| 1 | Sulindac sulfide selectively increases sensitivity of ABCC1 expressing tumor cells to doxorubicin and glutathione depletion显示文摘ATP-binding cassette(ABC) transporters ABCC1(MRP1),ABCB1(P-gp),and ABCG2(BCRP) contribute to chemotherapy failure.The primary goals of this study were to characterize the efficacy and mechanism of the nonsteroidal anti-inflammatory drug(NSAID),sulindac sulfide,to reverse ABCC1 mediated resistance to chemotherapeutic drugs and to determine if sulindac sulfide can influence sensitivity to chemotherapeutic drugs independently of drug efflux.Cytotoxicity assays were performed to measure resistance of ABC-expressing cell lines to doxorubicin and other chemotherapeutic drugs.NSAIDs were tested for the ability to restore sensitivity to resistance selected tumor cell lines,as well as a large panel of standard tumor cell lines.Other experiments characterized the mechanism by which sulindac sulfide inhibits ABCC1 substrate and co-substrate(GSH) transport in isolated membrane vesicles and intact cells.Selective reversal of multi-drug resistance(MDR),decreased efflux of doxorubicin,and fluorescent substrates were demonstrated by sulindac sulfide and a related NSAID,indomethacin,in resistance selected and engineered cell lines expressing ABCC 1,but not ABCB 1 or ABCG2.Sulindac sulfide also inhibited transport of leukotriene C_4 into membrane vesicles.Sulindac sulfide enhanced the sensitivity to doxorubicin in 24 of 47 tumor cell lines,including all melanoma lines tested(7-7).Sulindac sulfide also decreased intracellular GSH in ABCC1 expressing cells,while the glutathione synthesis inhibitor,BSO,selectively increased sensitivity to sulindac sulfide induced cytotoxicity.Sulindac sulfide potently and selectively reverses ABCC1-mediated MDR at clinically achievable concentrations.ABCC1 expressing tumors may be highly sensitive to the direct cytotoxicity of sulindac sulfide,and in combination with chemotherapeutic drugs that induce oxidative stress. | Jason D.Whitt Adam B.Keeton Bernard D.Gary Larry A.Sklar Kamlesh Sodani Zhe-Sheng Chen Gary A.Piazza | 2016 | The Journal of Biomedical Research2016，30，2： | 2 |
| 5 | 用地震裂缝分析提高钻井成功率显示文摘自从美国怀俄明州派恩德尔(Pinedale)背斜有多口成功气并完成于它的致密砂岩储层之后,这里已成为一个重要的勘探区。目前,已可以使用在邻区约拿气田开发的新增产技术来开采这些气藏。这里的最高产量似乎都来自具有天然裂缝的储层,因此,如果有一种能识别天然张性裂缝的新技术,就会有助于今后的钻井成功。这种新技术叫做AVAZ。它可以利用长炮检距中地震振幅的方位角变化来识别裂缝。在Veritas公司所承担的多用户三维地震勘探中,已使用这种技术的改进形式识别了井间区的裂缝。其解释结果可以和派恩德尔背斜气藏的已知裂缝进行对比。 | D.Gary S.Boerner D.Todorovic—Marinic 张云霞(译) 朱起煌(校) | 2005 | 石油地质科技动态2005，，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员