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| 1 | The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis显示文摘Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots,'Huang Qin,' are used for liver and lung complaints and as complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408.14-Mb genome has been assembled into nine pseudochromosomes with a super-N50 of 33.2 Mb. Comparison of this sequence with those of closely related species in the order Lamiales, sesamum indicum and Salvia splendens,revealed that a specialized metabolic pathway for the synthesis of 4'-deoxyflavone bioactives evolved in the genus Scu-tellaria. We found that the gene encoding a specific cinnamate coenzyme A ligase likely obtained its newfunc- tion following recent mutations, and that four genes encoding enzymes in the 4'-deoxyflavone pathway are present as tandem repeats in the genome of S. baicalensis. Further analyses revealed that gene duplications, segmental duplication, gene amplification, and point mutations coupled to gene neo- and subfunctionaliza-tions were involved in the evolution of 4'-deoxyflavone synthesis in the genus Scutellaria. Our study not only provides significant insight into the evolution of specific flavone biosynthetic pathways in the mint family, Lamiaceae, but also will facilitate the development of tools for enhancing bioactive productivity by metabolic engineering in microbes or by molecular breeding in plants. The reference genome of S. baicalensis is also useful for improving the genome assemblies for other members of the mint family and offers an important foundation for decoding the synthetic pathways of bioactive compounds in medicinal plants. | Qing Zhao Jun Yang Meng-Ying Cui Jie Liu Yumin Fang Mengxiao Yan Wenqing Qiu Huiwen Shang Zhicheng Xu Reheman Yidiresi Jing-Ke Weng Tomas Pluskal Marielle Vigouroux Burkhard Steuernagel Yukun Wei Lei Yang Yonghong Hu Xiao-Ya Chen Cathie Martin | 2019 | Molecular Plant2019，12，7： | 23 |
| 2 | Photodegradation of methyl orange by BiOI-sensitized TiO_2显示文摘BiOI-sensitized titanium dioxide (TiO2) photocatalysts were prepared by a deposition method at room temperature and characterized by X-ray diffraction, X-ray photoelectron spectroscopy, and Brunauer-Emmett-Teller surface area measurements. The photocatalytic activities of the catalysts were evaluated for the degradation of methyl orange (MO) solution under UV and visible light irradiation. The effects of catalyst amount, initial pH value, initial concentration of MO, as well as KI amount were investigated. The repeatability of photocatalytic activity of the 1.7% BiOI/TiO2 catalyst was also tested. It is shown that BiOI sensitization enhances the photocatalytic activities of TiO2. With BiOI content increasing, the photocatalytic activities of BiOI/TiO2 under UV and visible light irradiation first increase, reaching a maximum around BiOI content of 1.7%, and then decrease with further increasing BiOI content. The 1.7% BiOI/TiO2 catalyst obviously exhibits much higher visible light photocatalytic activity than P25, and its UV light photocatalytic activity is slightly higher than that of P25. Under the conditions of a catalytic dose of 1.5 g.L-1, initial pH of 3.0, initial MO concentration of 20 mg.L-1, UV power of 300 W, and air flow rate of 0.8 L.min-1, complete degradatio is achieved within 60 min. The repeatability of photocatalytic activity of the 1.7% BiOI/TiO2 catalyst is highly reliable. | Li Huiquan Cui Yumin Hong Wenshan Hua Lin Tao Dongliang | 2012 | Rare Metals2012，31，6： | 10 |
| 3 | Degradation of BPB in photocatalysis enhanced by photosensitizer显示文摘The treatment of wastewater containing bromphenol blue (BPB) by WO3/α-Fe2O3 was studied. Reaction mecha- nism of photocatalysis enhanced by photosensitizer was probed. The relationships between the composition of heterogene- ous photocatalyst, the starting concentration of BPB, amount of photocatalyst, pH, amount of H2O2, illumination time and the decoloring rate of BPB were discussed. The results show that the decoloring rate of BPB can reach 99.1% by using WO3/α-Fe2O3 as heterogeneous photocatalyst, with the composition of m(WO3):m(α-Fe2O3 )=3:1, the initial concentration of BPB = 15mg/L, the amount of catalyst = 0.300 g, pH= 6.3, the amount of H2O2 =0.2 mL, and the illumination time = 6 h. | CUI Yumin SUN Wenzhong | 2006 | Rare Metals2006，25，2： | 5 |
