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| 1 | Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules显示文摘由病毒的 transduction 的四抄写因素 Oct4, Klf4, Sox2 和 c-Myc 的介绍能导致体的房间的 reprogramming 进导致的 pluripotent 干细胞(iPSCs ) ,但是 iPSCs 的使用被病毒的交货系统的使用妨碍。导致化学药品的 reprogramming 把一条新奇途径提供给没有任何病毒的基于向量的基因修正,产生 iPSCs。尽管,以前的报告证明几个小分子能代替一些 reprogramming 因素至少二个抄写因素, Oct4 和 Klf4,仍然被要求从老鼠产生 iPSCs 胚胎的成纤维细胞。这里,我们识别特定的化学联合,它是足够的面对一个单个抄写因素从胚胎的老鼠和成年成纤维细胞允许 reprogramming, Oct4 在 20 天以内,代替 Sox2, Klf4 和 c-Myc。用这个处理产生的 iPSCs 类似于胚胎的干细胞以全球基因表示介绍的老鼠,在 vitro 并且在 vivo 的 epigenetic 地位和 pluripotency。我们也发现那 8 天 Oct4 正式就职是足够的面对小分子启用导致 Oct4 的 reprogramming,它建议 reprogramming 在开始的 8 天以内被开始并且独立于连续外长的 Oct4 表示。没有基因修正,这些发现将帮助 iPSCs 的未来产生,以及阐明位于 reprogramming 过程下面的分子的机制。 | Yanqin Li Qiang Zhang Xiaolei Yin Weifeng Yang Yuanyuan Du Pingping Hou Jian Ge Chun Liu Weiqi Zhang Xu Zhang Yetao Wu Honggang Li Kang Liu Chen Wu Zhihua Song Yang Zhao Yan Shi Hongkui Deng | 2011 | Cell Research2011，21，1： | 51 |
| 2 | Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems显示文摘The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM(available at http://gffzz103f3ce5d9b14419hcuk596uk09uq69wo.ffgz.tsg.suse.edu.cn/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing(NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers,cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems. | Qing Liu Chun Wang Xiaozhen Jiao Huawei Zhang Lili Song Yanxin Li Caixia Gao Kejian Wang | 2019 | Science China(Life Sciences)2019，62，1： | 37 |
| 3 | Short-term entecavir therapy of chronic severe hepatitis B显示文摘BACKGROUND:Chronic severe hepatitis B patients often have limited survival.This investigation aimed to evaluate the short-term effects of nucleoside analog therapy on chronic severe hepatitis B. METHODS:We retrospectively,randomly collected the data of 129 chronic severe hepatitis B patients:55 were treated with entecavir,and the remaining 74 were not treated with nucleoside analogues. RESULTS:No significant difference in short-term survival rate was found between the group treated with entecavir and that treated without nucleoside analogues.Although entecavir greatly reduced HBV replication in different periods of therapy(P<0.001),the model for end-stage liver disease(MELD)score and liver function(alanine aminotransferase,albumin,bilirubin,prothrombin time) showed no significant change.No significant differences were found in MELD scores and liver function in patients with different HBV DNA levels(≤104copies/ml,>10 4 to<10 6 copies/ml,≥10 6 copies/ml).Nor correlation was observed between HBV DNA levels and MELD scores in different periods of therapy(P>0.05).The HBV DNA levels of patients who survived for over 3 months or less than 3 months were not significantly different either.However, the MELD score and parameters of liver function(albumin, bilirubin,prothrombin time)were different between the two groups(P<0.05).CONCLUSION:These results suggest that short-term suppression of HBV replication may not slow down the progression of liver failure in patients with chronic severe hepatitis B. | Chen, Jun Han, Jian-Hua Liu, Chun Yu, Ren-He Li, Fa-Zhao Li, Qun-Fang Gong, Guo-Zhong | 2009 | Hepatobiliary & Pancreatic Diseases International2009，8，3： | 31 |
| 4 | Expression and significance of proapoptotic gene Bax in gastric carcinoma显示文摘INTRODUCTIONRecentinvestigationshavedemonstratedthatapoptosisplaysasignificantroleinthepathogenesisoftumors[1,2].Emphasishas... | LIU Hai Feng, LIU Wei Wen, FANG Dian Chun and MEN Rong Pu | 1999 | World Journal of Gastroenterology1999，5，1： | 32 |
| 5 | Clinical efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole for community-acquired pneumonia with aspiration factors显示文摘 | Sun Tieying Sun Li Wang Rongmei Ren Xiaoping Sui Dong-jiang Pu Chun Ren Yajuan Liu Ying Yang Zhuo Li Fengzhi | 2014 | Chinese Medical Journal2014，，7： | 34 |
| 6 | Expanding the Range of CRISPR/Cas9 Genome Editing in Rice显示文摘 | Xixun Hu Chun Wang Yaping FU Qing Liu Xiaozhen Jiao Kejian Wang | 2016 | Molecular Plant2016，9，6： | 31 |
