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| 1 | Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis显示文摘AIM: To investigate the intestinal anti-inflammatory effect and mechanism of a probiotic Lactobacillus sakuvarius ssp.salivariusCECT5713 in the TNBS model of rat colitis.METHODS: Female Wistar rats (180-200 g) were used in this study. A group of rats were administered orally the probiotic L. salivarius ssp. salivarius (5×108 CFU suspended in 0.5 mL of skimmed milk) daily for 3 wk. Two additional groups were used for reference, a non-colitic and a control colitic without probiotic treatment, which received orally the vehicle used to administer the probiotic. Two weeks after starting the experiment, the rats were rendered colitic by intracolonic administration of 10 mg of TNBS dissolved in 0.25 mL of 500 mL/L ethanol. One week after colitis induction, all animals were killed and colonic damage was evaluated both histologically and biochemically. The biochemical studies performed in colonic homogenates include determination of myeloperoxidase (MPO) activity,glutathione (GSH) content, leukotriene B4 (LTB4) and tumor necrosis factor α (TNF-α) levels, as well as inducible nitric oxide synthase (iNOS) expression. In addition, the luminal contents obtained from colonic samples were used for microbiological studies, in order to determine Lactobacilli and Bifidobacteria counts.RESULTS: Treatment of colitic rats with L. salivarius ssp.salivarius resulted in amelioration of the inflammatory response in colitic rats, when compared with the corresponding control group without probiotic treatment. This antiinflammatory effect was evidenced macroscopically by a significant reduction in the extent of colonic necrosis and/or inflammation induced by the administration of TNBS/ethanol (2.3+0.4 cm vs3.4+0.3 cm in control group,P<0.01) and histologically by improvement of the colonic architecture associated with a reduction in the neutrophil infiltrate in comparison with non-treated colitic rats. The latter was confirmed biochemically by a significant reduction of colonic MPO activity (105.3+26.0 U/g vs180.6±21.9 U/g, P<0.05), a marker of neutrophil infiltration. The beneficial effect was associated with an increase of the colonic GSH content (1 252±42 nmol/g vs 1 087+51 nmol/g, P<0.05),which is depleted in colitic rats, as a consequence of the oxidative stress induced by the inflammatory process. In addition, the treatment of colitic rats with L. salivarius resulted in a significant reduction of colonic TNF-α levels (509.4±68.2 pg/g vs782.9±60.1 pglg, P<0.01) and in a lower colonic iNOS expression, when compared to TNBS control animals without probiotic administration. Finally,treated colitic rats showed higher counts of Lactobacilli species in colonic contents than control colitic rats, whereas no differences were observed in Bifidobacteria counts.CONCLUSION: Administration of the probiotic L. salivarius ssp. salivarius CECT5713 facilitates the recovery of the inflamed tissue in the TNBS model of rat colitis, an effect associated with amelioration of the production of some of the mediators involved in the inflammatory response in the intestine, such as cytokines, including TNF-α and NO. This beneficial effect could be ascribed to its effect on the altered immune response that occurs in this inflammatory condition. | Laura Peran Desiree Camuesco Monica Comalada Ana Nieto Angel Concha Maria Paz Diaz-Ropero Monica Olivares Jordi Xaus Antonio Zarzuelo Julio Galvez | 2005 | World Journal of Gastroenterology2005，11，33： | 17 |
| 2 | 旋风分离器压力损失的数学模型及在设计中的应用显示文摘从理论上分析了旋风分离器压力损失的成因及影响因素,建立了旋风分离器压力损失的数学模型,解决了旋风分离器设计过程中压力损失的计算问题,将模型应用于实际工业型旋风分离器的设计得到的实际压力损失与数学模型的计算结果吻合良好。数学模型对旋风分离器的设计有较大的参考价值。 | 吴克明 石碤 潘留明 F Concha | 2005 | 化工环保2005，25，2： | 6 |
| 3 | IBD5 polymorphisms in inflammatory bowel disease: Association with response to infliximab显示文摘AIM: Inflammatory bowel diseases (IBD) are multifactorial pathologies of unknown etiology. One susceptibility locus,IBD5, has been mapped to chromosome 5q31. We analyzed our Spanish cohorts of Crohn's disease (CD)and ulcerative colitis (UC) patients to determine whether this locus is associated with IBD, and to ascertain the main clinical phenotype influenced by this risk factor. The kind of interaction, either genetic heterogeneity or epistasis, between this IBD5 susceptibility region and the NOD2/CARD15 gene mutations was studied as well.Finally, ve assessed whether this locus can predict response to infliximab therapy.METHODS: A case control study was performed with 274CD and 211 UC patients recruited from a single center and 511 healthy ethnically matched controls. Two polymorphisms were genotyped in the IBD5 locus and three in the CARD15/NOD2 gene.RESULTS: Our results evidence association only with CD especially with the fistulizing phenotype and in the absence of NOD2/CARD15 variants (mutant allele frequency in patients vscontrols: OR = 2.03, 95% CI = 1.35-3.06,P<0.01). The frequency of the IBD5 homozygous mutant genotype significantly increased in CD patients lacking response to infliximab (RR = 3.88, 95% CI = 1.18-12.0,P<0.05). UC patients overall do not show association with 5q31 polymorphisms, although a similar trend to the one observed in CD is found within the worse prognosis group.CONCLUSION: The IBD5 variants may enhance an individual carrier's risk for CD, mainly in the absence of the NOD2/CARD15 mutations and in fistulizing patients.The data presented suggest the potential role of the 5q31polymorphisms as markers of response to infiiximab. | Elena Urcelay Juan Luis Mendoza Alfonso Martínez Laura Fernández Carlos Taxonera Manuel Díaz-Rubio Emilio G.de la Concha | 2005 | World Journal of Gastroenterology2005，11，8： | 4 |
