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| 1 | OPS法玻璃化冷冻牛卵母细胞和囊胚显示文摘本文报道了利用拉细开口型细管 (Open Pulled Straw,OPS)方法进行玻璃化冷冻保存体外成熟(IVM)的牛卵母细胞及体外生产 (IVP)的牛囊胚的实验结果。实验 1的牛卵母细胞冷冻处理分 2组 ,组 1(G1 )是将卵母细胞在 IVM2 1 h时去除部分卵丘细胞后马上进行冷冻处理。组 2 (G2 )卵母细胞在 IVM6h时去除部分卵丘细胞 ,然后继续培养至 2 2 h冷冻 ;冷冻后的卵母细胞在液氮中保存 0 .5~ 2 h后解冻再继续 IVM1 h后与正常 IVM2 4h的对照组卵母细胞一起进行体外受精 (IVF)处理。实验 2则冷冻了来自IVP的 6、 7日龄的牛囊胚。结果显示 ,G1的卵母细胞 IVF后 8d的囊胚率 (2 2 .8% ,2 6/1 1 4)和孵化囊胚率 (1 4.9% ,1 7/1 1 4)都极显著地高于 G2 (分别为 2 .9% ,5 /1 71和 0 .5 % ,1 /1 71 ,P<0 .0 0 1 ) ;但两处理组的受精卵裂率差别不大 (分别为 48.2 %和 41 .5 % ,P>0 .3 )。冷冻第 6d和第 7d的体外囊胚 ,其冻后存活率和囊胚孵化率分别为 92 .7%、 89.2 %和 83 .6%、 66.7%。结果表明 ,利用 | 谭世俭 Henrik Callesen | 2002 | 广西农业生物科学2002，21，1： | 19 |
| 2 | 有无透明带牛卵母细胞孤雌发育研究显示文摘研究探讨了不同浓度的钙离子载体 (A2 3187)结合 6 二甲氨基嘌呤 (DMAP)或丁内酯I (BL -I)激活处理对有或无透明带牛卵母细胞孤雌发育的影响。结果表明 :使用A2 3187浓度从 0 .6 2 5~ 10 .0 0 0 μmol/L配合 2 .0mmol/LDMAP孤雌激活牛具透明带卵均能获得良好的卵裂及早期胚胎发育效果 ;对于去透明带卵来说 ,使用 0 .6 2 5 μmol/L浓度的A2 3187激活效果最佳。BL I的激活效果则明显差于DMAP ;当采用 0 .3%PVA取代激活液中的 5 %血清时 ,BL I的激活效率显著提高。 | 谭世俭 P J Booth H Callesen | 2004 | 黑龙江畜牧兽医2004，，2： | 4 |
| 3 | Open Pulled Straw(OPS) Vitrifica- tion: a New Way to Reduce Cryoinjuries of Bovine Ova and Embryos 显示文摘 | Vajta G Holm P Kuwayama M Booth P J Jacobsen H Greve T Callesen H | 1998 | Molecular Reproduction and Develop- ment1998，51，1： | 1 |
| 4 | Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin显示文摘 | Ying Liu Olga ?strup Juan Li Gábor Vajta Lin Lin Peter M. Kragh Stig Purup Poul Hyttel Henrik Callesen | 2011 | Zygote2011，，1： | 1 |
| 5 | Developmental competence of HMC (TM) derived bovine cloned embryos obtained from somatic cell nuclear transfer of adult fibroblasts and granulose cells 显示文摘 | BHOJWANI S VAJTA G CALLESEN H | 2005 | Theriogenology2005，64，： | 1 |
| 6 | Nitrate leaching in forest ecosystems is controlled by forest floor C /N ratio 显示文摘 | Gundersen P Callesen I de Vries W | 1998 | Environmental Pollution1998，102，1： | 1 |
| 7 | Treatment with the long-acting insulin analogues detemir or glargine during preg- nancy in women with type 1 diabetes:comparison of glycaemic control and pregnancy outcome 显示文摘 | Callesen NF Damm J Mathiesen JM | 2013 | J Matem Fetal Neonatal Med2013，26，6： | 1 |
| 8 | Treatment with the long- acting insulin analogues detemir or glargine during pregnancy in women with type 1 diabetes: comparison of glycaemic control and pregnancy outcome显示文摘 | Callesen NF Damm J Mathiesen JM | 2013 | J Matern Fetal Neonatal Med2013，26，6： | 1 |
| 9 | The nutrient status of Norway spruce in pure and in mixedspecies stands 显示文摘 | Thelin G Rosengren U Callesen I | 2002 | Forest Ecology and Management2002，160，： | 1 |
| 10 | Carbon and nitrogen in forest floor and mineral soil under six com- mon European tree species 显示文摘 | Vesterdal L Schmidt I K Callesen I | 2008 | Forest Ecology and Management2008，255，1： | 1 |
| 11 | Diabetic Nephropathy and Microalbuminuria in pregnant women with type 1 and type 2 diabetes:prevalence,antihypertensive strategy,and pregnancy outcome显示文摘 | J A Damm B Asbjrnsdóttir NF Callesen | 2013 | Diabetes Care2013，36，11： | 1 |
| 12 | Vitrification of bovine oocytes with the open pulled straw method: ultrastruetural consequences 显示文摘 | Hyttel P Vajta G Callesen H | 2000 | Mol Reprod Dev2000，56，： | 1 |
| 13 | Prospective study of chronic pain after groin hernia repair显示文摘 | Callesen T Bech K Kehlet H | 1999 | Br J Surg1999，86，12： | 1 |
| 14 | Carbon and Nitrogen in Danish Forest Soils--Contents and Distribution Determined by Soil Or der显示文摘 | Vejre H Y Callesen I Vesterdal L | 2003 | Soil Science Society of America Journal2003，67，11： | 1 |
| 15 | Diabetic nephropathy and microalbuminuria in pregnant women with type 1 and type 2 diabetes:prevalence,antihypertensive strategy,and pregnancy outcome显示文摘 | Damm JA Asbj(o)rnsdóttir B Callesen NF | 2013 | Diabetes Care2013，36，11： | 1 |
| 16 | One-thousand consecutive inguinal hernia repairs under unmonitored local anesthesia显示文摘 | Callesen T Bech K Kehlet H | 2001 | Anesth Analg2001，93，3： | 1 |
| 17 | Light distribution in apple or chard system in relation to production and fruit quality显示文摘 | Wagenmaker P Callesen Q | 1995 | Journal of Horticultural Science1995，70，： | 1 |
| 18 | Soil respiration and rates of soil carbon turnover differ among six common European tree species显示文摘 | Lars Vesterdal Bo Elberling Jesper Riis Christiansen Ingeborg Callesen Inger Kappel Schmidt | 2011 | Forest Ecology and Management2011，，： | 1 |
| 19 | Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the Handmade Cloning (HMC) and the Submarine Incubation System (SIS)显示文摘 | Gábor Vajta Paul Bartels Jennifer Joubert Morné de la Rey Robert Treadwell Henrik Callesen | 2004 | Theriogenology2004，，8： | 1 |
| 20 | Recovery at the post anaesthetic care unit after breast cancer surgery显示文摘 | Gartner R Callesen T Kroman N | 2010 | Dan Med Bull2010，57，2： | 1 |
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