|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | HER2 testing in gastric cancer: An update显示文摘Human epidermal growth factor receptor 2(HER2) overexpression is increasingly recognized as a frequent molecular abnormality in gastric and gastroesophageal cancer. With the recent introduction of HER2 molecular targeted therapy for patients with advanced gastric cancer, determination of HER2 status is crucial in order to select patients who may benefit from this treatment. This paper provides an update on our knowledge of HER2 in gastric and gastroesophageal cancer, including the prognostic relevance of HER2, the key differences between HER2 protein expression interpretation in breast and gastric cancer, the detection methods and the immunohistochemistry scoring system. | Lucas Faria Abrahao-Machado Cristovam Scapulatempo-Neto | 2016 | World Journal of Gastroenterology2016，22，19： | 19 |
| 2 | HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays显示文摘AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma. | Lucas Faria Abraho-Machado Alexandre Andrade dos Anjos Jácome Durval Renato Wohnrath José Sebastio dos Santos Estela Cristina Carneseca José Humberto Tavares Guerreiro Fregnani Cristovam Scapulatempo-Neto | 2013 | World Journal of Gastroenterology2013，19，38： | 12 |
| 3 | Serrated pathway in colorectal carcinogenesis显示文摘Serrated adenocarcinoma is a recently described subset of colorectal cancer(CRC),which account for about10%of all CRCs and follows an alternative pathway in which serrated polyps replace the traditional adenoma as the precursor lesion to CRC.Serrated polyps form a heterogeneous group of colorectal lesions that includes hyperplastic polyps(HPs),sessile serrated adenoma(SSA),traditional serrated adenoma(TSA)and mixed polyps.HPs are the most common serrated polyp followed by SSA and TSA.This distinct histogenesis is believed to have a major influence in prevention strategies,patient prognosis and therapeutic impact.Genetically,serrated polyps exhibited also a distinct pattern,with KRAS and BRAF having an important contribution to its development.Two other molecular changes that have been implicated in the serrated pathway include microsatellite instability and the CpG island methylator phenotype.In the present review we will address the current knowledge of serrated polyps,clinical pathological features and will update the most recent findings of its molecular pathways.The understanding of their biology and malignancy potential is imperative to implement a surveillance approach in order to prevent colorectal cancer development. | Letícia Yamane Cristovam Scapulatempo-Neto Rui Manuel Reis Denise Peixoto Guimares | 2014 | World Journal of Gastroenterology2014，20，10： | 8 |
| 4 | Prognostic value of epidermal growth factor receptors in gastric cancer: a survival analysis by Weibull model incorporating long-term survivors显示文摘 | Alexandre Andrade Anjos Jácome Durval R. Wohnrath Cristovam Scapulatempo Neto Estela C. Carneseca Sérgio V. Serrano Luciano Souza Viana Jo?o S. Nunes Edson Z. Martinez José Sebasti?o Santos | 2014 | Gastric Cancer2014，，1： | 2 |
| 5 | Effect of adjuvant chemoradiotherapy on overall survival of gastric cancer patients submitted to D2 lymphadenectomy显示文摘 | Alexandre A. A. Jácome Durval R. Wohnrath Cristovam Scapulatempo Neto José Humberto T. G. Fregnani Ana Luiza Quinto Ant?nio T. T. Oliveira Vinícius L. Vazquez Gilberto Fava Edson Z. Martinez José S. Santos | 2013 | Gastric Cancer2013，，2： | 1 |
| 6 | Comparison betweenamniotic membrane with and without epithelium as a support forhuman limbal epithelial cells cultured ex vivo显示文摘 | Covre JL Loureiro RR Cristovam PC | 2011 | Arq Bras Oftalmol2011，74，2： | 1 |
