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| 1 | Protective effects of Buyinqianzheng Formula on mitochondrial morphology by PINK1/Parkin pathway in SH-SY5Y cells induced by MPP^(+)显示文摘Objective:Buyinqianzheng Formula(BYQZF)is clinically employed in traditional Chinese medicine to treat Parkinson's disease(PD)by improving mitochondrial dysfunction.However,the underlying mechanisms by which BYQZF affects mitochondrial morphology remain unknown.Therefore,we observed the effects of BYQZF on mitochondria from the perspective of the PINK1/Parkin pathway.Methods:Cell survival rates were assessed by Cell Counting Kit-8 assay.Expression levels of PINK1 and Parkin mRNA were examined by qRT-PCR.Protein expression levels of PINK1,PINK1-Ser228,Parkin,Parkin-Ser65,Drp1,and Drp1-Ser637 were examined by western blotting.PINK1,Parkin,and MitoTracker?Red CMXRos(MTR)were stained by triple-labeled immunofluorescence,and observed under laser confocal microscopy.Results:Cell survival rate,mitochondrial form factor,mean length and number of mitochondrial network branches,mitochondrial activity,m RNA expression levels of PINK1 and Parkin,and protein expression levels of PINK1,Parkin,and Drp1-Ser637 were reduced after 1-methyl-4-phenylpyridinium(MPP^(+))intervention.In contrast,Pearson's correlation coefficients between PINK1 and Parkin,and between Parkin and MTR,as well as protein expression levels of PINK1-Ser228,Parkin-Ser65,and Drp1 increased significantly after MPP^(+) intervention.Treatment with BYQZF increased cell survival rate,mitochondrial form factor,mean length and number of mitochondrial network branches,mitochondrial activity,m RNA expression levels of PINK1 and Parkin,and expression of PINK1,Parkin,and Drp1-Ser637 proteins.Pearson's correlation coefficients between PINK1 and Parkin,and between Parkin and MTR,as well as protein expression levels of PINK1-Ser228,Parkin-Ser65,and Drp1 decreased after BYQZF treatment.Conclusion:These results demonstrate that BYQZF has a protective effect on mitochondrial molecular mechanisms in the PD cell model,and the mechanism is related to the PINK1/Parkin pathway. | Haojie Ma Zhenyu Guo Cong Gai Cuicui Cheng Jinkun Zhang Yuxin Zhang Luping Yang Wandi Feng Yushan Gao Hongmei Sun | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，3： | 3 |
| 2 | Mechanism of mitochondrial protection by the Buyin Qianzheng formula in a Parkin overexpression cell model显示文摘Objective:To identify the molecular mechanisms of the effects of the Buyin Qianzheng formula(BYQZF)on the mitochondrial dynamics in a Parkin overexpression Parkinson's disease(PD)cell model.Methods:First,a stable Parkin overexpression cell model was constructed using plasmid transfection.Then,we examined the protective effect of BYQZF on the mitochondrial dysfunction of the Parkin overexpression PD cell model induced by neurotoxin 1-methyl-4-phenylpyridinium ion(MPPþ).The mRNA expression level of Parkin was evaluated using real-time quantitative PCR.The cell survival rate was detected using the Cell Counting Kit-8 assay.We evaluated the cellular adenosine triphosphate(ATP)levels using luciferase assays.A laser scanning confocal microscope was used to observe the mitochondrial morphology,activity,and mitochondrial membrane potential(DJm).Western blot was conducted to evaluate the levels of the fusion proteins mitofusin1,mitofusin2,optic atrophy 1,dynaminrelated protein 1,and mitochondrial fission protein 1.Results:Parkin overexpression attenuated MPPþ-induced mitochondrial damage,increased mitochondrial activity and DJm.BYQZF increased the survival of MPPþ-induced cells that overexpressed Parkin and upregulated the mitochondrial form factor and activity.It also inhibited a decrease in the DJm and ATP levels.These findings suggested that BYQZF protected against MPPþ-induced mitochondrial dysfunction and enhanced the protective effect of Parkin overexpression.Furthermore,the formula upregulated the expression of the fusion proteins mitofusin1,mitofusin2,and optic atrophy 1(closely related to mitochondrial quality remodeling),and reduced the expression of the fission protein dynamicrelated protein 1,as well as mitochondrial fission protein 1.Conclusion:The mechanism by which BYQZF increased the mitochondrial protective effect of Parkin gene overexpression in MPPþ-induced cells may be related to improving mitochondrial function and regulating the balance of mitochondrial division and fusion proteins. | Cuicui Cheng Yushan Gao Cong Gai Wandi Feng Haojie Ma Jing Feng Zhenyu Guo Shujing Zhang Jie Wu Hongmei Sun | 2022 | Journal of Traditional Chinese Medical Sciences2022，9，1： | 0 |
