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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | Phylogenetic and genetic characterization of a 2017 clinical isolate of H7N9 virus in Guangzhou,China during the fifth epidemic wave显示文摘Pathogenic H7N9 influenza viruses continue to pose a public health concern. The H7N9 virus has caused five outbreak waves of human infections in China since 2013. In the present study, a novel H7N9 strain(A/Guangdong/8 H324/2017) was isolated from a female patient with severe respiratory illness during the fifth wave of the 2017 H7N9 epidemic. Phylogenetic analysis showed that the H7N9 viruses collected during the fifth wave belong to two different lineages: the Pearl River Delta lineage and the Yangtze River Delta lineage. The novel isolate is closely related to the Pearl River Delta H7N9 viruses, which were isolated from patients in Guangdong Province. The novel H7N9 isolate has an insertion of three basic amino acids in the cleavage site of hemagglutinin(HA), which may enhance virulence in poultry. The 2017 isolate also possesses an R292 K substitution in the neuraminidase(NA)protein, which confers oseltamivir resistance. This study highlights the pandemic potential of the novel H7N9 virus in mammals;thus, future characterization and surveillance is warranted. | Yongqiang Deng Chunlin Li Jianfeng Han Yingfen Wen Jian Wang Wenxing Hong Xiaofeng Li Zhongyu Liu Qing Ye Jing Li Changshuai Zhou Lei Yu Chengfeng Qin Fuchun Zhang Tao Jiang | 2017 | Science China(Life Sciences)2017，60，12： | 9 |
| 3 | Study of the Surface Morphology of Gas Hydrate显示文摘Study of the surface morphology of gas hydrate is of great importance in understanding its physical properties and occurrence.In order to investigate the surface morphology of different types(sI and sII)and occurrences(pore-filling and fracture-filling)of gas hydrate,both lab-synthesized and drilled-gas hydrate samples were measured using cryo-scanning electron microscopy(cryo-SEM).Results showed that the surface of s I hydrate was relatively smooth,and spongy nano-pores(200–400 nm)gradually occurred at the surface during continuous observation.The surface of sII hydrate was more compact,showing a tier-like structure.Hydrate occurred in quartz sand and usually filled the pores of the sediments and both hydrate and sediments were cemented with each other.SEM observation of the gas hydrates collected from the South China Sea showed that the surface morphology and contact relation with sediments varied with hydrate occurrence.For instance,hydrates dispersed in sediments mainly filled the pores of the sediments.The existence of microorganism shells,such as foraminifera,was beneficial to the formation of gas hydrate.When hydrate occurred as a massive or vein structure,it was easily distinguished from the surrounding sediments.The surface of hydrate with massive or vein structure showed two distinct characters:one was dense and smooth,the other is porous(several to tens of micrometers in diameter).The occurrence of different hydrate morphologies was probably caused by the supplement rates of methane gas. | SUN Jianye LI Chengfeng HAO Xiluo LIU Changling CHEN Qiang WANG Daigang | 2020 | Journal of Ocean University of China2020，19，2： | 9 |
| 4 | Influence of foraminifera on formation and occurrence characteristics of natural gas hydrates in fine-grained sediments from Shenhu area, South China Sea显示文摘Marine gas hydrates accumulate primarily in coarse-grained, high-permeability layers; however, highly saturated natural gas hydrates have been discovered in the fine-grained sediments of Shenhu area, South China Sea(SCS). This may be explained by key factors, such as the great abundance of foraminifera shells. In this paper, by analyzing the SCS foraminifera structure and performing hydrate formation experiments in the foraminifera