| 4 | Photocatalytic degradation of MO by complex nanometer particles WO_3/TiO_2显示文摘Complex nanometer particles WO3/TiO2 were prepared using a sol-gel process and characterized using XRD spectra. The photocatalytic activity of TiO2 can be increased by doping W6+ with TiO2 because the doped W6+ that entered into the crystal lattices of TiO2 led to the formation of defects in the crystal lattices of TiO2 and thereby improved the photo- catalytic activity of TiO2.When WO3 doped in TiO2 exceeded 3%, the excess W6+ did not enter into the crystal lattices of TiO2 but were uniformly dispersed in TiO2 or they covered the surface of TiO2, which reduced the effective illumination area of TiO2 and thereby lowered the photocatalytic activity of TiO2.The relationship among the composition of the catalyst, the amount of photocatalyst, the illumination time, and the decolorizing rate of methyl orange (MO) were discussed. The results show that the decolorizing rate of MO can reach 82.3% using WO3/TiO2 as the photocatalyst, with the composition of WO3/TiO2 =3:97, the mass of catalyst = 0.400 g, the initial concentration of MO = 20 mg/L, pH = 6.5, and the illumina- tion time = 7 h. | CUI Yumin | 2006 | Rare Metals2006，25，6： | 3 |
| 5 | SbMYB3 transcription factor promotes root-specific f lavone biosynthesis in Scutellaria baicalensis显示文摘Scutellaria baicalensis Georgi produces abundant root-specific f lavones(RSFs),which provide various benefits to human health.We have elucidated the complete biosynthetic pathways of baicalein and wogonin.However,the transcriptional regulation of f lavone biosynthesis in S.baicalensis remains unclear.We show that the SbMYB3 transcription factor functions as a transcriptional activator involved in the biosynthesis of RSFs in S.baicalensis.Yeast one-hybrid and transcriptional activation assays showed that SbMYB3 binds to the promoter of flavone synthase II-2(SbFNSII-2)and enhances its transcription.In S.baicalensis hairy roots,RNAi of SbMYB3 reduced the accumulation of baicalin and wogonoside,and SbMYB3 knockout decreased the biosynthesis of baicalein,baicalin,wogonin,and wogonoside,whereas SbMYB3 overexpression enhanced the contents of baicalein,baicalin,wogonin,and wogonoside.Transcript profiling by qRT–PCR demonstrated that SbMYB3 activates SbFNSII-2 expression directly,thus leading to more abundant accumulation of RSFs.This study provides a potential target for metabolic engineering of RSFs. | Yumin Fang Jie Liu Minmin Zheng Sanming Zhu Tianlin Pei Mengying Cui Lijing Chang Hanwen Xiao Jun Yang Cathie Martin Qing Zhao | 2023 | Horticulture Research2023，10，2： | 2 |
| 6 | Effect of Mo 2 C content on the structure and photocatalytic property of Mo 2 C/TiO 2 catalysts显示文摘 | Huiquan Li Wenshan Hong Yumin Cui Suhua Fan Liangjun Zhu | 2013 | Journal of Alloys and Compounds2013，，： | 1 |
| 7 | Fabrication of in situ AlN-TiN/Al inoculant and its refining efficiency and reinforcing effect on pure aluminum显示文摘 | Kui Wang Chunxiang Cui Qian Wang Yumin Qi Chao Wang | 2013 | Journal of Alloys and Compounds2013，，： | 1 |
| 8 | The effect of carbon content on the structure and photocatalytie activity of nano-Bi2WO6 powder 显示文摘 | Cui Yumin Li Huiquan Hong Wenshan | 2013 | Powder Technology2013，247，： | 1 |
| 9 | The effect of carbon content on the structure and photocatalytic activity of nano-Bi2WO6 powder 显示文摘 | Cui Yumin Li Huiquan Hong Wenshan | 2013 | Powder Technology2013，247，： | 1 |
| 10 | High photocatalytic perfor- mance of BiOI/Bi2WO6 toward toluene and Reactive Brilliant Red 显示文摘 | Li Huiquan Cui Yumin Hong Wenshan | 2013 | Applied Surface Science2013，264，6： | 1 |
