|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Correlation of N-myc downstream-regulated gene 1 expression with clinical outcomes of colorectal cancer patients of different race/ethnicity显示文摘AIM: To evaluate the role of N-myc downstream-regulated gene 1 (NDRG1) expression in prognosis and survival of colorectal cancer patients with different ethnic backgrounds. METHODS: Because NDRG1 is a downstream target of p53 and hypoxia inducible factor-1α (HIF-1α),we examined NDRG1 expression together with p53 and HIF-1α by immunohistochemistry. A total of 157 colorectal cancer specimens including 80 from Japanese patients and 77 from US patients were examined. The correlation between protein expression with clinicopathological features and survival after surgery was analyzed.in colorectal tumor compared with normal epithelium in both Japanese and US patient groups. Expression of NDRG1 protein was significantly correlated with lymphatic invasion,venous invasion,depth of invasion,histopathological type,and Dukes' stage in Japanese colorectal cancer patients. NDRG1 expression was correlated to histopathological type,Dukes' stage and HIF-1α expression in US-Caucasian patients but not in US-African American patients. Interestingly,Kaplan-Meier survival analysis demonstrated that NDRG1 expression correlated significantly with poorer survival in US-African American patients but not in other patient groups. However,in p53-positive US cases,NDRG1 positivity correlated significantly with better survival. In addition,NDRG1 expression also correlated significantly with improved survival in US patients with stages Ⅲ and Ⅳ tumors without chemotherapy. In Japanese patients with stages Ⅱ and Ⅲ tumors,strong NDRG1 staining in p53-positive tumors correlated significantly with improved survival but negatively in patients without chemotherapy. CONCLUSION: NDRG1 expression was correlated with various clinicopathological features and clinical outcomes in colorectal cancer depending on the race/ethnicity of the patients. NDRG1 may serve as a biological basis for the disparity of clinical outcomes of colorectal cancer patients with different ethnic backgrounds. | Minori Koshiji Kensuke Kumamoto Keiichirou Morimura Yasufumi Utsumi Michiko Aizawa Masami Hoshino Shinji Ohki Seiichi Takenoshita Max Costa Thérèse Commes David Piquemal Curtis C Harris Kam-Meng Tchou-Wong | 2007 | World Journal of Gastroenterology2007，13，20： | 25 |
| 2 | Adjuvant chemotherapy for resected colorectal cancermetastases:literature review and meta-analysis显示文摘Surgical resection is the only option of cure for patients with metastatic colorectal cancer(CRC). However, the risk of recurrence within 18 mo after metastasectomy is around 75% and the liver is the most frequent site of relapse. The current international guidelines recommend an adjuvant therapy after surgical resection of CRC metastases despite the lower level of evidence(based on the quality of studies in this setting). However, there is