|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Experimental obstructive jaundice alters claudin-4 expression in intestinal mucosa: Effect of bombesin and neurotensin显示文摘瞄准:调查试验性的妨碍的黄疸和外长的 bombesin 和 neurotensin (NT )(BBS ) 的影响紧密的连接(TJ ) 的表示上的管理在老鼠的肠上皮的蛋白质 claudin-4。方法:四十只男 Wistar 老鼠随机被划分成五个组:我 = 控制, II = 假冒操作, III = 胆汁管结扎(BDL ) , IV = BDL+BBS (30 microg/kg 每 d ) , V = BDL+NT (300 microg/kg 每 d ) 。在 d 的实验的结束 10,内毒素在门和大动脉的血被测量。终端回肠的织物节组织学地被检验并且免疫组织化学地为在肠上皮的 claudin-4 表示的评估。结果:妨碍的黄疸导致了重要的门和大动脉的 endotoxemia 表明的肠的障碍失败。Claudin-4 表示显著地被增加在上面在 jaundiced 老鼠的第三根绒毛并且一起来它的侧面的分发的规定被注意。BBS 或 NT 的管理恢复了 claudin-4 表示到控制状态并且显著地减少了门和大动脉的 endotoxemia。结论:在肠上皮的试验性的妨碍的黄疸增加 claudin-4 表示,它可以是贡献粘膜障碍的混乱的一个关键因素。毁坏规章的肽 BBS, NT 能阻止这扇改变和还原剂门和全身的 endotoxemia。 | Stelios F Assimakopoulos Constantine E Vagianos Aristides S Charonis Ilias H Alexandris Iris Spiliopoulou Konstantinos C Thomopoulos Vassiliki N Nikolopoulou Chrisoula D Scopa | 2006 | World Journal of Gastroenterology2006，12，21： | 21 |
| 2 | Effect of bombesin and neurotensin on gut barrier function in partially hepatectomized rats显示文摘AIM: To investigate the effect of regulatory peptides bombesin (BBS) and neurotensin (NT) on intestinal barrier function in partially hepatectomized rats.METHODS: Ninety male Wistar rats were randomly divided into five groups: Ⅰ (n = 10): controls, Ⅱ (n =20): sham operated, Ⅲ (n = 20): partial hepatectomy 70% (PHx), Ⅳ (n = 20): PHx+BBS (30 μg/kg/d), Ⅴ (n= 20): PHx+NT (300 μg/kg/d). Groups Ⅳ and Ⅴ were treated for 8 days before PHx and 48 h post surgery.At the end of the experiment, on day 10, intestinal barrier function was assessed by measuring endotoxin concentrations in portal and aortic blood. Tissue sections of the terminal ileum were examined histologically and villus density, mucosal thickness, mitotic activity and apoptosis in crypts were assessed. In addition, ileal mucosa was analyzed for DNA and protein content and microbiological analysis was performed in cecal contents.To estimate intestinal oxidative stress, lipid peroxidation was determined on tissue homogenates from terminal ileum.RESULTS: BBS or NT administration significantly reduced portal and systemic endotoxemia observed 48 h after partial hepatectomy. In hepatectomized rats (group Ⅲ), a trend towards induction of mucosal atrophy was observed, demonstrated by the reduction of villus density, mucosal thickness, protein content and significant reduction of DNA, while these alterations were reversed by regulatory peptides administration.This trophic effect of BBS and NT was accompanied by induction of mitoses above control levels and a significant reduction of apoptosis in intestinal crypts.Intestinal lipid peroxidation was found significantly lower in PHx group and regulatory peptides exerted an antioxidant action, further decreasing this parameter of oxidative stress. The bacterial population of E. coli and aerobic Gram (+) cocci was increased in cecal content of hepatectomized rats, while this parameter was not affected by the administration of BBS or NT.CONCLUSION: Gut regulatory peptides BBS and NT improve intestinal barrier function and reduce endotoxemia in experimental partial hepatectomy. This effect is, at least in part, mediated by their trophic,antiapoptotic, mitogenic, and antioxidant effect on the intestinal epithelium. This observation might be of potential value in patients undergoing liver resection. | Stelios F Assimakopoulos Ilias H Alexandris Chrisoula D Scopa Panagiotis G Mylonas Konstantinos C Thomopoulos Christos D Georgiou Vassiliki N Nikolopoulou Constantine E Vagianos | 2005 | World Journal of Gastroenterology2005，11，43： | 8 |
| 3 | Stimulation of oval cell and hepatocyte proliferation by exogenous bombesin and neurotensin in partially hepatectomized rats显示文摘AIM:To investigate the effect of the neuropeptides bombesin(BBS)and neurotensin(NT)on oval cell proliferation in partially hepatectomized rats not pretreated with a known hepatocyte inhibitor.METHODS:Seventy male Wistar rats were randomly divided into five groups:Ⅰ=controls,Ⅱ=sham operated,Ⅲ=partial hepatectomy 70%(PHx),Ⅳ=PHx+ BBS(30μg/kg per day),Ⅴ=PHx+NT(300μg/kg per day).Forty eight hours after liver resection,portal en-dotoxin levels and hepatic glutathione redox state were determined.