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| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | EXPERIMENTAL INVESTIGATION AND COMPUTER SIMULATION ON DYNAMIC BUCKLING BEHAVIOR OF LIQUID-FILLED CYLINDRICAL SHELLS UNDER AXIAL IMPACT显示文摘This article reports an experimental investigation on the axial impact buckling of thin metalliccylindrical shells fully filled with water.Low velocity impact tests are carried out by DHR-9401 drop hammerrig.The whole process of dynamic buckling is simulated using LS-DYNA computer code.The consistencybetween experimental observation and numerical simulation is quite satisfactory.The investigation indieatesthat quite high internal hydrodynamic pressure occurs inside the shell during the impact process.Under thecombined action of the high internal pressure and axial compression plastic buckling occurs easily in the thin-walled shells and buckling modes take on regular and axisymmetric wrinkles. | Zhang, SY Lei, JP Zhao, LM Cheng, GQ Lu, GY | 2000 | Acta Mechanica Solida Sinica2000，13，2： | 2 |
| 3 | Effet of Maotai liguor in inducing metallothioneins and on hepatic stellate cells显示文摘 | Cheng ML Wu J Wang HQ Xue LM Tan YZ Liu P Li CX Huang NH Yao YM Ren LZ Ye L Li L Jia ML | 2001 | Shijie Huaren Xiaohua Zazhi2001，9，12： | 2 |
| 4 | Drug-metabolizing enzyme and transporter expression in a mouse model of diabetes and obesity显示文摘 | Cheng Q Aleksunes LM Manautou JE Cherrington NJ Scheffer GL Yamasaki H | 2008 | Mol Pharm2008，5，1： | 1 |
| 5 | Quantitative sensory testing risk factor of diabetic sensory neuropathy 显示文摘 | Cheng WY Jiang YD Chang LM | 1999 | J Neurol1999，246，5： | 1 |
| 6 | Expression of cyclooxygenase-2 in prostate adenocarcinoma and benign prostatic hyperplasia 显示文摘 | Lee LM Pan C C Cheng C J | 2001 | Anticancer Res2001，21，： | 1 |
| 7 | Guillain - Barre syndrome in Taiwan:a clinical study of 167 patients 显示文摘 | Lyu RK Tang LM Cheng SY | 1997 | J Neurol Neurosurg Psychiatry1997，63，： | 1 |
| 8 | Is the volume of distribution of digoxin reduced in patients with renal dysfunction? Determining digoxin pharmacokinetics by fluorescence polarization immunoassay 显示文摘 | Cheng JW Charland SL Shaw LM | 1997 | Pharmacotherapy1997，17，3： | 1 |
| 9 | Pedicle screw fixation for traumatic fractures of the thoracic and lumbar spine显示文摘 | Cheng LM Wang JJ Zeng ZL | 2013 | Cochrane Database Syst Rev2013，5，： | 1 |
| 10 | An outbreak of coxsackievirus A16 infection: comparison with other enteroviruses in a preschool in Taipei 显示文摘 | Wu PC Huang LM Kao CL Fan TY Cheng AL Chang LY | 2010 | J Microbiol Immunol Infect2010，43，4： | 1 |
| 11 | Drug-metabolizing enzyme and transporter expres-sion in a mouse model of diabetes and obesity 显示文摘 | Cheng Q Aleksunes LM Manautou JE | 2008 | Mol Pharm2008，5，1： | 1 |
| 12 | KIT gene mutation and amplification in dysgerminoma of the ovary显示文摘 | Cheng L Roth LM Zhang S | | 0，，10： | 1 |
| 13 | Orthogonal experiment of optimization astr- galoside water extraction 显示文摘 | Wang SM Cheng XW Wu LM | 2006 | Sinopharm Medicine Shizhen2006，17，12： | 1 |
| 14 | Correlates of echocardiogTaphic indices of cardiac remodeling over the adult life course: longitudinal observations from the Framingham Heart Study显示文摘 | Cheng S Xanthakis V Sullivan LM | 2010 | Circulation2010，122，6： | 1 |
| 15 | OCT4: a novel biomarker for dysgerininoina of the ovatT显示文摘 | Cheng L Thomas A Roth LM | 2004 | Am J Surg Pathol2004，28，10： | 1 |
| 16 | Vocal tract dimensional develop-ment of adolescents:an acoustic reflection study显示文摘 | Xue SA Cheng RW Ng LM | | 0，，08： | 1 |
| 17 | Herbs of the genus Phyllanthus in the treatment of chromic hepatitis B: observations with three preparations from different geographic sites显示文摘 | Wang MX Cheng HW Li YJ Meng LM Zhao GL Mai K | 1995 | Journal of Laboratory and Clinical Medicine1995，126，4： | 1 |
| 18 | OCT4: a novel biomarker for dysgerminoma of the ovary 显示文摘 | Cheng L Thomas A Roth LM | 2004 | Am J Sug Pathol2004，28，10： | 1 |
| 19 | Drug-metabolizing enzyme and transporter expres sion in a mouse model of diabetes and obesity显示文摘 | Cheng Q Aleksunes LM Manautou JE | 2008 | Mol Pharmaceutics2008，5，1： | 1 |
| 20 | Discrepancies between two automated immunoassay systems in determining hepatitis B virus markers in serum samples with concomitant presence of antigens and antibodies显示文摘 | Cheng LM Guan Q Zhang JX | 2010 | Ann Clin Lab Sci2010，40，1： | 1 |
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