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| 1 | TRPV1 and TRPA1 in cutaneous neurogenic and chronic inflammation: pro-inflammatory response induced by their activation and their sensitization显示文摘皮肤的神经原的发炎(CNI ) 是被导致的发炎(或提高) 在由从感觉神经末端的 neuropeptides 的版本的皮肤。临床的表明主要是象瘙痒和红斑那样的感觉、脉管的混乱。短暂受体潜力 vanilloid 1 并且 ankyrin 1 (TRPV1 和 TRPA1,分别地) 是知道明确地参予疼痛和 CNI 的非选择的阳离子隧道。TRPV1 和 TRPA1 在感觉神经的一个大子集被共同表示,在他们集成众多的有害刺激的地方。两条隧道的表示也在皮肤在感觉神经以外远延长,现在是清楚的,在 keratinocytes,桅杆房间,树枝状的房间,和 endothelial 房间也发生。在这些 non-neuronal 房间, TRPV1 和 TRPA1 也充当 nociceptive 传感器并且加强煽动性的过程。这评论在在感觉神经原和 non-neuronal 皮肤房间导致或维持 CNI 的煽动性的基因的调整讨论 TRPV1 和 TRPA1 的角色。另外,这评论由支持 CNI 的自我维护的另外的内长的煽动性的调停人提供关于两条 TRP 隧道的细胞内部的促进感受性小径的当前的研究的一篇摘要。 | Olivier Gouin Killian L'Herondelle Nicolas Lebonvallet Christelle Le Gall-lanotto Mehdi Sakka Virginie Buhe Emmanuelle PIee-Gautier Jean-Luc Carre Luc Lefeuvre Laurent Misery~,Raphaele Le Garrec | 2017 | Protein & Cell2017，8，9： | 45 |
| 2 | Bactericidal and anti-adhesive properties of culinary and medicinal plants against Helicobacter pylori显示文摘AIM: To investigate the bactericidal and anti-adhesive properties of 25 plants against Helicobacter pylori (H pylori).METHODS: Twenty-five plants were boiled in water to produce aqueous extracts that simulate the effect of cooking. The bactericidal activity of the extracts was assessed by a standard kill-curve with seven strains of H pylori. The anti-adhesive property was assessed by the inhibition of binding of four strains of FITC-labeled H pylori to stomach sections. RESULTS: Of all the plants tested, eight plants, including Bengal quince, nightshade, garlic, dill, black pepper, coriander, fenugreek and black tea, were found to have no bactericidal effect on any of the isolates. Columbo weed, long pepper, parsley, tarragon, nutmeg, yellow-berried nightshade, threadstem carpetweed, sage and cinnamon had bactericidal activities against H pylori, but total inhibition of growth was not achieved in this study. Among the plants that killed H pylori, turmeric was the most efficient, followed by cumin, ginger, chilli, borage, black caraway, oregano and liquorice. Moreover, extracts of turmeric; borage and parsley were able to inhibit the adhesion of H pylori strains to the stomach sections.CONCLUSION: Several plants that were tested in our study had bactericidal and/or anti-adhesive effects on H pylori. Ingestion of the plants with anti-adhesive properties could therefore provide a potent alternative therapy for H pylori infection, which overcomes the problem of resistance associated with current antibiotic treatment. | Rachel O'Mahony Huda Al-Khtheeri Deepaka Weerasekera Neluka Fernando Dino Vaira John Holton Christelle Basset | 2005 | World Journal of Gastroenterology2005，11，47： | 29 |
| 3 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 4 | 源于近端股骨间充质干细胞免疫抑制作用的研究显示文摘目的探讨源于成人近端股骨的间充质干细胞(MSC)是否具有免疫抑制功能,为临床移植物抗宿主病的防治寻找理想的细胞储备库。方法从全髋关节置换术患者的近端股骨中分离并培养MSC,将不同数量的细胞直接或以Transwell法加入混合淋巴细胞反应(MLR)中,检测MSC对外周血淋巴细胞(PBL)增殖水平的影响。以ELISA法及抗体中和法探讨细胞因子TGFβ1在MSC免疫调节中的作用。结果3×103、1×104、3×104、1×105个源于近端股骨的MSC对PBL增殖的抑制率分别为62%±18%、38%±4%、31%±16%、28%±12%,与采自髂骨骨髓穿刺物MSC的抑制作用相比差异无统计学意义(均P>0.05),后者对PBL的增殖抑制率为58%±12%、37%±8%、28%±6%、27%±6%;以0.2μm的微孔膜对MSC与MLR进行分隔,3×104及1×104个MSC对PBL的增殖抑制率为36%±8%和53%±12%;在ELISA实验中,MSC单独培养,MSC与MLR直接接触,或经Transwell共培养的这3种上清中转化生长因子(TGF)β1的表达水平差异无统计学意义,加入浓度递增的TGFβ1抗体不能逆转MSC对PBL增殖的抑制作用。结论源于近端股骨的MSC具有一定的免疫抑制功能,除TGFβ1外其他可溶性因子可能介导这一作用。 | 张翼鷟 Loic Fouillard Alain Chapel Morad Bensidhoum Christelle Mazurier Aish Nasef Sandrine Bouchet Manuel Lopez Dominique Thierry Norbert Cclaucle Gorin 达万明 | 2005 | 中华医学杂志2005，85，39： | 11 |