| 7 | A semi-spring and semi-edge combined contact model in CDEM and its application to analysis of Jiweishan landslide显示文摘Continuum-based discrete element method(CDEM)is an explicit numerical method used for simulation of progressive failure of geological body.To improve the efficiency of contact detection and simplify the calculation steps for contact forces,semi-spring and semi-edge are introduced in calculation.Semispring is derived from block vertex,and formed by indenting the block vertex into each face(24semisprings for a hexahedral element).The formation process of semi-edge is the same as that of semi-spring(24semi-edges for a hexahedral element).Based on the semi-springs and semi-edges,a new type of combined contact model is presented.According to this model,six contact types could be reduced to two,i.e.the semi-spring target face contact and semi-edge target edge contact.By the combined model,the contact force could be calculated directly(the information of contact type is not necessary),and the failure judgment could be executed in a straightforward way(each semi-spring and semi-edge own their characteristic areas).The algorithm has been successfully programmed in C++program.Some simple numerical cases are presented to show the validity and accuracy of this model.Finally,the failure mode,sliding distance and critical friction angle of Jiweishan landslide are studied with the combined model. | Chun Feng Shihai Li Xiaoyu Liu Yanan Zhang | 2014 | Journal of Rock Mechanics and Geotechnical Engineering2014，6，1： | 34 |
| 8 | The Association of Maternal Body Composition and Dietary Intake with the Risk of Gestational Diabetes Mellitus during the Second Trimester in a Cohort of Chinese Pregnant Women显示文摘Objective To investigate the association of maternal body composition and dietary intake with the risk of gestational diabetes mellitus(GDM). Methods A total 154 GDM subjects and 981 controls were enrolled in a prospective cohort study in 11 hospitals from May 20, 2012 to December 31, 2013. Bioelectrical impedance analysis and dietary surveys were used to determine body composition and to evaluate the intake of nutrients in subjects at 21-24 weeks' gestation(WG). Logistic regression analysis was applied to explore the relationships of maternal body composition and dietary intake with the risk of GDM morbidity. Results Age, pre-pregnant body weight(BW), and body mass index(BMI) were associated with increased risk of GDM. Fat mass(FM), fat mass percentage(FMP), extracellular water(ECW), BMI, BW, energy, protein, fat, and carbohydrates at 21-24 WG were associated with an increased risk of GDM. In contrast, fat free mass(FFM), muscular mass(MM), and intracellular water(ICW) were associated with a decreased risk of GDM. Conclusion Maternal body composition and dietary intake during the second trimester of pregnancy were associated with the risk of GDM morbidity. | Xu Qing Gao Zhi Ying Li Li Ming Wang Lu Zhang Qian Teng Yue Zhao Xia Ge Sheng Jing Hong Jiang Yang Yong Tao Liu Xiao Jun Lyu Chun Jian Mao Lun Yu Xiao Ming Liu Ying Hua Kong Ai Jing Yang Xue Yan Liu Zhao Zhang Yong Wang Jin Zhang Xin Sheng Xue Chang Yong Lu Yan Ping | 2016 | Biomedical and Environmental Sciences2016，29，1： | 29 |
| 9 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 10 | Indoor Allergen Levels and Household Distributions in Nine Cities Across China显示文摘Objective Chinese allergic subjects have high levels of sensitization to house dust mite(HDM) and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f 1 from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA. Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from <0.006 to 9.2 μg/g of dust, depending on the city. The percentages of households having HDM allergen levels associated with the risk of developing allergy sensitization and asthma were 65% and 25%, respectively. Specific antigens of the storage mite and cockroach were only found in samples from the southern and tropical regions of China. Levels of mite allergens were generally higher in samples from bedding compared to samples from the living room, even for storage mites, whereas levels of cockroach antigens were higher in the living room samples. Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens. | ZHENG Yi Wu LAI Xu Xin ZHAO De Yu ZHANG Chun Qing CHEN Jian Jun ZHANG Luo WEI Qing Yu CHEN Shi LIU En Mei NORBACK Dan GJESING Birgitte ZHONG Nan Shan SPANGFORT D.Michael | 2015 | Biomedical and Environmental Sciences2015，28，10： | 24 |