| 11 | Tensile Behavior of SiC Fiber-Reinforcedγ-TiAl Composites Prepared by Suction Casting显示文摘The process of preparing SiC_(f)iber-reinforced y-TiAl composites by the conventional methods is difficult and complicated due to the high reactivity,high melting point and poor deformability of y-TiAl alloys.In this work,suction casting,a promising method for preparing SiC_(f)/TiAl composite,had been attempted.In the process,y-TiAl alloy melt was introduced rapidly into a mold within pre-arranged fibers that were coated with additional layer of titanium alloy.This simple method successfully prevented serious reactions between the alloy melt and the fibers which remained intact during the solidification process.The interfacial reaction layer was observed by optical microscopy,scanning electron microscopy(SEM).The interfacial reaction products were identified by transmission electron microscopy(TEM).Tensile tests of the matrix alloy and composites were performed at room temperature and 800℃.The results exhibited that the tensile strength of SiC_(f)/γ-TiAl composite was higher than that of the matrix alloy at both room temperature and 8000 C.At room temperature,tensile strength of SiC_(f)/γ-TiAl composite was increased by about 7%(50 MPa),whereas a double increase in tensile strength 14%(100 MPa)was obtained at 800℃.The titanium alloy coating on the fiber not only prevented the serious interfacial reaction between the y-TiAl alloy melt and the SiC_(f)iber,but also played a role in delaying the propagation of cracks in the matrix to the fiber at 800℃.The fracture mechanism of the composite was analyzed by fracture metallographic analysis. | Yingying Shen Qing Jia Xu Zhang Ronghua Liu Yumin Wang Yuyou Cui Rui Yang | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，7： | 1 |
| 12 | Delivery of an Rhs-family nuclease effector reveals direct penetration of the gram-positive cell envelope by a type VI secretion system in Acidovorax citrulli显示文摘The type VI secretion system(T6SS)is a double-tubular nanomachine widely found in gram-negative bacteria.Its spear-like Hcp tube is capable of penetrating a neighboring cell for cytosol-to-cytosol protein delivery.However,gram-positive bacteria have been considered impenetrable to such T6SS action.Here we report that the T6SS of a plant pathogen,Acidovorax citrulli(AC),could deliver an Rhsfamily nuclease effector RhsB to kill not only gram-negative but also gram-positive bacteria.Using bioinformatic,biochemical,and genetic assays,we systematically identified T6SS-secreted effectors and determined that RhsB is a crucial antibacterial effector.RhsB contains an N-terminal PAAR domain,a middle Rhs domain,and an unknown C-terminal domain.RhsB is subject to self-cleavage at both its N-and C-terminal domains and its secretion requires the upstream-encoded chaperone EagT2 and VgrG3.The toxic Cterminus of RhsB exhibits DNase activities and such toxicity is neutralized by either of the two downstream immunity proteins,RimB1 and RimB2.Deletion of rhsB significantly impairs the ability of killing Bacillus subtilis while ectopic expression of immunity proteins RimB1 or RimB2 confers protection.We demonstrate that the AC T6SS not only can effectively outcompete Escherichia coli and B.subtilis in planta but also is highly potent in killing other bacterial and fungal species.Collectively,these findings highlight the greatly expanded capabilities of T6SS in modulating microbiome compositions in complex environments. | Tong-Tong Pei Yumin Kan Zeng-Hang Wang Ming-Xuan Tang Hao Li Shuangquan Yan Yang Cui Hao-Yu Zheng Han Luo Xiaoye Liang Tao Dong | 2022 | mLife2022，1，1： | 1 |
| 13 | High photocatalytic performance of Bi OI/Bi2WO6toward toluene and Reactive Brilliant Red显示文摘 | Li Huiquan Cui Yumin Hong Wenshan | 2013 | Applied Surface Science2013，264，1： | 1 |
| 14 | Research progress on the development mechanism and exploratory protection of the scaling off on earthen sites in NW China显示文摘The scaling off is among the most pervasive and severe deterioration types.The scaling off developed on earthen sites in Northwest China has a special and complex binary structure composed of crusted and loose layers.Under the synergistic functions of external environmental elements such as rainfall,temperature,and wind,and rammed earth properties,this deterioration has gradually created the crust on the site’s surface,and has then evolved into exfoliation and finally fallen off,leading to the reciprocating progressive destruction of earthen sites.Therefore,determining the development mechanism of scaling off and implementing targeted protection measures for the scientific conservation of earthen sites has become essential.In this review study,the properties of the building material causing the binary