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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Evolution and advances in laparoscopic ventral and incisional hernia repair显示文摘Primary ventral hernias and ventral incisional hernias have been a challenge for surgeons throughout the ages. In the current era, incisional hernias have increased in prevalence due to the very high number of laparotomies performed in the 20 th century. Even though minimally invasive surgery and hernia repair have evolved rapidly, general surgeons have yet to develop the ideal, standardized method that adequately decreases common postoperative complications, such as wound failure, hernia recurrence and pain. The evolution of laparoscopy and ventral hernia repair will be reviewed, from the rectoscopy of the 4th century to the advent of laparoscopy, from suture repair to the evolution of mesh reinforcement. The nuances of minimally invasive ventral and incisional hernia repair will be summarized, from preoperative considerations to variations in intraoperative practice. New techniques have become increasingly popular, such as primary defect closure, retrorectus mesh placement, and concomitant component separation. The advent of robotics has made some of these repairs more feasible, but only time and well-designed clinical studies will tell if this will be a durable modality for ventral and incisional hernia repair. | Alan L Vorst Christodoulos Kaoutzanis Alfredo M Carbonell Michael G Franz | 2015 | World Journal of Gastrointestinal Surgery2015，7，11： | 10 |
| 3 | Role of aldehyde dehydrogenase 2 in ischemia reperfusion injury:An update显示文摘Aldehyde dehydrogenase 2(ALDH2) is best known for its critical detoxifying role in liver alcohol metabolism. However, ALDH2 dysfunction is also involved in a wide range of human pathophysiological situations and is associated with complications such as cardiovascular diseases, diabetes mellitus, neurodegenerative diseases and aging. A growing body of research has shown that ALDH2 provides important protection against oxidative stress and the subsequent loading of toxic aldehydes such as 4-hydroxy-2-nonenal and adducts that occur in human diseases, including ischemia reperfusion injury(IRI). There is increasing evidence of its role in IRI pathophysiology in organs such as heart, brain, small intestine and kidney; however, surprisingly few studies have been carried out in the liver, where ALDH2 is found in abundance. This study reviews the role of ALDH2 in modulating the pathways involved in the pathophysiology of IRI associated with oxidative stress, autophagy and apoptosis. Special emphasis is placed on the role of ALDH2 in different organs, on therapeutic 'preconditioning' strategies, and on the use of ALDH2 agonists such as Alda-1, which may become a useful therapeutic tool for preventing the deleterious effects of IRI in organ transplantation. | Arnau Panisello-Roselló Alexandre Lopez Emma Folch-Puy Teresa Carbonell Anabela Rolo Carlos Palmeira René Adam Marc Net Joan Roselló-Catafau | 2018 | World Journal of Gastroenterology2018，24，27： | 6 |
| 4 | Ubiquitin-proteasome system and oxidative stress in liver transplantation显示文摘A major issue in organ transplantation is the development of a protocol that can preserve organs under optimal conditions. Damage to organs is commonly a consequence of flow deprivation and oxygen starvation following the restoration of blood flow and reoxygenation. This is known as ischemia-reperfusion injury(IRI): a complex multifactorial process that causes cell damage. While the oxygen deprivation due to ischemia depletes cell energy, subsequent tissue oxygenation due to reperfusion induces many cascades, from reactive oxygen species production to apoptosis initiation. Autophagy has also been identified in the pathogenesis of IRI, although such alterations and their subsequent functional significance are controversial. Moreover, proteasome activation may be a relevant pathophysiological mechanism. Different strategies have been adopted to limit IRI damage, including the supplementation of commercial preservation media with pharmacological agents or additives. In this review, we focus on novel strategies related to the ubiquitin proteasome system and oxidative stress inhibition, which have been used to minimize damage in liver transplantation. | Norma Alva Arnau Panisello-Roselló Marta Flores Joan Roselló-Catafau Teresa Carbonell | 2018 | World Journal of Gastroenterology2018，24，31： | 5 |
