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| 1 | 间质干细胞移植修复脊髓损伤:从基础研究到临床转化显示文摘脊髓损伤是一类通常由创伤所致感觉和运动功能受损的临床常见疾病,其病理生理改变主要发生初损伤过程及二次损伤过程,可直接导致器官功能障碍、组织坏死、感觉和运动功能的不可逆病变,甚至死亡的严重后果。为抑制二次损伤和修复受损的中枢神经系统,国内、外开展了广泛的探索与尝试,修复手段包括药物治疗、手术减压、生物材料移植、自体外周神经移植等方法,但临床疗效并不满意。干细胞具有可促进神经细胞再生及直接分化成神经样细胞的可能,同时具有抗炎、抗凋亡、抗氧化应激、促分泌营养因子及促血管再生等多重疗效,因此干细胞移植修复脊髓损伤近年来为脊髓损伤患者带来了新的希望。在众多类型的干细胞中,间质干细胞由于其具有机体内分布广、分化潜能强、分离难度低、体外保存扩增易操作及可避免伦理道德等特点,并已在体外、动物和临床等不同层面进行了一系列尝试,取得了一定的成果。近年来,间质干细胞移植在脊髓损伤的修复研究过程中取得了重要进展,随着多项临床试验的结束,已有临床报道提示间质干细胞在修复脊髓损伤的临床转化方面具有良好的前景,同时也对其临床转化提出了更深层、更具体的思考和要求。本文将基于近三年内最新报道的文献和笔者研究团队的近期研究成果,对间质干细胞移植修复脊髓损伤从基础研究到临床转化以及其发展前景做一系统综述。 | 张超 Chekhonin VP Blyukhovetskiy AS 李波 冯世庆 | 2016 | 中华骨科杂志2016，36，6： | 6 |
| 2 | 应用磁共振扩散张量成像技术诊断大鼠创伤后脊髓空洞症显示文摘创伤后脊髓空洞症(PTS)是脊髓损伤(SCI)后髓内囊性退变。PTS可加剧SCI后症状。传统磁共振扫描(MRI)难以对PTS进行早期诊断。磁共振弥散张量成像(DTI)已应用于SCI研究。我们基于7.0TMRI通过DTI分析大鼠SCI模型,评估DTI早期诊断PTS可行性。 | 陈凯 张超 李之珺 付嘉园圆 Vladimir P. Chekhonin | 2018 | 中华实验外科杂志2018，35，7： | 2 |
| 3 | Hydraulic fracture propagation in highly permeable formations, with applications to tip screenout显示文摘 | Chekhonin E Levonyan K | 2012 | International journal of rock mechanics & mining sciences2012，50，2012： | 1 |
| 4 | Targeted delivery of liposomal nanocontainers tothe peritumoral zone of glioma by means of monoclonal anti-bodies against GFAP and the extracellular loop of Cx43 显示文摘 | CHEKHONIN V P BAKLAUSHEV V P YUSUBALIEVAG M | 2012 | Nanomedicine2012，8，1： | 1 |
| 5 | Targeted delivery of liposomal nanocontainers to the peritumoral zone of glioma by means of monoclonal antibodies against GFAP and the extracellular loop ofCx43显示文摘 | Chekhonin VP Baklaushev VP Yusubalieva GM | 2012 | Nanomedicine2012，8，1： | 1 |
| 6 | Myelin basic protein structure,function and role in diagnosing demyelinating disease显示文摘 | Chekhonin VP Gurina OL Dmitrieva TB | | 0，，06： | 1 |
| 7 | Hydraulic fracture propagation in highly permeable formations,with applications to tip screenout显示文摘 | CHEKHONIN E LEVONYAN K | 2012 | International Journal of Rock Mechanics and Mining Sciences2012，50，： | 1 |
| 8 | Myelin oligodendrogliocyte glycoprotein: the structure, functions, role in pathogenesis of demyelinating disorders 显示文摘 | Chekhonin VP Semenova AV Gurina OI | 2003 | Biomed Khim2003，，5： | 1 |
| 9 | VEGF in tumor progression and targeted therapy 显示文摘 | Chekhonin VP Shein SA Korchagina A A | 2013 | Curr Cancer Drug Tar- gets2013，13，4： | 1 |
| 10 | Activation of expression of brain-derived neurotrophic factor at the site of implantation of allogenic and xenogenic neural stem(progenitor)cells in rats with ischemic cortical stroke显示文摘 | Chekhonin VP Lebedev SV Volkov AI | | 0，，： | 1 |
| 11 | Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes显示文摘 | Chekhonin VP Zhirkov IuA Gurina OI | 2005 | Biomed Khim2005，51，3： | 1 |
| 12 | Improvement in negative symptoms of schizophrenia with antibodies to tumor necrosis factor-alpha and to interferon-gamma:a case report显示文摘 | Skurkovich SV Aleksandrovsky YA Chekhonin VP | 2003 | J Clin Psychiatry2003，64，： | 1 |
| 13 | Activation of expression of brain-derived neurotrophic factor at the site of implantation of allogenic and xenogenic neural stem (progenitor) cells in rats with ischemic cortical stroke显示文摘 | CHEKHONIN VP LEBEDEV SV VOLKOV AI | 2011 | Bull Exp Bid Med2011，150，4： | 1 |
| 14 | Targeted transport of 1251-labeled antibody to GFAP and AMVB1 in an experimental rat model of C6 glioma显示文摘 | Chekhonin VP Baklaushev VP Yusubalieva GM | 2009 | J Neuroimmune Pharmacol2009，4，1： | 1 |