still no standard treatment and the effective role of an adjuvant therapy remains controversial. The aim of this review is to report the state-of-art of systemic chemotherapy and regional chemotherapy with hepatic arterial infusion in the management of patients after resection of metastases from CRC, with a literature review and meta-analysis of the relevant randomized controlled trials. | Giovanni Brandi Stefania De Lorenzo Margherita Nannini Stefania Curti Marta Ottone Filippo Gustavo Dall’Olio Maria Aurelia Barbera Maria Abbondanza Pantaleo Guido Biasco | 2016 | World Journal of Gastroenterology2016，22，2： | 13 |
| 3 | Global Wheat Head Detection(GWHD)Dataset:A Large and Diverse Dataset of High-Resolution RGB-Labelled Images to Develop and Benchmark Wheat Head Detection Methods显示文摘The detection of wheat heads in plant images is an important task for estimating pertinent wheat traits including head population density and head characteristics such as health,size,maturity stage,and the presence of awns.Several studies have developed methods for wheat head detection from high-resolution RGB imagery based on machine learning algorithms.However,these methods have generally been calibrated and validated on limited datasets.High variability in observational conditions,genotypic differences,development stages,and head orientation makes wheat head detection a challenge for computer vision.Further,possible blurring due to motion or wind and overlap between heads for dense populations make this task even more complex.Through a joint international collaborative effort,we have built a large,diverse,and well-labelled dataset of wheat images,called the Global Wheat Head Detection(GWHD)dataset.It contains 4700 high-resolution RGB images and 190000 labelled wheat heads collected from several countries around the world at different growth stages with a wide range of genotypes.Guidelines for image acquisition,associating minimum metadata to respect FAIR principles,and consistent head labelling methods are proposed when developing new head detection datasets.The GWHD dataset is publicly available at http://gffzzef49fa24bca249bbh90bvww05k9ck66fu.ffgz.tsg.suse.edu.cn/and aimed at developing and benchmarking methods for wheat head detection. | Etienne David Simon Madec Pouria Sadeghi-Tehran Helge Aasen Bangyou Zheng Shouyang Liu Norbert Kirchgessner Goro Ishikawa Koichi Nagasawa Minhajul A.Badhon Curtis Pozniak Benoit de Solan Andreas Hund Scott C.Chapman Frédéric Baret Ian Stavness Wei Guo | 2020 | Plant Phenomics2020，2，1： | 13 |