| 5 | Effects of resolvin D1 on inflammatory responses and oxidative stress of lipopolysaccharide-induced acute lung injury in mice显示文摘 | Yuan Ruixia Wang Lei Yao Chengyue Wu Qingping Marie Christelle Xie Wanli Zhang Xingcai Sun Wei Wang Huiqing Yao Shanglong | 2014 | Chinese Medical Journal2014，，5： | 10 |
| 6 | Dual Resistance of Melon to Fusarium oxysporum Races 0 and 2 and to Papaya ring-spot virus is Controlled by a Pair of Head-to-Head-Oriented NB-LRR Genes of Unusual Architecture显示文摘Dear Editor, Potyviruses such as Papaya ring-spot virus (PRSV) cause important yield losses in cucurbits.Two distinct resistant alleles were identified in the Cucumis melo germplasm.Accession PI 414723 (Supplemental Table 1) possesses monogenic resistance,controlled by the Prv2 allele,and reacts to PRSV by systemic necrotic lesions;plants with the Prv1 allele,described in cultivar WMR-29,remain symptomless (Pitrat and Lecoq,1983).Fusarium oxysporum f.sp.melonis (FUS)exclusively attacks melon,causing severe wilt.Monogenic dominant resistance was described against races 0,1,and 2.The Fom-2 gene,controlling resistance to races 0 and 1,was cloned by Joobeur et al.(2004),and encodes a nucleotide binding domain (NB)-leucine rich repeat (LRR) protein.Our study focused on the Fom-1 gene,which confers resistance to races 0 and 2 (Risser et al.,1976),and on the Prv gene;the two are tightly linked on melon linkage group iX.Molecular markers were developed for the Fom-1/Prv locus,but no study has provided the resolution required for positional cloning. | Yariv Brotman Michael Normantovich Zachi Goldenberg Zvi Zvirin Irina Kovalski Nastacia Stovbun Tirza Doniger Anthony M. Bolqer Christelle Troadec Abdelhafid Bendahman~ RoniCohen Nurit Katzir Michel Pitrat Catherine Dogimont Rafael PerI-Treves | 2013 | Molecular Plant2013，6，1： | 9 |
| 7 | Oxidation state specific analysis of arsenic species in tissues of wild-type and arsenic(+3 oxidation state)methyltransferase-knockout mice显示文摘Arsenic methyltransferase(As3mt) catalyzes the conversion of inorganic arsenic(i As) to its methylated metabolites, including toxic methylarsonite(MAs~Ⅲ) and dimethylarsinite(DMAs~Ⅲ). Knockout(KO) of As3 mt was shown to reduce the capacity to methylate i As in mice. However, no data are available on the oxidation states of As species in tissues of these mice. Here, we compare the oxidation states of As species in tissues of male C57BL/6 As3mt-KO and wild-type(WT) mice exposed to arsenite(iA s~Ⅲ) in drinking water. WT mice were exposed to50 mg/L As and As3mt-KO mice that cannot tolerate 50 mg/L As were exposed to 0, 15, 20, 25 or30 mg/L As. iA s~Ⅲaccounted for 53% to 74% of total As in liver, pancreas, adipose, lung, heart, and kidney of As3mt-KO mice; tri- and pentavalent methylated arsenicals did not exceed 10% of total As. Tissues of WT mice retained iA s and methylated arsenicals: iA s~Ⅲ, MAs~Ⅲand DMAs~Ⅲ represented 55%‐68% of