| 4 | Parameter Control of Genetic Algorithms by Learning and Simulation of Bayesian Networks——A Case Study for the Optimal Ordering of Tables显示文摘Parameter setting for evolutionary algorithms is still an important issue in evolutionary computation. There are two main approaches to parameter setting: parameter tuning and parameter control. In this paper, we introduce self-adaptive parameter control of a genetic algorithm based on Bayesian network learning and simulation. The nodes of this Bayesian network are genetic algorithm parameters to be controlled. Its structure captures probabilistic conditional (in)dependence relationships between the parameters. They are learned from the best individuals, i.e., the best configurations of the genetic algorithm. Individuals are evaluated by running the genetic algorithm for the respective parameter configuration. Since all these runs are time-consuming tasks, each genetic algorithm uses a small-sized population and is stopped before convergence. In this way promising individuals should not be lost. Experiments with an optimal search problem for simultaneous row and column orderings yield the same optima as state-of-the-art methods but with a sharp reduction in computational time. Moreover, our approach can cope with as yet unsolved high-dimensional problems. | Concha Bielza Juan A. Fernndez del Pozo Pedro Larranaga | 2013 | Journal of Computer Science & Technology2013，28，4： | 2 |
| 5 | Embolization of Bronchial Arteries of Anomalous Origin显示文摘 | Concha Sancho Elena Escalante Joan Domínguez José Vidal Eva Lopez Joan Valldeperas Xavier J. Monta?á | 1996 | CardioVascular and Interventional Radiology1996，，4： | 2 |
| 6 | Genetic polymorphisms influencing arsenic metabolism: evidence from Argentina显示文摘 | Schlawicke EKS Broberg K Concha G ct al | 2007 | Environ Health Perspcct2007，115，： | 1 |
| 7 | Different patterns of HLA - DR antigen expression in normal epithelium ,hyperplastic and neoplastic malignant lesions of the breast显示文摘 | Concha A Ruiz- Cabello F Cabrera T | 1995 | Eur J Immunogenet11995，22，4： | 1 |
| 8 | On the construction of global weak solutions in the kynch theory of sedimentation显示文摘 | BUTOS M C CONCHA F | 1988 | Math Methods in the Appl Sci1988，10，： | 1 |
| 9 | Flow cytometry as a method for studying effects of stressors on primary rat neurons显示文摘 | Behbahani H Rickle A Concha H | 2005 | J Neurosci Res2005，82，： | 1 |
| 10 | White-matter diffusion abnormalities in temporal-lobe epilepsy with and without mesial temporal sclerosis显示文摘 | Concha L Beaulieu C Collins DL | 2009 | J Neurol Neurosurg Psychiatry2009，80，： | 1 |
| 11 | Enzymatic pretreat- ment on rose - hip oil extraction: hydrolysis and pressing conditions显示文摘 | CONCHA J SOTO C CHAMY R | 2004 | J Am Oil Chem Soc2004，81，6： | 1 |
| 12 | Se- vere persistent off in young goats显示文摘 | de la CONCHA - BERMEJILLO A GUO J ZHANG Z | 2003 | J Vet Diagn Invest2003，15，5： | 1 |
| 13 | Distinct white matter abnormali- ties in different idiopathic generalized epilepsy syndromes 显示文摘 | Liu M Concha L Beaulieu C | 2011 | Epilepsia2011，52，： | 1 |
| 14 | Procalcitonin and C-reactive protein as markers of systemic inflammatory response syndrome severity in critically ill children 显示文摘 | Corsino Rey Marta Los Arcos Andr e s Concha | 2007 | Intensive Care Med2007，33，3： | 1 |
| 15 | Comparison of procalcition with C reaction protein and serum amyloid for the early diagnosis of bacterial sepsis in critically ill neonates and children显示文摘 | Enguix A Rey C Concha A | 2001 | Int Care Med2001，27，1： | 1 |
| 16 | Develop-ment of an environmental friendly method for the analysis of or-ganochlorine pesticides in sediments显示文摘 | Concha G E Fernandez G V Grueiro N G et a1 | 2010 | Chemosphere2010，79，7： | 1 |
| 17 | Environmental arsenic exposure and DNA methylation of the tumor suppressor gene pl6 and the DNA repair gene MLH1 : effect of arsenic metabolism and genotype 显示文摘 | Hossain M B Vahter M Concha G | 2012 | Metallomics2012，4，11： | 1 |
| 18 | Genetic polylnorphisms influencing arsenic metabolism: evidence from Argentina 显示文摘 | Engstrom KS Broberg K Concha G | 2007 | Environ Health Perspect2007，115，： | 1 |
| 19 | Comparison of procalcition with C reaction protein and serum amyloid for the early diagnosis of bacterial sepsis in critically ill neonates and children 显示文摘 | Enguix A Rey C Concha A | 2001 | Int Care Med2001，27，1： | 1 |
| 20 | Agrobacterium-mediated transient transformation of marigold( Tagetes erec- ta ) 显示文摘 | Godoy-Hernandez G Berzunza E A Concha L C | 2006 | Plant Cell Tissue and Organ Culture2006，84，3： | 1 |
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