| 4 | Evolving paradigms in biological carbon cycling in the ocean显示文摘Carbon is a keystone element in global biogeochemical cycles.It plays a fundamental role in biotic andabiotic processes in the ocean,which intertwine to mediate the chemistry and redox status of carbon in the ocean and the atmosphere.The interactions between abiotic and biogenic carbon(e.g.CO_2,Ca CO_3,organic matter)in the ocean are complex,and there is a half-century-old enigma about the existence of a huge reservoir of recalcitrant dissolved organic carbon(RDOC)that equates to the magnitude of the pool of atmospheric CO_2.The concepts of the biological carbon pump(BCP)and the microbial loop(ML)shaped our understanding of the marine carbon cycle.The more recent concept of the microbial carbon pump(MCP),which is closely connected to those of the BCP and the ML,explicitly considers thesignificance of the ocean’s RDOC reservoir and provides a mechanistic framework for the exploration of its formation and persistence.Understanding of the MCP has benefited from advanced‘omics’and novel research in biological oceanography and microbial biogeochemistry.The need to predict the ocean’sresponse to climate change makes an integrative understanding of the BCP,ML and MCP a high priority.In this review,we summarize and discuss progress since the proposal of the MCP in 2010 and formulate research questions for the future. | Chuanlun Zhang Hongyue Dang Farooq Azam Ronald Benner Louis Legendre Uta Passow Luca Polimene Carol Robinson Curtis A.Suttle Nianzhi Jiao | 2018 | National Science Review2018，5，4： | 12 |
| 5 | Lessons in bridge damage learned from the Wenchuan earthquake显示文摘A strong earthquake occurred in Wenchuan County,Sichuan Province,China,on May 12,2008.Shortly after the earthquake,the Turner-Fairbank Highway Research Center of the Federal Highway Administration,in partnership with the Research Institute of Highways,the Ministry of Communication of China,led a reconnaissance team to conduct a post-earthquake bridge performance investigation of the transportation system in the earthquake affected areas.The U.S.transportation system reconnaissance team visited the area during July 20-24,2008.This paper presents the fi ndings and lessons learned by the team. | W. Phillip Yen Genda Chen Mark Yashinski Youssef Hashash Curtis Holu Wang Kehai GuoXiaodong | 2009 | Earthquake Engineering and Engineering Vibration2009，8，2： | 11 |
| 6 | 云南湄公河流域上游河谷地区疟疾媒介传疟作用研究(英文)显示文摘目的通过观察蚊媒季节消长、嗜血习性、叮咬行为和疟疾子孢子率以研究出它们在云南湄公河上游河谷地带传疟作用的关系。方法村内采取诱蚊灯和人工诱捕方法捕蚊;现场采取显微镜解剖蚊虫的方法观察唾腺子孢子,实验室采用ELISA技术检测蚊虫体内环子孢子蛋白(CSP)。结果村内共捕获16种按蚊。现场显微镜解剖7种按蚊,1 010只经产蚊中,发现了7只唾液腺感染子孢子;ELISA方法检测现场捕回的8种按蚊,5 154只各龄期蚊中,发现11只环子孢子蛋白阳性蚊。微小按蚊、中华按蚊、多斑按蚊子孢子阳性率(包括ELISA环子孢子蛋白阳性和镜检唾腺子孢子感染蚊)分别是0.37%、0.22%、0.32%,昆虫接种率分别估算为9.82、3.97、2.69。这些蚊媒叮咬活动起始于黄昏,并整夜有活动,但微小按蚊夜间活动高峰出现于子夜,中华按蚊和多斑按蚊的高峰则在21:00之前。它们的人血指数都在80%以上。结论以上结果显示微小按蚊、中华按蚊和多斑按蚊是湄公河上游河谷地带的重要传疟媒介。 | 周红宁 张再兴 李春富 吴超 王丕玉 Chris Curtis Nigel Hill | 2005 | 中国寄生虫病防治杂志2005，18，6： | 11 |
| 7 | 心肺运动试验评价食管癌患者化疗后整体功能变化的临床研究显示文摘背景化疗对人体整体功能具有显著损害,但目前尚缺乏对化疗患者整体功能变化进行客观定量评估的可靠方法。目的探讨心肺运动试验(CPET)对食管癌患者化疗前后整体功能变化的评估作用。方法选取2006—2007年度在德州大学安德森癌症中心进行术前化疗的白种人男性食管癌患者6例,分别在化疗前后进行CPET检查,按照美国加州大学洛杉矶分校医学中心标准连续递增功率方案完成症状限制性极限运动,通过对数据标准化分析计算其核心指标,从系统软件导出静息状态、热身状态、无氧阈状态、极限状态时的循环指标及呼吸指标。结果 6例食管癌患者化疗后,除1例患者无氧阈略有升高外,其余患者的峰值摄氧量、无氧阈、峰值氧脉搏、摄氧效率平台、峰值负荷功率和递增功率运动时间均降低;化疗后,除1例患者的二氧化碳通气当量斜率略有降外,其余所有患者的二氧化碳通气当量斜率和二氧化碳通气当量最低值均升高。