the total As in the liver, pancreas, and brain. High levels of methylated species, particularly MAs~Ⅲ, were found in the intestine of WT, but not As3mt-KO mice,suggesting that intestinal bacteria are not a major source of methylated As. Blood of WT mice contained significantly higher levels of As than blood of As3mt-KO mice. This study is the first to determine oxidation states of As species in tissues of As3mt-KO mice. Results will help to design studies using WT and As3mt-KO mice to examine the role of iA s methylation in adverse effects of iA s exposure. | Jenna M.Currier Christelle Douillet Zuzana Drobná Miroslav Styblo | 2016 | Journal of Environmental Sciences2016，28，11： | 8 |
| 8 | Characterization of three transcripts encoding small heat shock proteins expressed in the codling moth, Cydia pomonella (Lepidoptera: Tortricidae)显示文摘幼鳕蛾是苹果和世界范围的梨的一个主要害虫。这只昆虫怎么对环境应力作出回应的增加的知识将改进措施对它使用了的地和收获以后的控制。小热吃惊蛋白质(sHsps ) 在对环境 stressors 的细胞的回答起一个主要作用。一份退化 oligonucleotide 教材,对保存 -crystallin 领域设计了,在互补 DNA (cDNA ) 结束反应的 3 快速的扩大被使用放大编码在幼鳕蛾房间线表示的 sHsps 的抄本, Cp169,使遭到了加热吃惊。三全身的 cDNAs 从包含了编码 sHsps 的开的读物框架的 Cp169 房间被克隆。为 CpHsp19.8 的 cDNA 是编码 177 氨基酸的 795 bp。为 CpHsp19.9 的 cDNA 是编码 175 氨基酸的 749 bp。为 CpHsp22.2 的 cDNA 是编码 192 氨基酸的 737 bp。三 CpHsps 的蛋白质序列的分析与相同显示了 83 氨基酸的存在到 -crystallin 领域。为每 CpHsps, -crystallin 领域被分叉的 N 终端和 C 终端区域包围,与 sHsps 的保存结构的特征一致。实时聚合酶链反应,过去常决定每在幼鳕蛾的不同发展阶段的 sHsps 的表示模式在测试的所有阶段揭示了抄本的存在。与另外的 sHsps 的特征一致,当昆虫被使遭到加热吃惊时, CpHsp 抄本的表示极大地被提高。这研究的结果能被用作一个指南用各种各样的收获以后的处理在幼鳕蛾控制学习 sHsps 的角色。 | Stephen F. Garczynski Thomas R. Unruh Christelle Guedot Lisa G. Neven | 2011 | Insect Science2011，18，5： | 6 |
| 9 | 利用无机抗菌剂研制抗菌自酸蚀处理剂的初步研究显示文摘目的评价利用无机抗菌剂研制抗菌自酸蚀处理剂的初步可行性。方法将6种无机抗菌剂以0.5% w/v和2.5% w/v的浓度分别加入自行研制的实验自酸蚀处理剂ESP中,进行变形链球菌体外药敏试验。并应用添加了抗菌剂的ESP制备黏结样本,测试无机抗菌剂的添加对材料固化后抗菌活性的影响。结果 ESP本身就具有一定的抗菌作用,在其中添加龙贝无机抗菌粉体或氧化锌晶须复合抗菌剂AT-83可使液体状态下ESP的最小抑菌浓度和最小杀菌浓度显著降低;加入受试的无机抗菌剂使材料固化后的抗菌活性显著增强,其中添加氧化锌晶须复合抗菌剂AT-83的效果最为显著。结论从抗菌性能方面证实了利用受试的无机抗菌剂研制抗菌自酸蚀处理剂的初步可行性。 | 方明 Christel Neut 陈吉华 刘奕 赵三军 Hartmut F Hildebrand | 2007 | 北京口腔医学2007，15，1： | 6 |
| 10 | 纳滤去除水中内分泌干扰物双酚A和四溴双酚A的研究显示文摘用死端过滤法考察纳滤膜Desal5DK处理水中内分泌干扰物双酚A(BPA)和四溴双酚A(TBBPA)的效率,并研究吸附对纳滤截留有机分子的影响.结果表明,纳滤膜Desal5DK对BPA的表观截留率随累积吸附量的增加而降低,从初始的89%降至达到平衡状态时的47%,BPA累积吸附量相应达到约30μg·m-2.较高压力时,吸附层较高的溶质浓度引起膜通量下降.虽然Desal5DK对TBBPA累积吸附量达到50μg·m-2,但由于分子量较大和分子结构复杂,截留率较高(>95%).5×105Pa压力下解吸时TBBPA分子由于位阻效应几乎不能透过纳滤膜;而BPA则扩散通过纳滤膜,并且在流出30mL时浓度达到峰值,流出的BPA总量约占总吸附量的30%. | 张阳 胡锦英 李光哲 Christel Causserand Pierre Aimar | 2010 | 环境科学2010，31，6： | 6 |
| 11 | Canopy gap size influences niche partitioning of the ground-layer plant community in a northern temperate forest显示文摘Aims The Gap Partitioning Hypothesis(GPH)posits that gaps create heterogeneity in resources crucial for tree regeneration in closed-canopy forests,allowing trees with contrasting strategies to coexist along resource gradients.Few studies have examined gap partitioning of temperate,ground-layer vascular plants.We used a ground-layer plant community of a temperate deciduous forest in northern Wisconsin,USA,as a model system to test whether the GPH extends to the relatively species-rich ground layer.Methods We used a well-replicated experimental approach that included a gap opening gradient(five gap sizes,6,10,20,30 and 46 m diameter,and undisturbed reference areas),a