structure and the coupling effects of water,temperature,salt,and wind on the development of scaling off were determined,and the quantitative evaluation system for this deterioration development was elaborated.After interpreting the reasons for the development of the scaling off,protective measures including chemical grouting,electro-osmosis grouting,and overlay applications were stated.This study also pointed out the research hotspots and shortage in the study process on the scaling off.The hotspots generally focused on the development mechanism of binary structure,quantitative assessment of the degree of development,and related scientific consolidation measures.The shortcomings around the above directions include less attention to the development characteristics and mechanism of scaling off on earthen sites under special environment types,optimization and applicability of quantitative evaluation models,and the development and feasibility of reinforcement slurries and methods.This study can be beneficial in providing theoretical support for the scientific conservation and engineering practice of the scaling off on the earthen sites. | DU YuMin DONG WenQiang CUI Kai CHEN WenWu YANG WeiLi | 2023 | Science China(Technological Sciences)2023，66，8： | 0 |
| 15 | Gap-free genome assembly and CYP450 gene family analysis reveal the biosynthesis of anthocyanins in Scutellaria baicalensis显示文摘Scutellaria baicalensis Georgi,a member of the Lamiaceae family,is a widely utilized medicinal plant.The flavones extracted from S.baicalensis contribute to numerous health benefits,including anti-inflammatory,antiviral,and anti-tumor activities.However,the incomplete genome assembly hinders biological studies on S.baicalensis.This study presents the first telomere-to-telomere(T2T)gap-free genome assembly of S.baicalensis through the integration of Pacbio HiFi,Nanopore ultra-long and Hi-C technologies.A total of 384.59 Mb of genome size with a contig N50 of 42.44 Mb was obtained,and all sequences were anchored into nine pseudochromosomes without any gap or mismatch.In addition,we analysed the major cyanidin-and delphinidin-based anthocyanins involved in the determination of blue-purple flower using a widely-targeted metabolome approach.Based on the genome-wide identification of Cytochrome P450(CYP450)gene family,three genes(SbFBH1,2,and 5)encoding flavonoid 3′-hydroxylases(F3′Hs)and one gene(SbFBH7)encoding flavonoid 3′5′-hydroxylase(F3′5′H)were found to hydroxylate the B-ring of flavonoids.Our studies enrich the genomic information available for the Lamiaceae family and provide a toolkit for discovering CYP450 genes involved in the flavonoid decoration. | Tianlin Pei Sanming Zhu Weizhi Liao Yumin Fang Jie Liu Yu Kong Mengxiao Yan Mengying Cui Qing Zhao | 2023 | Horticulture Research2023，10，12： | 0 |
| 16 | Interplay between the atomic structures and superconductivity of two-monolayer Pb films显示文摘Superconductors with reduced dimensionality have been widely explored for their exotic superconducting behaviors.Especially,at the two-dimensional limit,two-monolayer Pb films with two types of structures provide an ideal platform to unveil the underlying superconducting mechanism[Science 324,1314(2009)].Here,by combining scanning tunneling microscopy(STM)with the first-principle calculations,we successfully identify that these two types have different atomic lattice structures with varying stacking phases,which further enables us to calculate the phonon spectrum and electron phonon coupling strength of each type.The theoretical calculations are in good agreement with tunneling spectroscopy measurements of the superconducting transition temperatures(T_(c)),which established a correlation between atomic structures and superconductivity.Moreover,it was observed that the higher T_(c)of these two types also possess higher out-of-plane upper critical magnetic fields(Hc2).These findings will provide important new insights into two-dimensional superconductivity at the atomic level. | Kun Xie Ze Huang Pengju Li Yumin Xia Desheng Cai Yitong Gu Yuzhou Liu Fangliang Cai Runxiao Zhang Haohao Shi Ping Cui Shengyong Qin | 2024 | Science China(Physics,Mechanics & Astronomy)2024，67，1： | 0 |
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