos MC Reichelt AJ Forti AC de Lima L Dun-can BB | 2000 | Diabetes Med2000，17，5： | 1 |
| 15 | Crystal Growth and Characterization of a New Nonlinear Optical Material:Urea 1-Malic Acid显示文摘 | Dixit V K Vanishri S Bhata H L Matos Gomes E de Belsley M | 2003 | Journal of Crystal Growth2003，253，： | 1 |
| 16 | Comparison of four botulinum neurotoxin type A preparations in the treatment of hyperdynamic forehead lines in men: a pilot study 显示文摘 | Oliveira de Morais O Matos Reis-Filho E Vilela Pereira L | 2012 | J Drugs Dermatol2012，11，2： | 1 |
| 17 | Elective neck dissection versus observation in stage Ⅰ squamous cell carcinomas of the tongue and floor of the mouth显示文摘 | Dias F L Kligerman J Matos de Sa G | 2001 | Otolaryngol Head Neck Surg2001，125，1： | 1 |
| 18 | Serv- ice recovery paradox:a meta-analysis显示文摘 | DE MATOS C A HENRIQUE J L ROSSI C A V | 2007 | Journal of Serv- ice Research2007，10，1： | 1 |
| 19 | Fiber Bragg grating (FBG) characterization and shaping by local pressure 显示文摘 | DE MATOS C J S TORRES P VALENTE L C G | 2001 | J Lightwave Technol2001，19，： | 1 |
| 20 | Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil显示文摘 | Fernanda G. Braga Maria Lúcia M. Bouzada Rodrigo L. Fabri Magnum de O. Matos Francis O. Moreira Elita Scio Elaine S. Coimbra | 2006 | Journal of Ethnopharmacology2006，，2： | 1 |
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