shells, the contribution of foraminifera to hydrate accumulation in the SCS was investigated from a microscopic point of view. Simulations of hydrate formation were carried out in both pure SCS foraminifera shells and the host sediments. Pore structures in typical foraminifera were studied by use of micro-focus X-ray computed tomography(CT) and scanning electron microscopy(SEM). Hydrate growth and occurrence characteristics in the foraminifera shells were observed in-situ. The results showed that the presence of foraminifera significantly enhanced the effective porosity of the SCS sediments. Moreover, while the hydrates grew preferentially in the chambers of the coarse-grained foraminifera by adhering to the inner walls of the foraminifera shells, no apparent hydrate accumulation was observed in the fine-grained or argillaceous matrix. These findings provide a basis for further studies on the accumulation mechanism of hydrates and physical properties of hydrate reservoir in the South China Sea. | LI ChengFeng HU GaoWei ZHANG Wei YE YuGuang LIU ChangLing LI Qing SUN JianYe | 2016 | Science China Earth Sciences2016，59，11： | 7 |
| 5 | 寨卡病毒引起小鼠睾丸损伤并导致雄性不育显示文摘虽然寨卡病毒与其他黄病毒属成员,如登革热病毒、乙型脑炎病毒和两尼罗病毒都是通过蚊媒传播,但是前人的研究发现寨卡病毒在精液中存在数月之久,暗示其性接触传播的可能性。本研究表明寨卡病毒影响雄性生殖系统,提示了其对男性健康的潜在危害。 | Wenqiang Ma Shihua Li Shuoqian Ma Lina Jia Fuchun Zhang Yong Zhang Jingyuan Zhang Gary Wong Shanshan Zhang Xuancheng Lu Mei Liu Jinghua Yan Wei Li Chuan Qin Daishu Han Chengfeng Qin Na Wang 李向东 高福 | 2017 | 科学新闻2017，19，4： | 5 |
| 6 | Crystalline behaviors and phase transition during the manufacture of fine denier PA6 fibers显示文摘Recently we have successfully produced fine denier PA6 fibers by using additives containing lanthanide compounds.Meanwhile,crystallization and phase transition of PA6 fibers during spinning and drawing processes were investigated.During the spinning process,β phase crystal could be obtained in as-spun PA6 fibers which were produced with relatively high melt draw ratio,while γ phase crystal predominated when the melt draw ratio was relatively low.β phase crystal,whose behaviors are similar with those of γ phase by FT-IR and XRD characterization,could be transformed to α form easily when PA6 fibers are immersed in boiling water.However,γ phase crystal of PA6 remains unchanged in boiling water.Thus,β and γ phase crystals of PA6 can be differentiated by the crystalline behaviors of PA6 fibers after treatment in boiling water.Further experiments demonstrate that the β phase can also be produced during a drawing process where a phase transformation from γ to α occurs.In other words,β phase may act as an intermediate state during the phase transformation. | ZHANG ChengFeng LIU YuHai LIU ShaoXuan LI HuiZhen HUANG Kun PAN QingHua HUA XiaoHui HAO ChaoWei MA QingFang LV ChangYou LI WeiHong YANG ZhanLan ZHAO Ying WANG DuJin LAI GuoQiao JIANG JianXiong XU YiZhuang WU JinGuang | 2009 | Science China Chemistry2009，52，11： | 4 |
| 7 | Distribution of airborne SARS-CoV-2 and possible aerosol transmission in Wuhan hospitals, China显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused an outbreak of CO V ID-19(2019 coronavirus infectious disease)that has led to a global public health crisis.The spread of SARS-CoV-2 can be rapid;but there is still considerable controversy about its main route of transmission[l,2];that is,whether transmission is by contact or through the air[2,3].Recently the W H O(World Health Organization)released a document that recognized the possibility of aerosol transmission of SARS-CoV-2 for indoor environments and enclosed spaces[4].The purpose of the present study was to evaluate the airborne transmission of SARS-CoV-2 through a field investigation of its occurrence in the air and other samples collected from health facilities in W uhan;China. | Jia Hu Chengfeng Lei Zhen Chen Weihua Liu Xujuan Hu Rongjuan Pei Zhengyuan Su Fei Deng Yu Huang Xiulian Sun Junji Cao Wuxiang Guan | 2020 | National Science Review2020，7，12： | 4 |