| 5 | Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal显示文摘AIM To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin(UW) and Institut Georges Lopez-1(IGL-1)solutions.METHODS Fatty liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 ℃and subjected to 'ex vivo ' normo-thermic perfusion(2 h; 37 ℃). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system(UPS: measured as chymotryptic-like activity and 20 S and 19 S proteasome), the prevention of liver injury(transaminases), mitochondrial injury(confocal microscopy) and inflammation markers(TNF 1 alpha, high mobility group box-1(HGMB-1) and PPAR gamma), and liver apoptosis(TUNEL assay, cytochrome c and caspase 3).RESULTS Profiles of free AA(alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW(P < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity(measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury(AST/ALT) and mitochondrial damage(revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers(TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 levels. CONCLUSION Our comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a higher UPS inhibition and reduced liver proteolysis. | Mohamed Amine Zaouali Arnau Panisello-Roselló Alexandre Lopez Carlos Castro Benítez Emma Folch-Puy Agustín García-Gil Teresa Carbonell RenéAdam Joan Roselló-Catafau | 2017 | World Journal of Gastroenterology2017，23，23： | 3 |
| 6 | Bortezomib enhances fatty liver preservation in I nstitut G eorge L opez‐1 solution through adenosine monophosphate activated protein kinase and Akt / mTOR pathways显示文摘 | Mohamed Bejaoui Mohamed Amine Zaouali Emma Folch‐Puy Eirini Pantazi Fawzia Bardag‐Gorce Teresa Carbonell Joan Oliva Antoni Rimola Hassen Ben Abdennebi Joan Roselló‐Catafau | 2014 | J Pharm Pharmacol2014，，1： | 3 |
| 7 | Approach to early-onset colorectal cancer:Clinicopathological,familial,molecular and immunohistochemical characteristics显示文摘AIM:To characterize clinicopathological and familial features of early-onset colorectal cancer(CRC) and compare features of tumors with and without microsatellite instability(MSI).METHODS:Forty-five patients with CRC aged 45 or younger were included in the study.Clinical information,a three-generation family history,and tumor samples were obtained.MSI status was analyzed and mismatch repair genes were examined in the MSI families.Tumors were included in a tissue microarray and an immunohistochemical study was carried out with a panel of selected antibodies.RESULTS:Early onset CRC is characterized by advanced stage at diagnosis,right colon location,low-grade of differentiation,mucin production,and presence of polyps.Hereditary forms represent at least 21% of cases.Eighty-one percent of patients who died during followup showed a lack of expression of cyclin E,which could be a marker of poor prognosis.β-catenin expression was normal in a high percentage of tumors.CONCLUSION:Early-onset CRC has an important familial component,with a high proportion of tumors showing microsatellite stable.Cyclin E might be a poor prognosis factor. | Jose Perea Edurne Alvaro Yolanda Rodríguez Cristina Gravalos Eva Sánchez-Tomé Barbara Rivera Francisco Colina Pablo Carbonell Rogelio González-Sarmiento Manuel Hidalgo Miguel Urioste | 2010 | World Journal of Gastroenterology2010，16，29： | 3 |
| 8 | Polyethylene glycols: An effective strategy for limiting liver ischemia reperfusion injury显示文摘Liver ischemia-reperfusion injury(IRI) is an inherent feature of liver surgery and liver transplantation in which damage to a hypoxic organ(ischemia) is exacerbated following the return of oxygen delivery(reperfusion). IRI is a major cause of primary nonfunction after transplantation and may lead to graft rejection, regardless of immunological considerations. The immediate response involves the disruption of cellular mitochondrial oxidative phosphorylation and the accumulation of metabolic intermediates during the ischemic period, and oxidative stress during blood flow restoration. Moreover, a complex cascade of inflammatory mediators is generated during reperfusion, contributing to the extension of the damage and finally to organ failure. A variety of pharmacological interventions(antioxidants, anticytokines, etc.) have been proposed to alleviate graft injury but their usefulness is limited by the local and specific action of the drugs and by their potential undesirable toxic effects. Polyethylene glycols(PEGs), which are non-toxic water-soluble compounds approved by the FDA, have been widely used as a vehicle or a base in food, cosmetics and pharmaceuticals, and also as adjuvants for ameliorating drug