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| 1 | Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion显示文摘Development of a novel in vivo lung perfusion(IVLP)procedure allows localized delivery of high-dose doxorubicin(DOX)for targeting residual micrometastatic disease in the lungs.However,DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window.A small dimension nitinol wire coated with a sorbent of biocompatible morphology(Bio-SPME)has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key metabolites.The in vivo Bio-SPME-IVLP experiments were performed on pig model over various(150 and 225 mg/m^(2))drug doses,and during human clinical trial.Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL(respectively)dose of DOX during a 3-h IVLP.In both pig and human cases,DOX tissue levels presented similar trends during IVLP.Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure.In addition to DOX levels,Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening,providing information about lung status during drug administration.Real-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach.Bio-SPME also extracted various endogenous molecules,thus providing a real-time snapshot of the physiology of the cells,which might assist in the tailoring of personalized treatment strategy. | Barbara Bojko Nikita Looby Mariola Olkowicz Anna Roszkowska Bogumiła Kupcewicz Pedro Reck dos Santos Khaled Ramadan Shaf Keshavjee Thomas K.Waddell German Goomez-Rios Marcos Tascon Krzysztof Gorynski Marcelo Cypel Janusz Pawliszyn | 2021 | Journal of Pharmaceutical Analysis2021，11，1： | 3 |
| 2 | The efficacy and safety of EMLAcream for awake fiberoptic endotracheal intubation显示文摘 | Larijani GE Cypel D Gratz I et a1 | 2000 | Anesth Analg2000，91，8： | 1 |
| 3 | Normothermic eaz vivo perfusion prevents lung injury compared to extended cold preservation for transplantation 显示文摘 | Cypel M Rubacha M Yeung J | 2009 | Am J Transplant2009，9，： | 1 |
| 4 | Technique for prolonged normothermic ez vivo lung perfusion显示文摘 | Cypel M Yeung JC Hirayama S | 2008 | J Heart Lung Transplant2008，27，： | 1 |
| 5 | NormothermicEx Vivo Perfusion Prevents Lung Injury Compared to Extended Cold Pres- ervation for Transplantation 显示文摘 | Cypel M Rubacha M Yeung J | 2009 | Am J Transplant2009，9，10： | 1 |
| 6 | Functional out- comes and quality of life after normothermic ex vivo lung perfusion lung transplantation显示文摘 | Tikkanen JM Cypel M Machuca TN | 2015 | J Heart Lung Transplant2015，34，4： | 1 |
| 7 | r vivo lung perfusion 显示文摘 | Cypel M Yeung JC Hirayama S et ah Technique for prolonged normothermic e | 2008 | J Heart Lung Transplant2008，27，： | 1 |
| 8 | The efficacy and safety of EMLA cream for awake fiberoptic endotracheal intubation 显示文摘 | Larijani GE Cypel D Gratz I | 2000 | Anesthesia and analgesia2000，91，4： | 1 |
| 9 | Transcriptional signatures in donor lungs from donation after cardiac death vs after brain death: a functional pathway analysis 显示文摘 | Kang CH Anraku M Cypel M | 2011 | J Heart Lung Trans- plant2011，30，3： | 1 |
| 10 | The efficacy and safety of EMLAcream of awake fiberoptic endotracheal intubation显示文摘 | Larijani GE Cypel D GratzI | | 0，，08： | 1 |
| 11 | Pilomatricoma:experience of the hospital for sick children显示文摘 | Simon Cypel TK Vijayasekaran V Somers GR | | 0，，03： | 1 |
| 12 | The efficacy and safety of ENAL cream for awake fiberoptic endor- tracheal intubation显示文摘 | Lariani CE Cypel D Gratz I | 2000 | Anesth Analg2000，91，4： | 1 |
| 13 | Metabolomic fingerprinting of porcine lung tissue during pre-clinical prolonged ex vivo lung perfusion using in vivo SPME coupled with LC-HRMS显示文摘Normothermic ex vivo lung perfusion(NEVLP)has emerged as a modernized organ preservation technique that allows for detailed assessment of donor lung function prior to transplantation.The main goal of this study was to identify potential biomarkers of lung function and/or injury during a prolonged(19 h)NEVLP procedure using in vivo solid-phase microextraction(SPME)technology followed by liquid chromatography-high resolution mass spectrometry(LC-HRMS).The use of minimally invasive in vivo SPME fibers for repeated sampling of biological tissue permits the monitoring and evaluation of biochemical changes and alterations in the metabolomic profile of the lung.These in vivo SPME fibers were directly introduced into the lung and were also used to extract metabolites(on-site SPME)from fresh perfusate samples collected alongside lung samplings.A subsequent goal of the study was to assess the feasibility of SPME as an in vivo method in metabolomics studies,in comparison to the traditional inlab metabolomics workflow.Several upregulated biochemical pathways involved in pro-and antiinflammatory responses,as well as lipid metabolism,were observed during extended lung perfusion,especially between the 11th and 12th hours of the procedure,in both lung and perfusate samples.However,several unstable and/or short-lived metabolites,such as neuroprostanes,have been extracted from lung tissue in vivo using SPME fibers.On-site monitoring of the metabolomic profiles of both lung tissues through in vivo SPME and perfusate samples on site throughout the prolonged NEVLP procedure can be effectively performed using in vivo SPME technology. | Nikita Looby Anna Roszkowska Aadil Ali Barbara Bojko Marcelo Cypel Janusz Pawliszyn | 2022 | Journal of Pharmaceutical Analysis2022，12，4： | 1 |
| 14 | The efficacy and safety of EMLA cream for awake fiberoptic endotracheal intubation 显示文摘 | LARIJANI GE CYPEL D GRATZ I | 2000 | Anesth Analg2000，91，4： | 1 |
| 15 | Technique for prolonged normothermic ex vivo lung perfusion 显示文摘 | Cypel M Yeung JC Hirayama S | 2008 | J Heart Lung Trans- plant2008，27，12： | 1 |
| 16 | Cardiopulmonary Bypass and Extracorporeal Life Support for Emergent Intraoperative Thoracic Situations显示文摘 | Machuca TN Cypel M Keshavjee S | 2015 | Thorac Surg Clin2015，25，3： | 1 |
| 17 | Extracorporeal life support as a bridge to lung transplantation 显示文摘 | Cypel M Keshavjee S | 2011 | Clin Chest Med2011，32，2： | 1 |
| 18 | Technique for Prolonged Normothennic Ex Vivo Lung Perfusion 显示文摘 | Cypel M Yeung JC Hirayama S | 2008 | J Heart Lung Transplant2008，27，13： | 1 |
| 19 | Pilomatricoma: Experience of The Hospital for Sick Children显示文摘 | Tatiana Karine Simon Cypel Vijith Vijayasekaran Gino R Somers | 2007 | The Canadian Journal of Plasuc Surgery2007，15，3： | 1 |
| 20 | Experience with the first 50 ex vivo lung perfusion in clinical transplantation 显示文摘 | Cypel M Yeung JC Machuca T | 2012 | J Thorac Cardiovasc Surg2012，144，5： | 1 |
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