| 8 | A Glycal Approach to the Synthesis of Pregnane Glycoside P57显示文摘Pregnane glycoside P57 is known as an active component of Hoodia gordonii, which has long been used as a diet with appetite suppressantproperty. Chemical synthesis of P57 demands installation of β-cymarosyl linkage onto the steroid aglycone. The stereoselective formation of this specia1 2-deoxy-β-glycosidic linkage has previously been proven to be difficult and thus a limiting step in the synthesis of P57 and its congeners. Herein, we reportthe glycosylation reactions with glycals as donors and TPHB (triphenylphosphine hydrobromide) as promoter and a convergent synthesis of P57 via a β-selective glycosylation with a trisaccharide glycal donor. | Chao Liu Yuyong Ma Chengfeng Pei Wei Li Biao Yu | 2018 | Chinese Journal of Chemistry2018，36，11： | 3 |
| 9 | Classification of CBERS-2 Imagery with Fuzzy ARTMAP Classifier显示文摘A fuzzy ARTMAP classifier is adopted for a classification experiment of CBERS-2 imagery. The fundamental theory and processing about the algorithm are first introduced, followed with a land-use classification experiment in Shihezi County on CBERS-2 high resolution imagery. Three classifiers are compared: maximum likelihood classifier (MLC), error back propagation (BP) classifier, and fuzzy ARTMAP classifier. The comparison shows comparably better results for the fuzzy ARTMAP classifier, with overall classification accuracy of 9.9% and 4.6% higher than that of MLC and BP. The results also prove that the fuzzy ARTMAP classifier has better discernment in identifying bare soil on CBERS-2 imagery. | LUO Chengfeng LIU Zhengjun YAN Qin | 2007 | Geo-Spatial Information Science2007，10，2： | 3 |
| 10 | Effect of Methane Gas on Acoustic Characteristics of Hydrate-Bearing Sediment–Model Analysis and Experimental Verification显示文摘Gas leakage is an important consideration in natural systems that experience gas hydrate accumulation.A number of velocity models have been created to study hydrate-bearing sediments,including the BGTL theory,the weighted equation,the Wood equation,the K-T equation,and the effective medium theory.In previous work,we regarded water as the pore fluid,which meant its density and bulk modulus values were those of water.This approach ignores the presence of gas,which results in a biased calculation of the pore fluid's bulk modulus and density.To take into account the effect of gas on the elastic wave velocity,it is necessary to recalculate the bulk modulus and density of an equivalent medium.Thus,a high-pressure reactor device for simulating leakage systems was developed to establish the relationship between wave velocity and hydrate saturation in methane-flux mode.A comparison of the values calculated by the velocity model with the experimental data obtained in this study indicates that the effective medium theory(EMT,which considers gas effects)is more applicable than other models.For hydrate saturations of 10%–30%,the result ranges between EMT-B(homogenous gas distribution)and EMT-B(patchy gas distribution).For hydrate saturations of 30%–60%,the results are similar to those of the EMT-B(homogenous gas distribution)mode,whereas hydrate saturations of 60%–70%yield results similar to those of the EMT-A mode.For hydrate saturations greater than 80%,the experimental results are similar to those of the EMT-B mode.These results have significance for hydrate exploitation in the South China Sea. | BU Qingtao HU Gaowei LIU Changling DONG Jie XING Tongju SUN Jianye LI Chengfeng MENG Qingguo | 2021 | Journal of Ocean University of China2021，20，1： | 2 |