| 7 | Importance of 3T3 feeder layer to establish epithelial cultures from cell suspension obtained from corneo-scleral rims显示文摘 | Cristovam PC Gl(o)ria MA Melo GB | | 0，，： | 1 |
| 8 | Comparison between amniotic membrane with and without epithelium as a support for human limbal epithelial cells cultured ex vivo显示文摘 | Covre JL Loureiro RR Cristovam PC | 2011 | Arq Bras Oftalmol2011，74，2： | 1 |
| 9 | Co-expression of monocarboxylate transporter 1 (MCT1) and its chaperone (CD147) is associated with low survival in patients with gastrointestinal stromal tumors (GISTs)显示文摘 | Ant?nio Talvane Torres Oliveira Céline Pinheiro Adhemar Longatto-Filho Maria Jose Brito Olga Martinho Delcio Matos André Lopes Carvalho Vinícius Lima Vazquez Thiago Buosi Silva Cristovam Scapulatempo Sarhan Sydney Saad Rui Manuel Reis Fátima Baltazar | 2012 | Journal of Bioenergetics and Biomembranes2012，，1： | 1 |
| 10 | High glucose concentration stimulates intracellular rennin activity and angiotcnsin Ⅱ generation in rat mesangialcells显示文摘 | Vidotti D B Casarini D E Cristovam P C | 2004 | Am J Phy siol Renal Physiol2004，286，： | 1 |
| 11 | High glucose concentration stimulates intracellular renin activity and angiotensin II generation in rat mesangial cells显示文摘 | Vidotti DB Casarini DE Cristovam PC | 2004 | Am J Physiol Renal Physiol2004，286，： | 1 |
| 12 | High glucose concentration stimulates intracellular renin activity and angiotensin II generation in rat mesangial cells显示文摘 | Vidotti DB Casarini DE Cristovam PC | 2004 | Am J Physiol Renal Physiol2004，286，2： | 1 |
| 13 | Transplantation of Conjunctival Epithelial Cells Cultivated Ex Vivo in Patients With Total Limbal Stem Cell Deficiency显示文摘 | José Reinaldo S. Ricardo Priscila C. Cristovam Pedro A. N. Filho Charles C. Farias Aline L. de Araujo Renata R. Loureiro Joyce L. Covre Jeison N. de Barros Telma P. Barreiro Myrna S. dos Santos José A. P. Gomes | 2013 | Cornea2013，，3： | 1 |
| 14 | High glu-cose concentration stimulates intracellular renin activityand angiotensin II generation in rat mesangial cells 显示文摘 | VidottiD B’Casarini D E Cristovam P C | 2004 | Am J Physiol Ren Physiol2004，286，： | 1 |
| 15 | Co-expression of monocarboxylate transporter 1 (MCT1) and its chaperone (CD147) is associated with low survival in patients with gastrointestinal stromal tumors (GISTs)显示文摘 | Ant?nio Talvane Torres Oliveira Céline Pinheiro Adhemar Longatto-Filho Maria Jose Brito Olga Martinho Delcio Matos André Lopes Carvalho Vinícius Lima Vazquez Thiago Buosi Silva Cristovam Scapulatempo Sarhan Sydney Saad Rui Manuel Reis Fátima Baltazar | 2012 | Journal of Bioenergetics and Biomembranes2012，，1： | 1 |
| 16 | High glucose concentration stimulates intracellular rennin activity and angiotensin Ⅱ generation in rat mesangial cells显示文摘 | Vidotti DB Casarini DE Cristovam PC | 2004 | Am J Physiol Renal Physiol2004，286，6： | 1 |
| 17 | Transplantation of conjunctival epithelial cells cultivated ex vivo in patients with total limbal stem cell deficiency显示文摘 | Ricardo JR Cristovam PC Filho PA | 2013 | Cornea2013，32，3： | 1 |
| 18 | Transplantation of Conjunctival Epithelial Cells Cultivated Ex Vivo in Patients With Total Limbal Stem Cell Deficiency显示文摘 | José Reinaldo S. Ricardo Priscila C. Cristovam Pedro A. N. Filho Charles C. Farias Aline L. de Araujo Renata R. Loureiro Joyce L. Covre Jeison N. de Barros Telma P. Barreiro Myrna S. dos Santos José A. P. Gomes | 2013 | Cornea2013，，： | 1 |
| 19 | High glucose con-centration stimulates intracellular renin activity and angioten-sin II generation in rat mesangial cells显示文摘 | Vidotti DB Casarini DE Cristovam PC | 2004 | Am J Physiol RenalPhysiol2004，286，6： | 1 |
| 20 | Role of chymase in diabetic nephropathy 显示文摘 | Cristovam PC Carmona AK Amoni CP | 2012 | Exp Biol Med (Maywood)2012，237，8： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员