食管癌患者化疗前后无氧阈(L/min、ml·min-1·kg^(-1)、%pred)、二氧化碳通气当量斜率(slope、%pred)、递增功率运动时间(min)比较,差异均无统计学意义(P>0.05);化疗后峰值摄氧量(L/min、ml·min-1·kg^(-1)、%pred)、峰值氧脉搏(ml/次、%pred)、摄氧效率平台(比值、%pred)、峰值负荷功率(W)较化疗前降低,二氧化碳通气当量最低值(比值、%pred)较化疗前升高(P<0.05)。食管癌患者化疗后极限状态时摄氧量较化疗前降低(P<0.05);静息状态时心率较化疗前升高,收缩压较化疗前降低(P<0.05);热身状态时舒张压、平均动脉压较化疗前降低(P<0.05);4个状态时氧脉搏较化疗前降低(P<0.05);静息状态、热身状态、极限状态时摄氧通气效率较化疗前降低(P<0.05);极限状态时二氧化碳通气当量较化疗前升高(P<0.05)。食管癌患者化疗后热身状态时潮气末二氧化碳分压较化疗前降低(P<0.05);静息状态、热身状态、极限状态时潮气末氧分压较化疗前升高(P<0.05);极限状态时每呼吸摄氧量较化疗前降低(P<0.05);无氧阈状态、极限状态时每呼吸二氧化碳排出量较化疗前降低(P<0.05)。结论化疗使得食管癌患者循环和细胞代谢功能显著降低,而呼吸受限相对较轻,且处于相对代偿反应。CPET检查可以对食管癌患者化疗前的整体功能进行客观定量评估,同时也可以对化疗所造成的整体功能改变进行评估。 | 孙兴国 Curtis Hightower 刘方 William W.Stringer 葛万刚 | 2016 | 中国全科医学2016，19，17： | 11 |
| 8 | Nrf2抗氧化损伤通路在CCl_4所致小鼠肝毒性中的保护作用(英文)显示文摘目的观察核转录相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)抗氧化损伤通路对四氯化碳(CCl4)所致小鼠肝毒性的保护作用。方法选取Nrf2-null、Wild-type、Keap1-KD、Keap1-HKO小鼠,按基因型分为4组,分别给予40 mg/kg CCl4腹腔注射,腹腔注射生理盐水(10 m L/kg)作对照,16 h后收集血浆和肝组织样本,检测血中谷丙转氨酶(alanine transaminase,ALT)和乳酸脱氢酶(lactic dehydrogenase,LDH)活性以及肝组织中丙二醛(malondialdehyde,MDA)含量;HE染色观察肝组织病理学变化,Real-time PCR和Western blot法检测相关基因表达。结果 CCl4增加Nrf2-null组和Wild-type组血中ALT、LDH活性及肝组织MDA含量,同时造成肝组织病理损伤,而在Keap1-KD组和Keap1-HKO组影响不明显;Nrf2因子靶基因(Nqo1和Gclc)在CCl4的诱导表达逐渐增高(顺序为Nrf2-null、Wild-type、Keap1-KD、Keap1-HKO),继而使炎症因子、内质网应激基因、细胞凋亡基因、细胞坏死基因的表达(m KC、MIP-2、IL-1β、TNFα、Gadd45、Chop10、Bax、Caspase 3、Mcl、Noxa)按Nrf2-null、Wild-type、Keap1-KD、Keap1-HKO顺序逐渐下降。结论 Nrf2因子激活保护CCl4所致小鼠肝损伤反应。 | 陆远富 Connie Wu 刘杰 Curtis Dean Klaassen | 2015 | 遵义医学院学报2015，38，1： | 11 |
| 9 | 西双版纳热带森林挥发性有机物的观测研究显示文摘2002年7月中旬利用异戊二烯通量测定系统对云南西双版纳热带森林异戊二烯的排放通量进行了连续测量,同时对光合有效辐射(PAR)、气温、湿度、风等参数进行了观测.结果表明,热带地区人工橡胶林区域湿季异戊二烯的排放有明显的日变化规律,中午前后为一天中的最大值,异戊二烯排放通量的日平均值在0.85~0.24mgC/(m2(h)之间.对小时值和日平均值而言,异戊二烯的排放与PAR和气温的变化规律比较一致,但它对PAR比对气温更加敏感.不同的天气状况对异戊二烯的排放有非常明显的影响.橡胶林是一个单萜烯排放者,其单萜烯的排放速率明显依赖于PAR.橡胶林和热带雨林冠层上空异戊二烯和单萜烯的浓度也有明显的日变化规律,而且橡胶林区域的异戊二烯和单萜烯的浓度均明显高于热带雨林. | 白建辉 Bradly Baker Curtis Johnson 李庆军 王永峰 赵崇奖 Lee Klinger Alex Guenther Jim Greenberg | 2004 | 中国环境科学2004，24，2： | 9 |
| 10 | 地方城市活动走廊:一种真正整合土地使用和交通规划的有效方法?显示文摘本文的中心议题是探讨形成未来城市形态的途径,这种城市形态通过整合土地使用和交通规划可以有效提供可持续的交通。过去近50年来,在澳大利亚珀斯大城市地区推行的土地使用政策及其城市形态没能有效地实现上述目的。作者认为,“地方活动走廊规划”或许能够成为一种有效实现整合的规划方法。 | Carey Curtis 王金秋 | 2002 | 国外城市规划2002，17，6： | 8 |
| 11 | ELISA检测云南按蚊环子孢子蛋白的评价显示文摘目的 采用酶联免疫吸附测定 (ELISA)技术检测疟疾媒介按蚊环子孢子蛋白 (CSP) ,评价ELISA用于云南疟疾媒介按蚊传疟效能调查的可行性。 方法 现场捕获疟疾媒介按蚊经产蚊 ,每只蚊镜检 3叶唾腺子孢子感染率 ,ELISA检测另 3叶唾腺CSP ;用含间日疟原虫配子体的患者血人工喂饲微小按蚊 ,11d后同法镜检唾腺子孢子感染率及ELISA检测唾腺CSP。在种植场捕获 8种按蚊 (微小按蚊、中华按蚊、多斑按蚊、迷糊按蚊、可赫按蚊、带足按蚊、菲律宾按蚊和须喙按蚊 )各龄成蚊 ,ELISA检测唾腺CSP。 结果 ①现场捕获经产蚊 10 10只 ,镜检唾腺子孢子阳性 7只 ,阳性率为 0 69% ;ELISA检测唾腺CSP阳性 8只 ,阳性率为 0 79% ;其中 6只蚊两项检测均为阳性 ,阳性率为 0 5 9%。两法比较 ,差异无显著性意义 (P >0 0 5 )。②人工感染 3 6只微小按蚊 ,镜检唾腺子孢子阳性2 7只 ,阳性率为 75 0 % ;ELISA检测唾腺CSP阳性 2 9只 ,阳性率为 80 6% ;其中有 2 6只蚊两项检测 (Pv2 10CSP)均阳性 ,阳性率为 72 2 %。两法比较 ,差异无显著性意义 (P >0 0 5 )。③种植场捕获 8种按蚊各龄成蚊 4675只 ,ELISA检测唾腺CSP阳性 11只 ,阳性率为 0 2 4% ;其中Pv2 10阳性 9只 ,Pf2A10阳性 2只 ;微小按蚊。 | 周红宁 张再兴 Chris Curtis Nigel Hill 李春富 吴超 王丕玉 | 2004 | 中国寄生虫学与寄生虫病杂志2004，22，4： | 7 |
