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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Effect of a fixed-ratio(1∶1∶1)transfusion protocol versus laboratory-results-guided transfusion in patients with severe trauma:a randomized feasibility trial显示文摘 | NASCIMENTO B CALLUM J TIEN H | 2013 | CMAJ2013，185，： | 1 |
| 3 | Blind deconvolution by simulated annealing显示文摘 | Callum B M | 1990 | Optics Communications1990，75，2： | 1 |
| 4 | Blind deconvolulion by simulated annealing显示文摘 | CALLUM B M | 1990 | Optics Communications1990，75，2： | 1 |
| 5 | A novel DNAbinding protein modulating methicillin resistance in staphylococcus aureus显示文摘 | Ender M Berger-Bchi B Mc Callum N | 2009 | BMC Microbiol2009，9，1： | 1 |
| 6 | An approach to transfusion and hemorrhage in trauma:current perspectives on restrictive transfusion strategies显示文摘 | Tien H Nascimento B Callum J | 2007 | Can J Surg2007，50，： | 1 |
| 7 | Painful neuropathy alters the effect of gabapentin on sensory neuron exeitability in rats 显示文摘 | Kanai A Sarantopoulos C Mc Callum J B | 2004 | Acta Anaesthesiol Seand2004，48，4： | 1 |
| 8 | Effect of a fixed-ratio (1:1:1) transfusion protocol versus laboratory-results-guided transfusion in patients with severe trauma:a randomized feasibility trial显示文摘 | Nascimento B Callum J Tien H | | 0，，： | 1 |
| 9 | Effect of a fixed-ratio (1 ∶ 1 ∶ 1) transfusion protocol versus laboratory-resultsguided transfusion in patients with severe trauma:a randomized feasibility trial显示文摘 | Nascimento B Callum J Tien H | 2013 | CMAJ2013，185，12： | 1 |
| 10 | Contributions of nitrogen by field pea (Pisum sativum) in a continuous cropping sequence compared with a Lucerne (Medicago sativa)-based pasture ley in the Victorian Wimmera显示文摘 | Ma Callum M H Peoples M B Connor D J | 2000 | Australian Journal of Agricultural Research2000，51，： | 1 |
| 11 | Contributions of nitrogen by field pea (Pisum sativum) in a continuous cropping sequence compared with a Lucerne (Medicago sativa) based pastureley in the Victorian Wimmera显示文摘 | Ma Callum M H Peoples M B Connor D J | 2000 | Australian Journal of Agricultural Research2000，51，： | 1 |
| 12 | Vitamin K-dependent coagulation factor deficiency in trauma:a comparative analysis between international normalized ratio and thromboelastogra- phy显示文摘 | Naimento B AI Mahoos M Callum J | 2012 | Transfusion2012，52，1： | 1 |
| 13 | An approach to transfusion and hemorrhage in trauma: current perspectives on restrictive transfusion strategies 显示文摘 | Tien H Nascimento B Jr Callum J | 2007 | Can J Surg2007，50，3： | 1 |
| 14 | Vitamin K-depend- ent coagulation factor deficiency in trauma : A comparative analysis between international normalized ratio and thromboelastography 显示文摘 | Nascimento B A1 Mahoos M Callum J | 2012 | Transfusion2012，52，1： | 1 |
| 15 | Host-induced gene silencing of wheat leaf rust fungus Puccinia tritici- na pathogenicity genes mediated by the Barley stripe mo- saic virus显示文摘 | Panwar V Mc Callum B Bakkeren G | 2013 | Plant Mol Biol2013，81，： | 1 |
| 16 | Drems,inages and e- motions associated with propofol anaesthesia 显示文摘 | Brander B Blagrove M Mc Callum G | 1997 | Anaesthesia1997，52，8： | 1 |
| 17 | Blind deconvolution by simulated annealing显示文摘 | Callum B M | 1990 | Optics Communications1990，75，2： | 1 |
| 18 | Dreams, inages and emotions associated with ProPofol anaesthesia显示文摘 | Brander B Blagrove M Mc Callum G | 1997 | Anaesthesia1997，52，8： | 1 |
| 19 | Vitamin K de- pendent coagulation factor deficiency in trauma:A compara- tive analysis between international normalized rationand thromboelastography显示文摘 | Nascimento B A1 Mahoos M Callum J | 2012 | Transfusion2012，52，1： | 1 |
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