pharmacokinetics. Some PEGs are also currently used as additives in organ preservation solutions prior to transplantation in order to limit the damage associated with cold ischemia reperfusion. More recently, the administration of PEGs of different molecular weights by intravenous injection has emerged as a new therapeutic tool to protect liver grafts from IRI. In this review, we summarize the current knowledge concerning the use of PEGs as a useful target for limiting liver IRI. | Gianfranco Pasut Arnau Panisello Emma Folch-Puy Alexandre Lopez Carlos Castro-Benítez Maria Calvo Teresa Carbonell Agustín García-Gil RenéAdam Joan Roselló-Catafau | 2016 | World Journal of Gastroenterology2016，22，28： | 3 |
| 9 | Milliwatt terahertz harmonic generation from topological insulator metamaterials显示文摘Achieving effcient,high-power harmonic generation in the terahertz spectral domain has technological applications,for example,in sixth generation(6G)communication networks.Massless Dirac fermions possess extremely large terahertz nonlinear susceptibilities and harmonic conversion effciencies.However,the observed maximum generated harmonic power is limited,because of saturation effects at increasing incident powers,as shown recently for graphene.Here,we demonstrate room-temperature terahertz harmonic generation in a Bi_(2)Se_(3) topological insulator and topological-insulator-grating metamaterial structures with surface-selective terahertz field enhancement.We obtain a third-harmonic power approaching the miliwatt range for an incident power of 75 mW-an improvement by two orders of magnitude compared to a benchmarked graphene sample.We establish a framework in which this exceptional performance is the result of thermodynamic harmonic generation by the massless topological surface states,benefiting from ultrafast dissipation of electronic heat via surface-bulk Coulomb interactions.These results are an important step towards on-chip terahertz(opto)electronic applications. | Klaas-Jan Tielrooij Alessandro Principi David Saleta Reig Alexander Block Sebin Varghese Steffen Schreyeck Karl Brunner Grzegorz Karczewski Igor Ilyakov Oleksiy Ponomaryov Thales V.A.G.de Oliveira Min Chen Jan-Christoph Deinert Carmen Gomez Carbonell Sergio O.Valenzuela Laurens W.Molenkamp Tobias Kiessling Georgy V.Astakhov Sergey Kovalev | 2022 | Light(Science & Applications)2022，11，11： | 2 |
| 10 | Laparoscopic mesh hiatoplasty for paraesophageal hernias and fundoplieations: a critical analysis of the available literature显示文摘 | Johnson JM Carbonell AM Carmody B J | 2006 | Surg Endose2006，20，3： | 1 |
| 11 | Iron phthalocyanine supported on silica or encapsulated inside zeolite Y as solid photocatalysts for the degradation of phenols and sulfurheterocycles 显示文摘 | Alvaro M Carbonell E Espla M | 2005 | Appl Catal B: Env2005，57，： | 1 |
| 12 | Successful correction of the human beta-thalassemia major phenotype using a lentiviral vector显示文摘 | Puthenveetll G ScholesJ Carbonell D | 2004 | Blood2004，104，12： | 1 |
| 13 | Determination of polystyrene-carbon dioxide- decahydronaphthalene solution properties by high pressure dynamic light scattering显示文摘 | Dong L B Carbonell R G Roberts G W | 2009 | Polymer2009，50，24： | 1 |
| 14 | Improved survival after variceal bleeding in patients with cirrhosis over the past two decades显示文摘 | CARBONELL N PAUWELS A SERFATY L | 2004 | Hepatology2004，40，3： | 1 |
| 15 | Ajugatansins,neo-clerodane diterpenes from Ajuga reptans显示文摘 | Carbonell P Coll J | 2001 | Phytochem Anal2001，12，1： | 1 |
| 16 | Phase H study of efficacy, safety, and pharmacokinetics of trastuzumab monotherapy administered on a 3-Weekly Schedule 显示文摘 | Baselga J Carbonell X Castaneda-Soto NJ | 2005 | J CIin Oncol2005，23，10： | 1 |
| 17 | Evaluation of groundwater con- tamination beneath an urban environment: the Besbs River basin ( Barcelona, Spain) 显示文摘 | NAVARRO A CARBONELL M | 2007 | Journal of Environmental Management2007，85，2： | 1 |
| 18 | Palivizumab prophylaxis, respiratory syncytial virus, and subsequent recurrent wheezing显示文摘 | Simoes EA Groothuis JR Carbonell - Estrany X | 2007 | J Pediatr2007，151，1： | 1 |
| 19 | Bird conservation in fragmented Mediterranean forests of Spain: effects of geographical location, habitat and landscape degradation 显示文摘 | Santos T Telleria J L Carbonell R | 2002 | Biological Conserv- ation2002，105，： | 1 |
| 20 | Arginine decarboxylase and putrescine oxidase in ovaries of Pisum sativum L显示文摘 | MIGUEL A PEREZ A CARBONELL J | 1995 | Plant Physiol1995，107，： | 1 |
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