within-gap location gradient(gap edge to center),and a temporal gradient(0,2,6 and 13 years after gap creation).The data were observations of ground-layer plant abundance,published plant traits,and a modeled index of understory light environments.We ordinated the plant abundance data and evaluated the relationships of composition,traits and light environment by gap size,location along the forest-gap transect and time,with several approaches such as correlations,descriptive statistics,non-parametric tests of group differences and indicator species importance values.Important Findings Ground-layer plant composition and traits differed across gap sizes,within-gap locations and over time.Gaps of all sizes differed in composition from undisturbed areas,and all pair-wise combinations of gap size also differed in composition,except the 6 m from the 10-m gaps.Large gaps(46 m)also displayed within-gap compositional gradients from gap edge to center locations.Compositional differences in gap size were evident 2 years after gap creation and,contrary to our hypotheses,remained different over the 13-year period,even in gaps with crown closure.In contrast to the neutral theory,species functional traits and microenvironmental conditions were related to variation in ground-layer composition.Species with smaller seeds,lower shade tolerance,later bloom times,shorter stature and longer leaves were associated with higher light,more central gap locations,larger gap sizes and greater time since gap creation.The correlation between gap size and ground-layer plant composition and traits provides evidence for gap partitioning by the diverse ground-layer community in this temperate deciduous forest community. | Christel C.Kern Rebecca A.Montgomery Peter B.Reich Terry F.Strong | 2013 | Journal of Plant Ecology2013，6，1： | 6 |
| 12 | Protective effects of estrogens and caloric restriction during aging on various rat testis parameters显示文摘瞄准:调查 17 β-estradiol (E2 ) 的效果, Peganum 伤害翼摘录(PHE ) 和各种各样的睾丸参数上的热量的限制(CR ) 在老化期间。方法:Twelve-month-old 雄的老鼠与 E2 或 PHE 被对待 6 个月,或提交了到 CR (40%) 。结果:我们的结果证明雌激素和 CR 能由象 aromatase 和雌激素受体基因表情的减少一样阻止睾丸激素和 E2 层次的减少保护男性腺。确实, E2, PHE 和 CR 处理在超级氧化物 dismutase 活动导致了增加并且减少阴囊的酶的活动:gamma-glutamyl transferase,碱的磷酸酶,喂奶 deshydrogenase 以及 aspartate 并且喂奶在年老的动物的 transaminases。另外,阴囊的过氧化氢酶和 gluthatione peroxidase 活动在 18 月年龄与未经治疗的动物相比在 E2, PHE 和对待 CR 的老鼠被提高。而且, estradiol, PHE 和 CR 的积极效果被类脂化合物的底层进一步每氧化支持。精子发生的恢复在对待的老鼠被记录。结论:除为精子发生有益的一本低热量的食谱以外,雌激素的一个保护的 antioxydant 角色被建议。雌激素推迟阴囊的房间损坏,它导致功能的老朽并且,因此雌激素在保护繁殖功能免受在年老的睾丸在大数量生产的反应的氧种类(ROS ) 施加的不利效果的伤害是有用的。 | Khaled Hamden Dorothee Silandre Christelle Delalande Abdelfattah E1Feki Serge Carreau | 2008 | Asian Journal of Andrology2008，10，6： | 5 |
| 13 | Single-cell profiling reveals the trajectories of natural killer cell differentiation in bone marrow and a stress signature induced by acute myeloid leukemia显示文摘Natural killer(NK)cells are innate cytotoxic lymphoid cells(ILCs)involved in the killing of infected and tumor cells.Among human and mouse NK cells from the spleen and blood,we previously identified by single-cell RNA sequencing(scRNAseq)two similar major subsets,NK1 and NK2.Using the same technology,we report here the identification,by single-cell RNA sequencing(scRNAseq),of three NK