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| 1 | COR27 and COR28 encode nighttime repressors integrating Arabidopsis circadian clock and cold response显示文摘It was noted that circadian components function in plant adaptation to diurnal temperature cycles and freezing tolerance.Our genome-wide transcriptome analysis revealed that evening-phased COR27 and COR28 mainly repress the transcription of clockassociated evening genes PRR5,ELF4 and cold-responsive genes.Chromatin immunoprecipitation indicated that CCA1 is recruited to the site containing EE elements of COR27 and COR28 promoters in a temperaturedependent way.Further genetic analysis shows COR28 is essential for the circadian function of PRR9 and PRR7.Together,our results support a role of COR27 and COR28 as nighttime repressors integrating circadian clock and plant cold stress responses. | peng wang xuan cui chunsheng zhao liyan shi guowei zhang fenglong sun xiaofeng cao li yuan qiguang xie xiaodong xu | 2017 | Journal of Integrative Plant Biology2017，59，2： | 6 |
| 2 | Macrophage membrane-coated nanocarriers Co-Modified by RVG29 and TPP improve brain neuronal mitochondria-targeting and therapeutic efficacy in Alzheimer's disease mice显示文摘Neuronal mitochondrial dysfunction caused by excessive reactive oxygen species(ROS)is an early event of sporadic Alzheimer's disease(AD),and considered to be a key pathologic factor in the progression of AD.The targeted delivery of the antioxidants to mitochondria of injured neurons in brain is a promising therapeutic strategy for AD.A safe and effective drug delivery system(DDS)which is able to cross the blood-brain barrier(BBB)and target neuronal mitochondria is necessary.Recently,bioactive materials-based DDS has been widely investigated for the treatment of AD.Herein,we developed macrophage(MA)membrane-coated solid lipid nanoparticles(SLNs)by attaching rabies virus glycoprotein(RVG29)and triphenylphosphine cation(TPP)molecules to the surface of MA membrane(RVG/TPP-MASLNs)for functional antioxidant delivery to neuronal mitochondria.According to the results,MA membranes camouflaged the SLNs from being eliminated by RESrich organs by inheriting the immunological characteristics of macrophages.The unique properties of the DDS after decoration with RVG29 on the surface was demonstrated by the ability to cross the BBB and the selective targeting to neurons.After entering the neurons in CNS,TPP further lead the DDS to mitochondria driven by electric charge.The Genistein(GS)-encapsulated DDS(RVG/TPP-MASLNs-GS)exhibited the most favorable effects on reliveing AD symptoms in vitro and in vivo by the synergies gained from the combination of MA membranes,RVG29 and TPP.These results demonstrated a promising therapeutic candidate for delaying the progression of AD via neuronal mitochondria-targeted delivery by the designed biomimetic nanosystems. | Yang Han Chunhong Gao Hao Wang Jiejie Sun Meng Liang Ye Feng Qianqian Liu Shiyao Fu Lin Cui Chunsheng Gao Yi Li Yang Yang Baoshan Sun | 2021 | Bioactive Materials2021，6，2： | 4 |
| 3 | Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma显示文摘Nanosuspensions,as a new drug delivery system for insoluble drugs,are only composed of a drug and a small amount of stabilizer,which is dispersed in an aqueous solution with high drug-loading,small particle size,high dispersion,and large specific surface area.It can significantly improve the dissolution,bioavailability,and efficacy of insoluble drugs.In this study,paclitaxel nanosuspensions((PTX)NS)were prepared by an ultrasonic precipitation method,with the characteristics of simple preparation and easy repetition.With the help of a homologous targeting mechanism,a kind of glioma C6 cancer cell membrane(CCM)-coated(PTX)NS was developed and modified with DWSW peptide to obtain DWSW-CCM-(PTX)NS with the functions of BBB penetration and tumor targeting.The results showed that the cancer cell membrane could effectively camouflage the nanosuspensions so that it was