α-fetoprotein(AFP)mRNA(in situ hybridisation),cytokeratin-19 and Ki67 antigen expression (immunohistochemistry)and apoptosis(TUNEL)were evaluated on liver tissue samples.Cells with morphological features of oval cells that were cytokeratin-19 (+)and AFP mRNA(+)were scored in morphometric analysis and their proliferation was recorded.In addition,the proliferation and apoptotic rates of hepatocytes were determined.RESULTS:In the control and sham operated groups,oval cells were significantly less compared to groups Ⅲ,ⅣandⅤ(P<0.001).The neuropeptides BBS and NT significantly increased the proliferation of oval cells compared to groupⅢ(P<0.001).In addition,BBS and NT induced a significant increase of hepatocyte proliferation(P<0.001),whereas it decreased their apoptotic activity(P<0.001)compared to groupⅢ.BBS and NT significantly decreased portal endotoxemia (P<0.001)and increased the hepatic GSH:GSSG ratio (P<0.05 and P<0.001,respectively)compared to groupⅢ.CONCLUSION:BBS and NT stimulated oval cell proliferation in a model of liver regeneration,without use of concomitant suppression of hepatocyte proliferation as oval cell activation stimuli,and improved the hepatocyte regenerative response.This peptides-induced combined stimulation of oval cell and hepatocyte proliferation might serve as a possible treatment modality for several liver diseases. | Stelios F Assimakopoulos Athanassios C Tsamandas Ilias H Alexandris Christos Georgiou Constantine E Vagianos Chrisoula D Scopa | 2011 | World Journal of Gastrointestinal Pathophysiology2011，2，6： | 2 |
| 4 | Frictional characteristics of teflon-steel interfaces under dynamic conditions显示文摘 | Constantinou M C Caccese J Harris H G | 1987 | Earthquake Engineering and Structural Dynamics1987，15，6： | 1 |
| 5 | Influence of C-sources on the denitrification rate of a high-nitrate concentrated industrial wastewater显示文摘 | Constantin H Fick M | 1997 | Water Research1997，31，3： | 1 |
| 6 | Influence of C-sources on the denitrification rate of a high-nitrate concentrated industrial wastewater显示文摘 | Constantin H Flck M | 1997 | Water Res1997，31，3： | 1 |
| 7 | Age-associated leukoaraiosis and cortical cholinergic deafferentation显示文摘 | N I. Bohnen M L.T.M. Müller H Kuwabara G M. Constantine S A. Studenski | 2009 | Neurology2009，，16： | 1 |
| 8 | Mini-dystrophin expression down-regulates overactivation of G protein-mediated IP3 signaling pathway in dystrophin-deficient muscle cells 显示文摘 | Balghi H Sebille S Constantin B | 2006 | Gen Physiol2006，127，2： | 1 |
| 9 | HIV testing technologies after two decodes of evolution 显示文摘 | Constantine NT Zink H | 2005 | Indian J Med Res2005，121，： | 1 |
| 10 | The partial substrate de thiaacetyl ~ coenzyme a mimics all critical carbon acid reactions in the condensation half-reaction catalyzed by thermoplasma acidophi lum citrate synthase显示文摘 | Kurz L C Constantine C Z Jiang H | 2009 | Biochemistry2009，48，33： | 1 |
| 11 | Paclitaxel and concurrent radiation in uppergastrointestinal cancers显示文摘 | Constantinou M Tsai J Y Safran H | 2003 | Cancer Invest2003，21，6： | 1 |
| 12 | New generation three - dimensional echocardiography for left ventficular volumetric and functional measurements: comparsion with cardiac magnetic resonance 显示文摘 | NIKTIN N P CONSTANTIN C LOH P H | 2006 | Eur J Echocardiogr2006，7，10： | 1 |
| 13 | A shallow water equation on the circle 显示文摘 | Constantin A McKean H P | 1999 | Communications on Pure and Applied Mathematics1999，52，8： | 1 |
| 14 | Hepatofugal Flow in the Portal Venous System: Pathophysiology, Imaging Findings, and Diagnostic Pitfalls 显示文摘 | Ronald H Phillip B Constantine T | 2002 | RadioGraphics2002，22，1： | 1 |
| 15 | Dynamics and control of cavitation during high-intensity focused ultrasound applica- tion显示文摘 | THOMAS C R FARNY C H CONSTANTIN C | 2005 | Acoustical Society of America2005，6，3： | 1 |
| 16 | The uniqueness of solutions of perturbed backward stochastic differential equation 显示文摘 | Constantin G H | 2004 | J Math Anal Appl2004，300，1： | 1 |
| 17 | New generation 3-dimensional echocardiography for left ventricular volumetric and functional measurements: comparison with cardiac magnetic resonance显示文摘 | Nikitin N P Constantin C Loh P H | 2006 | Eur J Echocardiogr2006，7，5： | 1 |
| 18 | Lenalidomide for the treatment of relapsed and refractory multiple myeloma显示文摘 | Gullu Gorgun Jakubikova Jacob Laubach Groen Anderson Van De Donk H Lokhorst Raymakers Nijhof Paul Richardson Teru Hideshima Constantine Mitsiades | 2012 | Cancer Management and Research (default)2012，，： | 1 |
| 19 | Paclitaxel andconcurrent radiation in upper gastroin-testinal cancers显示文摘 | Constantinou M Tsai J Y Safran H | 2003 | Cancer Invest2003，21，6： | 1 |
| 20 | Serological diagnosis of HIV in- fection using oral fluid samples显示文摘 | Tamashiro H Constantine NT | 1994 | Bull World Health Organ1994，72，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员