cell subpopulations in human bone marrow.Pseudotime analysis identified a subset of resident CD56^(bright) NK cells,NK0 cells,as the precursor of both circulating CD56dim NK1-like NK cells and CD56^(bright) NK2-like NK cells in human bone marrow and spleen under physiological conditions.Transcriptomic profiles of bone marrow NK cells from patients with acute myeloid leukemia(AML)exhibited stress-induced repression of NK cell effector functions,highlighting the profound impact of this disease on NK cell heterogeneity.Bone marrow NK cells from AML patients exhibited reduced levels of CD160,but the CD160high group had a significantly higher survival rate. | Adeline Crinier Pierre-Yves Dumas Bertrand Escalière Christelle Piperoglou Laurine Gil Arnaud Villacreces Frédéric Vély Zoran Ivanovic Pierre Milpied Émilie Narni-Mancinelli Éric Vivier | 2021 | Cellular & Molecular Immunology2021，18，5： | 5 |
| 14 | 布美他尼治疗3至6岁孤独症谱系障碍儿童后症状改善:一项随机、双盲、安慰剂对照的临床试验显示文摘孤独症谱系障碍是一大类婴幼儿期起病、以社交沟通和感知觉障碍为主要特征的神经发育障碍性疾病.目前治疗以康复为主,无有效药物.本研究采用随机、双盲、安慰剂对照临床试验策略,探索布美他尼干预对低龄孤独症患儿的疗效、安全性及潜在的神经生物学机制.研究结果显示,与安慰剂对照组相比,为期3个月的布美他尼药物干预可安全、有效改善低龄孤独症患儿核心症状,药效可能与其调节岛叶皮层GABA功能有关.本研究为目前国际上单站点最大样本量的随机双盲临床试验,研究结果为低龄孤独症患儿提供了潜在的药物治疗方案. | 戴媛 章玲丽 俞珏华 周欣 何华 季忆婷 Kai Wang 杜秀娟 刘欣 唐云 邓世宁 Christelle Langley 李伟广 张军 冯建峰 Barbara J.Sahakian 罗强 李斐 | 2021 | Science Bulletin2021，66，15： | 5 |
| 15 | The IgH 3′ regulatory region super-enhancer does not control IgA class switch recombination in the B1 lineage显示文摘The bone marrow-derived B2 population represents the vast majority of bone marrow,blood,lymph node and splenic B-cells.Mouse B1 B-cells mostly originate during embryonic life in the liver and represent the main B-cell population in the pleural and peritoneal cavities.1,2,3,4,5 B1 and B2 B-cells differ in their origin,antigen specificity,cell surface markers,tissue distribution and capacity for class switch recombination(CSR).Schematically,B1 B-cells appear earlier than B2 B-cells during fetal development and maintain their self-renewal ability throughout their life. | Hussein Issaoui Nour Ghazzaui Alexis Saintamand Claire Carrion Christelle Oblet Yves Denizot | 2018 | Cellular & Molecular Immunology2018，15，3： | 4 |
| 16 | Levels of insecticide resistance to deltamethrin,malathion,and temephos,and associated mechanisms in Aedes aegypti mosquitoes from the Guadeloupe and Saint Martin islands(French West Indies)显示文摘Background:In the Guadeloupe and Saint Martin islands,Aedes aegypti mosquitoes are the only recognized vectors of dengue,chikungunya,and Zika viruses.For around 40 years,malathion was used as a mosquito adulticide and temephos as a larvicide.Since the European Union banned the use of these two insecticide molecules in the first decade of the 21st century,deltamethrin and Bacillus thuringiensis var.israelensis are the remaining adulticide and larvicide,respectively,used in Guadeloupe.In order to improve the management of vector control activities in Guadeloupe and Saint Martin,we investigated Ae.aegypti resistance to and mechanisms associated with deltamethrin,malathion,and temephos.Methods:Ae.aegypti mosquitoes were collected from six different localities of Guadeloupe and Saint Martin.Larvae were used for malathion and temephos bioassays,and adult mosquitoes for