not cleared by the immune system and could cross the blood-brain-barrier(BBB)and selectively target tumor tissues.Cell uptake experiments and in vivo imaging confirmed that the uptake of DWSW-CCM-(PTX)NS by tumor cells and the distribution in intracranial gliomas increased.Cytotoxicity test and in vivo anti-glioma studies showed that DWSW-CCM-(PTX)NS could significantly inhibit the growth of glioma cells and significantly prolong the survival time of glioma-bearing mice.Finally,the cancer cell membrane coating endowed the nanosuspensions with the biological properties of homologous adhesion and immune escape.This study provides an integrated solution for improving the targeting of nanosuspensions and demonstrates the encouraging potential of biomimetic nanosuspensions applicable to tumor therapy. | Yueyue Fan Yuexin Cui Wenyan Hao Mengyu Chen Qianqian Liu Yuli Wang Meiyan Yang Zhiping Li Wei Gong Shiyong Song Yang Yang Chunsheng Gao | 2021 | Bioactive Materials2021，6，12： | 3 |
| 4 | Prediction of Deformation and Footprint of High-speed Rotating Radial Tires显示文摘In this research,the tire deformations due to rotation are divided into three parts:tread,belt and carcass.Tread deformation is obtained by using a rotating hollow cylinder model,belt expansion is calculated according to the laminated composite shell theory,and a formula of dynamic equilibrium profile is built to investigate carcass deformation.Moreover,a simple method is used to predict the footprint changes caused by tire deformation.Results show that a large deformation is occurring at tire shoulder,a smaller one at tread centre,and the shoulder deformations are mostly caused by carcass.Length of footprint side edges increase with tire rolling speed increasing.The predicted tread,belt,carcass deformations and footprint changes are in good agreement with finite element analysis results.The proposed method and the results will offer a helpful guidance to tire and vehicle engineers. | CUI Zhibo CHAI Delong LYU Jiafeng HU Kai ZHANG Chunsheng HOU Dandan WANG Youshan | 2020 | 橡胶工业2020，67，5： | 2 |
| 5 | On-farm evaluation of an in-season nitrogen management strategy based on soil N min test显示文摘 | Zhenling Cui Fusuo Zhang Xinping Chen Yuxin Miao Junliang Li Liwei Shi Jiufei Xu Youliang Ye Chunsheng Liu Zhiping Yang Qiang zhang Shaomin Huang Dejun Bao | 2007 | Field Crops Research2007，，1： | 1 |
| 6 | Application of recombinant enzymes in the production of human insulin 显示文摘 | Chunsheng Leng Hang Cui | 2011 | Journal of Chinese Pharmaceutical Science2011，23，5： | 1 |
| 7 | On-farm evaluation of an in-season nitrogen management strategy based on soil N min test显示文摘 | Zhenling Cui Fusuo Zhang Xinping Chen Yuxin Miao Junliang Li Liwei Shi Jiufei Xu Youliang Ye Chunsheng Liu Zhiping Yang Qiang zhang Shaomin Huang Dejun Bao | 2007 | Field Crops Research2007，，1： | 1 |
| 8 | Suppression of matrix metalloproteinase-9 expression by RNA interference inhibits SGC7901 gastric adenocarcinoma cell growth and invasion in vitro and in vivo显示文摘 | Fengjuan Zhao Qingyu Zhang Chunsheng Kang Xiaowei Cui Tao Wang Peng Xu Xuan Zhou Jian Liu Xiaomei Song | 2010 | Medical Oncology2010，，3： | 1 |
| 9 | On-farm estimation of indigenous nitrogen supply for site-specific nitrogen management in the North China plain显示文摘 | Zhenling Cui Fusuo Zhang Xinping Chen Yuxin Miao Junliang Li Liwei Shi Jiufei Xu Youliang Ye Chunsheng Liu Zhiping Yang Qiang Zhang Shaomin Huang Dejun Bao | 2008 | Nutrient Cycling in Agroecosystems2008，，1： | 1 |
| 10 | The Research of Temperature Compensa- tion Technology of High-Temperature Pressure Sensor 显示文摘 | Ma Tiehua Cui Chunsheng | 2011 | Interna- tional Conference on Electronic2011，11，： | 1 |
