|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa显示文摘Background: Lycopene(LYC) is a natural carotenoid with powerful reactive oxygen species(ROS) scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate(Fe AA). Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment(0.25, 0.5, 1 or 2 mmol/L) in the presence or absence of Fe AA(150 μmol/L Fe SO_4 and 750 μmol/L ascorbic acid) during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision?computer-aided sperm analysis(CASA) system. Cell viability was examined with the metabolic activity(MTT) assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium(NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) as well as the concentrations of glutathione(GSH) and malondialdehyde(MDA).Results: FeA A treatment led to a reduced spermatozoa motility(P < 0.001), viability(P < 0.001) and a decline of the antioxidant capacity of spermatozoa(P < 0.001) but increased the ROS generation(P < 0.001), superoxide production(P < 0.001) and lipid peroxidation(P < 0.001). LYC administration resulted in a preservation of the spermatozoa motion parameters(P < 0.001), mitochondrial activity(P < 0.001) and antioxidant characteristics(P < 0.001 with respect to SOD;P < 0.01 in relation to CAT; P < 0.05 as for GPx and GSH) with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions: Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells. | Eva Tvrdá Anton Kovácik Eva Tusimová Dusan Paál Alica Mackovich Jakhongir Alimov Norbert Lukác | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 3 |
| 2 | Development of novel coatable compatibilized polyimide-modified silica nanocomposites显示文摘 | Toheed Akhter Humaira Masood Siddiqi Shaukat Saeed O. Ok Park Sung Cik Mun | 2014 | Journal of Polymer Research2014，，5： | 1 |
| 3 | Carbon and nitrate utilization in soils: The effect of long-term fertilization on potential denitrification显示文摘 | Simek M Kal cik J | 1998 | Geoderma1998，83，34： | 1 |
| 4 | 显示文摘 | KVASNI CKA F SEV CIK R VOLD RICH M | 2006 | J Chromatogr: A2006，1113，： | 1 |
| 5 | Cadmium and nickel uptake are differentially modulated by salicylic acid in Matricaria chamomilla plants显示文摘 | KOV(A)CIK J GRZ J HEDBAVNY J | 2009 | J Agric Food Chem2009，57，： | 1 |
| 6 | Effect of copper and salicylic acid on phenolic metabolites and free amino acids in Scenedesmus quadricauda (Chlorophyceae) 显示文摘 | KovCt Cik J Klejdus B Hedbavny J | 2010 | Plant Science2010，178，3： | 1 |
| 7 | Molecular characteriza- tion of N-methyl-D-aspartate receptors expressed in mamma- lian cells yields evidence for the coexistence of three suhunit types within a discrete receptor molecule显示文摘 | Chazot PL Coleman SK Cik M | 1994 | J Biol Chem1994，269，24： | 1 |
| 8 | Stepwise posttranslational processing of progrowth hormone releasing hormone (proGHRH) polypeptide by furin and PC1 显示文摘 | Posner SF Vaslet CA Jurof cik M | 2004 | Endocrine2004，23，23： | 1 |
| 9 | 显示文摘 | Ryszkowska J L Augus'cik M Sheikh A | 2010 | Composites Science and Technology2010，70，： | 1 |
| 10 | On spaces Lp(x)and Wk,p(x)显示文摘 | Kovácik O Rákosník J | 1991 | Czechoslovak Math J1991，41，4： | 1 |
| 11 | The effects of neurosteroids on picrotoxin-,bicuculline- and NMDA-induced seizures,and a hypnotic effect of ethanol显示文摘 | Czonkowska AI Krza??cik P Sienkiewicz-Jarosz H Siemiatkowski M Szyndler J Bidziński A Pa??nik A | 2000 | Pharmacol Biochem Behav2000，67，2： | 1 |
| 12 | Intermodal interfer-ence in a photonic crystal fibre显示文摘 | Kácik D Turek I Martincek I | 2004 | Optics Express2004，12，15： | 1 |
| 13 | Use o f Gas Chromatogra phy for Determnatio n of Components of Reaction Mixture in Synthesis of Acrylic acid 显示文摘 | Linkiewicz Mar ia Szo cik Alkin | 1991 | Chemical Analysis (Warsaw)1991，36，56： | 1 |
| 14 | Developing socioeconomic indicators for fisheries off Alaska: A multi-attribute utility function approach 显示文摘 | Seung C Zhang CIK | 2011 | Fisheries Research2011，112，3： | 1 |
| 15 | Single channel properties of cloned NMDA receptors in a human cell line: comparison with results from Xenopus oocytes显示文摘 | STERN P CIK M COLQUHOUN D | 1994 | J Physiol1994，476，3： | 1 |
| 16 | Skin permeation of acyl derivatives of stobadine 显示文摘 | M~jekov~i M Koprda V Boh~cik L | 2006 | Drug Deliv2006，13，1： | 1 |
| 17 | On Spaces L^p(x) and W^l,p(x) 显示文摘 | KOV'AˇCIK O R'AKOSNIK J | 1991 | Czechoslovak Math1991，41，： | 1 |
| 18 | Effect of amylose content on structure,texture andα-amylase reactivity of cooked rice显示文摘 | LU S CIK T T LII C Y | 2013 | LWT-food science and technology2013，54，： | 1 |
| 19 | Daphnane-type diterpene orthoesters and their biological activities 显示文摘 | He W D Cik M Appendino G | 2002 | Mini-Rev Med Chem2002，2，2： | 1 |
| 20 | The effect ofsilicon on maize growth under cadmium stress显示文摘 | Dresler S Woj cik M Bednarek W | 2015 | Russ J PlantPhysl2015，，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员