deltamethrin bioassays,following World Health Organization recommendations.Knockdown resistance(Kdr)genotyping for V1016I and F1534C mutations,and expression levels of eight enzymes involved in detoxification mechanisms were examined in comparison with the susceptible reference Bora Bora strain.Results:Resistance ratios(RR50)calculated for Ae.aegypti larvae showed high resistance levels to temephos(from 8.9 to 33.1-fold)and low resistance levels to malathion(from 1.7 to 4.4-fold).Adult females displayed moderate resistance levels to deltamethrin regarding the time necessary to affect 50%of individuals,varying from 8.0 to 28.1-fold.Molecular investigations on adult mosquitoes showed high resistant allele frequencies for V1016I and F1534C(from 85 to 96%and from 90 to 98%,respectively),as well as an overexpression of the glutathione S-transferase gene,GSTe2,the carboxylesterase CCEae3a,and the cytochrome genes 014614,CYP6BB2,CYP6M11,and CYP9J23.Conclusions:Ae.aegypti populations from Guadeloupe and Saint Martin exhibit multiple resistance to organophosphates(temephos and malathion),and pyrethroids(deltamethrin).The mechanisms associated with these resistance patterns show strong frequencies of F1534C and V1016I Kdr mutations,and an over-expression of CCEae3a,GSTe2,and four cytochrome P450 genes(014614,CYP9J23,CYP6M11,CYP6BB2).These results will form the baseline for a deeper understanding of the insecticide resistance levels and associated mechanisms of Ae.aegypti populations and will be used to improve vector control strategies in Guadeloupe and Saint Martin. | Daniella Goindin Christelle Delannay Andric Gelasse Cedric Ramdini Thierry Gaude Frederic Faucon Jean-Philippe David Joël Gustave Anubis Vega-Rua Florence Fouque | 2017 | Infectious Diseases of Poverty2017，6，1： | 3 |
| 17 | 抗生素耐药性环境中产生和转移的人类健康风险评估(HHRA)显示文摘[背景]直到最近,人们才明确环境能影响抗生素耐药性风险对临床结果的影响,但迄今为止,很少有文献记录正式评估这些风险的方法。[目标]我们研究可能的方法,并试图确定人类健康风险评估(HHRA)的研究需求,这项评估注重环境在抗生素耐药性病原体所致的抗生素治疗失败中所起的作用。[方法]作者参加了2012年3月4—8日在加拿大魁北克省举行的研讨会,定义抗生素耐药性风险与人类健康环境评估的范围和目标。我们专注于环境中耐药性产生'热点区域'的关键要素,(与食品无关的)暴露评估以及剂量反应,以描述风险特征,从而改善抗生素耐药性管理的方案。[讨论]识别传统风险评估中有助于评估环境中抗生素耐药性的各个新方面。包括:a)解释附加的选择压力对环境耐药基因组的作用,即随着时间的推移,促使抗生素耐药性细菌(ARB)产生;b)在相关的环境组成部分的'热点区域'中识别和描述水平基因转移(HGT)率;c)针对不同健康结局和途径的ARB剂量修改传统的剂量反应方法。[结论]我们建议将抗生素耐药性产生造成的环境影响纳入所有涉及ARB的HHRA过程之中。由于可用的数据有限,一种多标准决策分析方法将有助于进行环境中抗生素耐药性的HHRA,并使风险管理者了解环境抗生素耐药性。 | Nicholas J.Ashbolt Alejandro Amézquita Thomas Backhaus Peter Borriello Kristian K.Brandt Peter Collignon Anja Coors Rita Finley William H.Gaze Thomas Heberer John R.Lawrence D.G.Joakim Larsson Scott A.McEwen James J.Ryan Jens Schnfeld Peter Silley Jason R.Snape Christel Van den Eede Edward Topp 王晓宇 张伊人 操仪 | 2014 | 环境与职业医学2014，31，2： | 3 |
| 18 | Antimicrobial resistance of abnormal vaginal discharges microorganisms in Ouagadougou,Burkina Faso显示文摘Objective:To assess the prevalence of bacterial strains and fungal strains infecting the vaginal tract and test their sensitivity to antibiotics in women attending Saint Camille Medical Centre in Ouagadougou.Methods:From January 2008 to December 2009,a total of 2000 vaginal swabs were cultivated for bacterial and fungal identification and isolation.Furthermore,bacterial strains were tested for their susceptibility to several antibiotics used in routine