| 12 | Effects of Soil Fertility and Atmospheric CO_2 Enrichment on Leaf, Stem and Root Dark Respiration of Populus tremuloides显示文摘An open-top chamber experiment was conducted at the University of Michigan Biological Station near Pellston, Michigan, USA, to study the effects of soil fertility and CO2 on leaf, stem and root dark respiration (Rd) of Populus tremuloides. Overall, area-based day-time leaf Rd (Rda) was significantly greater at elevated than at ambient CO2 in high-fertility soil, but not in low-fertility soil. Mass-based leaf Rd (Rdm) was overall greater for high- than for low-fertility soil grown trees at elected, but not at ambient CO2. Nighttime leaf Rd. and Rdm were unthected by soil fertility or CO2, nor was stem Rda, which ranged from 1.0 to 1.4 μmol m-2 s-1 in the spring and 3.5 to 4.5 μmol m-2 s-1 in the summer. Root Rda. was significantly higher in high- than in low-fertility soil, but was unaffected by CO2. Since biomass production of P. tremuloides will be significantly greater at elevated CO2 while specific Rd will either increase or remain unchanged, we predict that carbon loss to the atmosphere through respiration from this ecologically important species would increase at higher CO2. Soil fertility would also interact with elevated CO2 in affecting the carbon flow in the plant-soil-air system. | X. Z. WANG P. S. CURTIS and C. S. VOGEL Columbia University, Palisades, New York 10964-8000 (USA) The Ohio State University, Columbus, Ohio 43210-1293 (USA) University of Michigan Biological Station, Pellston, Michigan 49769 (USA) | 2001 | Pedosphere2001，11，3： | 6 |
| 13 | Estimating underground mine ventilation friction factors from low density 3D data acquired by a moving LiDAR显示文摘Ventilation system analysis for underground mines has remained mostly unchanged since the Atkinson method was made popular by Mc Elroy in 1935. Data available to ventilation technicians and engineers is typically limited to the quantity of air moving through any given heading. Because computer-aided modelling, simulation, and ventilation system design tools have improved, it is now important to ensure that developed models have the most accurate information possible. This paper presents a new technique for estimating underground drift friction factors that works by processing 3 D point cloud data obtained by using a mobile Li DAR. Presented are field results that compare the proposed approach with previously published algorithms, as well as with manually acquired measurements. | Curtis Watson Joshua Marshall | 2018 | International Journal of Mining Science and Technology2018，28，4： | 6 |