| 11 | Research on Recommendation Algorithms Based on Cloud Models in Probabilistic Linguistic Environments显示文摘To solve the problem that the traditional cloud model can't directly process the textual review information in the recommendation algorithm,this paper combines the merits of the cloud model in transforming qualitative and quantitative knowledge with the multi-granularity advantages of probabilistic linguistic term sets in representing uncertain information,and proposes a recommendation algorithm based on cloud model in probabilistic language environment.Initially,this paper quantifies the attributes in the review text based on the probabilistic linguistic term set.Subsequently,the maximum deviation method is used to determine the weight of each attribute in the evaluation information of the product to be recommended,and the comprehensive evaluation number and attribute weight are converted into the digital characteristic value of the cloud model by using the backward cloud generator.Finally,the products are recommended and sorted based on the digital characteristic value of the cloud model.The algorithm is applied to the recommendation of 10 hotels,and the results show that the method is effective and practical,enriching the application of cloud models in the recommendation field. | Peng YANG Xifeng MA Meng WEI Chunsheng CUI Libin CHE | 2024 | Journal of Systems Science and Information2024，12，1： | 0 |
| 12 | Expert opinion on translational research for advanced glioblastoma treatment显示文摘Malignant gliomas are known to be one of the most difficult diseases to diagnose and treat because of the infiltrative growth pattern,rapid progression,and poor prognosis.Many antitumor drugs are not ideal for the treatment of gliomas due to the blood-brain barrier.Temozolomide(TMZ)is a DNA alkylating agent that can cross the blood-brain barrier.As the only first-line chemotherapeutic drug for malignant gliomas at present,TMZ is widely utilized to provide a survival benefit;however,some patients are inherently insensitive to TMZ.In addition,patients could develop acquired resistance during TMZ treatment,which limits antitumor efficacy.To clarify the mechanism underlying TMZ resistance,numerous studies have provided multilevel solutions,such as improving the effective concentration of TMZ in tumors and developing novel small molecule drugs.This review discusses the in-depth mechanisms underlying TMZ drug resistance,thus aiming to provide possibilities for the establishment of personalized therapeutic strategies against malignant gliomas and the accelerated development and transformation of new targeted drugs. | Xiaoteng Cui Yunfei Wang Junhu Zhou Qixue Wang Chunsheng Kang | 2023 | Cancer Biology & Medicine2023，20，5： | 0 |
| 13 | Identification of the E2F1-RAD51AP1 axis as a key factor in MGMT-methylated GBM TMZ resistance显示文摘Objective:Epidermal growth factor receptor variant III(EGFRvIII)is a constitutively-activated mutation of EGFR that contributes to the malignant progression of glioblastoma multiforme(GBM).Temozolomide(TMZ)is a standard chemotherapeutic for GBM,but TMZ treatment benefits are compromised by chemoresistance.This study aimed to elucidate the crucial mechanisms leading to EGFRvIII and TMZ resistance.Methods:CRISPR-Cas13a single-cell RNA-seq was performed to thoroughly mine EGFRvIII function in GBM.Western blot,realtime PCR,flow cytometry,and immunofluorescence were used to determine the chemoresistance role of E2F1 and RAD51-associated protein 1(RAD51AP1).Results:Bioinformatic analysis identified E2F1 as the key transcription factor in EGFRvIII-positive living cells.Bulk RNA-seq analysis revealed that E2F1 is a crucial transcription factor under TMZ treatment.Western blot suggested enhanced expression of E2F1 in EGFRvIII-positive and TMZ-treated glioma cells.Knockdown of E2F1 increased sensitivity to TMZ.Venn diagram profiling showed that RAD51AP1 is positively correlated with E2F1,mediates TMZ resistance,and has a potential E2F1 binding site on the promoter.Knockdown of RAD51AP1 enhanced the sensitivity of TMZ;however,overexpression of RAD51AP1 was not sufficient to cause chemotherapy resistance in glioma cells.Furthermore,RAD51AP1 did not impact TMZ sensitivity in GBM cells with high O6-methylguanine-DNA