in the centre. Results:The results revealed that microbial isolation and identification was attempted for 1536/2000 sample,a positivity rate of 76.80%.Candida albicans(48.76%),followed by Escherichia coli(16.67%),Streptococcus agalactiae(8-14%) and Staphylococcus aureus(7.55%) were the major agents of genital tract infections in patients.Mycoplasma hominis and Ureaplasma urealyticum combined accounted for less than 7%.Trichomonas vaginalis was identified in 1.04%coses.The antimicrobial tests revealed that the microorganisms developed resistance to several antibiotics including beta lactams.However,antibiotics such as cefamenzol,ciprofloxacin and norfloxacin were still active on these bacteria.Conclusions:The results reveal that many sexually active women are infected by one or more microbial pathogens,probably because of the lock of hygiene or the adoption of some risky behaviors,such as not using condoms or having multiple sexual partners.Efforts should be made to address these points in the country. | Simplice D Karou Florencia Djigma Tani Sagna Christelle Nadembega Moctar Zeba Aboudoulaye Kabre Kokou Anani Djeneba Ouermi Charlemagne Gnoula Virginio Pietra Salvatore Pignatelli Jacques Simpore | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，4： | 2 |
| 19 | Hollow mesoporous titania microsphere with low shell thickness/diameter ratio and high photocatalysis显示文摘 | Jianing Liu Gaowen Zhang Wei Ao Kan Yang Shaoxian Peng Christel Müller-Goymann | 2012 | Applied Surface Science2012，，20： | 2 |
| 20 | Sclareol and linalyl acetate are produced by glandular trichomes through the MEP pathway显示文摘Sclareol,an antifungal specialized metabolite produced by clary sage,Salvia sclarea,is the starting plant natural molecule used for the hemisynthesis of the perfume ingredient ambroxide.Sclareol is mainly produced in clary sage flower calyces;however,the cellular localization of the sclareol biosynthesis remains unknown.To elucidate the site of sclareol biosynthesis,we analyzed its spatial distribution in the clary sage calyx epidermis using laser desorption/ionization mass spectrometry imaging(LDI–FTICR-MSI)and investigated the expression profile of sclareol biosynthesis genes in isolated glandular trichomes(GTs).We showed that sclareol specifically accumulates in GTs’gland cells in which sclareol biosynthesis genes are strongly expressed.We next isolated a glabrous beardless mutant and demonstrate that more than 90%of the sclareol is produced by the large capitate GTs.Feeding experiments,using 1-13 C-glucose,and specific enzyme inhibitors further revealed that the methylerythritol-phosphate(MEP)biosynthetic pathway is the main source of isopentenyl diphosphate(IPP)precursor used for the biosynthesis of sclareol.Our findings demonstrate that sclareol is an MEP-derived diterpene produced by large capitate GTs in clary sage emphasing the role of GTs as biofactories dedicated to the production of specialized metabolites. | Camille Chalvin Stephanie Drevensek Françoise Gilard Caroline Mauve Christel Chollet Halima Morin Edith Nicol Eva Heripre Lucie Kriegshauser Bertrand Gakiere Michel Dron Abdelhafid Bendahmane Adnane Boualem | 2021 | Horticulture Research2021，8，1： | 2 |
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