| 14 | RNA-Seq Analysis of Developing Nasturtium Seeds (Tropaeolum majus): Identification and Characterization of an Additional Galactosyltransferase Involved in Xyloglucan Biosynthesis显示文摘一条深定序的途径被追求利用 454,定序方法发现新基因的 Illumina 在 xyloglucan 包含了生合成。cDNA 序列从开发旱金莲属植物(金莲花属之植物 majus ) 被产生作为种子存储聚合物播种 xyloglucan,它生产大量 non-fucosylated。除了已知的 xyloglucan biosynthetic 基因,一以前 uncharacterized 通常认为的 xyloglucan galactosyltransferase 被识别。在相应 ortholog (AT5G62220 ) 的一个 Arabidopsis thaliana 异种线有缺陷者的分析表明这基因不与以前描绘的 xyloglucan galactosyltransferase 显示出冗余性, MUR3,但是在一个不同位置为 xyloglucan 的 galactosyl 替换被要求。基因为 Xyloglucan L 方面链 galactosylTransferase 位置 2 被称为 XLT2。它在作为 MUR3 的 glycosyltransferase 家庭 47 的一样的 subclade 代表酶。在 MUR3 (mur3.1 ) 和 XLT2 的一个双变异的有缺陷者与由仅仅 xylosylated glucosyl 单位实质上组成的 xyloglucan 导致了 Arabidopsis 植物,没有进一步的替换。 | Jacob K. Jensen Alex Schultink Kenneth Keegstra Curtis G. Wilkerson Markus Pauly | 2012 | Molecular Plant2012，5，5： | 6 |
| 15 | 齐墩果醇酸对化学物质致小鼠急性肝损伤的保肝作用(英文)显示文摘目的:评价齐墩果醇酸(OA)对急性肝损伤的保肝作用.方法:小鼠sc OA 200 μmol·kg^(-1)三天,然后给予肝毒物.通过病理组织学观察及测定血清丙氨酸转氨酶和艾杜糖醇脱氢酶活性来估价肝损伤.结果:OA能明显减轻四氯化碳,溴苯,醋氨酚,速尿,硫代乙酰胺,鬼笔毒环肽,秋水仙硷,氯化镉,D—半乳糖胺和内毒素等所致小鼠急性坏死性肝损伤,降低这些肝毒物所引起的血清转氨酶和艾杜糖醇脱氢酶的升高,但对氯仿,二甲亚硝氨,鹅膏菌索和烯丙醇的毒性无作用.结论:OA能减轻多种化学物质(但并非全部)引起的肝损伤.其保肝机制可能是多方面的. | 刘杰 刘亚平 Curtis D KLAASSEN | 1995 | 中国药理学报1995，16，2： | 5 |
| 16 | Spouting behaviour of binary mixtures in square-based spouted beds显示文摘From experime nts, the influe nee of the physical characteristics of different bin ary mixtures of solids on the spouting regime of a pyramidal square-based spouted bed reactor is assessed. The applied methodology permits a more precise evaluation of the effects of the tested variables (diameter, density, sphericity) on the response variables (minimum air flows at which spouting begins and at which to maintain spouting con ditions). The associated pressure drops along the bed of particles a nd the height of the formed fountai n are analysed in each case. During the initial stages of fluidisation, binary mixtures containing different density ratios show dead zones. Segregation becomes more evident at large-size and high-density ratios. The lack of sphericity was found to be the main reason leading to blocking, channelling, and start-up problems when system failures occur. Nevertheless, the extent of segregation in all cases decreases with increasing the spouting velocity. In addition, a computational fluid dynamic model based on the discrete element method, previously validated for a single solid bed, is proposed as a tool to predict and evaluate potential segregation phenomena in binary mixtures. This model reproduced with high accuracy the encountered segregation phenomena. Its use may help define the technical limts inherent in the pyramidal spouted bed reactor. | Cristina Moliner Filippo Marchelli Massimo Curti Barbara Bosio Giorgio Rovero Elisabetta Arato | 2019 | Particuology2019，17，2： | 4 |
| 17 | Unique microRNA molecular profiles in lung cancer diagnosis and prognosis显示文摘 | Nozomu Yanaihara Natasha Caplen Elise Bowman Masahiro Seike Kensuke Kumamoto Ming Yi Robert M. Stephens Aikou Okamoto Jun Yokota Tadao Tanaka George Adrian Calin Chang-Gong Liu Carlo M. Croce Curtis C. Harris | 2006 | Cancer Cell2006，，3： | 4 |