| 3 | The construction of OPA1 overexpression plasmid and the cell model显示文摘The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected with the OPA1 overexpression plasmid by Lipo3000 transfection reagent in liposome transduction technology.A total of 3 groups were set up:the control group,negative control group,and OPA1 overexpression model group.RT-PCR technique was used to detect the expression of OPA1 at mRNA level.In addition,Western blot technique was used to detect the expression of OPA1 at protein level.We found that the mRNA and protein expression of OPA1 were significantly increased after transfection with OPA1 overexpression plasmid.These results highlight using liposome transduction technology,the OPA1 overexpression plasmid was successfully transfected into SH-SY5Y cells by Lipo3000 and the cell model of OPA1 gene overexpression was successully established. | Ma Haojie Gai Cong Feng Wandi Cheng Cuicui Zhou Mengqi Zhang Jinkun Zhang Yuxin Yang Luping Guo Zhenyu Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 4 | Mechanism of Buyin Qianzheng Fang on preventing dopamine neuron from apoptosis by regulating intracellular Ca^(2+) in PD Mice显示文摘The purpose of this study is to investigate the possible protective mechanism of Buyin Qianzheng Fang(BYQZF)on endoplasmic reticular and mitochondrial function through intracellular Ca^(2+) to protect dopamine neurons in Parkinson's disease mice model.C57BL/6 mice were randomly divided into normal group,model group,BYQZF group.The behavior of mice was observed by climbing and beam hang tests. | Zhao Haihong Feng Wandi Ma Haojie Cheng Cuicui Zhou Mengqi Gao Yushan Gai Cong Sun Hongsun | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 5 | Effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in PD cell model显示文摘Explore the effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in Parkinson's disease(PD)cell model,and lay the foundation for screening and mechanism research of anti-Parkinson's disease drugs.The PD cell model was constructed by applying MPP+to SH-SY5Y cells for 48 h,and they were treated with low,.medium and high concentrations of Timosaponin B-Ⅱ. | Zhou Mengqi Gai Cong Feng Wandi Ma Haojie Cheng Cuicui Zhao Haihong Zhang Yuxin Zhang Jinkun Yang Luping Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 6 | High sensitivity and broad linearity range pressure sensor based on hierarchical in-situ filling porous structure显示文摘Flexible piezoresistive pressure sensor with high sensitivity over a broad linearity range have been attracting tremendous attention for its applications in health monitoring,artificial intelligence,and human-machine interfaces.Herein,we report a hierarchical insitu filling porous piezoresistive sensor(HPPS)by direct ink writing(DIW)printing and curing of carbon nanofibers(CNFs)/polydimethylsiloxane(PDMS)emulsion.Hierarchical geometry significantly increases the contact area,distributes stress to multilayered lattice and internal porous structure,resulting in a broad sensing range.Moreover,unlike conventional hollow porous structure,the CNFs networks in-situ filling porous structure generates more contact sites and conductive pathways during compression,thereby achieving high sensitivity and linearity over entire sensing range.Therefore,the optimized HPPS achieves high sensitivity(4.7 kPa^(−1))and linearity(coefficient of determination,R^(2)=0.998)over a broad range(0.03-1000 kPa),together with remarkable response time and repeatability.Furthermore,the applications in diverse pressure scenarios and healthcare monitoring are demonstrated. | Jin Xu Huayang Li Yiming Yin Xin Li Jinwei Cao Hanfang Feng Wandi Bao Hao Tan Fanyuan Xiao Guang Zhu | 2022 | npj Flexible Electronics2022，6，1： | 0 |
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