| 11 | Targeting EZH1/2 induces cell cycle arrest and inhibits cell proliferation through reactivation of p57CDKN1C and TP53INP1 in mantle cell lymphoma显示文摘Objective: To explore the effect of dysregulation of epigenetic regulator EZH1 and EZH2 on the proliferation in MCL and the underlying mechanisms.Methods: In this study, we elucidated the role of EZH1 and EZH2 overexpression by immunohistochemistry and correlated them to clinical outcome in 41 MCL patients.Quantitative real-time PCR and Western blot were applied to confirm the level of EZH1 and EZH2 in well-characterized MCL cell lines which were compared to those of na?ve B cells.Then we manipulated the expression of EZH1 and EZH2 in MCL cells using CRISPR/Cas9 system to directly investigate their functional roles in MCL.We also evaluated the effect of two small molecule selective inhibitors, EPZ005687 and UNC1999, on MCL cell proliferation, cell cycle distribution and apoptosis in vitro.Finally, we performed RNA-sequencing(RNA-Seq) and Chromatin immunoprecipitation(ChIP) assay to further gain insight into the underlying molecular mechanisms.Results: We found that EZH2 protein is overexpressed in approximately half of this cohort of MCL cases.More importantly, the overexpression of EZH2 is associated with poor OS in the patients.Nevertheless, simple EZH2 depletion in vitro has little impact on the viability of MCL cells, predominantly because of the consequent up-regulation of EZH1.Consistently, UNC1999, a dual EZH1/2 inhibitor, unlike the EZH2 selective inhibitor EPZ005687, exerts a potent inhibitory effect on MCL cells.Furthermore, we discover CDKN1C and TP53 INP1 as the two important cell cycle regulators, the expression of which are repressed by EZH1/2 mediated epigenetic regulation and are restored by EZH1/2 dual inhibition.Conclusions: Our study suggests that EZH2 participates in the pathogenesis of MCL which may serve as a potential biomarker for prognosis prediction.The dual inhibition of EZH1/2 is a promising therapeutic strategy for MCL. | Wei Li Chengfeng Bi Yating Han Tian Tian Xianhuo Wang Huijing Bao Xiaoying Xu Xuhan Zhang Lu Liu Weiwei Zhang Hai Gao Huaqing Wang Huilai Zhang Bin Meng Xi Wang Kai Fu | 2019 | Cancer Biology & Medicine2019，16，3： | 2 |
| 12 | Tribologi- cal performance of room-temperature ionic liquids as lubricant 显示文摘 | Liu Weimin Ye Chengfeng Gong Qingye | 2002 | TribologyLetters2002，13，2： | 1 |
| 13 | Room-tem- perature ionic liquids: A novel versatile lubricant显示文摘 | Ye Chengfeng Liu Weimin Chen Yunxia | 2001 | Chemi- cal Communications2001，，21： | 1 |
| 14 | Mesoporous metal–organic framework materials显示文摘 | XUAN Weimin ZHU Chengfeng LIU Yan | 2012 | Chem Soc Rev2012，41，： | 1 |
| 15 | Room-tem-perature ionic liquids: A novel versatile lubricant显示文摘 | Ye Chengfeng Liu Weimin Chen Yunxia et aI | 2001 | Chemi- cal Communications2001，21，21： | 1 |
| 16 | Tribological Performance of Room-Temperature Ionic Liquids as Lubricant显示文摘 | Weimin Liu Chengfeng Ye Qingye Gong Haizhong Wang Peng Wang | 2002 | Tribology Letters2002，，2： | 1 |
| 17 | Tribological performance of room-temperature ionic liquids as lubricant 显示文摘 | Liu Weimin Ye Chengfeng Gong Qingye | 2002 | Tribology Letters2002，13，2： | 1 |
| 18 | Experimental demonstration of induced - transparency based on a novel re- sonator system 显示文摘 | Cui Danfeng Xie Chengfeng Liu Yaoying | 2014 | Optics Communications2014，324，4： | 1 |
| 19 | Hydrothermal synthesis of large-sized hydroxyapatite whiskers regulated by glutamic acid in solutions with low supersaturation of precipitation显示文摘 | Chengfeng Li Shuguang Liu Guochang Li Jiahai bai Weiwei Wang Qingyang Du | 2010 | Advanced Powder Technology2010，，4： | 1 |
| 20 | Spherical hydroxyapatite with colloidal stability prepared in aqueous solutions containing polymer/surfactant pair显示文摘 | Chengfeng Li Guochang Li Shuguang Liu Jiahai bai Aijuan zhang | 2010 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2010，，1： | 1 |
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