| 15 | Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates显示文摘BACKGROUND The development of regenerative therapy for human spinal cord injury(SCI)is dramatically restricted by two main challenges:the need for a safe source of functionally active and reproducible neural stem cells and the need of adequate animal models for preclinical testing.Direct reprogramming of somatic cells into neuronal and glial precursors might be a promising solution to the first challenge.The use of non-human primates for preclinical studies exploring new treatment paradigms in SCI results in data with more translational relevance to human SCI.AIM To investigate the safety and efficacy of intraspinal transplantation of directly reprogrammed neural precursor cells(drNPCs).METHODS Seven non-human primates with verified complete thoracic SCI were divided into two groups:drNPC group(n=4)was subjected to intraspinal transplantation of 5 million drNPCs rostral and caudal to the lesion site 2 wk post injury,and lesion control(n=3)was injected identically with the equivalent volume of vehicle.RESULTS Follow-up for 12 wk revealed that animals in the drNPC group demonstrated a significant recovery of the paralyzed hindlimb as well as recovery of somatosensory evoked potential and motor evoked potential of injured pathways.Magnetic resonance diffusion tensor imaging data confirmed the intraspinal transplantation of drNPCs did not adversely affect the morphology of the central nervous system or cerebrospinal fluid circulation.Subsequent immunohistochemical analysis showed that drNPCs maintained SOX2 expression characteristic of multipotency in the transplanted spinal cord for at least 12 wk,migrating to areas of axon growth cones.CONCLUSION Our data demonstrated that drNPC transplantation was safe and contributed to improvement of spinal cord function after acute SCI,based on neurological status assessment and neurophysiological recovery within 12 wk after transplantation.The functional improvement described was not associated with neuronal differentiation of the allogeneic drNPCs.Instead,directed drNPCs migration to the areas of active growth cone formation may provide exosome and paracrine trophic support,thereby further supporting the regeneration processes. | Vladimir P Baklaushev Oleg V Durov Vladimir A Kalsin Eugene V Gulaev Sergey V Kim Ilya L Gubskiy Veronika A Revkova Ekaterina M Samoilova Pavel A Melnikov Dzhina D Karal-Ogly Sergey V Orlov Alexander V Troitskiy Vladimir P Chekhonin Alexander V Averyanov Jan-Eric Ahlfors | 2021 | World Journal of Stem Cells2021，13，5： | 1 |
| 16 | Genetic determinants of aggression and impulsivity in hu- mans显示文摘 | Pavlov KA Chistiakov DA Chekhonin VP | 2012 | J Appl Genet2012，53，1： | 1 |
| 17 | Role of microRNAs in mechanisms of glioblastoma resistance to radio- and chemotherapy显示文摘 | Koshkin PA Chistiakov DA Chekhonin VP | 2013 | Biochemistry(Mosc)2013，78，4： | 1 |
| 18 | Genetic determinants of ag-gression and impulsivity in humans显示文摘 | Pavlov KA Chistiakov DA Chekhonin VP | 2012 | J Appl Genet2012，53，： | 1 |
| 19 | VEGF in tumor progression and targeted therapy 显示文摘 | Chekhonin VP Shein SA Korchagina A A | 2013 | Curr Cancer Drug Targets2013，13，4： | 1 |
| 20 | Myelin oli- godendroglioeyte glycoprotein: the structure, functions, role in pathogenesis of demyelinating disorders 显示文摘 | Chekhonin VP Semenova AV Gurina OI etal | 2003 | Biomed Khim2003，5，： | 1 |
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