| 11 | Expression of bcl-2 protein in gastric carcinoma and its significance显示文摘Expressionofbcl2proteiningastriccarcinomaanditssignificanceLIUHaiFeng,LIUWeiWen,FANGDianChunandMENRongPuSubjectheadings... | LIU Hai Feng, LIU Wei Wen, FANG Dian Chun and MEN Rong Pu | 1998 | World Journal of Gastroenterology1998，4，3： | 23 |
| 12 | Development and application of biological technologies in fish genetic breeding显示文摘Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties.For the purpose of genetic improvement,researchers can select for desirable genetic traits,integrate a suite of traits from different donors,or alter the innate genetic traits of a species.These improved varieties have,in many cases,facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield.In this review,we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies(containing traditional selective breeding,molecular marker-assisted breeding,genome-wide selective breeding and breeding by controlling single-sex groups),integration breeding technologies(containing cross breeding,nuclear transplantation,germline stem cells and germ cells transplantation,artificial gynogenesis,artificial androgenesis and polyploid breeding)and modification breeding technologies(represented by transgenic breeding)in fish genetic breeding.Additionally,we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish,including distant hybridization,gynogenesis,and androgenesis.Finally,we systematically summarize the research status and known problems associated with each technology. | XU Kang DUAN Wei XIAO Jun TAO Min ZHANG Chun LIU Yun LIU ShaoJun | 2015 | Science China(Life Sciences)2015，58，2： | 23 |
| 13 | Acute Respiratory Distress Syndrome: Challenge for Diagnosis and Therapy显示文摘 | Chun Pan Ling Liu Jian-Feng Xie Hai-Bo Qiu | 2018 | Chinese Medical Journal2018，，10： | 22 |
| 14 | Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp显示文摘This study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (G3), which was generated from the diploid eggs produced by the second gynogenetic generation (G2) of red crucian carp × common carp, and determined the chromosomal numbers of G3, G2×scatter scale carp and G2×allotetraploid hybrids of red crucian carp × common carp. The results showed that the diploid eggs of G2 with 100 chromosomes, activated by UV-irradiated sperm from scatter scale carp and without the treatment for doubling the chromosomes, could develop into G3 with 100 chromosomes. Similar to the first and second gynogenetic generations (G1 and G2), G3 was also diploid (2n=100) and presented the hybrid traits. The triploids (3n=150) and tetraploids (4n=200) were produced by crossing G2 with scatter scale carp and crossing G2 with allotetraploids, respectively. The extrusion of the second polar body in the eggs of G2 ruled out the possibility that the retention of the second polar body led to the formation of the diploid eggs. In addition, we discussed the mechanism of the formation of the diploid eggs generated by G2. The establishment of the diploid gynogenesis clonal line (G1, G2 and G3) provided the evidence that the diploid eggs were able to develop into a new diploid hybrid clonal line by gynogenesis. By producing the diploid eggs as a unique reproductive way, the diploid gyno- genetic progeny of allotetraploid hybrids of red crucian carp × common carp had important signifi- cances in both biological evolution and production application. | LIU ShaoJun, DUAN Wei, TAO Min, ZHANG Chun, SUN YuanDong, SHEN JiaMin, WANG Jing, LUO KaiKun & LIU Yun Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2007 | Science China(Life Sciences)2007，50，2： | 21 |
| 15 | The efficacy and safety of weekly 35-mg risedronate dosing regimen for Chinese postmenopausal women with osteoporosis or osteopenia: 1-year data显示文摘目的: 当每周,每天管理了时,口头的 risedronate 在绝经后的骨质疏松症的处理是有效的。在这 1 年,使随机化,双窗帘,我们比较了的 multicenter 学习在有骨质疏松症或 osteopenia 的中国绝经后的女人的处理的每周的 35-mg 和每日的 5-mg risedronate dosing 政体。 | Jie-mei GU Li WANG Hua LIN De-cai CHEN Hai TANG Xiao-lan JIN Wei-bo XIA Yun-qiu HU Wen-zhen FU Jin-wei HE Hao ZHANG Chun WANG Hua YUE Wei-wei HU Yu-juan LIU Zhen-lin ZHANG | 2015 | Acta Pharmacologica Sinica2015，36，7： | 19 |