| 18 | 新氟喹诺酮类药物的抗微生物疗法显示文摘当前氟喹诺酮类药物不仅已成为发达国家如日本、美国、英国、德国、法国等许多国家的常用抗感染药物,而且在第三世界也得到迅速发展,如在印度该类药物的平均年增长率高达25%~28%.该类药物的口服制剂已成为治疗常见感染的重要药物,同时最近已证实,开始采用静脉给药然后改为口服给药的疗法已成为治疗某些严重感染的有效、安全、经济的疗法,尤其对多重耐药菌引起的严重感染有重要价值。最近因严重毒副作用而取消了刚刚在美国上市的替马沙星,虽然对该类药物市场可能有一定影响,但据 FDA 统计,其它已上市多年的品种如诺氟沙星、氧氟沙星和环丙沙星均没有像替马沙星那样多的严重毒副作用。环丙沙星1991年的销售额已达9.04亿美元,较1990年增加19.7%,仅次于头孢克罗.但目前已上市的品种对革兰氏阳性菌特别是肺炎链球菌的活性差,对耐甲氧西林的金葡菌、厌氧菌等活性也不理想,因此开发新品种的工作一直在大量继续进行.本文对了解国外该类药物现状有一定参考价值. | Curtis D Holt 王金生 | 1993 | 国外医药(抗生素分册)1993，14，6： | 4 |
| 19 | Regulation of hepatic micro RNA expression by hepatocyte nuclear factor 4 alpha显示文摘AIM To uncover the role of hepatocyte nuclear factor 4 alpha(HNF4α) in regulating hepatic expression of micro RNAs.METHODS Microarray and real-time PCR were used to determine hepatic expression of micro RNAs in young-adult mice lacking Hnf4α expression in liver(Hnf4α-Liv KO). Integrative genomics viewer software was used to analyze the public chromatin immunoprecipitation-sequencing datasets for DNA-binding of HNF4α, RNA polymerase-Ⅱ, and histone modifications to loci of micro RNAs in mouse liver and human hepatoma cells. Dual-luciferase reporter assay was conducted to determine effects of HNF4α on the promoters of mouse and human micro RNAs as well as effects of micro RNAs on the untranslated regions(3'UTR) of two genes in human hepatoma cells. RESULTS Microarray data indicated that most micro RNAs remained unaltered by Hnf4α deficiency in Hnf4α-Liv KO mice. However, certain liver-predominant micro RNAs were down-regulated similarly in young-adult male and female Hnf4α-Liv KO mice. The down-regulation of mi R-101, mi R-192, mi R-193 a, mi R-194, mi R-215, mi R-802, and mi R-122 as well as induction of mi R-34 and mi R-29 in male Hnf4α-Liv KO mice were confirmed by real-timePCR. Analysis of public chromatin immunoprecipitationsequencing data indicates that HNF4α directly binds to the promoters of mi R-101, mi R-122, mi R-194-2/mi R-192 and mi R-193, which is associated with histone marks of active transcription. Luciferase reporter assay showed that HNF4α markedly activated the promoters of mouse and human mi R-101b/mi R-101-2 and the mi R-194/mi R-192 cluster. Additionally, mi R-192 and mi R-194 significantly decreased activities of luciferase reporters for the 3'UTR of histone H3F3 and chromodomain helicase DNA binding protein 1(CHD1), respectively, suggesting that mi R-192 and mi R-194 might be important in chromosome remodeling through directly targeting H3F3 and CHD1.CONCLUSION HNF4α is essential for hepatic basal expression of a group of liver-enriched micro RNAs, including mi R-101, mi R-192, mi R-193 a, mi R-194 and mi R-802, through which HNF4α may play a major role in the post-transcriptional regulation of gene expression and maintenance of the epigenome in liver. | Hong Lu Xiaohong Lei Jerry Liu Curtis Klaassen | 2017 | World Journal of Hepatology2017，9，4： | 3 |
| 20 | Systematic review of risk factors for fibrosis progression in non-alcoholic steatohepatitis显示文摘 | Curtis K. Argo Patrick G. Northup Abdullah M.S. Al-Osaimi Stephen H. Caldwell | 2009 | Journal of Hepatology2009，，2： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员