methyltransferase(MGMT)expression.The level of RAD51AP1 expression correlated with the survival rate in MGMT-methylated,but not MGMT-unmethylated TMZ-treated GBM patients.Conclusions:Our results suggest that E2F1 is a key transcription factor in EGFRvIII-positive glioma cells and quickly responds to TMZ treatment.RAD51AP1 was shown to be upregulated by E2F1 for DNA double strand break repair.Targeting RAD51AP1 could facilitate achieving an ideal therapeutic effect in MGMT-methylated GBM cells. | Junhu Zhou Fei Tong Jixing Zhao Xiaoteng Cui Yunfei Wang Guangxiu Wang Chunsheng Kang Xiaomin Liu Qixue Wang | 2023 | Cancer Biology & Medicine2023，20，5： | 0 |
| 14 | Corrigendum to“Macrophage membrane-coated nanocarriers Co-Modified by RVG29 and TPP improve brain neuronal mitochondria-targeting and therapeutic efficacy in Alzheimer’s disease mice”[Bioactive materials 6(2021)529-542]显示文摘The authors regret publishing the incorrect address and affiliation.They have been now revised as follows:1.The Addresses are updated from.“a School of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang,110016,PR China b State Key Laboratory of Toxicology and Medical Countermeasures,Beijing Institute of Pharmacology and Toxicology,Beijing,100850,PR China c School of Functional Food and Wine,Shenyang Pharmaceutical University,Shenyang,10016,PR China. | Yang Han Chunhong Gao Hao Wang Jiejie Sun Meng Liang Ye Feng Qianqian Liu Shiyao Fu Lin Cui Chunsheng Gao Yi Li Yang Yang Baoshan Sun | 2022 | Bioactive Materials2022，7，1： | 0 |
| 15 | Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma显示文摘Background Metabolism reprogramming plays a vital role in glioblastoma(GBM)progression and recurrence by producing enough energy for highly proliferating tumor cells.In addition,metabolic reprogramming is crucial for tumor growth and immune-escape mechanisms.Epidermal growth factor receptor(EGFR)amplification and EGFR-vIII mutation are often detected in GBM cells,contributing to the malignant behavior.This study aimed to investigate the functional role of the EGFR pathway on fatty acid metabolism remodeling and energy generation.Methods Clinical GBM specimens were selected for single-cell RNA sequencing and untargeted metabolomics analysis.A metabolism-associated RTK-fatty acid-gene signature was constructed and verified.MK-2206 and MK-803 were utilized to block the RTK pathway and mevalonate pathway induced abnormal metabolism.Energy metabolism in GBM with activated EGFR pathway was monitored.The antitumor effect of Osimertinib and Atorvastatin assisted by temozolomide(TMZ)was analyzed by an intracranial tumor model in vivo.Results GBM with high EGFR expression had characteristics of lipid remodeling and maintaining high cholesterol levels,supported by the single-cell RNA sequencing and metabolomics of clinical GBM samples.Inhibition of the EGFR/AKT and mevalonate pathways could remodel energy metabolism by repressing the tricarboxylic acid cycle and modulating ATP production.Mechanistically,the EGFR/AKT pathway upregulated the expressions of acyl-CoA synthetase short-chain family member 3(ACSS3),acyl-CoA synthetase long-chain family member 3(ACSL3),and long-chain fatty acid elongation-related gene ELOVL fatty acid elongase 2(ELOVL2)in an NF-κB-dependent manner.Moreover,inhibition of the mevalonate pathway reduced the EGFR level on the cell membranes,thereby affecting the signal transduction of the EGFR/AKT pathway.Therefore,targeting the EGFR/AKT and mevalonate pathways enhanced the antitumor effect of TMZ in GBM cells and animal models.Conclusions Our findings not only uncovered the mechanism of metabolic reprogramming in EGFR-activated GBM but also provided a combinatorial therapeutic strategy for clinical GBM management. | Xiaoteng Cui Jixing Zhao Guanzhang Li Chao Yang Shixue Yang Qi Zhan Junhu Zhou Yunfei Wang Menglin Xiao Biao Hong Kaikai Yi Fei Tong Yanli Tan Hu Wang Qixue Wang Tao Jiang Chuan Fang Chunsheng Kang | 2023 | Cancer Communications2023，43，12： | 0 |