| 16 | Progress in Nanocellulose Preparation and Application显示文摘Nanocellulose is a biodegradable, renewable, nonmeltable polymeric material that is insoluble in most solvents due to hydrogen bonding and crystallinity. Nanocellulose has attracted considerable attention in recent decades owing to its environmental friendliness, wide availability, good biocompatibility, high crystallinity, and high Young's modulus. This review presents the recent achievements in preparation and applications of nanocellulose, including a discussion of the advantages and disadvantages of various preparation methods and a summary of the applications of nanocellulose in composite materials research. Finally, we examine the mounting evidence of more widespread potential applications of nanocellulose. | HaiQuan Mao YongYang Gong YuanLi Liu ShiQi Wang LinLin Du Chun Wei | 2017 | Paper And Biomaterials2017，2，4： | 19 |
| 17 | QTL editing confers opposing yield performance in different rice varieties显示文摘Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci(QTLs). In the past decade, many yield-contributing QTLs have been identified in crops.However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas_9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds. | Lan Shen Chun Wang Yaping Fu Junjie Wang Qing Liu Xiaoming Zhang Changjie Yan Qian Qian Kejian Wang | 2018 | Journal of Integrative Plant Biology2018，60，2： | 18 |
| 18 | Angiopoietin-1 protects mesenchymal stem cells against serum depriva-tion and hypoxia-induced apoptosis through the PI3K/Akt pathway显示文摘瞄准:表明系统的 angiopoietin-1 (Angl )/Tie-2 不仅在容器生长起一个枢轴的作用,改变,并且 endothelial,神经原,和 cardiomyocytes 的成熟,而且还原剂 apoptosis。然而,相对,很少至于 Angl 是否在间充质的干细胞(MSC ) 上有保护的效果被知道。现在的学习的目的是调查对浆液剥夺和导致组织缺氧的 apoptosis 在 MSC 上发信号的 Angl/Tie-2 的保护的效果,并且决定可能的机制。方法:Hoechst 33342 和终端 deoxynucleotidyl 标记染色的调停 transferase 的 digoxigenin-dUTP 刻痕结束被用来估计 MSC 的 apoptosis。Tie-2, Akt, Bcl-2, Bax,和劈开的 caspase-9 和 -3 的表示被西方的污点分析检测。结果:这研究证明 MSC 表示了 Tie-2 受体,并且 Angl 导致了 Tie-2 受体磷酸化。MSC 上的 Angl 的保护的效果是剂量依赖者并且在 50 ug/L 达到顶点;然而,可溶的 Tie-2/Fc 熔化蛋白质,它由扣押充当一个禁止者 Angl,废除了 anti-apoptotic 效果。Angl 导致了 Akt 磷酸化,增加了 Bcl-2/Bax 比率,并且减少 caspase-9 和 -3 的激活。所有这些效果被 Tie-2/Fc 和 phosphatidylinositol 稀释 3 激酶(PI3K ) 禁止者, wortmannin。结论:这些结果证明 Angl 能保护 MSC 免于浆液剥夺和导致组织缺氧的 apoptosis;Angl/Tie-2 发信号和它的下游的 PI3K/Akt 送信人小径在导致 MSC 幸存的过程是关键的。 | Xian-bao LIU Jun JIANG Chun GUI Xin-yang HU Mei-xiang XIANG Jian-an WANG | 2008 | Acta Pharmacologica Sinica2008，29，7： | 18 |
| 19 | Biostratigraphy of Triassic Marine Reptiles in Southwest Guizhou and Its Adjacent Area显示文摘This paper briefly reviews the Triassic marine reptile fossils inGuizhou Province, especially the fossils that have been recently found in the Guanling area. Based on three sections at Guanling and Xingyi, Guizhou Province and Luoping, Yunnan Province, four horizons with vertebrate fossils are recognized in the Middle and Upper Triassic of this area; They are from bottom to top: Member I and Member Ⅱ of the Guanling Formation, and the Zhuganpo Member and the Wayao Member of the Falang Formation. | WANG Liting, LI Jinling, WANG Xinjin, LI Chun,WU Tianzhuang and LIU Jun Bureau of Geology and Mineral Exploration and Development of Guizhou ProvinceGuiyang 550004 Institute of Vertebrate Palaeontology and Palaeoanthropology,Chinese Academy of Sciences, Beijing 100044 Guizhou Museum, Guiyang 550004 | 2001 | Acta Geologica Sinica(English Edition)2001，75，4： | 17 |
| 20 | Targeted mutagenesis in rice using CRISPR-Cpf1 system显示文摘Cpfl is a class 2/type V CRISPR effector that has been recently harnessed for genome editing(Zetsche et al.,2015;Hur et al.,2016;Kim et al.,2016).Cpfl recognizes thymidine-rich sequence as the protospacer-adjacent motif(PAM)at the 5'end of target sequences.In addition,Cpfl requires only a single shorter crRNA | Xixun Hu Chun Wang Qing Liu Yaping Fu Kejian Wang | 2017 | Journal of Genetics and Genomics2017，44，1： | 17 |
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