| 16 | Group Decision-Making Method of Entry Policy During a Pandemic显示文摘Omicron,the new mutant coronavirus,has spread rapidly globally,attracting close attention from different stakeholders worldwide.The complex and constantly changing epidemic situation has had a new impact on the world.Therefore,this paper focuses on the characteristics of the rapid spread of the COVID-19 variant strain.Generally,epidemic prevention experts conduct preliminary screening as part of the existing epidemic plan database according to the current local situation,after which they sort the alternatives deemed more suitable for the situation.Then the decision-makers identify the most divergent expert group,plan for consultation and adjustments,and finally obtain the plan with the smallest divergence.This article aims to integrate the experts'opinions with the method of minimizing the differences,which can maximize the expert consensus and help organize the schemes that best meet the epidemic situation.The experts'negotiation and iteration of the differences in the initial plan align with the current complex and dynamic epidemic situation and are of great significance to the rapid formulation of plans to achieve effective prevention and control. | Chunsheng Cui Baiqiu Li Xianfeng Chen | 2024 | Tsinghua Science and Technology2024，29，1： | 0 |
| 17 | Simultaneous transcatheter intervention for atrial septal defect complicated with patent ductus arteriosus: A 13-year single institutional retrospective study显示文摘Purpose: The feasibility and validity of simultaneous transcatheter interventions for patients with atrial septal defect(ASD) complicated with patent ductus arteriosus(PDA) has not been systematically evaluated. Materials and Methods: A retrospective analysis was conducted in patients who received transcatheter procedures for ASD complicated with PDA concurrently. The indications and treatment protocols were in accordance with the current guidelines. The sequence of therapy for ASD complicated with PDA was determined by clinical experience. Patients were followed up for at least 6 months after therapy Results: Overall, 22 patients received simultaneous transcatheter interventional therapy, and the success rate was a 100%. No severe complications transpired during the procedure or follow-up stage. Conclusion: Simultaneous transcatheter intervention is feasible and effective for patients who have concurrent complications for both ASD and PDA. | Qiguang Wang Xianyang Zhu Zhongchao Wang Chunsheng Cui Duanzhen Zhang Chuanju Hou Po Zhang Huoyuan Chen Lili Meng | 2018 | Journal of Interventional Medicine2018，1，1： | 0 |
| 18 | Analysis and Practice of "Idea, Team, and Evaluation" in the Educational Reform of Higher Vocational Colleges显示文摘 | Hongwei YI Lingling CUI Chunsheng SUN | 2013 | International Journal of Technology Management2013，，10： | 0 |
| 19 | New Equations for Describing Contact Area between Tire and Ground显示文摘New equations are proposed to describe the shapes and sizes of the contact areas between tire and ground(the contact areas)under the conditions of static load,slip,camber,and rolling.The equations are super ellipse and trigonometric function with four parameters.Results indicate that the contact areas under the conditions of static load,slip,camber can be well described by super ellipse,in the most complicated case,fourteen parameters are used.The rolling contact area can be reconstructed by the trigonometric function completely.Symmetrical and asymmetrical shapes can both be described perfectly.The suggested curves will make calculate size of the areas easily.The described shapes and sizes are in good agreement with the measured,the maximum deviation is only 2.88%.This work will provide an instrument for understanding the contact area and be helpful for analyzing tire and road. | CUI Zhibo SU Zhaoqian HOU Dandan ZHANG Chunsheng WANG Youshan LIU Yuyan WU Jian | 2020 | 橡胶工业2020，67，9： | 0 |
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